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Citations for abcam :
2801 - 2850 of 3153 citations for Human Caveolin 2 CAV2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... cell lysates were collected in parallel with the supernatants from the same wells and then tested for levels of a housekeeping protein beta-actin (Ab8227, Abcam, RRID: AB_2305186) by western blot ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde and processed for immunofluorescence analysis using GAP SH3 Binding Protein 1 (G3BP1) antibody (green; Abcam, Cambridge, MA) and fluorophore-conjugated secondary antibody ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Astrogliosis was visualized after staining with rabbit Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody at a 1:1000 dilution (Abcam, Cambridge, MA). All samples were analyzed on an inverted epifluorescent microscope (Olympus IX71) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated over night with primary antibody rabbit-anti-Ca2+/calmodulin-dependent protein kinase II (1:200, #PA5-38239, Thermo Fisher, MA, USA; 1:500, #ab52476, Abcam, UK), rabbit-anti-parvalbumin (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... and analyzed by western blot using anti-HBP1 (11746-1-AP, Protein Tech Group, 1:500) or anti-ZMYND19 antibody (ab86555, Abcam, 1:500).
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Biophysics 2021Quote: ... threonine and tyrosine phosphorylation of the wt and mutant T654A proteins was detected by western blotting using rabbit anti-pT654 (ab75986; Abcam, Cambridge, UK) and rabbit anti-pY1086 antibody (#4407 ...
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bioRxiv - Cell Biology 2022Quote: ... The relative amount of each of these muscle proteins in each lane was normalized to the amount of Histone H3 detected using anti-Histone H3 (abcam, cat. ab1791) at 1:40,000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 1 μg of a specific α2 AMPK antibody (ab3760, Abcam, Cambridge, MA) coupled with protein G-Sepharose beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was clarified by centrifugation at 21,130 x g for 15 minutes before analysis of protein levels in the supernatant by immunoblotting using antibodies including anti-PKA pRIIα (Abcam, RRID: AB_779040), anti-GFP (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was used for indirect immunofluorescence microscopy at a dilution of 1:300 and mouse monoclonal antibody against nuclear pore complex proteins (Abcam, Cambridge, MA) was used for indirect immunofluorescence microscopy at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Electroporated cells were seeded in StemMACS iPS-Brew XF with Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM, Abcam, Cambridge, UK) and 1x CloneR (Stemcell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant calibrated into the same protein concentration was used for the measurement of oxytocin levels using an ELISA kit (Abcam, lot. 133050), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: Nuclear protein isolations from frozen muscle tissue were performed using a commercial kit (Nuclear Extraction Kit, catalog# ab113474; Abcam; Waltham, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... They were then washed with PBS and incubated with 1° antibodies (α-NDUFV2, 15301-1-AP, Protein tech, α-tubulin, Abcam, ab179513) in 5% milk 0.1% Tween 20 ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of protein of EV or lysate samples were then subjected to western blot analysis for ALIX (ab186429; Abcam, Cambridge, UK) at 1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... end-stage bulk tumor and 4- to 15-weeks control HDTVi liver protein lysates (50 μg) were analyzed using Fluorometric Caspase-1 Assay Kit (Abcam, cat.no. ab273268) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Detection of thiophosphate ester tagged proteins from the in vitro kinase assay was done with a specific anti-thiophosphate ester antibody (#ab92570 Abcam 1:10000). Antibody against total CDC-42 was use for the loading control (#sc-390210 ...
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bioRxiv - Systems Biology 2024Quote: ... was conducted to normalize protein concentrations between all samples and hexokinase activity was measured according to the manufacturer’s protocol (Abcam, Cambridge, MA; ab136957), using 10 μg of protein per condition ...
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bioRxiv - Microbiology 2024Quote: ... and a rabbit antibody recognizing cellular proteins or epitope tags (anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-TGN46, Abcam, ab50595, 1:600; anti-GM130, Abcam, ab52649 ...
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bioRxiv - Genomics 2024Quote: ... we extracted the total protein from the mutant cells and conducted western immunoblotting using a recombinant anti-HA tag antibody (Abcam, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... and labelled with primary antibody (1:1000 diluted in blocking buffer made up by 1% non-fat dry milk in calcium and magnesium free PBS) to tyrosinated tubulin [YL1/2] (rat monoclonal, ab6160, Abcam) for 45 min at room temperature which is followed by PBS washes repeated three times and 10 min of blocking ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were washed 3 times in PBS and then incubated for 2 h in secondary antibody (1:200 dilution AlexaFluor647 conjugated donkey anti-goat (ab150135, Abcam) and 1:200 dilution FITC-conjugated rabbit anti-rat (ab6730 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 20% milk in PBST for 1h following primary antibody incubation (COX-2: Abcam, Cat# ab15191, 1:1000 dilution, 1h at room temperature in 1% BSA; CYP2E1: Abcam, Cat# ab28146 ...
