Labshake search
Citations for abcam :
2951 - 3000 of 3153 citations for Human Caveolin 2 CAV2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred to 0.2 um nitrocellulose or PVDF membranes and detected with primary antibodies targeting LRRK2 (ab133474, Abcam, RRID: AB_2713963, 1:500), pS935 LRRK2 (ab133450 ...
-
bioRxiv - Plant Biology 2021Quote: ... For each ChIP/re-ChIP assay, 20 μg DNA/protein complexes were immunoprecipitated with anti-H3K27me3 (Milipore, Cat No 07-449) and anti-H3K18ac (Abcam, Cat No ab1191) antibodies using the Re-ChIP-IT kit (Active Motif ...
-
bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were then processed through an ELISA based kit for detecting p65 (NF-κB p65 Transcription Factor Assay Kit, abcam, Cambridge, MA, #ab133112). Absorbance at 450 nm was read with SpectraMax iD3 plate reader (Molecular Devices ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
-
bioRxiv - Neuroscience 2021Quote: ... Co-labeling for astrocytes was performed using the same anti-glial fibrillary acidic protein antibody described above (GFAP; Abcam, ab53554; 1:250 dilution). Secondary antibody incubation labeling GFAP positive cells was performed for 1 hour at room temperature using Alexa Fluor 488-conjugated Donkey anti-Goat (ThermoFisher ...
-
bioRxiv - Physiology 2021Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:250 dilution), including V-ATP5A (55kDa) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were then probed to determine protein levels using the following antibodies diluted in 5% non-fat milk in 1x TBST: matrix metalloproteinase 9 (MMP9) (Abcam ab38898, 1:1000) and MMP2 (Abcam ab92536 ...
-
bioRxiv - Genetics 2019Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane and probed with well-characterized monoclonal antibody of anti-Fibrillin 1 (Abcam ab124334, Cambridge, UK).
-
bioRxiv - Bioengineering 2019Quote: ... Tangential cortical sections of 30 μm-thickness were sliced using a cryostat and directly mounted on gelatin-subbed glass slides for immunohistological staining of GFAP (glial fibrillary acidic protein, Abcam, Cambridge, MA, USA) for astrocytes ...
-
bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
-
bioRxiv - Microbiology 2020Quote: ... the fixed cells were labeled with primary antibodies including goat anti-major outer-membrane protein (MOMP; Meridian, Memphis, TN) and mouse or rabbit anti-six histidine tag (Genscript, Nanjing, China and Abcam, Cambridge, UK, respectively). For experiments using CRISPR interference ...
-
bioRxiv - Plant Biology 2024Quote: ... Protein expression of rSPL9-GFP was verified by SDS-PAGE and immunoblotting using an anti-GFP antibody (Abcam, Cat# ab290, 1:10,000 dilution) and HRP-conjugated secondary antibody (Promega ...
-
bioRxiv - Physiology 2024Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:250 dilution), including V-ATP5A (55kDa) ...
-
Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... while sections of caspase and protein S staining were incubated with goat anti-rabbit IgG secondary antibody conjugated with HRP (Abcam ab6721, 1:1,000). ImmPACT DAB Peroxidase (HRP ...
-
bioRxiv - Developmental Biology 2023Quote: Expression of Br-Z1 and Blimp-1 proteins was inferred from staining knock-in strains with chicken anti-GFP (Abcam Ab13970 1:500) and an anti-chicken secondary antibody (Invitrogen A-21437 1:1000).
-
bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
-
bioRxiv - Cell Biology 2024Quote: ... Transfer quality and total protein were assessed by Ponceau-S staining before the membranes were probed for Myc-tag (Abcam, #ab9106, 1:1000). Fluorophore-conjugated secondary antibodies were used for detection using the Licor Odyssey CLx system (anti-rabbit IgG ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were fixed in 2% formaldehyde in PBS for 10 min at room temperature and stained with Phalloidin-iFluor 594 (abcam, ab176757) for 30 minutes at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... the chromatin fraction was solubilized in EBC-150 buffer with 500 U/mL Benzonase and 2 ug of antibody directed to endogenous RNAPII-S2 (Abcam; ab5095) or RNAPII-S5 (Abcam ...
-
bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
-
bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with secondary antibody for 2 h at room temperature before DAB reaction using the DAB detection kit (Abcam #ab64238). Slides were counterstained with hematoxylin to stain nuclei ...
-
bioRxiv - Developmental Biology 2020Quote: ... Slides were washed in PBST as above to remove the excess of the secondary antibody and then one drop of Fluoroshield Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, #ab104139) was added on the immunostained samples.
-
bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
-
bioRxiv - Neuroscience 2021Quote: ... end stage high- titer viral aliquots or 2) from cell culture media used for rabies virus rescue after PEG- based precipitation (Abcam, ab102538) and quantified using the High Sensitivity RNA ScreenTape assay (Agilent ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were again washed thrice in 0.1 M PB and then incubated with the secondary antibody for 2 h (goat anti-chicken-IgY conjugated to Alexa Fluor 594, Abcam, ab150172). Following the incubation with the secondary antibody ...
-
bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076; donkey anti-goat IgH&L Alexa488, Abcam, ab150129). Tissues were washed in 1% Triton X-100/PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... for 10 min and then incubated with the following secondary antibody for 2 hrs: goat anti-mouse IRDYe® 680 conjugated antibody (1:10,000; abcam; ab216776). The membrane was washed again with PBST three times for 10 min and imaged on the Odyssey Li-Cor.
