Labshake search
Citations for abcam :
4151 - 4200 of 4295 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Mitochondrial membrane potential in C2C12 myotubes was measured in a fluorescence plate reader (535 nm/580 nm (ex./em, respectively) with 50 nM TMRE and following the TMRE Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... cellular lysates were incubated for 1h with LND or vehicle with was measured using a hexokinase assay kit (Abcam, ab211103) according to manufacturer instructions.
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bioRxiv - Physiology 2024Quote: ... an aliquot of whole tissue lysate was used for plasma membrane purification using the Plasma Membrane Purification Kit (ab65400, Abcam) as previously described.40 Concentrations of proteins were measured using the Bradford assay and stored at -80°C until western blotting.
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bioRxiv - Cell Biology 2024Quote: Enzymatic activity of secreted LOX was measured in 10 μl cell culture supernatants using the fluorometric Lysyl Oxidase Activity Assay Kit (Abcam). LOX activity kinetics was evaluated by measuring fluorescence (Ex/Em = 535/587 nm ...
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bioRxiv - Genetics 2024Quote: ... purified Lp(a) preparations were treated with a variety of fluorescent labeling strategies – FITC Conjugation Kit (Abcam, Cambridge UK, #ab188285), Dil (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate ...
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bioRxiv - Cancer Biology 2024Quote: Matrix metalloproteinase (MMP) activities (as a general enzyme class) were assayed using abcam MMP Activity Assay Kits (Cat # ab112146, Fluorescent [green]; abcam, 152 Grove Street ...
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bioRxiv - Pathology 2024Quote: ... microsome were isolated from the livers of control (Mboat7fl/fl) or hepatocyte-specific Mboat7 knockout mice (Mboat7HSKO) using microsome isolation kit from Abcam and protein was quantified using the bicinchoninic assay (Pierce) ...
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bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
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bioRxiv - Physiology 2024Quote: We utilized two commercially available enzyme-linked immunosorbent assay (ELISA) kits for the quantitative analysis of IL-6 (Abcam, ab100713) and IL-8 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... mouse PGE2 ELISA Kit (ab287802and Cytokine & Chemokine 36-Plex Mouse ProcartaPlexTM Panel 1A (EPX360-26092-901) for Luminex assay were obtained from Abcam and Thermofisher respectively ...
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bioRxiv - Synthetic Biology 2024Quote: ... the supernatant was diluted 1:25000 or 1:50000 and quantified using an Albumin pig ELISA kit (Abcam, Cambridge, UK) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Plasma GLP-1 concentrations were analyzed by an experimenter blinded to treatment conditions in the DRC Radioimmunoassay and Biomarkers Core at Penn using the Human GLP-1(7-36) ELISA Kit (ab184857; Abcam) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... GDH activities in primary astrocytes were determined with glutamate dehydrogenase activity assay kit following with the manufactureŕs instructions (Abcam, ab102527).
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bioRxiv - Neuroscience 2024Quote: ... and nuclear PPARα in V1 were measured using ELISA kits according to manufacturer’s instructions (total AMPK: ab181422, Abcam, UK; phosphorylated AMPK: KHO0651, ThermoFisher, UK; PPARα: ab133107, Abcam, UK). For tissue collection ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended at 1×105 cells/mL in ice-cold PBS and analysed using the comet assay kit (Abcam, #ab238544) according to manufacturer’s instructions with gel electrophoresis run for 20 minutes at 3 volts/cm.
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bioRxiv - Cell Biology 2019Quote: The TC and free cholesterol content of hLECs were measured colorimetrically using a Cholesterol assay kit according to the manufacturer’s protocol (#ab65359; Abcam, Cambridge, UK). Harvest the amount of hLECs necessary for each assay (initial recommend = 1 x 107 cells) ...
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bioRxiv - Immunology 2021Quote: ... Intracellular HO-1 staining for human and mouse was performed using the Invitrogen FIX & PERM Kit (GAS003) using HO-1 PE at 1:100 dilution (Abcam, ab83214) and PE IgG2b isotype (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... NAD+/NADH ratio and the concentration of total NAD+ and NADH was determined by NAD/NADH fluorometric assay kit from Abcam (ab176723) following manufacture’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Levels of anti-inflammatory cytokine Transforming growth factor-β1 (TGF-β1) in supernatants were measured by an ELISA kit (ab119558, abcam, UK), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Oil Red O (ORO) staining was performed on muscle cryosections (10μm) to visualize neutral lipids using an ORO kit (Abcam, Cambridge, GBR). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... The general activity of MMPs in iPSC-astrocyte supernatants was determined using the MMP Activity Assay Kit (Abcam, Cat. no.: ab112147) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP for RNA polymerase II was performed according the iDeal ChIP-Seq Kit instructions using the anti-Pol2 antibody (Abcam, ab5408). Immunoprecipitated DNA was bisulfite converted using the EZ DNA Methylation Gold Kit (Zymo) ...
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bioRxiv - Microbiology 2020Quote: Quantification of the amount of human ACE2 in the different EV and fractions was done using the human ACE2 ELISA kit (Abcam, ab235649) following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of immunoglobulin A (IgA) and immuno-globulin M (IgM) were measured using enzyme-linked immunosorbent assays kits (ELISA; Abcam, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and virus replication was quantitated by measuring the p24 antigen released in culture supernatants at different time points using HIV-1 p24 SimpleStep ELISA kit® (Abcam). Serial dilutions of HIV-1 p24 protein (strain 92418 ...
