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Citations for abcam :
4901 - 4950 of 5055 citations for Recombinant Mouse Il13ra1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with primary antibodies diluted in blocking buffer overnight at 4°C - Mouse Anti-Human TRA1-81 (1:75, Abcam, AB16289#20), Rabbit Anti-Human OCT4 (1:250 ...
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bioRxiv - Physiology 2023Quote: ... Adjacent sections were used for immunohistochemistry with anti-VDR (1:100, rat) and anti-AMH (1:50, mouse) monoclonal antibodies (Abcam, Cambridge, UK) as per manufacturers’ protocol with modifications.
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bioRxiv - Physiology 2023Quote: ... with the primary antibody goat anti-HIF-1α (1:100, SicGen, Cantanhede, Portugal) and mouse anti-HIF-2α (1:100, Abcam, Cambridge, UK). After washing the slices were incubated with secondary antibody donkey anti-goat Alexa Fluor 546 (1:5000 ...
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bioRxiv - Physiology 2023Quote: ... Solution was decanted and slices washed in TBS-T before being incubated with specific secondary antibody in a dilution of 1:2000 for 1h at 24°C in the dark (anti-rabbit Alexa Fluor 550 or anti-mouse Alexa Fluor 488; from Abcam, Cambridge, UK). Slices were washed in TBS in the dark and mounted with a coverslip and a drop of mounting medium Fluoroshield™ - Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... cells were blocked for 1 h in 1×PBS + 2% w/v BSA and then incubated with mouse anti-E-cadherin (1:1000) (Abcam, cat#231303) and chicken anti-vimentin (1:200 ...
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bioRxiv - Biochemistry 2023Quote: The following secondary antibodies have been used for Western blotting: Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Abcam, #ab7068; 1:10000), Recombinant Protein G (HRP ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated for 2 h with 700 µL of a 1/250 dilution of anti-mouse IgG Alexa FluorTM 555 secondary antibody (ab150106; Abcam, Cambridge, UK) in MTSB + 5% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The macrophages in the atherosclerosis in the thoracoabdominal aorta of the rabbits were revealed using mouse anti-rabbit CD68 antibody (Abcam, Cambridge, UK) as the primary antibody and goat anti-mouse IgG antibody (horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2022Quote: ... Rabbit monoclonal IgG antibodies against MMP-9 and Cx43 and CD31 and a mouse IgG antibody against Rac-1 were purchased from Abcam (Cambridge, UK). Rabbit polyclonal IgG antibodies against Na+/K+-ATPase and CD68 were purchased from Arigo (Hsinchu ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained for 30 min with polyclonal fluorescein-isothiocyanate (FITC)-conjugated goat anti-mouse IgG H&L specific antibody (Abcam, United Kingdom) diluted 1:50 in RS-BSA 0.1% ...
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bioRxiv - Genetics 2023Quote: ... The chromatin was purified through centrifugation and immunoprecipitated using Dynabeads M-280 Sheep anti-mouse IgG (Veritas Life Sciences, PA, USA) conjugated with anti-H3K36me2 antibodies (ab176921; Abcam, Cambridge, UK) in 1× RIPA (150 mM ...
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bioRxiv - Immunology 2023Quote: ... The following primary antibodies were used for the western blot experiments: mouse monoclonal antibody against human apolipoprotein E (dilution 1:1000; Abcam, Cambridge, UK), and polyclonal rabbit antibodies against the LL37 epitope (diluted 1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse monoclonal Abs (mMAbs) against lamin A/C C-terminus residues aa 319-566 (LA/C-C, [131C3], ab18984, Abcam, Cambridge, UK) and lamin A/C N-terminus residues aa 2-29 (LA/C-N ...
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bioRxiv - Biochemistry 2023Quote: ... The grids were then washed with PBS+ three times for 2 min and incubated on a 20 ul drop of secondary antibodies conjugated with gold particles (1:20 dilution of anti-mouse and anti-rabbit secondary antibodies conjugated with 10nm and 6nm gold particles respectively, Abcam, Cambridge, UK). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... and subsequently incubated for 1 h with a 1:5000 dilution of a horseradish peroxidase-conjugated goat anti-mouse secondary antibody (ab6789, Abcam, Cambridge, UK) or goat anti-rabbit secondary antibody (ab6721 ...
