Labshake search
Citations for abcam :
2651 - 2700 of 2723 citations for Human Complement C3 Convertase C3c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: GAPDH activity was measured in the presence and absence of GAs using Glyceraldehyde 3 Phosphate Dehydrogenase Activity Colorimetric Assay Kit (ab204732, Abcam, Cambridge, MA) as per the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentrations of NAD(H) (total NAD+/NADH) and NADH only were measured by the NAD+/NADH assay kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were stained using the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit (Abcam, United States; Cat. no. ab80436). The enzyme expression was visualized with the mouse monoclonal antibody anti-SOD2 (Santa Cruz ...
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bioRxiv - Immunology 2020Quote: ... Followed by capture of SARS-CoV-2 receptor binding domain which was biotinylated using the lightning-link type-A biotinylation kit(Expedeon/Abcam, 370-0005) for 180s at 10ul/min ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Absolute quantification of sialic acid in different glycoconjugate fractions was performed using the Sialic Acid (NANA) Assay Kit (Ab83375, Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... equal number of cells were lysed using assay buffer provided in the alpha KG assay kit (abcam, ab83431, lot no. GR3410863-1). The lysates were then deproteinized using perchloric acid and nutralized using 2M KOH as per manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Cell cycle analysis was performed by propidium iodide (PI) staining following the protocols from the propidium iodide flow cytometry kit (Abcam, Cat. ab139418). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant calibrated into the same protein concentration was used for the measurement of oxytocin levels using an ELISA kit (Abcam, lot. 133050), according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... Total protein levels were measured by BCA assay (Pierce) and a 1:00 dilution was used for quantifying α-synuclein using the mouse α-synuclein ELISA kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... 100 ug of anti-SARS-CoV-2/S2 (1A9) antibody was conjugated to A647 using the Lightning-Link Conjugation Kit (Abcam, Cat# ab269823) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... were used to quantify the ratio of reduced (GSH) to oxidized (GSSG) circulating glutathione with a commercial ELISA kit (Abcam; Cambridge, UK) as an indicator of oxidative stress levels and redox imbalance.33
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bioRxiv - Plant Biology 2023Quote: ... Two microliter aliquots of the supernatants were transferred to wells of a 96-well plate prefilled with 98μl of assay buffer of the ammonia assay kit from AbCam (AbCam Cat # ab102509), and ammonia levels were quantified according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... serotonin and brain derived neurotrophic factor using commercially available Enzyme linked immunosorbent assay kits according to the instructions of the manufacturer (ABCAM, Cambridge UK)
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bioRxiv - Genomics 2023Quote: The cholesterol assay was carried out according to the manufacturer protocol using the Cholesterol/Cholesterol Ester Assay Kit (ab65359, Abcam, Cambridge, UK) and normalized to total protein content as determined by Bradford Assay.
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bioRxiv - Biochemistry 2023Quote: Right Ventricular Mitochondrial Enrichments: RV mitochondrial enrichments were isolated from the same PAB and control piglets with a mitochondrial isolation kit (Abcam, Cambridge, MA) as previously described(10 ...
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bioRxiv - Bioengineering 2023Quote: ... was dissected from the constructs at the end of maturation time (day 15) and tested via a colorimetric CK Activity Assay Kit (Ab155901, Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... regularly spaced (500 µm) along the rostrocaudal axis were stained with a Prussian blue and nuclear fast red stain kit (Abcam, Cambridge, UK) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: RCC243 cells were treated with 5 μM MS023 for the indicated time periods and then cells were trypsinized and stained using the Propidium Iodide Flow Cytometry Kit (Abcam, #ab139418, Abcam) based on the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... None of the cultures or treatments showed signs of cell cytotoxicity using the using the LDH-Cytotoxicity Assay Kit II (Abcam, cat. #ab65393) to analyze 50 ul fresh culture media (not shown) ...
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bioRxiv - Neuroscience 2024Quote: Choline and acetylcholine were quantified in rat cortical tissue from all experimental groups using a choline/acetylcholine assay kit (ab65345, Abcam, Cambrige, UK). 10 mg of cortical fresh tissue were harvested ...
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bioRxiv - Physiology 2024Quote: Mitochondrial membrane potential was measured in γY4KD cells with Pparγ silenced with siRNA and their scrambled control (Scrl) using Mitochondrial Membrane Potential Assay kit (Abcam, Cat# ab113852). Cells were plated on collagen coated 24-well plate at density of 50,000 cells/well (5 well/per group) ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Cancer Biology 2023Quote: ... end-stage bulk tumor and 4- to 15-weeks control HDTVi liver protein lysates (50 μg) were analyzed using Fluorometric Caspase-1 Assay Kit (Abcam, cat.no. ab273268) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: D2HG levels were measured in adherent cultures of 1 x 107 chondrosarcoma cells treated with increasing concentrations of Enasidenib for 48 hours using the D2HG colorimetric Assay Kit (Abcam, Cambridge, UK) following the manufactureŕs recommendations.