-
bioRxiv - Neuroscience 2020Quote: ... Each section was then washed three times in PB for 5 minutes each and incubated for 2 hours at 4°C in a 1:500 dilution of Alexa Fluor 488 goat anti-rabbit secondary antibody (Abcam, Ab150077). From this step on ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times with PB and then incubated with the secondary antibody for 2 hours (goat anti chicken Alexa Fluor 594, abcam, ab150172). After incubation with the secondary antibody ...
-
bioRxiv - Neuroscience 2020Quote: ... Tissue was then incubated for 2 hours at room temperature in a PBTGS secondary antibody solution including goat anti-mouse Alexa568 (1:200; Abcam [ab175473]), goat anti-rabbit Cy2 (1:200 ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by a 2 hr incubation with a mixture of the secondary antibody (goat anti-mouse Alexa Fluor® 488–conjugated IgG, ab150113 Abcam) and Alexa Fluor 568 conjugated Phalloidin (A12380 ...
-
bioRxiv - Cell Biology 2021Quote: ... for 2 hours at room temperature (Chicken anti-GFP, Aves cat#GFP-1020, RRID:AB_10000240; Rabbit anti-NUP153, Abcam cat#ab84872, RRID:AB_1859766), followed by washing 3x 5 min in 1x PBS ...
-
bioRxiv - Microbiology 2021Quote: ... Platelets were then permeabilized with 0.25% saponin for 30 min and stained using recombinant anti-Bcl-2 antibodies (AF647, Abcam, Berlin, Germany) for 30 min before being measured by flow cytometry ...
-
bioRxiv - Microbiology 2021Quote: ... Gels were either stained with Coomassie brilliant blue or subjected to Western blot using appropriate antibodies: rabbit polyclonal anti-SARS-CoV-2 S antibody (Abcam ab272504) or rabbit polyclonal anti-SARS-CoV/CoV2 M antibody (Novus Biologicals NB100-56569).
-
bioRxiv - Molecular Biology 2021Quote: ... cells were permeabilized using 0.1% Triton-X and incubated for 2 hours at room temperature with mouse monoclonal antibody against sarcomeric α-actinin (1:100; Abcam), followed by an incubation of 1 hour with the secondary antibody conjugated to Alexa Fluor-488 (Life Technologies) ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with filipin III (1:100) in PBS for 2 h as described in the manufacturer’s protocol of the cholesterol assay kit (Abcam Biochemicals, ab133116). Filipin III was also added in all following incubation steps with the primary and secondary antibody to enable proper staining ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
-
bioRxiv - Cell Biology 2021Quote: Total intracellular ROS contents of cultured HCs were measured using the fluorescent probe 2’,7’-dichlorofluorescin diacetate (DCFDA) (ab113851, Abcam, UK) (Lv et al. ...
-
bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit ...
-
bioRxiv - Microbiology 2022Quote: ... low or no ACE2 were resuspended in FACS buffer (PBS+2% BSA) and incubated for 1 h with 1:500 rabbit anti-ACE2 antibody (Abcam ab272500). Cells were washed with FACS buffer and labeled for 1 h with 1:3000 Alexa-Fluor-488 goat anti-rabbit IgG H&L (Abcam ab150077) ...
-
bioRxiv - Cell Biology 2022Quote: ... Epitope detection was conducted with a primary monoclonal mouse antibody against NONO at 1:500 dilution in PBST and a polyclonal rabbit antibody against RNA Pol II phosphorated at Serine 2 (Abcam, ab5095) at 1:500 dilution ...
-
bioRxiv - Bioengineering 2022Quote: ... Samples were washed in distilled water and stained for 15 min in 2% w/v Alizarin Red (ab146374, Abcam, Cambridge, UK) in distilled water at a pH of ~4.2 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 0.7% Triton X-100 in 1X PBS) for 2 hours at room temperature and incubated in blocking solution with anti-GFP antibody (1:500, Abcam, ab6658) overnight at 4 ºC ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 1 h with primary antibodies (dilution in 10% goat serum in PBS) to p-Pol 2(S5) (1:1000 dilution; Abcam, #ab5408) and H3K27ac (1:2000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF-14 (rabbit monoclonal MJFR-14-6-4-2, #ab209538, Abcam, 1:25,000) and mouse monoclonal pS129-α-syn (11A5 ...
-
bioRxiv - Cell Biology 2021Quote: ... and reacted with an antibody to synaptic vesicle 2 (SV2 1:50; Developmental Studies Hybridoma Bank Ab2315387) and neurofilament 200 (NF200 1:500, Abcam Ab72996) for 24 hours at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized with 0.5% TBS-T and 5% BSA and incubated overnight at 4°C with primary antibodies anti-MAP-2 (1:500, Abcam; ab32454) and anti-GFAP (1:500 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sections were rinsed with PBS and blocked for 2 hours at room temperature using 10% normal donkey serum (NDS; Abcam, #ab7475) prepared in 1% BSA in PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... and treated with blocking solution (TBS containing 5% normal goat serum and 0.2% Triton X-100) for 2 hr and incubated overnight at 4°C with chicken anti-GFP monoclonal antibody (1:500; abcam; ab13970). After washing three times with TBST (TBS containing 0.2% Triton X-100) ...
-
bioRxiv - Molecular Biology 2019Quote: ... PVDF membranes were blocked with 5% nonfat milk in TBST for 2 hours at room temperature and incubated with primary antibodies specific for Osx (1:1000, Abcam, UK), Runx2 (1:1000 ...