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Citations for abcam :
401 - 450 of 1410 citations for Recombinant Sortase Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... the cells were washed 2x with PBS followed by staining for surelight® APC conjugated anti-his antibody (Abcam, ab72579) for 30 min on ice ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant protein expression was verified by concentration determination using the Bradford method and by Western Blot analysis using the anti-His antibody (dilution 1:1000, Abcam).
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... The plates were washed four times with PBST and were then incubated with 1:15,000 rabbit polyclonal 6x His-tag antibody conjugated to HRP (Abcam, Austria) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The samples along with the control were then loaded on 10% SDS-PAGE followed by immunoblot with anti-6x His or anti-Flag antibody (Abcam).
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-beta IV tubulin recombinant antibody conjugated with Alexa Fluor® 647 [EPR16775] (Abcam, cat# ab204034) was used at a 1:100 dilution.
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bioRxiv - Neuroscience 2022Quote: ... Recombinant Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (abcam, ab209538, 1:200). Lists of secondary antibodies used ...
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bioRxiv - Developmental Biology 2022Quote: ... Organoids recovered from cell seeding were treated with 10 ng/ml recombinant rabbit integrin α2 (AbCam, ab181548) and 10 ng/ml mouse IgG1 integrin β1 (AbCam ...
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bioRxiv - Genetics 2023Quote: ... Then incubated at 4°C overnight with recombinant anti-NADPH oxidase 4 antibody (1:2000, ab133303, abcam), and mouse anti-GAPDH monoclonal antibody was used for normalization (1:3000 ...
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bioRxiv - Genetics 2023Quote: ... according to manufacturer’s instructions using a recombinant rat monoclonal STAG1 antibody (Abcam ab241544, Lot:GR3334172-1; 1:200) after cooking 10 minutes at 110°C in antigen unmasking solution (Vector #H3301) ...
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bioRxiv - Genetics 2023Quote: ... The membrane was then incubated with appropriate primary antibodies: rabbit recombinant anti-MECP2 (ab253197, Abcam, 1:3000), rabbit monoclonal D51G7 anti-IRAK1 (#4504 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4° C overnight in 1:500 recombinant rabbit anti-HA tag (Abcam, AB236632) or rabbit anti-c-Fos (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant Anti-α-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (abcam, ab209538, 1:200). Lists of secondary antibodies used ...
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bioRxiv - Bioengineering 2024Quote: ... recombinant rabbit monoclonal primary antibody against TNF Receptor 2 [EPR1653] (dilution 1:200, ab109322, Abcam, Cambridge, MA) or normal rabbit serum (ab7487 ...
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bioRxiv - Immunology 2022Quote: ... Protein quantification was performed with the BCA protein assay kit (ab207003, Abcam). Proteins were separated by SDS-PAGE and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Oxysterol-binding protein-related protein 8 (anti-OSBP8/ORP8; Abcam, ab99069), anti-Cell cycle control protein 50A (anti-CDC50A/TMEM30A ...
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bioRxiv - Microbiology 2024Quote: ... Proteins on the gels were visualized using InstantBlue Coomassie Protein Stain (Abcam), destaining with distilled water.
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NK cells orchestrate splenic cDC1 migration to potentiate antiviral protective CD8+ T cell responsesbioRxiv - Immunology 2020Quote: ... 1% Protein Block (Abcam), and 2% 2.4G2 supernatant ...
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bioRxiv - Physiology 2020Quote: ... and protein phosphatase (Abcam) inhibitor cocktails ...
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bioRxiv - Physiology 2022Quote: ... CFTR protein (ab2478, Abcam) and Na,K-ATPase α-subunit (ab76020 ...
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bioRxiv - Neuroscience 2023Quote: ... or Protein G (Abcam) beads were added and incubated overnight to preclear the supernatant ...
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bioRxiv - Synthetic Biology 2021Quote: ... the membranes were blocked with 5% milk (w/v) in TBST (TBS, 0.1% Tween 20) and were probed with horseradish peroxidase (HRP) conjugated anti-His antibody (Abcam, catalog # ab1187) or the C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586; anti-N2D, Millipore, MAB5578; anti-strep, Abcam, ab252885; anti-6×his, Abcam, ab15149) and subsequently with secondary antibodies of HRP-conjugated (mouse IgG ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in 20μL of 1X phosphate buffered saline (PBS) and anti-6x His Alexa Fluor 647 (abcam, cat: ab237337) was added at a dilution of 1:100 ...
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bioRxiv - Cancer Biology 2019Quote: ... cultured osteoblasts or osteoclasts were treated with 25 ng/ml recombinant full-length human MMP-3 (Abcam, ab96555) in place of conditioned medium or in combination with conditioned medium.
