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Citations for abcam :
3001 - 3050 of 3162 citations for 2 4 Methoxyethoxy 3 methyl 2 pyridinyl methylthio benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked for 30 min with 3% BSA dissolved in Tris-buffered saline with 0.1% Tween (BSA-TBST) and incubated overnight at 4 °C with primary antibody (ab43177 chicken-developed anti-ATP synthase β, Abcam plc) diluted 1:5000 in BSA-TBST ...
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bioRxiv - Immunology 2020Quote: ... The sonicated nuclear fractions were divided for input control and for overnight incubation at 4℃ with 10 μg of either anti-C/EBPβ (Abcam cat. 32358) or the negative control IgG ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies diluted in 1% NGS blocking buffer were added to the cultured cells overnight at 4°C (Oct4, 1:100, Santa Cruz; β-III-tubulin 1:500, Covance; TH, 1:1000, Chemicon; Serotonin, 1:500, Abcam). Negative primary controls consisted of cells treated with blocking buffer without the addition of primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... The retinas were incubated in PBS with 1% Triton X-100 and 0.5% Tween 20 for 1 h at room temperature and in 4% BSA for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies: RBPMS (1:500, Abcam, Cambridge, UK), ChAT (1:100 ...
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bioRxiv - Physiology 2022Quote: ... 4 µm serial sections of paraffin-embedded slides were incubated with rat anti-mouse CD34 (MEC 14.7, monoclonal, Abcam, Cambridge, United Kingdom) at a 1:200 dilution overnight at 4° ...
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bioRxiv - Genetics 2022Quote: ... After 6 h the cells were washed in 1XPBS and fixed in 4% PFA followed by staining with rabbit polyclonal beta COP antibody (ab2899, Abcam, Cambridge, UK) after permeabilising with 0.5% Triton-X 100 ...
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bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
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bioRxiv - Immunology 2022Quote: ... for 30 minutes at 4°C and then incubated with the titrated primary anti-MPO-PE and primary citrullinated histone H3 (citH3) antibody (Abcam, Waltham, MA) for 30 minutes at 4° C ...
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bioRxiv - Physiology 2022Quote: ... Membranes were incubated in the following primary antibodies overnight at 4°C: polyclonal rabbit anti-beta actin IgG (Abcam Cat# ab8227, RRID:AB_2305186) at a dilution of 1:1,000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... sections were incubated overnight at 4°C in 1:100 dilution of rabbit polyclonal anti-nitro tyrosine antibody (ab42789; Abcam, Cambridge, MA). Slides were rinsed in PBS and developed using polyclonal goat anti-rabbit Immunoglobulins conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 24 hr in blocking buffer containing a chicken anti-mCherry antibody (1:500, ab205402, Abcam, Cambridge, MA). After PBS washes ...
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bioRxiv - Genetics 2021Quote: ... embryos were washed 3 times 10 minutes in 1x PBS with 0.1% Triton-X100 and 0.1% BSA and incubated overnight at 4°C with the secondary antibody goat-anti-mouse A568 1:500 (Abcam, antibody ID# ab175473). On the 4th day ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated on an orbital shaker for 24h at room temperature + 24h at 4 °C in polyclonal rabbit anti-mCherry (1:30 000, Abcam, cat. ab67453) or polyclonal chicken anti-EYFP (1:200 000 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Cell supernatant was collected 24h after changing cell medium then immediately filtered by centrifugation for 15 minutes at 4°C on a 10KDa column (Abcam or Millipore). This step eliminates proteins ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: Pull-down experiments were performed by following the method previously described.36 15-20 μg of acid extracted histones or nucleosomes isolated from HeLa cells were mixed with 50 μM of recombinant His6-tagged-BRPF2 bromodomain incubated at 4°C on a nutator for 60 min in the presence or absence of 10 μM BAY- 299 (abcam cat# ab230368) inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with the following primary antibodies: KLF4 (R&D, AF3640) and cyclophilin B (abcam, catalog ab16045). Chemiluminescent detection was performed with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was washed in TBST and incubated for 45 min in TBST containing 4% dried milk and HRP mouse anti-beta Actin AC-15 (1:25000, abcam Ref. ab49900). The membrane was washed in TBST and incubated 1 min in ECL Western Blotting Substrate (Thermo Fisher Scientific Ref ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12 ...
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bioRxiv - Microbiology 2022Quote: ... Mitochondrial membrane potential was measured using a tetramethylrhodamine ethyl ester (TMRE) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) as a control (Abcam, Cambridge, UK). Prior to incubation and acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated overnight at 4°C in the same solution containing a rabbit-anti-choline acetyltransferase (ChAT) monoclonal antibody (1:1000, Abcam ab178850, RRID:AB_2721842) and a guinea pig-anti-NeuN polyclonal antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; GAPDH ...
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bioRxiv - Physiology 2022Quote: ... primary antibodies were added in 50 μL blocking buffer for 12 hours at 4° C: ATP synthase antibody (Abcam, ab128743 1:200), sAC antibody (R21 IHC ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then incubated in the following primary antisera overnight at 4°C in PBT: guinea pig antiinsulin (Abcam; Waltham, MA, USA), rabbit anti-glucagon (Santa Cruz ...