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bioRxiv - Molecular Biology 2021Quote: The activity of mitochondria Complex I was measured in peeled murine esophageal epithelium using Complex I Enzyme Activity Assay kit (Abcam, ab109721) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Cathepsin B activity was assessed in mouse primary neuronal samples and mouse brain hippocampus using Fluorometric Cathepsin B activity assay kit (abcam, 65300) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells were washed three times with water and then partially lysed with a yeast nuclei isolation kit (Abcam 206997) using the following modified protocol ...
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bioRxiv - Microbiology 2022Quote: ... solani IPO 2222 WT strain and ΦD5□resistant Tn5 mutants were isolated using a Lipopolysaccharide Extraction Kit (Abcam, Symbios, Gdansk, Poland) with a modified protocol (Bartnik et al. ...
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bioRxiv - Immunology 2022Quote: ... Alanine liberated was measured as increasing fluorescence signal (Resorufin, Ex/Em: 535/587 nm) recorded at 25 °C using an L-alanine assay kit (Abcam, ab83394) at 25 °C according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and CTLA-4 Ig (Abatacept) were generated using the APC Conjugation Kit - Lightning-Link® according to manufacturer’s instructions (Abcam, ab201807). APC conjugated anti-PD-L1 antibodies were procured from Biolegend (clones MIH1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were gently washed in phosphate buffered saline (PBS) prior to blocking with the blocking reagent from the mouse-on-mouse polymer IHC Kit (Abcam ab269452). The tissues were then incubated overnight with the primary antibody at 4°C ...
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bioRxiv - Immunology 2020Quote: Transferrin and cytokines in the samples (cell supernatant or plasma) were measured using a mouse transferrin ELISA kit (ab157724, Abcam, USA), mouse TNF-α ELISA kit (DKW12-2720-096 ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Immunohistochemistry for polymerised α1-antitrypsin was performed using 2C1 monoclonal primary antibody and a Mouse-on-Mouse Polymer kit (Abcam AB127055) for detection ...
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bioRxiv - Cancer Biology 2020Quote: Mammary glands were dissected and homogenized in NOS assay buffer and further processed following the Nitric Oxide Synthase Activity Assay kit (Abcam, ab211083) protocol for measuring enzymatic activity of nitric oxide synthase (NOS) ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear protein sample extract from washed cell pellets was carried out according to the manufactures protocol using cell fraction kit – standard (Abcam, ab109719). Nuclear pellets were resuspended in 100mM ammonium bicarbonate and lysed by sonication at 26 joules ...
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bioRxiv - Cancer Biology 2020Quote: ... IL-6 levels in TCM of met-low and met-high melanoma and breast carcinoma tumors were quantified using a specific enzyme-linked immunosorbent assay (ELISA) kit (ab46100, Abcam, US). The ELISA experiments were performed with 5-8 mice per group ...
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bioRxiv - Neuroscience 2021Quote: ... NT-3 was measured by standard ELISA according to manufacturer’s instructions (ab100615 – Neurotrophin 3 human ELISA Kit, abcam, Cambridge, United Kingdom).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab Products AB, Askim, Sweden) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Aperio CS2 image capture device.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with CM for 24 or 48h after which cells were stained to detect DNA fragmentation in apoptosis using the BrdU-Red DNA (TUNEL) kit (Abcam, ab66110) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Histological analyses of the NAFLD tissues were performed using the H&E Stain Kit (Hematoxylin and Eosin) (ab245880; Abcam, Cambridge, UK) and the Oil Red O Stain Kit (Lipid Stain ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Olympus BX60F microscope with an SC50 camera.
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bioRxiv - Cell Biology 2021Quote: The relative contents of endogenous sphingomyelins in WT and SIRT1 KO mESCs were analyzed using a commercially available Sphingomyelin Assay Kit (Abcam ab133118) per manufacturer’s instruction.
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bioRxiv - Cell Biology 2022Quote: ... Sorted satellite cells were treated with UV radiation at different dosages and the comet assay was operated immediately after irradiation through a Comet Assay Kit (Abcam, ab238544) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Grown BEC-organoids were treated with the FA-mix for 4 days and triglyceride levels were measured with a Triglyceride kit (Abcam, ab65336) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: One hundred µl of the cell culture was transferred into a 96-well microplate provided by the In-Cell ELISA kit (Abcam, ab111541), and treated following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Monoclonal α-GPEET and α-EP IgG were conjugated with Allophycocyanin (APC) fluorescence molecule by APC Conjugation Kit – Lightning Link (Abcam). Cells were stained with α-GPEET or α-EP or both α-GPEET and α-EP to detect the loss of GPEET and EP as the indicator of the presence of non-PCF cells in the induced culture ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The final concentration and integrity of eGFP-WNT-3A in the conditioned medium were determined using both ELISA (GFP ELISA® kit, #ab171581, Abcam) and SDS-PAGE/Western Blot analysis ...
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bioRxiv - Neuroscience 2023Quote: Conditioned media from the somatic side of the same MFCs used for the ELISA was analysed with the Cytotoxicity LDH assay kit (Abcam #ab65393) following manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Immunohistochemical staining for MMP13 was performed utilizing the Innovex Universal Animal IHC Kit and a polyclonal rabbit anti-mouse MMP13 antibody (Abcam ab39012). Briefly ...