Labshake search
Citations for abcam :
3051 - 3100 of 3291 citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
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bioRxiv - Molecular Biology 2023Quote: SA-bGAL activity was evaluated in the doxorubicin-induced senescent cells after 10 days using the Senescence Detection Kit (Catalogue number: ab6535, Abcam) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Metabolic activity within periosteal MSC was quantified using a Cell Counting Kit 8 assay (WST-8 / CCK8; Abcam, Cambridge, UK). Water soluble tetrazolium salts are used to quantify cell viability through the production of formazan dye similar to that of an MTT assay ...
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bioRxiv - Immunology 2024Quote: ... and were assessed in BMDM and RAW264.7 macrophage cells following exposure to 2-AA at the times indicated and by utilizing the ATP Assay kit (cholorimetric/fluorometric) (#ab83355, Abcam) and the PicoProbe AcCoA assay kit (ab87546 ...
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bioRxiv - Cell Biology 2024Quote: ... adult mouse cardiomyocyte cell line HL-1 or primary neonatal mouse cardiac fibroblasts (NMCF) or cardiomyocytes (NMCM) using the Histone extraction kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The amount of cholesterol in cells and supernatants was assessed using the cholesterol/cholesteryl ester assay quantification kit (ab65359, abcam) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Cellular ROS was also detected using the DCFDA/H2DCFDA cellular ROS assay kit according to the manufacturer’s instructions (Abcam; ab113851). To analyze cell populations ex vivo ...
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bioRxiv - Immunology 2024Quote: ... spent media was collected for each condition and lactate secretion was assayed in duplicate or triplicate using either Lactate Colorimetric/Fluorometric kit (abcam) or Lactate-Glo (Promega ...
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bioRxiv - Cell Biology 2023Quote: APOB protein levels in RPE and retinal lysates were measured using an Apo B ELISA Kit (ab230932, Abcam Cambridge, UK). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... 45 μL of reaction mixtures were added to 100 μL of glutamate detection reaction mix (Abcam Glutamate Assay Kit, ab83389) in a 96-well plate ...
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bioRxiv - Developmental Biology 2022Quote: ATP synthase activity of wild-type and stat3 mutant zebrafish embryos was measured using the ATP Synthase Specific Activity Microplate Assay Kit (Abcam) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: The activity of lysyl oxidase (LOX) in CM samples (n = 27) was measured using fluorometric commercial assay (Lysyl Oxidase Activity Assay Kit, Abcam) following the manufacturer’s protocol ...
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α-synuclein expression in response to bacterial ligands and metabolites in gut enteroendocrine cellsbioRxiv - Neuroscience 2023Quote: The amount of α-synuclein in the culture medium was measured using a mouse α-synuclein ELISA kit (Abcam ab282865) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and native histones that contain the modification and were purified from HEK293T cells using a Histone Extraction Kit (Abcam ab113476). Protein concentration was determined using the Pierce BCA assay ...
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bioRxiv - Molecular Biology 2023Quote: ... gel followed by either silver staining with Pierce Silver Stain Kit (Thermo) or Western blotting against anti-NOP58 (#ab236724, Abcam) and anti-GAPDH (#5174s ...
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bioRxiv - Neuroscience 2023Quote: ... were labeled with the fluorescent dye Alexa Fluor 647 according to the manufacturer’s protocol (Alexa Fluor 647 conjugation kit lightning link, Abcam, ab269823). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... Blood was then centrifuged at 1500 x g for 10 minutes and plasma was collected and analyzed with a TAT ELISA kit (Abcam) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were transferred to a 96-well plate and the amount of inorganic phosphate released from the hydrolysis of NTP was determined using Phosphate Assay Kit PiColorLock (Abcam) according to protocol by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of changes in mitochondrial membrane potential was determined following the protocol of the TMRE-Mitochondrial Membrane Potential Assay Kit (Abcam). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PSA levels were quantified both in the conditioned media and in the mouse sera using the PSA ELISA kit (Abcam), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Total nitrite/nitrate was measured as a proxy for nitric oxide concentration using the Nitric Oxide Assay Kit (Abcam, ab65327), with 5 hours of incubation with the enzyme cofactor to permit more than 99% conversion of nitric oxide to nitrite.
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bioRxiv - Molecular Biology 2023Quote: ... cell viability was evaluated at the end of incubations by a dehydrogenase activity assay (Cell Counting Kit 8, Abcam #ab228554), and expressed relative to the solvent control.
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bioRxiv - Microbiology 2023Quote: ... A bacterial culture containing about 109 exponentially growing Leptospira was used to determine the catalase activity using the Catalase Activity Assay Kit (Abcam), according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated with the polymer rabbit detection system (DCS) and phH3 staining was detected after incubating the slides with DAB substrate kit (Abcam) for 10 min at RT ...
