Labshake search
Citations for abcam :
4951 - 5000 of 5055 citations for Recombinant Mouse Il13ra1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... We used mouse anti-beta-tubulin (E7, Developmental Studies Hybridoma Bank, 1:300 dilution) and rabbit anti-histone H3 (Abcam, 1:500 dilution). Embryos were then washed 4 x 2 h in PBT and incubated overnight at 4°C in PBT supplemented with goat anti-mouse and goat anti-rabbit secondary antibodies coupled to Alexa Fluor 488 and 568 (Invitrogen) ...
-
bioRxiv - Molecular Biology 2020Quote: ... in a Tris-glycine-methanol transfer buffer overnight and detected with the following antibodies: 1:5000 monoclonal mouse anti-Hsp70 [C92F3A-5] (Abcam Cat# ab47455, RRID:AB_881520) (abcam) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... e cells were then incubated with primary antibodies against mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) (1:200)(ab48508, 1:200 dilution; Abcam, Cambridge, United Kingdom) at 4°C overnight ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used and dilution ratios are: mouse monoclonal anti-Acetylated α-Tubulin (1:500, ab24610, clone 6-11B-1, abcam, Cambridge, MA), mouse monoclonal anti-Acetylated α-Tubulin (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... stained and incubated in a humidified chamber with rabbit anti-mouse Collagen I overnight at 4°C (1:100 dilution Abcam Catalog No. ab34710); washed 3 times in TBS-Tween (.05% Tween ...
-
bioRxiv - Neuroscience 2022Quote: ... for 1 h at room temperature and were incubated overnight at 4 °C with primary rabbit polyclonal antibodies against mouse MAGL (Abcam, Cambridge, UK; ab180016) or human MAGL (LSBio ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were washed and incubated with the appropriate fluorescein-conjugated secondary antibodies (Alexa Fluor 594-labeled goat anti-mouse IgG from Abcam for γ-H2AX; Alexa Fluor 488-labeled goat anti-rabbit IgG from Abcam for RAD51). After washing ...
-
bioRxiv - Immunology 2019Quote: ... The tissue was embedded in paraffin and sections were stained with an antibody against RELM-α (polyclonal rabbit anti-mouse, Abcam, Cambridge, UK). Nuclei were stained with haematoxylin (Merck) ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113; 1:1000 Goat-α-rabbit AlexaFluor555, abcam ab150090) and washed again in PBS (4×10 min) ...
-
bioRxiv - Developmental Biology 2020Quote: ... cover slips were incubated in primary antibody at 4°C overnight followed by rinses in PBS and one hour incubation with goat anti-mouse IgG (Alexa Fluor 647-conjugated) secondary antibody (Abcam, Cambridge, MA, ab150115) and/or goat anti-rabbit IgG (Alexa Fluor 594-conjugated ...
-
bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-HA antibody (Covance, Burlington, NC: H11) and rabbit anti-GFP antibody (Abcam, Cambridge, UK: ab290) were used at 1:500 ...
-
bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 9 was performed using citrate buffer and sections were incubated with the primary antibody rabbit anti‐mouse CD31 (1:50) (ab28364, Abcam, Cambridge, MA, USA) at 4°C overnight ...
-
bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 6 with citrate buffer and the primary antibody rabbit anti-mouse CD11b (1:200) (ab133357, Abcam, Cambridge, MA, USA) and antigen retrieval with 1:20 proteinase K/TE buffer and rat anti-mouse F4/80 (1:100 ...
-
bioRxiv - Plant Biology 2019Quote: ... For the localization of total fraction of RNAPII we used mouse 8WG16 antibody (anti-RNA polymerase II CTD repeat YSPTSPS, Abcam, diluted 1:200), according to protocols provided by Kołowerzo-Lubnau et al64 ...
-
bioRxiv - Biochemistry 2020Quote: ... blots were incubated overnight at 4°C with a mouse antibody against the α subunit of Na+/K+-ATPase (Abcam-ab7671, 1:2,000) and with a mouse antibody against the His-tag (Tetra·His Antibody ...
-
bioRxiv - Cell Biology 2020Quote: ... with fibronectin-depleted (depleted via column purification using gelatin sepharose) FBS at 10% on glass coverslips coated with 10ug/mL murine fibronectin (native mouse fibronectin, Abcam, Cambridge MA, USA) or laminin (laminin mouse protein ...
