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Citations for abcam :
3251 - 3300 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed with TBS-T and incubated for 1 hour at room temperature with a 1:10,000 dilution of HRP-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG) in 5% milk-TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.05% Tween 20 in PBS for 1 hr followed by incubation in primary antibodies ((MYC 1:100, Abcam Cat# 32072, γH2AX 1:100 Abcam Cat# 303656) for overnight at 4°C in a humid chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used were (H3K27me3 Cell Signaling C26B11 1:500, EZH2 Cell Signaling 5246S 1:200, B2M Millipore MABF1968 1:2000, HLA-DR,DQ,DP AbCAM ab7856 1:500 ...
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bioRxiv - Genomics 2023Quote: ... cells were blocked for 1 h in 1×PBS + 2% w/v BSA and then incubated with mouse anti-E-cadherin (1:1000) (Abcam, cat#231303) and chicken anti-vimentin (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... Cryosections were blocked with 1% bovine serum albumin in PBS for 1 h at room temperature and incubated with primary antibodies (anti-perilipin 1, PA5-72921, 1:200, ThermoFisher, Waltham, MA, USA; anti-laminin, ab11575, 1:200, Abcam, Cambridge, UK) diluted in 1% BSA in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed for the HLA class I heavy chain (HCA2, 1:1000, Nordic Bio, MUB2036P; HC10, 1:3200, Nordic Bio, MUB2037P) and β-2m (EPR21752-214, 1:4000, Abcam, ab218230) on all generated TMAs as described previously42 ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked in 5% normal serum in 1× PBS Triton 0.3% for 1 hr at room temperature and then incubated with the primary antibody (anti-S100b diluted 1:1000, Abcam, ab5264 or anti-RPE65 diluted 1:250, Thermo Fisher Scientific, MA1-16578; anti-CD34 diluted 1:200, abcam, ab8158) diluted in blocking solution for 1.5 hours at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Resin was washed 4X in 800 uL EMB 300 Buffer and then protein association was analyzed by Western blot using antibody for hnRNP A2/B1 (Abcam #ab6102). Additionally ...
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bioRxiv - Neuroscience 2021Quote: ... Our antibody against DARPP-32 binds to a 16 AA segment on a region of the protein that does not contain any phosphorylation sites (Abcam ab40801) and therefore it labels DARPP-32 irrespective of phosphorylation state and cellular location ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated overnight at 4 °C with Protein G magnetic beads (Dynabeads: ThemoFisher) that had been pre-incubated with anti-H3K27Ac (Abcam, ab4729) or anti-Sox2 (R&D ...
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bioRxiv - Systems Biology 2020Quote: ... The blot was probed with the antibody against the viral nucleocapsid protein (Cambridgebio, USA) and the horseradish peroxidase-conjugated Goat Anti-Rabbit IgG (Abcam, USA) as the primary and the secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the PNBM treated cell lysates were analyzed with a western blot assay to measure protein thio-phosphorylation with the anti-thiophosphate ester rabbit monoclonal antibodies (Abcam, USA).
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bioRxiv - Immunology 2021Quote: ... Staining was completed per manufacturer’s instructions for RNAScope® multiplex V2 detection kit (ACD Bio) coupled with immunofluorescent protein staining for either Cytokeratin19 (ab52625 Abcam) at 1:250 dilution ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The monoclonal anti-VGLUT2 antibody was raised in Mice immunized to a full length recombinant protein corresponding to rat VGLUT2 (Abcam, ab79157) and has been previously shown to stain VGLUT2 in zebra finches (86) ...
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bioRxiv - Neuroscience 2020Quote: ... The signal intensity from the examined proteins were evaluated on free-floating sections using the standard method of simultaneously combined staining (Abcam Protocol). Brain slices (50 μm ...
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bioRxiv - Cancer Biology 2020Quote: ... were resolved by SDS-PAGE and the target proteins were detected with Western blot (WB) using the antibodies as follows: GAPDH Rabbit monoclonal antibody (ab181602, Abcam, UK), SLC40A1 Rabbit polyclonal antibody (ab58695 ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were blocked for 1 hour at room temperature with 5% fat-free milk or milk-free blocking buffer (for analysis of phosphorylated proteins) and incubated overnight at 4°C with primary antibodies for p-GCN2 (Abcam, ab75836), p-eIF2α (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: Growth factor proteins were analysed in conditioned medium and dissociative tissue extracts by Western blotting using anti-HDGF from AbCam (ab131064), anti-CTGF from Santa Cruz (sc-14939 ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin levels were determined in duplicate using 150 μg of protein lysates per well using a sandwich ELISA assay (abcam, ab252364). Western blot and qPCR analyses were carried out as described above ...
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bioRxiv - Microbiology 2022Quote: ... Spain) binding to the 229E nucleocapsid protein and a goat anti-mouse secondary antibody conjugated with fluorescein isothiocyanate (Abcam, United Kingdom). TCID50 titers were calculated according to the method of Reed and Muench (44).