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bioRxiv - Pathology 2024Quote: ... Membranes were then blocked for 1 hour with 5% dry milk in PBST (PBS supplemented with 0.1% Tween-20) buffer and incubated overnight at 4°C with primary antibody (anti-ACE2, Abcam; anti-Actin, Abcam) diluted in PBST containing 1% BSA (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at 4°C overnight with primary antibodies including: NeuN (1:500, mouse, Millipore, Cat#:MAB377; 1:500, rabbit, Abcam, Cat#: ab104225), MBP (1:300 ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was incubated for 22-24 h at 4 °C in a solution of primary antibody (1:2000 rabbit polyclonal to c-Fos, Abcam, Cambridge, MA) with 5% normal goat serum (Abcam ...
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bioRxiv - Immunology 2024Quote: ... and incubated overnight at 4°C with the anti-F4/80 (#ab6640, 1:50) and/or anti-iNOS (#ab3523, 1:20) antibodies from Abcam (Cambridge, UK), and/or anti-AMPK alpha (Phospho Thr172 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with primary antibodies at the following dilutions: rabbit anti-MAP2 (ab254264 Abcam, 1:2000), mouse anti-βTUBULIN III (TUJ1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated overnight at 4°C with anti-TOMM20 (1:200 dilution) and anti-cytochrome C (1:200 dilution) primary antibodies (Abcam, Waltham, MA). Thereafter ...
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bioRxiv - Neuroscience 2023Quote: ... The embedding blocks were cut on a cryostat into 20 μm thick sections and blocked for 30 min with 4% BSA and then incubated for 1 hour with the following primary antibodies: polyclonal rabbit anti-RFP (1:200, Abcam, Cambridge, UK).
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with primary antibodies diluted in blocking buffer overnight at 4°C - Mouse Anti-Human TRA1-81 (1:75, Abcam, AB16289#20), Rabbit Anti-Human OCT4 (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... LiCor) for 1 hour at room temperature and probed overnight at 4℃ with a monoclonal rabbit anti-HTT antibody (Abcam: EPR5526; Ab_109115) diluted 1:1,000 in blocking buffer plus 0.2% tween ...
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bioRxiv - Genetics 2023Quote: Slides were incubated overnight at 4°C with the following antibodies diluted in PBS: rabbit anti-SYCP3 (ab15093, Abcam, 1:200 dilution), rabbit anti-SYCP1 (ab15087 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 48-72 hours at 4°C in primary antibodies: anti-Choline acetyltransferase 1:300 (Abcam catalog #: ab178850; RRID:AB_2721842), anti-Tyrosine hydroxylase 1:300 (Abcam catalog # ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated at 4°C overnight with a monoclonal antibody to human cytokeratin antigen (Abcam, Cat #ab53280, at 1:250), or monoclonal antibody to human POSTN (R&D Systems ...
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bioRxiv - Pathology 2023Quote: ... membranes were incubated overnight at 4 °C with agitation with primary antibodies against acetylcholinesterase (68 kDa, 1:500, ab97299; Abcam, Cambridge, UK), butyrylcholinesterase (75 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in a humid chamber overnight at 4°C in primary antibody: anti-GFAP (Abcam [ab4674], 1:200 dilution), anti-Iba1 (Abcam [ab178846] ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS) for 1h at room temperature and then incubated overnight at 4°C with primary antibodies: anti-melanopsin (Abcam, 1:200), anti-GFP (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were fixed in 4% paraformaldehyde for 15 min and stained with anti-γ-H2AX (CST,9718) and chicken anti-GFP (Abcam, #ab13970) primary antibodies in 1:400 and 1:600 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed with 4% PFA (for 10 min) and stained with an anti-sarcomeric alpha actinin antibody [EA-53] (1:500; Abcam, cat#ab9465) followed by a phalloidin Texas Red- conjugated antibody (1:800 ...
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bioRxiv - Cancer Biology 2023Quote: ... end-stage bulk tumor and 4- to 15-weeks control HDTVi liver protein lysates (50 μg) were analyzed using Fluorometric Caspase-1 Assay Kit (Abcam, cat.no. ab273268) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were incubated ON at 4°C with the following primary antibodies in blocking solution: rabbit-anti-DYRK1A (Abcam, 1:100), mouse anti-DYRK1A (Abnova ...
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bioRxiv - Biochemistry 2024Quote: ... the membrane was incubated (overnight, 4 °C) with the anti-α-syn primary antibody MJFR1 (diluted 1:1000 in PBS-Tween, Abcam, UK). Following rigorous washing with PBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated overnight at 4 °C with mouse Anti-GABAAR γ2 primary antibody (1:500; catalog no. ab307231, Abcam, United Kingdom) in a permeable buffer containing 2% donkey serum ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated on an orbital shaker for 24 hours at room temperature + 24 hours at 4 °C in polyclonal rabbit anti-mCherry (1:30 000, Abcam, cat. ab167453) or polyclonal chicken anti-EYFP (1:100 000) ...
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bioRxiv - Biophysics 2024Quote: ... and then were blocked with the 10% normal goat serum for 45 minutes at room temperature before incubating with the primary antibodies (anti-γH2AX: Beyotime, no. C2035S-4; anti-pSTING: Cell Signaling Technology, no. 40818S; anti-cytochrome c and anti-complex V: Abcam, no ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed twice with PBS before incubation overnight at 4°C in primary mouse IgG2b anti-NeuN (1:500, Abcam cat# ab104224). Sections were washed twice the following day with 1X PBS followed by incubation in goat anti-IgG2b AlexaFlour 647 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated O/N at 4°C with the following primary antibodies diluted in the blocking solution: rabbit polyclonal anti-GFAP (1:1000; abcam, cat# ab7260), rabbit monoclonal [EPR16588] anti-Iba-1 (1:1000 ...