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Citations for abcam :
351 - 400 of 1410 citations for Recombinant Sortase Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... recombinant anti-Tumour susceptibility gene 101 (TSG101) (1:1000, Rabbit monoclonal (Abcam, Melbourne, Australia); anti-CD9 (1:1000 ...
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bioRxiv - Physiology 2024Quote: ... the recombinant phospho-Thr73-Rab109 (#ab230261) and Rab10 total (#ab237703) were purchased from Abcam. The total Rab10 mouse antibody was from NanoTools (0680–100/ Rab10-605B11) ...
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bioRxiv - Cancer Biology 2023Quote: ... Complete medium supplemented or not with recombinant IL-1β (25ng/ml) (Abcam, cat.no. ab259421) was used as a positive and negative control ...
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bioRxiv - Molecular Biology 2024Quote: ... and Recombinant Alexa Fluor 647 anti-Cytokeratin 5 antibody diluted 1:200 (Abcam, ab193895) and DAPI (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and recombinant Alexa Fluor® 647 Anti-Integrin beta 1 antibody (EPR16895) (abcam, 225270) at 1:100 dilution ...
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bioRxiv - Genetics 2024Quote: ... recombinant rabbit anti-Sodium Potassium (Na+/K+) ATPase [EP1845Y] (1:4000, Abcam Cat. # ab76020), rabbit anti-Lasu1/Ureb1 (HUWE1 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then incubated with recombinant anti-CDKN2A/ p16Ink4a antibody (1:100, ab211542, Abcam) in blocking buffer overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... We used primary antibodies included recombinant anti-LDL receptor antibody (diluted 1:500, Abcam), anti-LRP1 antibody produced in rabbit (diluted 1:1000 ...
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bioRxiv - Synthetic Biology 2021Quote: A clonal line of K562 cells expressing a doxycycline-inducible FLAG-tagged ALPPL2 cassette was treated with doxycycline (Abcam) at doses ranging between 0.1-100 ng/mL for 24 hours prior to co-incubation with T cells ...
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bioRxiv - Neuroscience 2019Quote: ... sections with rabbit primary antibody were incubated with 1:500 goat-anti-rabbit Cy3-tagged secondary antibody (Abcam, USA) for one hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged ribosomes were collected by indirect IP by adding 40 ug of anti-HA antibody (Abcam ab9110, RRID:AB_307019) to CB lysate pools and 5 ug to NP lysate pools ...
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bioRxiv - Synthetic Biology 2021Quote: The primary antibodies were tagged successively with goat anti-rabbit antibodies conjugated with horseradish peroxidase (HRP) (Abcam, catalog # ab6721). The bound antibodies were detected using luminol containing reagent Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Immunology 2023Quote: ... Proteins were stained using InstantBlue Coomassie protein stain (Abcam). Molecular weights were manually estimated according to the Novex Sharp protein marker ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP-KIF2A and a FLAG-tagged component were overexpressed and the following antibodies were used: rabbit anti-GFP (Abcam, ab290), mouse anti-FLAG M2 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were then washed thrice with TBS-T and probed with secondary goat anti-mouse and anti-rabbit tagged with HRP (Abcam). The secondary antibodies were diluted in blocking solution and it was a 1/2000 dilution factor ...
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bioRxiv - Molecular Biology 2021Quote: ... Further primary antibodies used were recombinant anti-calbindin antibody (1 μg mL-1, ab108404, Abcam), anti-glial fibrillary acidic protein (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were stained using the Recombinant Anti-Histone H4 (tri methyl K20) antibody (Abcam, ab177190) at 1:4000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... Peptide conjugated resin was incubated with a mixture of 11 recombinant PTP (abcam, Cambridge, UK) (each 1.5 pmol ...
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bioRxiv - Genomics 2022Quote: ... and stained with primary recombinant Alexa Fluor® Anti-FKBP51 antibody (ab198979, Abcam, 1:100) diluted in 1% BSA-PBS or left unstained ...
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bioRxiv - Bioengineering 2023Quote: ... at a dilution of 1:200 and recombinant rabbit monoclonal anti-vinculin 35 (abcam, ab129002) at a dilution of 1:100 ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with a recombinant rabbit anti-mouse CD4 monoclonal antibody (clone EPR19514; Abcam) diluted 1:4,000 in a ready-to-use antibody diluent (Leica Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were used as follows: Rabbit recombinant monoclonal anti-somatostatin (1:250, Abcam, ab111912), mouse monoclonal anti-ChAT (1:250 ...
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bioRxiv - Cell Biology 2020Quote: ... The following primary antibodies His-Tag (1:200, Affinity, T0009, USA) and ACE2 (1:200, Abcam, ab15348, UK) were used ...
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bioRxiv - Cell Biology 2020Quote: ... The following primary antibodies His-Tag (1:200, Affinity, T0009, USA) and ACE2 (1:200, Abcam, ab15348, UK) were used ...
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bioRxiv - Cell Biology 2020Quote: ... Then, anti-His-Tag (1:200, Affinity, T0009, USA) and anti-ACE2 antibodies (1:100, Abcam, ab15348, USA) were added and the cells were incubated overnight at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated overnight at 4°C with a mouse monoclonal anti-His-tag primary antibody (Abcam, ref. ab18184) diluted 1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... the flow cell was incubated with a biotinylated anti-6X His tag antibody (cat #ab27025, Abcam, Cambridge, MA) (1 µg/mL ...
