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Citations for abcam :
2801 - 2850 of 3162 citations for 2 4 Methoxyethoxy 3 methyl 2 pyridinyl methylthio benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used for cell staining overnight at 4°C: anti-alpha smooth muscle actin [1A4] antibody (Abcam) and isotype control mouse IgG2a (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 1 h at RT and incubated with primary antibodies for at least 1 h at RT or overnight at 4°C (p300, Abcam, ab275388; CBP, Abcam, 253202 ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 1 h at RT and incubated with primary antibodies for at least 1 h at RT or overnight at 4°C (p300, Abcam, ab275388 ...
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bioRxiv - Neuroscience 2021Quote: ... following which sections were blocked in 3% normal horse serum (NHS) for 30min before overnight incubation with Tyrosine hydroxylase (TH) (Abcam, ab112, 1:750) or IBA-1 (Wako ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... The same procedure was used for flat-mounts but primary incubation was for 3 days and secondary incubation for 1 day using anti-GFP 1:500 (Abcam, Cambridge, UK; Chk).
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bioRxiv - Bioengineering 2023Quote: ... The slices were then incubated overnight at 4°C with primary antibody solutions (supplemented with 3% (v/v) Triton X-100 in 1× PBS) to target astrocytes (GFAP) and neuronal nuclei (NeuN) (Abcam Inc., Cambridge, UK) (Table 1) ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in 1x PBS for 10 minutes then incubated in blocking solution containing AlexaFlour488 conjugated secondary antibodies (Abcam, ab150077; 1:1000) for 2-4 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for western blotting at the indicated dilutions: anti-CD63 (MBL, D263-3, 1:5000; Abcam, ab134045, 1:2000), anti–Flotilin-1 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... gravid worms were placed in 1 drop of ddH2O containing 3% (v/v) pelleted OP50 and 100 μM bafilomycin A1 (Abcam, dissolved in DMSO) for 16 h at 20 ℃ ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were incubated for 3 h at room temperature with a mix of secondary antibodies (ThermoFisher A-11039 or Abcam ab150158, 1:400) that had been previously centrifuged at 20000g for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... carried out overnight on a rotating wheel at 4 °C in the presence of 20 μg of anti-V5 antibody (Abcam, ab9116). 2.5% of the input sample was collected just before addition of the antibody and stored at −20 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated at 4°C with one of the following mouse monoclonal primary antibodies: anti-heavy chain cardiac myosin (Abcam, #ab50967), anti-α-actinin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were blocked with 5% donkey serum and incubated overnight at 4°C against MMP13 (1:100, Abcam ab39012, Cambridge, UK) and for 2 hours at room temperature with a Cy3-conjugated secondary (1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were incubated in primary antibodies diluted in blocking solution overnight at 4°C as follows: rabbit anti-CASP3 (1:500, Abcam, ab49822); mouse anti-COL2A1 (1:400 ...
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bioRxiv - Developmental Biology 2020Quote: ... at 4 °C overnight followed by staining with a secondary goat-anti-rabbit antibody coupled with Alexa 568 (ab175471, Abcam, US).
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bioRxiv - Developmental Biology 2020Quote: ... tissue sections were blocked with 2% Bovine Serum Albumin in PBS for 1 h and then incubated overnight at 4 °C with the following antibodies: ISLET-1 (1:200, abcam, ab109517), HES1 (1:200 ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21: ab88572, Abcam; anti-GFP: ab290, Abcam). Paired-end sequencing was performed on a NovaSeq6000 platform (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in 4% NGS containing an antibody targeting αsyn phosphorylated at serine 129 (anti-pSer129, rabbit monoclonal, 1:600, Abcam #51253). Subsequently ...
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bioRxiv - Bioengineering 2022Quote: ... The following primary antibodies were used at a 1:200 dilution (as recommended by the manufacturer) and incubated overnight at 4°C: anti-αSMA (Abcam ab5694) or anti-Rac2 (Fischer Scientific ...
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bioRxiv - Genetics 2020Quote: ... and then incubated in blocking solution overnight at 4°C with the following antibodies: rabbit monoclonal anti-ATP6V1A (1:1000)(Abcam EPR19270), rabbit polyclonal anti-GNE (1:1000)(Proteintech 25079-1-AP) ...
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bioRxiv - Pathology 2021Quote: ... The membrane was then probed overnight at 4°C with primary antibodies (P105/p50, 1:1000, Abcam ab32360; Actin, 1:1000, Abcam ab3280) in Odyssey Blocking Buffer and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections from animals not injected with FITC-Dextran were next incubated under stirring for 48 hours at 4°C in PB containing rabbit polyclonal anti-collagen IV antibody (Abcam, Ab6586) at a 1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were blocked in TBST buffer containing 5% nonfat milk and then incubated overnight at 4°C with the primary rabbit polyclonal anti-Tip60 (1:1000, abcam, #ab23886), rabbit polyclonal anti-Rpd3 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated with 10% BSA in TBST for 1 hour at room temperature followed by incubation at 4°C overnight with anti-Iba1 antibody (goat, 1:500, Abcam #Ab5076) and anti-phospho-Akt-Ser473 antibody (rabbit ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated overnight at 4°C in primary antibodies: biotinylated goat anti-GFP (1:400; Abcam, catalog #ab6658), Mouse anti-RFP (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were resolved by SDS-PAGE using a 4-15% gradient gel and analyzed by Western blot using the following primary antibodies: rabbit anti-GFP (Abcam Ab290), rabbit anti-Cx34.1 3A4-conjugated-680LT ...
