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Citations for abcam :
2951 - 3000 of 3291 citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cell lines were validated by phenotypic screening and confirmed to be mycoplasma-free using a PCR detection kit (Abcam). All cells were maintained at 37 °C in a 95% humidity and 5% CO2 atmosphere.
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bioRxiv - Microbiology 2023Quote: ... The amount of histamine released into the culture supernatant was determined using a histamine detection kit (Abcam, Cambridge, MA) as described previously [19] ...
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bioRxiv - Neuroscience 2023Quote: ... cells grown in the same 96-well plate were stained using the Janus Green Cell Normalization Staining Kit (Abcam). OD values at 595 nm were measured by a Labsystems Multiskan Bichromatic plate reader (Titertek Instruments ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... assay was performed on paraffin embedded tissue sections according to the manufacturer’s instructions to detect DNA fragmentation in apoptosis (TUNEL assay kit-HRP-DAB, Cat#: ab206386, Abcam). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... Intracellular lipid accumulation of all viable clones was quantified with Oil Red O Stain Kit (ab150678; Abcam, Cambridge, UK) on day six (Supplementary Fig ...
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bioRxiv - Genomics 2024Quote: ... and ∼10 µg total protein was used to measure TET activity using the TET Hydroxylase Activity Quantification Kit (Abcam) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: Islet were washed once with PBS and cholesterol labelled according to the Cholesterol Assay kit (cell-based) (ab133116, Abcam). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... cAMP levels in the cells were determined in a plate reader by using fluorescence-based cAMP detection kit (Abcam) according to the manufacturer’s recommendations and normalized to the total protein present in the samples as determined by a BCA protein estimation kit (Pierce) ...
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bioRxiv - Microbiology 2024Quote: The amount of ATP generated in the gastrocnemius muscle samples was measured using the ATP assay kit (Abcam, 83355) described in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... in mouse brain cortical lysates (including hippocampus) was determined by competitive ELISA kit according to the manufacturer’s instructions (abcam).
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bioRxiv - Neuroscience 2024Quote: ... Tissue ferrous (Fe2+) and total iron content were determined using the colorimetric Iron Assay Kit (Abcam, Cat. No. ab83366) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells under respective conditions were stained with cathepsin D and L kits (Abcam, Cat# ab65302 and ab270774) following company protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were further processed using rabbit specific HRP/DAB (ABC) detection IHC kit according to the manufacturer’s protocol (Abcam). Slides were subsequently scanned on an Akoya Vectra Polaris scanner scanning at ×20 magnification and semi-quantitative analysis of cytoplasmic FASN ...
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bioRxiv - Molecular Biology 2024Quote: ... LDH levels were quantified in cell supernatants using an LDH-Cytotoxicity Assay Kit following manufacturers’ protocol (abcam, United Kingdom). To quantify caspase-3/7 activity ...
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bioRxiv - Neuroscience 2024Quote: ... IL-6 and IL-10) were measured by Sandwich ELISA according to recommendations of the kit’s manufacturer (Abcam, UK): Mouse IL-6 Matched Antibody Pair Kit (# ab213749) ...
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bioRxiv - Cell Biology 2024Quote: ... The levels of Citrine-H3 (Cit-H3) and myeloperoxidase (MPO) were determined by ELISA kits (Abcam, MA & Cayman, MI).
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bioRxiv - Physiology 2024Quote: Sphingomyelin levels in mAoECs after treatment with vehicle or wortmannin was determined by a sphingomyelin assay kit (abcam, ab138877) while following manufactures instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Mitochondrial fractions (for cytochrome c measurement) and membrane fractions (for Myr-RSK3 measurement) were extracted from cells using a Cell Fractionation kit (Abcam) and a Mem-PER™ Plus Membrane Protein Extraction Kit (Thermofisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 1X Binding buffer with Annexin V and propidium iodide (PI) with a Annexin V-FITC Apoptosis Detection Kit (Abcam), according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... adherent and floating cells were collected and labeled with the Annexin V-Cy3 Apoptosis Detection Kit (Abcam, Cambridge, UK, #ab14143) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Fibrinogen was measured in P10 serum (6 Jag1+/+ and 6 Jag1Ndr/Ndr mice) using a commercial kit (cat. #ab213478, Abcam), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Pro-inflammatory cytokine Interleukin-lβ (IL-1β) present in supernatants was measured using an appropriate ELISA kit (ab100767, abcam, UK), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... to avoid bias by color changes in the medium and inhibition of growth respectively total cellular viability was assayed using the MTS Assay Kit (Abcam). 100% viability was defined as the readout from non-treated cells (addition of PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... gel followed by either silver staining with Pierce Silver Stain Kit (Thermo) or Western blotting against anti-Nop58 (#ab236724, Abcam) and anti-GAPDH (#5174s ...
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bioRxiv - Cell Biology 2019Quote: ... oryzae conidia were harvested and normalized using the Iron Assay Buffer (100 ml) from the Iron Assay kit (Abcam, ab83366), to reach a final conidia count of 3×105/well ...