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bioRxiv - Microbiology 2020Quote: ... The following near infrared (NIR) fluorescently tagged secondary antibodies were used: IRDye® 680RD Goat Anti-Rabbit (IgG) secondary antibody (Ab216777, Abcam) and IRDye® 800CW Goat Anti-Mouse (IgG ...
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bioRxiv - Biochemistry 2020Quote: ... bound proteins were resolved by SDS-PAGE and western blots were performed with the anti-FLAG antibody to validate the immunoprecipitation of FLAG-tagged fl-SNED1 and with anti-phosphoserine (1 μg/mL; Abcam, ab9332), anti-phosphothreonine (1 μg/mL ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.1% Tween 20) and then probed with horseradish peroxidase (HRP) conjugated anti-His antibody (1: 5,000) (Abcam, catalog # ab1187) or rabbit polyclonal serum ...
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bioRxiv - Bioengineering 2020Quote: Tissue levels of MMP12 in Hi-Myc and healthy FVB/NJ prostate homogenates were measured by ELISA according to manufacturer’s protocol (ab246540, abcam). Prostate homogenates were prepared as previously described ...
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bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-trypsin antibody (Ab-200997) and mouse anti-his tag (Ab-49936) were obtained from Abcam (Cambridge, UK). Affi-gel 10 resin was obtained from Bio-Rad laboratories (Hercules ...
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bioRxiv - Microbiology 2020Quote: ... In a first step the membranes were incubated either with 0.1 μg/mL Anti-6×His® antibody (Abcam) to detect EF-P ...
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bioRxiv - Biophysics 2024Quote: ... Successful overexpression of AGO2 was confirmed using Western Blot analysis with an anti-6X His tag antibody (Abcam, AB18184).
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bioRxiv - Microbiology 2023Quote: ... The blot was probed with an anti-His-tag antibody conjugated to horseradish peroxidase (HRP) (Abcam, Cat No. ab1178). Alternatively ...
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bioRxiv - Microbiology 2024Quote: ... 0.05 % Tween20) and then incubated with horseradish peroxidase (HRP) conjugated anti-His antibody (1:10,000 dilution; Abcam, United Kingdom) in TBST with 5 % (w/v ...
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bioRxiv - Immunology 2022Quote: Recombinant human peroxiredoxins 1 and 6 (PRX1, PRX6) were purchased from Abcam (Abcam PLC, Cambridge, UK), while recombinant human β-defensin-1 and 2 (HBD-1 ...
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bioRxiv - Bioengineering 2020Quote: Human recombinant αSyn monomers and pre-formed fibrils were purchased from Abcam (Monomers; ab218819, Fibrils; ab218819), and were diluted in DopaNeurons Media to a final concentration of 4 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: We used the following antibodies: Recombinant monoclonal anti-Thiophosphate ester rabbit IgG [51-8] (Abcam, ab92570), monoclonal anti-GFP mouse IgG (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... were coated with 1% BSA or 20 nM of GST-fused recombinant human CCR5 (hrCCR5, Abcam) in PBS overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... incubated with primary antibodies for Paxillin (Recombinant Anti-Paxillin antibody [Y113], Abcam ab32084; 1:250 dilution) and Myosin (Phospho-Myosin Light Chain 2 (Ser19 ...
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bioRxiv - Microbiology 2022Quote: ... cells were dissociated and incubated with recombinant human ACE2-Fc(IgG) (1 mg/ml) (Abcam ab273687) at a dilution of 1:32000 for 30 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Neuropeptide antibodies raised in mouse were combined with rabbit recombinant anti-alpha tubulin antibody (abcam, ab190471). All primary antibodies were diluted 1:100 in blocking solution ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained overnight at 4oC using Recombinant Anti-GC1q R antibody [EPR23238-6] (Abcam ab270033).
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bioRxiv - Cell Biology 2021Quote: ... Acetylated Histone 4K16 was detected using a recombinant rabbit antibody (Abcam, ab177191, GR284778-8, 1:400) and visualized using a donkey anti-rabbit Alexa Fluor 488 secondary antibody (Molecular Probes ...
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bioRxiv - Developmental Biology 2023Quote: 1 µg purified recombinant human PARD3 was incubated with 750ng of GST-CDK1/CyclinB1 (abcam; ab271456) in kinase buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then incubated with either recombinant anti-CDKN2A/ p16Ink4a antibody (1:100; Abcam, catalogue #ab211542), sheep anti-calcitonin gene-related peptide (CGRP ...
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bioRxiv - Physiology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Reagent (Abcam, USA). Proteins were separated on SDS-PAGE and transferred to the polyvinylidene fluoride (PVDF ...
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bioRxiv - Synthetic Biology 2021Quote: ... for Im7 variants or 50 μL of 1:5,000-diluted HRP-conjugated anti-6×His tag antibody (Abcam, catalog # ab1187) for scFv-HER2 variants ...
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bioRxiv - Pathology 2022Quote: ... The pull-down reactions were analyzed by SDS-PAGE followed by Western Blot using anti-His (M20001; Abmart) and anti-GST (ab111947; Abcam) antibodies.