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bioRxiv - Microbiology 2021Quote: ... then incubated overnight at 4°C with the primary antibody mouse anti-SA Hla [8B7] -N-terminal (Abcam, Cambridge, United Kingdom) diluted 1:2500 in the blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887] ...
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bioRxiv - Cell Biology 2021Quote: ... Afterwards membranes were blocked for 30 min with 5% Nutrilon (Nutricia) in PBS and incubated overnight at 4°C with primary antibodies H3 (Abcam 1791), H4 (Merck Millipore 04-858) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked overnight with 5% BSA in PBST at 4 °C and then incubated with 1:50 RAD51 primary antibody (sc-8349, Santa Cruz or ab63801, Abcam) in 2.5% BSA in PBST for 1 hour at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... cells were incubated overnight at 4 °C with recombinant anti-vimentin antibody conjugated with Alexa Fluor® 568 (ab202504, Abcam®) at a 1/1000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4 °C in PBST with primary antibodies including: rabbit anti-c-Fos (1:2000, Abcam, ab190289), goat anti-c-Fos (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... cut based on the molecular weights indicated by the prestained marker and then incubated overnight on a shaker at 4 oC with rabbit anti-human STEAP3 (1:1000) (abcam, 151566), mouse anti-human p53 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were blocked for 1 hour at room temperature with 5% fat-free milk or milk-free blocking buffer (for analysis of phosphorylated proteins) and incubated overnight at 4°C with primary antibodies for p-GCN2 (Abcam, ab75836), p-eIF2α (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... slides were thoroughly washed using PBS 1X/Triton- X 0.1% and incubated overnight at 4°C with the respective antibodies anti-mouse Alexa-Fluor® 594 (abcam, ab150108), anti-sheep Alexa-Fluor® 488 (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2022Quote: ... and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2, 1:1000, Abcam; rabbit anti-FOXP2, 1:1000, Abcam; mouse anti-FOXP2 ...
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bioRxiv - Developmental Biology 2022Quote: ... for 1 hr at RT then stained O/N at 4° C using the following primary antibodies diluted in blocking buffer: Chromogranin A (1:200, Abcam, ab15160), Glucagon (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... for 2 h and then incubated overnight at 4°C with primary antibody to measure APOA1 (1:10 000, cat# ab52945, RRID:AB_2056661, Abcam, Cambridge, United Kingdom). Afterward ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated with the following antibodies overnight at 4°C: rabbit polyclonal α-smooth muscle actin (SMA, 1:200; Abcam) and biotinylated DBA (1:100 ...
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bioRxiv - Immunology 2022Quote: ... and CTLA-4 Ig (Abatacept) were generated using the APC Conjugation Kit - Lightning-Link® according to manufacturer’s instructions (Abcam, ab201807). APC conjugated anti-PD-L1 antibodies were procured from Biolegend (clones MIH1 ...
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bioRxiv - Neuroscience 2022Quote: ... and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2, 1:1000, Abcam; rabbit anti-FOXP2 ...
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bioRxiv - Neuroscience 2020Quote: ... 115-007-003) was used for antigen blocking before overnight incubation at 4 °C with rhodopsin 4D2 (1:500, Abcam, ab98887). Slides were washed and incubated with goat anti-mouse 488 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... endogenous human NPC1 and HSP90 were purified using immobilized Recombinant Protein G Resin (Generon) and 4 µg of specific antibodies against NPC1 (Abcam, ab108921) or HSP90 (Enzo Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... The rest of the chromatin was incubated overnight at 4°C with 1 μg of polyclonal anti-GFP antibody (Abcam ChIP grade, Abcam 290) or ...
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bioRxiv - Neuroscience 2021Quote: ... sections were again triple washed with PBST and then incubated overnight at 4°C with a primary rabbit anti-GFP/YFP antibody (1:1000, Abcam, ab6556). After this ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 hr at RT and incubated in PBST overnight at 4°C with following primary antibodies: chicken anti-GFP (1:1000, Abcam ab13970), chicken anti-MAP2 (1:10000 ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked for 1 hr at RT in 3% BSA PBS 0.1% Tween-20 (PBS-T) and then incubated overnight at 4°C with primary antibody (rabbit anti-RGS9, 1:500 in blocking solution; Abcam, UK). After washing three times in PBS-T ...
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bioRxiv - Bioengineering 2020Quote: ... Incubation with primary antibody was at 4°C overnight with either anti-CD31 diluted 1:100 (ab28364, Abcam, Cambridge, MA, USA) or anti-active caspase3 diluted 1:100 (ab2302 ...