Labshake search
Citations for abcam :
4251 - 4295 of 4295 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: TNF-α concentrations in undiluted or appropriately diluted supernatants were determined by ELISA using the SimpleStep human TNF alpha ELISA kit (abcam, Cambridge, MA), performed following the manufacturer’s instructions and with endpoint absorbance measurements at 450 nm on an Infinite M200 Pro plate reader (Tecan ...
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bioRxiv - Neuroscience 2020Quote: Absolute quantification of sialic acid in different glycoconjugate fractions was performed using the Sialic Acid (NANA) Assay Kit (Ab83375, Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... equal number of cells were lysed using assay buffer provided in the alpha KG assay kit (abcam, ab83431, lot no. GR3410863-1). The lysates were then deproteinized using perchloric acid and nutralized using 2M KOH as per manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Cell cycle analysis was performed by propidium iodide (PI) staining following the protocols from the propidium iodide flow cytometry kit (Abcam, Cat. ab139418). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... None of the cultures or treatments showed signs of cell cytotoxicity using the using the LDH-Cytotoxicity Assay Kit II (Abcam, cat. #ab65393) to analyze 50 ul fresh culture media (not shown) ...
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bioRxiv - Physiology 2024Quote: Mitochondrial membrane potential was measured in γY4KD cells with Pparγ silenced with siRNA and their scrambled control (Scrl) using Mitochondrial Membrane Potential Assay kit (Abcam, Cat# ab113852). Cells were plated on collagen coated 24-well plate at density of 50,000 cells/well (5 well/per group) ...
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bioRxiv - Neuroscience 2024Quote: Choline and acetylcholine were quantified in rat cortical tissue from all experimental groups using a choline/acetylcholine assay kit (ab65345, Abcam, Cambrige, UK). 10 mg of cortical fresh tissue were harvested ...
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bioRxiv - Bioengineering 2024Quote: ... were used to quantify the ratio of reduced (GSH) to oxidized (GSSG) circulating glutathione with a commercial ELISA kit (Abcam; Cambridge, UK) as an indicator of oxidative stress levels and redox imbalance.33
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bioRxiv - Neuroscience 2023Quote: ... serotonin and brain derived neurotrophic factor using commercially available Enzyme linked immunosorbent assay kits according to the instructions of the manufacturer (ABCAM, Cambridge UK)
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bioRxiv - Genomics 2023Quote: The cholesterol assay was carried out according to the manufacturer protocol using the Cholesterol/Cholesterol Ester Assay Kit (ab65359, Abcam, Cambridge, UK) and normalized to total protein content as determined by Bradford Assay.
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bioRxiv - Biochemistry 2023Quote: Right Ventricular Mitochondrial Enrichments: RV mitochondrial enrichments were isolated from the same PAB and control piglets with a mitochondrial isolation kit (Abcam, Cambridge, MA) as previously described(10 ...
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bioRxiv - Pathology 2023Quote: ... regularly spaced (500 µm) along the rostrocaudal axis were stained with a Prussian blue and nuclear fast red stain kit (Abcam, Cambridge, UK) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: RCC243 cells were treated with 5 μM MS023 for the indicated time periods and then cells were trypsinized and stained using the Propidium Iodide Flow Cytometry Kit (Abcam, #ab139418, Abcam) based on the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Cancer Biology 2024Quote: ... the adherent monolayers were incubated with 100 µl/well cell lysis buffer from the cAMP Direct Immunoassays Kit (Fluorometric, ab138880, Abcam, Cambridge, UK), while the gliomaspheres were collected ...
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bioRxiv - Pathology 2024Quote: ... Masson’s trichrome staining was performed to assess endometrial fibrosis in sections within a 20 μm distance of the FOXA2-assayed sections using a kit (ab150686, Abcam; Waltham, MA) according to manufacturer instructions.
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bioRxiv - Neuroscience 2024Quote: ... The activity of cytosolic- or secretory-phospholipase-A2 was measured according to the procedures described in the kit manuals (Abcam, Cambridge, UK).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the sections were incubated with histofine simple stain kit Rat MAX-PO(G) (414331F, Nichirei, Tokyo, Japan) or goat anti-chicken IgY conjugated biotin (ab6876, abcam plc, Cambridge, UK) for 30 minutrs at RT ...
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bioRxiv - Immunology 2021Quote: ... Free fatty acid concentration in plasma (diluted 1:25) of clamped mice was determined using a quantitative Free Fatty Acid Assay Kit (Abcam, Cambridge, Great Britain). Absorbance was measured with the Filter Max F5 device (Molecular Devices ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were rinsed with phosphate-buffered saline (PBS) and then stained in the culture well using the Adipogenesis Assay Kit (cell-based, Abcam Catalog No. ab133102). The dye was eluted using the kit’s elution buffer and quantified using a microplate reader at 490 nm wavelength ...
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bioRxiv - Physiology 2020Quote: ... and IL-10 concentrations were measured using ELISA kits (TNF-α: R&D Systems, Minneapolis, MN, USA; IL-1β, IL-6, and IL-10: Abcam), in accordance with the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Triton X-100 in 1X PBS for 20 min and then endogenous biotin was blocked using Avidin/Biotin blocking kit (Abcam; cat. no. ab64212) to reduce non-specific background ...