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bioRxiv - Immunology 2023Quote: ... as described previously.25 IgA levels in lung homogenates were measured by ELISA with goat anti-mouse IgA a chain antibody conjugated to horseradish peroxidase (ab97235; Abcam, Cambridge, UK), as previously reported.10 MPO activity was measured in lung homogenates as reported previously25 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed twice with PBST before being incubated with the primary antibody (Mouse anti-BrdU, 1:1000, Cat. No. B35128, Invitrogen; or Rat anti-BrdU, 1:1000, Cat. No. ab6326, Abcam, RRID: AB_305426) and the secondary antibody (Donkey anti-mouse 488 or 568 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at 4°C overnight with primary antibodies including: NeuN (1:500, mouse, Millipore, Cat#:MAB377; 1:500, rabbit, Abcam, Cat#: ab104225), MBP (1:300 ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... The samples were incubated at 4℃ for 48 hours with mouse anti-CaMKIIα antibodies (ab22609, abcam, 1:250 dilution in 2% NGS). After treating the primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... The macrophages in the plaque in the thoracoabdominal aorta of the rabbits were revealed using mouse anti-rabbit CD16/32 and CD206 antibody (Abcam, Cambridge, UK) as the primary antibody and goat anti-mouse IgG antibody (HRP labeled ...
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bioRxiv - Bioengineering 2023Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Thermo Fisher, N-21479, donkey anti-rabbit 488, Abcam, ab150061, donkey anti-mouse 568, Abcam, ab175700, donkey anti-goat 647, Abcam, ab150135) in concentration of 1:500 were applied ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with goat-anti-mouse IgG secondary antibody conjugated with horseradish peroxidase (ab6789, Abcam, Cambridge, UK; 2 mg/ml) at a final concentration of 0.8 μg/ml / in 1:2500 dilution in milk blocking buffer for 1.5 hours at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with 5% BSA in TBS-T for 1h before overnight incubation with rabbit anti-FXR (H-130; 1:2.000) and mouse anti-β-actin (ab6276, Abcam; 1:10.000) followed by incubation with goat anti-rabbit-IgG-AlexaFluor 647 and goat anti-mouse-IgG-AlexaFluor 488 (both at 1:5.000) ...
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bioRxiv - Genetics 2024Quote: ... excluding the step of 2M HCL incubation and by using 1/200 primary antibody targeting XPC (mouse, Santa Cruz, sc-74410 or vimentin (rabbit, Abcam, ab92547).
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bioRxiv - Developmental Biology 2024Quote: ... the tissue sections were incubated with primary antibodies including α-TECTA produced in rabbit (1:1000) or mouse α–Col II (1:100; ab150771, Abcam)) at 4°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... using a solution containing goat polyclonal secondary antibody to mouse or rabbit IgG – H&L conjugated to Alexa Fluor 488 (Abcam AB150113, AB150077), added in a 1:100 dilution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the coverslips were incubated overnight (4 °C) with a mouse monoclonal anti-15-LOX-1 antibody (1:100, # ab119774, Lot: GR3322776-8; Abcam, Cambridge, UK), washed repeatedly ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed twice with PBS before incubation overnight at 4°C in primary mouse IgG2b anti-NeuN (1:500, Abcam cat# ab104224). Sections were washed twice the following day with 1X PBS followed by incubation in goat anti-IgG2b AlexaFlour 647 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated overnight at 4 °C with mouse Anti-GABAAR γ2 primary antibody (1:500; catalog no. ab307231, Abcam, United Kingdom) in a permeable buffer containing 2% donkey serum ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibodies (AF488 goat anti-mouse IgG, Abcam catalog #ab150113; AF594 goat anti-rabbit, Abcam catalog #ab150080; AF680 goat anti-rat IgG, Abcam catalog #ab175778) were diluted 1:500 in DPBS with BSA and Triton-X100 and incubated for 75 minutes at room temperature in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoprecipitation of mGluR2 was performed overnight at 4°C using a mouse monoclonal anti-mGluR2 N-terminal antibody (Abcam, Catalog no. ab15672), followed by RNA isolation ...
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bioRxiv - Neuroscience 2024Quote: ... 0.4% Triton X) before being stained overnight at 4C with the following primary antibodies: mouse anti-APC (CC1) (Abcam #ab16794, 1:100), goat anti-CCL11 (R&DSystems #AF-420-SP ...
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bioRxiv - Molecular Biology 2024Quote: ... washed three times with PBS for and subsequently stained with AF647 anti-mouse secondary antibody (1:200, Abcam, ab169348 in 1% BSA) for 1 h at RT ...