-
bioRxiv - Cell Biology 2019Quote: ... Rabbit polyclonal HPA antibodies targeting the proteins of interest were dissolved to 2-4 μg/ml in blocking buffer (PBS + 4% FBS) containing 1 μg/ml mouse anti-tubulin (Abcam, ab7291, Cambridge, UK). After washing with PBS ...
-
bioRxiv - Physiology 2019Quote: ... for 1 h at room temperature and next incubated with primary antibodies (p62, CoxIV: Cell Signaling,3E11, Tomm20:Abcam, UK, ab56783 (anti-mouse) or ab186734 (anti-rabbit) ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were then probed with Alexa Fluor anti-rabbit A680 secondary antibody and Alexa Fluor anti-mouse A790 secondary antibody (Abcam ab175773 and ab175783). Membranes were visualized using an Odyssey Infrared Imaging System (LI-COR) ...
-
bioRxiv - Biochemistry 2021Quote: ... the chambered coverglass plates were rinsed three times with PBS and cells were labeled with fluorescently conjugated secondary antibody (anti-Rabbit Alexa Fluor 488, lnvitrogen #A-11008, 1:500; anti-Mouse Alexa Fluor 647, Abcam# ab150119, 1:500) in blocking buffer for one hour at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... then incubated in 5% milk in TBS-T containing 1:10000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) for 1 hour at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... Western blots were imaged on an Odyssey imaging system (Licor) with anti-PAR primary and goat anti-mouse IR680 (Millipore Sigma AM80 and Abcam ab216776, respectively). A dT50 oligo was radioactively labeled with 32P by T4 PNK (NEB M0201) ...
-
bioRxiv - Cancer Biology 2021Quote: ... DNA fibers were incubated with mouse-FITC-anti-bromodeoxyuridine (detects IdU, B44, Becton Dickinson, 1:50) and rat-anti-bromodeoxyuridine (detects CldU, abcam ab6326, 1:100), at 37°C for 1 hour and then with anti-mouse IgG AlexaFluor 488 (Invitrogen A11029 ...
-
bioRxiv - Microbiology 2020Quote: ... the fixed cells were labeled with primary antibodies including goat anti-major outer-membrane protein (MOMP; Meridian, Memphis, TN) and mouse or rabbit anti-six histidine tag (Genscript, Nanjing, China and Abcam, Cambridge, UK, respectively). For experiments using CRISPR interference ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were incubated with matching secondary antibodies (anti-rabbit and anti-guinea pig were the same ones as previously used as well as an Alexa Fluor goat anti-mouse 555 [Abcam, product code: ab150114]) and a DAPI stain was added ...
-
bioRxiv - Cell Biology 2022Quote: ... The fluorescent secondary antibodies (goat anti mouse IgM Alexa Fluor® 647, abcam, ab150123, or donkey anti rabbit IgG Alexa Fluor® 568, abcam, ab175470) and DAPI (SouthernBiotech ...
-
bioRxiv - Plant Biology 2022Quote: ... Shanghai, CN), Goat Anti-Mouse IgG HRP (Abmart, Shanghai, CN) and Anti-Myc antibody (HRP Anti-Myc tag antibody, Abcam, Cambridge, MA, USA).
-
bioRxiv - Physiology 2022Quote: All protein homogenates from human and mouse lungs or HPAECs were subjected to Western blot analysis as reported.23 Primary antibodies were purchased from Abcam (Waltham, MA, USA) and included USP11 anti-rabbit polyclonal antibody (1:250 dilution ...
-
bioRxiv - Microbiology 2022Quote: ... LTA was detected with an anti-LTA primary antibody (mAb 55; HycultBiotech; 1:5000 dilution) and an HRP-goat anti-mouse IgG secondary antibody (Abcam; 1:10000 dilution). LTA was visualised using Amersham ECL Prime western blotting detection reagent (GE Healthcare ...
-
bioRxiv - Molecular Biology 2022Quote: ... SUMO (two antibodies used; mouse anti-SUMO-1, clone 21C7, Zymed, #33-2400, 1:200; rabbit anti-SUMO-1, Abcam, ab32058, 1:200), rabbit anti-DAXX (polyclonal D7810 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Coverslips were incubated with the following primary and secondary antibodies in PBS/10 % FBS for 1 hour to overnight: mouse anti-MRE11 (abcam, #ab214, 1:500), rabbit anti-TFAM (antibodies-online ...