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bioRxiv - Microbiology 2020Quote: ... endogenous human NPC1 and HSP90 were purified using immobilized Recombinant Protein G Resin (Generon) and 4 µg of specific antibodies against NPC1 (Abcam, ab108921) or HSP90 (Enzo Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... deparaffinized sections were rehydrated and stained using specific antibodies against Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5, ab219107, Abcam, USA), Lysozyme (ab76784 ...
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bioRxiv - Developmental Biology 2019Quote: Phospho-protein staining was performed as previously described (Okuda et al. 2018) using the following antibodies: GFP (abcam cat. no. ab13970), pErk1/2 (cell signaling technologies cat ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin immunoprecipitation was performed by incubation of the chromatin fraction overnight with 20 μl of protein-A coated beads (Thermo-Scientific, 53139) and 2 μg of H3K4me1 specific (Abcam, ab8895) or 2 μg of H3K4me3 specific (Abcam ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Microbiology 2020Quote: ... or IgG were used to immunoprecipitate the chromatin fragments in the supernatant by overnight incubation using protein A sepharose beads (Abcam, UK) at 4 °C.The beads were washed twice with wash buffer ...
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bioRxiv - Microbiology 2021Quote: ... Proteins co-purified with His-NS5A or His tag protein were detected by western blot with mouse anti-Myc tag monoclonal antibody (Abcam, USA) for probing NEMO and mouse anti-His tag monoclonal antibody (Abcam ...
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was extracted from the 8 cultures using the lysis buffer from the GFP Fluorescent ELISA Kit (ab229403) (Abcam, Cambridge, UK) that was supplemented with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated with primary rabbit anti-SARS nucleocapsid protein antibody(NB100-56576, Novus, USA) overnight at 4°C and then with goat anti-rabbit IgG HRP (Abcam, UK) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: The quantitative detection of serum lipopolysaccharide binding protein (LBP) was performed by Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (Abcam, ab213876) according to the manufacturer protocol ...
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bioRxiv - Physiology 2022Quote: ... 50-100 μg of protein were used for the pull down of FLAG-tagged Kcnq4 proteins with monoclonal anti-FLAG M2 antibody produced in mouse (mouse monoclonal Anti-FLAG M2 antibody, F3165) and Protein G Sepharose beads (Abcam, UK) at 4 °C overnight ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the included Pierce™ Protein A/G Plus agarose beads were incubated with rabbit anti-myc polyclonal antibody (ab9106, Abcam, USA) and cross-linked using 2.5 mM DSS crosslinker ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was transferred to a fresh Eppendorf tube and the protein concentration was assessed using Bradford reagent (Abcam, cat#ab119216) and stored at -80°C when not used immediately ...
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bioRxiv - Molecular Biology 2023Quote: ... were coupled with 10μg of anti-protein of interest (POI; anti-Foxg1 ChIP-grade, rabbit polyclonal, Abcam #ab18259; anti-GFP, rabbit polyclonal, Abcam #ab290), or 10μg of rabbit IgG (Millipore #12370 ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Molecular Biology 2023Quote: ... which was performed overnight at 4°C by incubating the shared DNA-protein complex with 10 µg of anti-ADNP (Abcam Ab231950) antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... nucleoplasm and chromatin fractions were used for protein analysis with western blotting with the following antibodies were used: anti-DHX9 (Abcam 26271), anti-TBP (Abcam 818) ...
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bioRxiv - Genetics 2022Quote: ATPase activity of cohesin was measured using EnzChek Phosphate Assay Kit with purified recombinant proteins depleted of free phosphate using Inorganic Phosphate Binding Resin (Abcam: ab270547). Reactions were assembled with10nM cohesin ...
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bioRxiv - Biochemistry 2023Quote: ... Denatured samples were resolved in 4-12% Bis-Tris SDS-PAGE gradient gels and stained with InstantBlue® Coomassie protein stain (Cat#ab119211, abcam). Protein bands were excised from gel ...
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bioRxiv - Cancer Biology 2023Quote: ... E0771 breast cells were treated with 0 and 100 ng/ml of active mouse recombinant Ifng protein (Abcam, catalog number: 259378) for 24 ...
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bioRxiv - Neuroscience 2023Quote: ... the cryosections of human brain were mounted on a Polyethylene naphthalate (PEN) Membrane Slide (Zeiss, 415190-9041-000) and then stained with spike protein antibodies using the IHC kit (Abcam, ab210062). After further processing ...
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bioRxiv - Microbiology 2023Quote: ... Cells were blocked with Dako protein block then stained for cell surface Env with 2G12 anti-gp120 directly conjugated to APC (Abcam, ab201807) and diluted 1:100 in Dako antibody diluent (10ug/mL final concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by separation using sodium dodecyl sulfate polyacrylamide gel electrophoresis (4–12% polyacrylamide) and staining with InstantBlue Coomassie Protein Stain (Abcam, UK). Fractions containing chalcone synthase were combined and loaded onto a HiLoad 16/600 Superdex 200 pg column ...