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bioRxiv - Synthetic Biology 2023Quote: ... listed in Supplemental Table 2 were used to quantify TrxB expression levels with the GFP ELISA kit (Abcam, Cambridge, UK, Cat# ab171581) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... MockIP and WCL were aliquoted into wells in a 96-well plate and assayed using a fluorometric Cathepsin D activity assay kit (Abcam. Cat# Ab65302) that is based on quantifying the hydrolysis of the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2 ...
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bioRxiv - Cancer Biology 2024Quote: ... the adherent monolayers were incubated with 100 µl/well cell lysis buffer from the cAMP Direct Immunoassays Kit (Fluorometric, ab138880, Abcam, Cambridge, UK), while the gliomaspheres were collected ...
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bioRxiv - Pathology 2024Quote: ... Masson’s trichrome staining was performed to assess endometrial fibrosis in sections within a 20 μm distance of the FOXA2-assayed sections using a kit (ab150686, Abcam; Waltham, MA) according to manufacturer instructions.
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bioRxiv - Neuroscience 2024Quote: ... The activity of cytosolic- or secretory-phospholipase-A2 was measured according to the procedures described in the kit manuals (Abcam, Cambridge, UK).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the sections were incubated with histofine simple stain kit Rat MAX-PO(G) (414331F, Nichirei, Tokyo, Japan) or goat anti-chicken IgY conjugated biotin (ab6876, abcam plc, Cambridge, UK) for 30 minutrs at RT ...
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bioRxiv - Immunology 2021Quote: ... Free fatty acid concentration in plasma (diluted 1:25) of clamped mice was determined using a quantitative Free Fatty Acid Assay Kit (Abcam, Cambridge, Great Britain). Absorbance was measured with the Filter Max F5 device (Molecular Devices ...
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bioRxiv - Cell Biology 2019Quote: ... we used the Maxpar Antibody Labeling Kit (Fluidigm) according to manufacturer’s instructions for addition of the respective metal tags: Axin2 (145-Nd, Abcam, ab32197, 2 µg/ml), p-MEK1/2 [S217/221] (151-Eu ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were rinsed with phosphate-buffered saline (PBS) and then stained in the culture well using the Adipogenesis Assay Kit (cell-based, Abcam Catalog No. ab133102). The dye was eluted using the kit’s elution buffer and quantified using a microplate reader at 490 nm wavelength ...
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bioRxiv - Immunology 2022Quote: ... media was collected and LDH activity measurement was carried out in 96-well plate (Costar, cat no. 3370) according to LDH assay kit (Abcam, cat. no ab102526). Absorbance measurement at 450 and 570 nm was carried out using microplate reader (BioTek ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were collected after 24 h and the caspase activity was determined using the Caspase-3 Assay Kit (Colorimetric) (Abcam ab39401, Cambridge, UK).
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bioRxiv - Molecular Biology 2021Quote: ... The serum is at the top layer and was collected for subsequent colorimetric analysis of the total cholesterol using the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, Cambridge, MA, USA) following manufactures protocol.
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bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
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bioRxiv - Physiology 2020Quote: ... and IL-10 concentrations were measured using ELISA kits (TNF-α: R&D Systems, Minneapolis, MN, USA; IL-1β, IL-6, and IL-10: Abcam), in accordance with the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553). Each experiment was repeated with similar results using anti-SARS-CoV-2 N rabbit polyclonal Ab (GeneTex # GTX135357 ...
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bioRxiv - Neuroscience 2023Quote: ... Triton X-100 in 1X PBS for 20 min and then endogenous biotin was blocked using Avidin/Biotin blocking kit (Abcam; cat. no. ab64212) to reduce non-specific background ...
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bioRxiv - Physiology 2023Quote: ... Unless specified below, all reagents were from MilliporeSigma (St. Louis, MO, USA). LDH assay kit (catalog no. ab65393) was from Abcam (Cambridge, MA, USA). P ...
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bioRxiv - Molecular Biology 2023Quote: The main pro-inflammatory and anti-inflammatory cytokines in blood and heart were measured by corresponding ELIZA kit: tumor necrosis factor-ɑ (TNF-ɑ, Abcam, ab100747), interleukin-1β (IL-1β ...
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bioRxiv - Cell Biology 2023Quote: The chymotrypsin-like activity of the 20S proteasome was assessed using a commercially available fluorometric proteasome activity assay kit (Abcam, catalog number: ab107921) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: Chromatin accessibility in isolated cardiac fibroblasts or whole heart from C57BL/6J mice was measured using the Chromatin Accessibility Assay Kit (Abcam, cat. no. ab185901) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... using V-PLEX Pro-inflammatory Panel 1 (Meso Scale Discovery, catalogue no. K15048D1) and mouse apolipoprotein E ELISA kit (abcam, catalogue no. ab215086) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2024Quote: The amount of collagen in liver tissues was determined by measuring the content of hydroxyproline using a Hydroxyproline assay kit (Abcam, Cat#MAK008-1KT) as described by the manufacturer ...