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bioRxiv - Biophysics 2021Quote: ... the cells were blocked for 1 h at room temperature using an unconjugated goat anti-mouse IgG H&L antibody (polyclonal, 1:1000, Abcam ab6708, Cambridge, UK) diluted in previously mentioned blocking solution ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the primary antibody (monoclonal mouse anti-vinculin, 1:100, Sigma V9264, St. Louis, USA; polyclonal rabbit anti-talin 1, 1:100, Abcam ab71333, Cambridge, UK) diluted in blocking solution for 1.5 h at room temperature or overnight at 4 °C.
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bioRxiv - Cell Biology 2020Quote: ... the primary rabbit mono-specific antibodies were diluted into a concentration of 2-4 µg/ml in blocking buffer (PBS + 4% FBS) containing 1 µg/ml mouse anti-alpha-tubulin (Abcam, ab7291, Cambridge, UK) and chicken anti-KDEL respectively (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... This was directly followed by incubation overnight at room temperature with primary antibodies (GFAP; host: mouse; diluted 1:1000; Sigma-Aldrich; Cat# MAB360. GFP; host: chicken; diluted 1:2000; Abcam; cat# ab13970) diluted in antibody solution (ABS ...
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bioRxiv - Molecular Biology 2020Quote: ... Bottom halves of dermal sample membranes were incubated with rabbit anti-TIMP3 (Cell Signalling Technology; D74B10) and top halves with mouse anti-HSA (Abcam, ab10241; loading control). Bottom half of epidermal sample membranes were incubated with rabbit anti-RPL36 (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed 3 times and then stained simultaneously with two secondary antibodies: goat anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1:400 diluition; ab150117; Abcam, Cambridge, UK) and rhodamine red -X-coniugated affinity pure goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2019Quote: ... with 2-8 μg total protein loaded per lane and the following dilutions of mouse mAbs: anti-K14 (1:500; clone LL002, Abcam Inc., Cambridge, UK), anti-K42 (1:500) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the samples were incubated with Alexa Fluor 488- or 594-conjugated goat-anti-mouse or goat-anti-rabbit secondary antibodies (Abcam, 1:500 dilution) overnight at 4 °C and rinsed several times in PBST before mounting in ProLong Diamond Antifade mountant (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Afterward the sections were incubated with 1:100 diluted mouse polyclonal anti-GFAP (Cusabio, Houston, TX, USA) and 1:500 diluted rabbit polyclonal anti-HuC/D (Abcam, Cambridge, United Kingdom) overnight at 4°C· followed by incubation with Cy3-labeled goat anti-mouse IgG (Beyotime ...
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bioRxiv - Cell Biology 2021Quote: ... and for >16 hrs at 4°C with the following primary antibodies: mouse-α-CD63 (1:1000, clone TS63; Abcam, Cambridge, United Kingdom), mouse-α-Flotillin-1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... so membranes were also incubated with a mouse monoclonal anti-beta actin primary antibody (dilution: 1:5000, product number: ab8226, Abcam, Cambridge, MA, USA). After TBST washing three times for 10 minutes each wash ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-CaVβ3 (Alomone Labs #AAC-008, 1:800) or mouse anti-CaVβ4 (Neuromab #75-054, 1:1000) and rabbit anti-β-tubulin (Abcam #ab6046, 1:600,000). Membranes were then incubated in LiCor Blocking Buffer as described above and LiCor IRDye secondary antibodies ...
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bioRxiv - Physiology 2022Quote: Both neonatal and adult ducts were stained using antibodies against the cholangiocyte marker keratin 19 (K19, 1:10, Developmental Studies Hybridoma Bank, TROMAIII) and for the myofibroblast marker α-smooth muscle actin (mouse α-SMA, Abcam ab7817). Adult bile ducts were also stained for hematoxylin and eosin and Masson trichrome.
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bioRxiv - Neuroscience 2022Quote: ... FoxP2 (mouse, Merck Millipore: MABE415, RRID: AB_2721039; 1:2000; Invitrogen: MA5-31419, RRID: AB_2787055; 1:2000; rabbit, Abcam: ab16046, RRID: AB_2107107; 1:500), Calb1 (rabbit ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies: mouse anti-HSP70 (ab2787, Abcam, Cambridge, MA, USA), rabbit anti-TSG101 (14497-1-AP ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min with primary antibodies diluted in blocking solution as follows: mouse anti-Pyruvate dehydrogenase E2/E3 (PDHE2/E3, ab110333, Abcam, 1 μgmL−1); mouse anti-clathrin heavy chain (X22 ...