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Citations for abcam :
3101 - 3150 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... incubated overnight at 4°C with primary antibodies (anti-HA, 1:1000, BioLegend, #901502; anti-myc, 1:3000, Abcam, #ab9106; anti-Histone H3, 1:5000, Abcam, #ab1791), washed ...
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bioRxiv - Biophysics 2020Quote: ... All sections were permeabilized with 0.2% Triton X-100 in PBS for 15 minutes and non-specific protein binding was then blocked by incubation in a commercial blocking agent (ab156024, Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies against phospho-Heat Shock Protein 27 (S82) and phospho-c-Jun (S73) were obtained from Abcam (Cambridge, United Kingdom), while phospho-JNK (T183/Y185 ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted proteins were analyzed on a 6% SDS-PAGE gel followed by a western blot using monoclonal anti-GST (Abcam) or anti-MBP (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... one piece corresponding to the higher protein ladder size for the incubation of RAD21 and CTCF primary antibodies (RAD21: anti-RAD21 antibody, rabbit polyclonal, Abcam, ab154769 ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Physiology 2019Quote: ... rabbit acetylated SOD2 (K122), rabbit SOD2, and rabbit OXPHOS cocktail (to analyze NDUFB8, SDHB and UQCR2 protein content) from Abcam. Fluorescent anti-mouse or anti-rabbit secondary antibodies (red ...
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bioRxiv - Biochemistry 2019Quote: ... and equal amounts of proteins were used for each condition that was analyzed by immunoblotting with following antibodies: UCHL1 (ab27053; Abcam), Tubulin (2148 ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for SARS-CoV2 N protein using Alexa488-labelled-CR3009 antibody produced custom (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (ABCAM). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Cancer Biology 2021Quote: ... for 6 h and then fixed with methanol for immunostaining with an antibody against nuclear pore complex proteins (clone mAb414, Abcam) following the regular immunofluorescence protocol described below ...
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bioRxiv - Cancer Biology 2019Quote: ... 30 μg of protein lysate from each fraction was analyzed by western blotting using the anti-HER4 antibody E200 (Abcam) to determine HER4/4ICD subcellular localization ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Cell Biology 2021Quote: ... ovalbumin and CD4-Fc fusion proteins were labelled with allophycocyanin (APC) with the Lightning-Link Labelling Kit (Abcam, Cambridge, UK).
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of total protein extract were used to IP with either 2.5 µg V5 rabbit polyclonal antibody or VPS35 mouse monoclonal over night at 4°C and recovered after incubating for 6h at 4°C in a rotator with equilibrated Agarose Protein-G beads (Abcam). Immunoprecipitation followed by MS analysis was performed as described but using magnetic Protein G beads (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... PBS for 45 minutes and further incubated in blocking solution containing anti-SARS-CoV-2 spike protein subunit 2 (Abcam) 1:1000 dilution for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Iron saturation of transferrin was measured on 6% TBE-urea gels as previously described.32 Gels were stained with InstantBlue Coomassie protein stain (abcam).
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpression of WT and mutant c-Myc protein in cells were confirmed by immunofluorescence staining of transfected cells with anti-c-Myc antibody (Abcam). The sequences of cloning primers were listed in Table S4.
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Biochemistry 2022Quote: ... The supernatants were incubated with an anti-TIN1 antibody followed by protein-A/G-agarose beads (Abmart) or with anti-GFP Sepharose beads (Abcam) at 4 °C for 6 h ...
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bioRxiv - Biochemistry 2022Quote: ... The molecular masses of the protein bands were assessed by comparing with the mass of the bands in the lane containing the standards (Abcam). The experiment was performed in triplicate and the bands corresponding to the heterodimer and heterotetramer were visualized in all the three experiments.
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bioRxiv - Biochemistry 2022Quote: ... Separated proteins were transferred to polyvinylidene difluoride (PVDF) membranes and immunoblotted with antibodies specific for SF3B1 (Abcam, catalog no. ab170854), SF1 (Bethyl Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... 100µg of cleared chromatin (protein) was incubated with 2µg of the following antibodies overnight at 4°C: H3K27ac (Abcam, ab4729), H3K4me1 (Abcam ...
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bioRxiv - Immunology 2020Quote: The iNOS recombinant protein (full length) was procured from sigma and Nitric Oxide Synthase activity assay kit was from Abcam. The NOSIP (full length ...
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bioRxiv - Immunology 2021Quote: ... AD-01 (NH2-QIRQQPRDPPTETLELEVSPDPAS-OH) (Almac Group, United Kingdom), ALM201 (NH2-IRQQPRDPPTETLELEVSPDPAS-OH) (Almac group, United Kingdom) and recombinant FKBPL protein (ab98117; Abcam). All experiments were carried out at 37°C in a humidified atmosphere of 95% O2/5% CO2.