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bioRxiv - Biochemistry 2019Quote: Cathepsin D activity (used as a proxy for total lysosomal hydrolase activity) was measured by using Cathepsin D Activity Assay Kit (Abcam). Cells were collected ...
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bioRxiv - Cell Biology 2019Quote: Cells treated with 0.1% DMSO or 5 μM SGC-0946 were stained with Annexin V-FITC and DAPI following the protocol of the Annexin V-FITC Apoptosis Detection Kit (Abcam). Fluorescence was detected by FACS using the CyAn ADP Analyzer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were lysed and the level of PKC-specific kinase activity was measured in 30 μg cell lysate using the PKC Kinase Activity Assay Kit (AbCam) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... 21 Gclc KO and Gclc WT mice were anesthetized and eyes enucleated and immediately assayed using the GSH/GSSG Ratio Detection Assay Kit (Fluorometric-Green) (Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The culture supernatants containing pseudotyped viruses particles were either used immediately or flash frozen in aliquots and stored at 80°C until use after being concentrated using a PEG virus precipitation kit (Abcam). Incorporation of the SARS-CoV-2 S protein into the SARS-CoV-2pp was confirmed using Western Blot analysis with anti-SARS-CoV-2 S1 chimeric monoclonal antibody with combined constant domains of the human IgG1 molecule and mouse variable regions (40150-D001 Sinobiological ...
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bioRxiv - Bioengineering 2021Quote: ... stained with 10 µl of AnnexinV-FITC and propidium iodide (PI) following manufacturer’s instructions (Annexin-V Apoptosis Staining Detection Kit, ab14084, Abcam, USA). Metabolic activity tests were performed using an alamarBlue cell viability assay (BUF012 ...
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bioRxiv - Cancer Biology 2020Quote: The relative MDA concentration in cell or tumor lysates was assessed using a Lipid Peroxidation (MDA) Assay Kit (Abcam, #ab118970) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The amount of ATP released into the medium was measured with an ATP Assay Kit (Fluorometric, ab83355; Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... RNA (1 μg) was used to synthesize cDNA from each donor using the high-capacity reverse transcriptase cDNA synthesis kit (Abcam). DAT ...
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bioRxiv - Bioengineering 2021Quote: ... sfGFP levels were assessed from similarly prepared tumor homogenates and matched serum drawn from terminal cardiac-puncture using a GFP ELISA kit following manufacturers instruction (abcam).
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bioRxiv - Cell Biology 2021Quote: ... ovalbumin and CD4-Fc fusion proteins were labelled with allophycocyanin (APC) with the Lightning-Link Labelling Kit (Abcam, Cambridge, UK).
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bioRxiv - Microbiology 2021Quote: Culture media and cellular extracts were collected and centrifuged in preparation for LDH activity quantitation with a commercially available kit (#ab102526, Abcam). LDH activity was measured by a microplate reader (Tecan Infinite M200 Pro ...
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bioRxiv - Bioengineering 2020Quote: ... hCMEC/D3 and primary rodent microglia cells were labeled with Cell Tracking Dye Kit - Green - Cytopainter (ab138891, Abcam, Cambridge, UK) and Cell Tracking Dye Kit - Deep Red - Cytopainter (ab138894 ...
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bioRxiv - Immunology 2021Quote: ... the HIV Env 1086.c K160N gp120 protein was conjugated to biotin using the Fast Type A Biotin Conjugation kit (Abcam) and then captured on avidin-labeled fluorescent beads (NeutrAvidin™ ...
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... cytotoxicity and killing potential was measured by LDH amount in the cell supernatant using the LDH-Cytotoxicity Assay Kit (Ab65393, Abcam) following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2022Quote: ... Enzyme activity was assessed on the basis of glutamate production as measured with a glutamate assay kit (Abcam, Cat# 138883). The IC50 value was estimated from biological quadruplicates with a four-parameter logistic model63 and with the use of JMP pro 15 software (version 15.1.0).
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Cancer Biology 2022Quote: Cytotoxicity and apoptosis were assessed using the Annexin V-CF Blue 7-Aminoactinomycin D (7-AAD) Kit (Abcam Cat# AB214663) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL) staining was performed using TUNEL assay kit (Abcam, ab206386) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: The iNOS recombinant protein (full length) was procured from sigma and Nitric Oxide Synthase activity assay kit was from Abcam. The NOSIP (full length ...
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bioRxiv - Cancer Biology 2021Quote: Cell transformation assays in immortalized HBEC hTERT were performed using the Cell Transformation Assay Kit-Colorimetric (Abcam Cat No. ab235698). Based on manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... SOD isoenzyme activity was measured according to the manufacturer’s instructions in the SOD activity assay kit (Abcam, cat. n. ab65354).
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bioRxiv - Plant Biology 2021Quote: Quantitative measurement of reactive oxygen species was carried out using DCFDA cellular ROS detection assay kit (Abcam, product number AB113851), and the assay was carried out following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Aconitase activities in cytosolic and mitochondrial fraction of the brain cortex lysates were measured using Aconitase Enzyme Activity Microplate Assay Kit (Abcam) according to manufacturer’s instructions.