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bioRxiv - Cell Biology 2024Quote: The amount of collagen in liver tissues was determined by measuring the content of hydroxyproline using a Hydroxyproline assay kit (Abcam, Cat#MAK008-1KT) as described by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... and the quantification of intracellular and extracellular total cholesterol and its fractions was measured using the Cholesterol Assay Kit (Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The main pro-inflammatory and anti-inflammatory cytokines in blood and heart were measured by corresponding ELIZA kit: tumor necrosis factor-ɑ (TNF-ɑ, Abcam, ab100747), interleukin-1β (IL-1β ...
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bioRxiv - Cell Biology 2023Quote: The chymotrypsin-like activity of the 20S proteasome was assessed using a commercially available fluorometric proteasome activity assay kit (Abcam, catalog number: ab107921) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: Chromatin accessibility in isolated cardiac fibroblasts or whole heart from C57BL/6J mice was measured using the Chromatin Accessibility Assay Kit (Abcam, cat. no. ab185901) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Unless specified below, all reagents were from MilliporeSigma (St. Louis, MO, USA). LDH assay kit (catalog no. ab65393) was from Abcam (Cambridge, MA, USA). P ...
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bioRxiv - Neuroscience 2023Quote: ... using V-PLEX Pro-inflammatory Panel 1 (Meso Scale Discovery, catalogue no. K15048D1) and mouse apolipoprotein E ELISA kit (abcam, catalogue no. ab215086) according to the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: Three 5 µm sections taken at 150 µm intervals were used for analysis from each animal and were stained with hematoxylin and eosin using the reagents provided in Oil Red O Stain Kit (Abcam, Waltham, MA, USA) for hematoxylin and eosin purchased from StatLab (StatLab ...
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bioRxiv - Developmental Biology 2024Quote: Serum vitamin D (25(OH)D) levels of mouse serum samples were assessed by commercially available ELISA kits (Eagle Biosciences, Inc. Amherst NH, or Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Paraffin-embedded slides were also used for the TUNEL assay and processed according to manufacturer instructions (Abcam, TUNEL Assay Kit - HRP-DAB ab206386). The prepared sections were analyzed using a Nikon Eclipse 80i microscope ...
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bioRxiv - Physiology 2024Quote: ... The lyophilized products were dissolved in 50-150 µL sample diluent and the levels of IFN-α or IFN-β were measured with ELISA kits (IFN-α: Cat. # ab213479; Abcam, Amsterdam ...
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bioRxiv - Immunology 2022Quote: ... TSLP and pCol1 in cell culture supernatants were determined using ELISA kits (R&D System for Areg, IL-33 and TSLP, and Abcam for pCol1). Unless stated otherwise ...
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bioRxiv - Immunology 2022Quote: ... Transiently transfected cells were stained the following day with mAbs conjugated using DyLight® 650 Conjugation Kit (Fast) - Lightning-Link (Abcam, catalog no. ab201803) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies were fluorescently labelled with AF647 or AF488 using the Alexa Fluor® Conjugation Kit (Fast) - Lightning-Link (Abcam, Cat. No. ab269823 and ab236553) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The sample fractionation by protein size was conducted through an eight-channel GELFrEE® 8100 Fractionation System (GELFrEE® 8100 Fractionation System, 8% Cartridge Kit, #42103, Abcam, Cambridge, UK). The cartridge was prepared according to the manufacturer instructions ...
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bioRxiv - Physiology 2024Quote: ... was measured in plasma and brain homogenate by quantifying the adduct generated when MDA in the sample reacts with thiobarbituric acid (TBA) (Abcam, ab118970, Lipid-Peroxidation Kit).
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bioRxiv - Genomics 2024Quote: ... Hematoxylin and eosin (H&E) and Trichrome staining was performed using standard protocols using and H&E and Trichrome Stain Kits (Abcam, ab245880 and ab150686 respectively). The processed sections were visualized using a Leica DM5500 microscope objective magnifications ...
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bioRxiv - Neuroscience 2020Quote: ... NAD and NADH extractions were performed on 20mg PBS washed fresh tissue on wet ice homogenised with a Dounce homogeniser with 400uL of NADH/NAD extraction buffer (Abcam NAD/NADH Assay kit-ab65348). For details ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated for 1 hour in the antibody incubation buffer (Massive Photonics, Buffer kit) with anti-guinea pig AlexaFluor 405 (Abcam, cat ab175678, 1:1000 dilution), phalloidin AlexFluor 488 (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Anti- NA antibodies (2E01) or anti-HA antibodies (CR8033 and CR8071) were previously biotinylated using biotin conjugation kit (Abcam, Fast, Type A, Lightning-Link®). Plates were washed again in PBS-T (0.05% Tween-20 in PBS ...
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bioRxiv - Microbiology 2020Quote: ... At 24 h post-infection flow cytometry was performed targeting NP: cells were processed with the BD Cytofix/Cytoperm™ Kit (catalog no. 554714) and stained with anti-NP antibody (Abcam, clone 9G8, catalog no. ab43821) followed by donkey anti-Mouse IgG Alexa Fluor 488 (Invitrogen catalog no ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 10 µl of the supernatant obtained after the first centrifugation at 18000g from the Glycogen assay where combined with 90 µl of Lactate assay Buffer (Abcam® L- Lactate Assay kit, #ab65331) and separated in two wells of a 96 well plate for sample and sample background control ...
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bioRxiv - Physiology 2024Quote: ... The lyophilized products were dissolved in 50-150 µL sample diluent and the levels of IFN-α or IFN-β were measured with ELISA kits (IFN-α: Cat. # ab213479; Abcam, Amsterdam, Netherlands; IFN-β: Cat. # ab278127; Abcam, Amsterdam, Netherlands) according to the manufacturer’s instructions.