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bioRxiv - Microbiology 2020Quote: ... Co-IP for NPC1 was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against NPC1 (Abcam, ab108921). The cell pellets were incubated for 30 minutes on ice with 200μl lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then mixed with either anti-HA magnetic beads (Thermoscientific) or protein G magnetic beads (Thermoscientific) coupled with anti-pADPr (Abcam) (beads were pre-blocked with 1% BSA) ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were permeabilized with 0.1% Triton X 100 in 1X PBS for 5 min and blocked with Protein Block (Abcam, USA) for 20 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... were washed with lysis buffer (containing protein inhibitors) and incubated with 5 µg of 4C4 antibody or IgG1k as a control isotype (ab18443 Abcam) at 4°C overnight under rotation ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS and stained overnight at 4°C with ACE2 protein-specific antibody (Abcam Ab15348). Cells were then incubated with anti-rabbit secondary antibody (Alexa Fluor 536 anti-rabbit ...
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bioRxiv - Neuroscience 2020Quote: ... 2019) using antibodies against cAMP response element-binding protein (CREB; clone LB9, produced in mouse, #ab178322, AbCam, Cambridge, UK; RRID:AB_2827810), phospho-CREB (Ser133 ...
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bioRxiv - Physiology 2021Quote: ... Blots were incubated overnight in rabbit anti-mouse primary antibody for proteins of interest (Supplemental Table S4) and with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibodies (ab6721, Abcam). Proteins of interest were detected by incubating blots in ClarityTM Western ECL Blotting Substrate (1705061 ...
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bioRxiv - Molecular Biology 2019Quote: ... primary rabbit raised antibodies against pCRKL Y207 (37 kDa) and pCRK Y221 (34 kDa) (another downstream target protein) were procured from Abcam plc ...
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bioRxiv - Genomics 2021Quote: ... Chromatin immunoprecipitation was performed by incubating the pre-cleared chromatin with 75 μL Dynabeads Protein G bound with 7.5 μg H3K27ac antibody (Abcam ab4729) overnight at 4°C rotating ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 infected cells were visualized using SARS-CoV-2 S-protein specific immunochemistry staining with anti-SARS-CoV-2 spike glycoprotein S1 antibody (Abcam) 90.
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bioRxiv - Immunology 2021Quote: ... and hLIGHT-6×His tag proteins (made by the methods described above) in the mixtures with anti-6×His tag PE-labeled antibody (Abcam) for 20 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated by SDS-PAGE and subjected to Western blotting with antibody against S14 phosphorylated Syx (Abcam; ab63571), using the ECL detection system (Pierce Protein Research Products ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated by SDS-PAGE and subjected to Western blotting with antibody against Syx phospho-S14 (Abcam; ab63571). Digitized scans were derived by PhosphorImager (Molecular Dynamics ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... mCherry was identified based on its auto fluorescent signal while the luciferase protein was immuno-stained with anti-firefly luciferase antibody (Abcam) labeled with Zenon AF647 Mouse Labeling Kit (Life Technology) ...
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bioRxiv - Biochemistry 2021Quote: ... Clones were validated for GPC1/5 knockouts via Western analysis following heparinase III digestion using a monoclonal antibody (3G10) direct against the glycosylation attachment site in the core protein structure (370260, abcam) or via sequencing (GPC1 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 2 hours with: (i) chicken polyclonal antibody anti microtubule-associated-protein 2 (MAP-2) (Abcam, #Ab5392) at dilution of 1/1000 in PBS containing 1% fetal calf serum and 0.1% of saponin (this antibody stains specifically cell bodies and neurites ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates containing 200 µg of total protein were incubated with anti-ERK (CST) or anti-DUSP6 (Abcam, Cambridge, UK) antibody at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), CLIC4 (Santa Cruz) ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-G3BP1 beads were generated by incubating 200 μL Protein-G beads with 20 μL anti-G3BP1 antibody (Abcam, ab290) in 1 mL Buffer B (200 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The gels were run in MOPS buffer at 200 V for 45 minutes and stained with InstantBlue Coomassie protein stain (Abcam). Protein bands intensities were quantified using ImageJ76 and then plotted against fraction numbers using GraphPad Prism version 9.0.1.
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were separated by 12 % SDS-PAGE and immunoblotted with anti-GST or anti-His antibody (Abcam, Cambs, England).
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were then transferred to a nitrocellulose membrane and blotted with an anti-RFP antibody (Abcam No. 34767) at a dilution of 1/2,000.
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bioRxiv - Physiology 2022Quote: ... Samples were transferred to PVDF membranes that were subsequently probed with the following antibodies for protein and phosphoprotein detection: anti-PIK3CA (ab40776, Abcam), anti-PDPK1 (ab52893 ...
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bioRxiv - Molecular Biology 2022Quote: ... The same samples without BS3 were run as a loading control and stained using a coomassie protein stain (Abcam ab119211) because detection via our anti-DnaD antibody (Eurogentec ...