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bioRxiv - Neuroscience 2020Quote: ... recombinant anti-M6PR (cation independent) antibody (ab124767) and rabbit polyclonal antibody VDAC1 were also purchased from Abcam (ab15895). The mouse monoclonal antibody against total LRRK2 (C-terminus ...
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bioRxiv - Synthetic Biology 2021Quote: ... Recombinant gene product was either detected with Goat anti-GFP conjugated to Alkaline Phosphatase (ab6661 Abcam, Cambridge, Massachusetts) at 1:5000 dilution or 1:5000 Rabbit Polyclonal anti-SARS-CoV2 RBD (Cat# 40592-T62 Sino Biological ...
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bioRxiv - Developmental Biology 2019Quote: ... and the other was embedded into type Ia collagen mixed with either 0.1 mg/ml laminin 211 (Biolamina, human recombinant laminin LN211) or 0.1 mg/ml collagen IV (Abcam, Natural Human Collagen IV protein (FAM ...
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bioRxiv - Immunology 2023Quote: ... and the recombinant Clec4F was detected using a goat anti-human IgG coupled to peroxidase (AbCam, Cambridge, UK). For competition ELISA ...
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bioRxiv - Genomics 2023Quote: ... Antibodies used included 1:1,000 recombinant anti-ERG antibody (ab133264) and 1:5,000 anti-histone H3 antibody (ab1791) (Abcam).
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bioRxiv - Molecular Biology 2024Quote: ... Beads were resuspended in diluted kinase assay buffer containing 0.5 mM ATP and recombinant Aurora B kinase (AbCam). Kinase reactions were shaken at 30 °C for 10 mins at 1000 RPM and quenched with 4x LDS buffer (-EDTA ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were probed with the appropriate primary antibody: Recombinant Anti-Alpha-synuclein antibody [MJFR1] (1:2000 Abcam, ab138501) or β-Actin (1:5000 Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein A sepharose beads (ab193256) and protein G sepharose beads (ab193259) were from abcam.
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bioRxiv - Molecular Biology 2020Quote: ... Plasma membrane proteins were extracted using the Plasma Membrane Protein Extraction Kit from Abcam. Proteins were separated from membrane and cytoplasmic extracts on 8% SDS-PAGE gels and then western blotted with antibodies against PKP2 (EB10841 ...
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bioRxiv - Immunology 2021Quote: ... S1 Spike protein was detected using a Covid-19 S-protein ELISA kit (Abcam) specific to S1RBD ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma membrane proteins were extracted using a plasma membrane protein extraction kit (Abcam, #ab65400), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Plasma membrane proteins were extracted using a plasma membrane protein extraction kit (Abcam, #ab65400), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Protein carbonyl assays used the Oxidized Protein Western Blot Detection Kit (ab178020; Abcam, USA) and were performed as we previously described.36 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and NICD: Mdm2 (MDM2 protein Gln119-Leu438 Origene TP761916; MDM2 protein-full length Abcam, ab82080 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins on the gel were visualized by staining with InstantBlue Coomassie protein stain (Abcam).
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bioRxiv - Developmental Biology 2023Quote: ... Protein concentrations were determined using the BCA Protein Quantification Kit (Abcam, Cambridge, UK, ab102536) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Both the supernatant and pellet (after washing with buffer F) were resuspended with SDS-loading buffer for SDS-PAGE and western blotting probed by mouse anti-His antibody (Abcam, MA, USA).
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bioRxiv - Immunology 2023Quote: ... H3Cit staining of fixed surface labelled cells was performed by incubating the cells overnight at 4°C in PBS 2% HI-FBS + 2mM EDTA + 0.1% saponin with monoclonal anti-H3Cit antibody (Abcam, ab219407, 1:600). Cells were washed and then incubated with donkey anti-rabbit AF555 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773, Goat pAb to rabbit IgG)) at a 1:5,000 dilution to detect RhaPS and His-tagged carrier proteins ...
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bioRxiv - Bioengineering 2020Quote: ... Collagen IV protein (Abcam, ab7536) and Matrigel (growth-factor reduced ...
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bioRxiv - Physiology 2019Quote: ... total OXPHOS protein (Abcam, #ab110413), mt-Co2 (Proteintech ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated Protein-G (abcam), and Alexa Fluor 488/555-conjugated anti-goat/mouse IgG donkey polyclonal antibodies (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein blocking solution (Abcam). They were then incubated overnight at 4°C with primary antibody (Mouse TIF-IA (Abcam) ...
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bioRxiv - Immunology 2023Quote: ... fibroblast activation protein (FAP, Abcam rabbit monoclonal antibody EPR20021 ...
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bioRxiv - Molecular Biology 2023Quote: ... green fluorescent protein (ab290, Abcam) Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... followed by protein block (Abcam) for 30 minutes and were then incubated with relevant primary antibodies diluted in antibody diluent (Abcam ...