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bioRxiv - Immunology 2023Quote: C3 or C5 levels in feces or serum of mice were measured with a Mouse C3 ELISA kit (Abcam, ab157711) or a Mouse C5 ELISA kit (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... Quantitative detection of IgM/IgG in exosomes was performed according to the manufacturer’s instructions (IgM/IgG cow ELISA kit, Abcam, UK). The absorbance was determined on a plate reader using the contents of each well at 450 nm ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-1D4 Ab and anti-OLLAS Ab were conjugated to PE using an Ab conjugation kit from Abcam (cat. 102918) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: The Dihydroethidium (DHE) assay kit for the measurement of intracellular superoxide and hydrogen peroxide was purchased from Abcam (Cat#ab236206). The MitoSOXTM for the measurement of mitochondria superoxide was purchased from ThermoFisher Scientific (Cat#M36008) ...
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bioRxiv - Developmental Biology 2023Quote: EdU (5-ethynyl-2′-deoxyuridine) labeling assay was performed according to the instruction of EdU Assay / EdU Staining Proliferation Kit (ab219801, Abcam). Proliferating cells were labeled by injecting 10mg/kg BW EdU to mice intraperitoneally 2 hours before sacrifice ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 μL of Annexin V-FITC and 5 μL of propidium iodide were added into each tube (Annexin V-FITC Apoptosis Staining/ Detection Kit, Abcam). The tubes were incubated at RT for 5 min in the dark ...
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bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: Changes in mitochondrial membrane potential produced by I3C and BNF in the five cancer cell lines was determined with the tetramethylrhodamine ethyl ester (TMRE) Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: RCC243 cells were treated with 5 μM MS023 for the indicated time periods and then cells were trypsinized and stained using the Propidium Iodide Flow Cytometry Kit (Abcam, #ab139418, Abcam) based on the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... Ferric Reducing Antioxidant Power (FRAP) assay of maternal plasma was done according to manufacturer instructions (FRAP Assay Kit, colorimetric, ab234626, Abcam).
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bioRxiv - Physiology 2023Quote: ... cells were equilibrated to room temperature and stained with PI for 30 minutes at 37°C using Propidium Iodide Flow Cytometry Kit (ab139418; Abcam) according to the manufacturer’s protocol and incubated at 37°C in the dark for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... non-esterified fatty acids (NEFA) and glycerol were determined using enzymatic kits (Triglyceride SL, Sekisui Diagnostics; NEFA-HR, Wako; glycerol, ab65337 Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Free glycerol was measured in the infranatant fraction of the isolated adipocyte culture using a colorimetric assay kit (ab65337, Abcam) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: EA.hy926 cells or EZH2 WT/mutant overexpressing HEK-293 treated with NO donor SNP (500 µM) for 2 hours were processed with the help of a Biotin Switch Assay kit (Abcam) using the standard manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: Gamma-irradiated infected and non-infected tissue specimens were homogenized on ice using Dounce Glass Manual Tissue Grinder (Etcon) following the Plasma Membrane Protein Extraction Kit protocol (Cat# ab65400, Abcam). Total protein concentration was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Separation of U2OS cellular compartments was performed according to the manufacturer’s instructions with a Standard Cell Fractionation kit (abcam # ab109719). Two different markers were used to assess the purity of each fraction ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and stored at -80°C or further concentrated using a polyethylene glycol (PEG) virus precipitation kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... that occurred per minute of incubation with each substrate at 37°C by Aspartate Aminotransferase Activity Assay Kit (Abcam ab105135) and the Alanine Transaminase Activity Assay Kit (Abcam ab105134) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were lysed in 80 μL of ice-cold buffer from Glutathione Assay Kit (catalog no: ab239709, Abcam). The lysed cells were kept on ice for 10 minutes followed by mixing thoroughly with 20 μL of 5% SSA (sulfosalicylic acid ...
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bioRxiv - Microbiology 2023Quote: Cell apoptosis resulting from the treatment with carbonic anhydrase inhibitor – S4 was evaluated using the Annexin V-FITC Apoptosis Staining/Detection Kit (ab14085) according to the manufacturer’s protocol (Abcam Inc.). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: We measured mitochondrial membrane potential (MtMP) using the JC-1(tetraethylbenzimidazolylcarbocyanine iodide) mitochondrial membrane potential assay kit (Abcam; Cat #113850) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: The acetyl-CoA content of the cell culture medium was determined with the Acetyl-CoA Assay Kit (Reference: ab87546, Abcam) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... the resulting supernatant fractions were boiled for 10 min to inactivate enzymatic activity and their glycogen content measured using a Glycogen Assay Kit (ab65620, Abcam). Briefly ...