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bioRxiv - Systems Biology 2022Quote: ... and the supernatant containing chromatin was incubated with 20 μg of antibody (INO80 antibodies, abcam cat # ab105451, lot GR3206182-2) overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and 2% NGS (block) for 1h at room temperature before incubation in primary antibodies (rabbit anti-Synapsin I, ab64581, Abcam; guinea pig anti-GLT-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2, ab18465, Abcam; 1:1000 Rabbit anti-GABAalpha6 ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...
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bioRxiv - Immunology 2020Quote: ... 6 µg of pre-cleared chromatin was immunoprecipitated with 2 µg/IP anti-Histone H3K27Ac (Abcam Cat#ab4729; RRID: AB_2118291), 4 µg/IP of anti-LXRα/β (provided by Knut Steffenssen ...
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bioRxiv - Molecular Biology 2021Quote: ... blocked in 2% normal donkey serum/1% BSA in PBS and incubated overnight with guinea pig anti-insulin (1:500, Abcam) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with primary antibodies against Rad53 (1:4000; rabbit, ab104332, Cell Signalling) or Tubulin (1:2000; rat, YL1/2, Abcam) diluted in TBS-T containing 1% BSA for 1 h 30 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were blocked with PBST (PBS+ 0.05% Tween 20) + 2% BSA for 15 min and incubated 3h at RT with anti-GFP antibody (1:100, 6556 Abcam) in PBST/BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies were incubated overnight at 4°C in 2% milk TBST: anti-CENP-F(Ab5) (1/1500, Rabbit, Abcam), anti-CENP-F(A301-611A ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076 ...
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bioRxiv - Genomics 2019Quote: ... Immunoprecipitation was performed overnight at 4°C with rotation using 1-2 × 106 cell equivalents per immunoprecipitation using antibodies (5 µg) against H3K4me1 (Abcam), H3K27ac (Abcam) ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed by incubation of 2 ml diluted chromatin (equivalent to 5×106 input cells) with antibodies for H3K4me1 (3 µg ab195391, lot: GR304893-2; AbCam), H3K4me3 (1 µl 07-473 ...
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bioRxiv - Immunology 2019Quote: ... Sheared chromatin was then incubated with primary antibodies (AR [06-680, Millipore], H3K4me3 [ab8580, Abcam], phospho-Ser5 RNA polymerase 2 [ab5131, Abcam] ...
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bioRxiv - Developmental Biology 2019Quote: ... rat anti-Stab2 1:100 (clone #34-2, MBL-D317-3) and rabbit anti-CD44 1:200 (polyclonal ab157107, Abcam).
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bioRxiv - Immunology 2021Quote: ... plus either rabbit polyclonal anti-SARS-CoV-2 Spike/RBD (Sino, 40592-T62) or rabbit polyclonal anti-CD11b (Abcam, ab52478) for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with non-fat dry milk and incubated overnight with primary antibodies at 4°C (Abcam polyclonal rabbit IgG: Anti-MMP2 – ab37150, Anti-MMP3 – ab53015, Anti-MMP9 – ab38898, Anti-COX-2 – aDb15191 and Abcam mouse monoclonal anti-beta III Tubulin IgG2a – ab78078 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C for 24 hours in 2% normal goat serum together with rabbit anti-Nrf2 at 1:500 (Abcam). Slides were then washed and incubated in the secondary antibody (1:1500 ...
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bioRxiv - Neuroscience 2021Quote: ... Lysis buffer (930 µl) and 2 µg of either JADE1 antisera or anti-0N tau antisera (EPR21726, Abcam, Cambridge, MA) were combined and incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Thermo Fisher Scientific Baltics UAB complies with Quality System Standards ISO 9001 and ISO: 13485) per sample with 2 pg anti-BrdU monoclonal antibody (cat# ab6326, Abcam, Cambridge, UK). Beads were washed four times with 300 pl of PBST wash buffer including SUPERase In RNase Inhibitor (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...