-
bioRxiv - Molecular Biology 2024Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
-
bioRxiv - Microbiology 2024Quote: ... The equivalent of 0.3 OD units was analyzed by SDS-PAGE and western blot using the following antibodies and dilutions: mouse anti-AD (Abcam, cat nr ab135398) 1:200 in 3% BSA in PBS-0.5% Tween (PBS-T) ...
-
bioRxiv - Developmental Biology 2023Quote: ... was used as a positive control to confirm a successful experiment and a secondary antibody anti-Mouse IgG (Abcam, ab46540, 1:50 dilution) was used as negative control to remove background in the following sequencing analysis.
-
bioRxiv - Microbiology 2023Quote: ... Midguts were washed five times via direct tube transfer in PBS+T before staining with the secondary antibody (Goat anti-mouse Alexa Fluor 594, Abcam, Cambridge, United Kingdom) at a 1:1000 dilution in blocking solution for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
-
bioRxiv - Pathology 2023Quote: ... Sections were incubated at room temperature for 1 hour with mouse monoclonal primary antibodies against rat substance P (1:300 dilution, ab14184, Abcam, Cambridge, MA, USA) or normal mouse serum (ab7486 ...
-
bioRxiv - Cell Biology 2023Quote: ... or anti-Sc35 (MilliporeSigma, S4045, 1:1000) and incubated with the secondary antibodies using Alexa 488-labeled goat anti-mouse IgG (Abcam, Ab150113, 1:1000), Alexa 594-labeled goat anti-rabbit IgG (ThermoFisher ...
-
bioRxiv - Immunology 2023Quote: ... in PBS-Tween (1:250, overnight, 4°C) and FITC anti-mouse alpha muscle actin (Abcam, ab8211-500, 1:100, overnight, 4°C). Tissues were washed with PBS-Tween before incubation with secondary antibody PE-Goat anti-Rabbit IgG (Abcam ...
-
bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were blocked in 1% BSA in PBS for 30 minutes at room temperature before overnight incubation at 4°C in primary antibodies: α-mouse CENP-A (1:1000 Abcam ab13939 (discontinued) or 1:1500 Enzo ADI-KAM-CC006-E ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were mounted on slides and stained using a monoclonal mouse anti-muscarinic acetylcholine receptor M4/CHRM4 primary antibody (1:500, abcam, ab77956, RRID: AB_1566454), with blocking reagents and secondary anti-mouse biotinylated antibody from the M.O.M ...
-
bioRxiv - Systems Biology 2023Quote: ... the cells underwent an additional 30-minute blocking step using 1× blocking buffer and were then incubated for 1 hour with secondary antibodies – Goat Anti-Mouse IgG H&L (Alexa Fluor® 594, Abcam, Cambridge, UK), and Phalloidin-iFluor 488 Reagent (Abcam ...
-
bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... samples were incubated with a secondary antibody diluted in 1%BSA PBS-0.05%Tween 20 (goat anti-mouse IgG H&L – Alexa fluor 488 preabsorbed, Abcam, cat# ab150081; 1:500) for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
-
bioRxiv - Genetics 2023Quote: ... The following primary antibodies were used at the indicated dilutions: mouse α-H3K4me3 (1:500, MAB Institute Inc Wako 305-348191) and rabbit α-H3K9ac (1:1000, Abcam ab10812). The secondary antibodies from Jackson ImmunoResearch Laboratories (West Grove ...
-
bioRxiv - Biochemistry 2023Quote: ... The cell lysates were boiled in SDS-PAGE loading buffer for 10 min and then subjected to SDS-PAGE and probed with the following specific antibodies: mouse anti-PrP monoclonal antibody 8H4 (Abcam ab61409, 1:5,000), mouse anti-MyHC antibody MF-20 (Developmental Studies Hybridoma Bank MAB4470-SP ...
-
bioRxiv - Neuroscience 2023Quote: ... using V-PLEX Pro-inflammatory Panel 1 (Meso Scale Discovery, catalogue no. K15048D1) and mouse apolipoprotein E ELISA kit (abcam, catalogue no. ab215086) according to the manufacturers’ instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...