Labshake search
Citations for abcam :
2451 - 2500 of 2723 citations for Human Complement C3 Convertase C3c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and IgM-PE mAb (clone K52 1C3, BioRad Antibodies; conjugated using Lightning-Link® PE Antibody Labeling Kit, Expedeon, Abcam), for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: Glyceraldehyde 3 Phosphate Dehydrogenase Activity was measured by using Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay kit (Colorimetric) (Abcam®, #ab204732) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant fluid was added to 25 µL of pre-incubated protein G magnetic beads (ChIP-Adembeads ChIP-Adem-Kit, Ademtech SA) with 5 µg of anti-V5 rabbit polyclonal antibody (ab9116, Abcam) in IP buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... the induction of proliferation of naïve CD8+ T cells was assessed by BrdU Cell proliferation ELISA kit (Abcam, Cambridge, UK) according to the protocol of the manufacturer after 72 hours of co-culture ...
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bioRxiv - Genomics 2021Quote: Serum 17 beta-estradiol was measured using a randomized plate layout with 3 replicates per sample in female baboons on the LS and HS diets with the 17 beta Estradiol ELISA Kit (Abcam); human female serum (age 28 years ...
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bioRxiv - Bioengineering 2021Quote: ... 7′-Dichlorofluorescin diacetate (DCFDA) was used to measure intracellular ROS using the DCFDA Cellular ROS Detection Assay Kit (ab113851, Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: The brain ATP level was measured through an ATP Assay Kit according to the manufacturer’s instructions (Abcam, Catalog No. ab83355)10 ...
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bioRxiv - Neuroscience 2021Quote: Plasma samples were used to determine triglycerides levels with the TG assay kit for quantification (Abcam, ab65336; Cambridge, MA, USA) as previously described (Kalyan-Masih et al. ...
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bioRxiv - Cell Biology 2022Quote: Filipin III stocks were made in ethanol and stored in aliquots at −80 °C according to kit instructions (Abcam, ab133116). When ready for use ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µg of nuclear extract were used for the TP53 functional assay that was conducted according to the manufacturer’s instructions with the calorimetric p53 Transcription Factor Assay Kit (Abcam, ab207225). In short ...
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bioRxiv - Bioengineering 2022Quote: The XCL-1 measurement in mouse blood plasma samples was performed by using Mouse XCL- 1 SimpleStep ELISA kit (Abcam). The assay was performed as per manufacturer guidelines ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability of SHANK3-silenced PANC-1 cells treated with ERK and MEK inhibitors were measured using Cell Counting Kit-8 (WST-8 / CCK-8; Cat. no. ab228554, Abcam). Cells were silenced for 24 h as described above and then seeded on a 96-well plate with full medium containing DMSO (control) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... an aliquot of the reaction was used to determine the amount of synthesized ATP using ATP Assay Kit (Colorimetric/Fluorometric) (Abcam). The assay was performed at 37 °C ...
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bioRxiv - Microbiology 2022Quote: Membrane fluidity was measured as previously described (136) using a membrane fluidity kit that quantifies the fluorescence of a lipophilic dye (Pyrenedecanoic acid) (Abcam). Pyrene decanoic acid exists in monomeric and dimeric states within membranes ...
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bioRxiv - Systems Biology 2022Quote: ... Protein Assay Kit (Thermo-Fisher) and the activation of the c-Jun transcription factor was assayed by c-Jun Transcription Factor Assay Kit (Abcam) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 µm) were prepared and stained with 1% (w/v) toluidine blue and the Periodic Acid Schiff kit (ab150680, Abcam) as described in Hawkins et al ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The virus titer was quantified by an ELISA assay against the lentiviral envelope protein p24 (HIV1 p24 ELISA Kit, Abcam). The Cas9 expressing MEF cells were infected in presence of 8 µg/ml Polybrene with the lentiviral particles at a multiplicity of infection of 0,3 to avoid multiple gene knock outs in one cell ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was assessed after 72 hours using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, ab197010) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Subcellular fractionation of HeLa cells transfected with the ZC3H11A-Flag expressing plasmids (5μg plasmid/100mm tissue culture plate) was done using Cell Fractionation Kit (Abcam, ab109719) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Quantitative cAMP measurements were carried out on isolated cardiac mitochondria (0.5 mg per sample) using cAMP Direct Immunoassay Kit (Fluorometric) (Abcam, ab138880). Mitochondria samples were treated with 0 or 10 or 15 mM [HCO3−] ...
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bioRxiv - Molecular Biology 2022Quote: ... A TET activity assay was also performed on nuclear extracts using a fluorometric commercial assay (TET Hydroxylase Activity Quantification Kit, catalog# ab156913; Abcam) and microplate fluorometer (Synergy H1 ...
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bioRxiv - Molecular Biology 2022Quote: A DNMT activity assay was performed on nuclear extracts using a commercial assay (Colorimetric DNMT Activity Quantification Kit, catalog# ab113467; Abcam) and microplate spectrophotometer (Synergy H1 ...
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bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
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bioRxiv - Cancer Biology 2024Quote: ... non-esterified fatty acids (NEFA) and glycerol were determined using enzymatic kits (Triglyceride SL, Sekisui Diagnostics; NEFA-HR, Wako; glycerol, ab65337 Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Free glycerol was measured in the infranatant fraction of the isolated adipocyte culture using a colorimetric assay kit (ab65337, Abcam) following the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... Mitochondrial membrane potential in C2C12 myotubes was measured in a fluorescence plate reader (535 nm/580 nm (ex./em, respectively) with 50 nM TMRE and following the TMRE Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... Pan-flavivirus envelope protein antibody 4G2 was conjugated to APC using Lightning-Link® APC Conjugation Kit (abcam, Cat# ab201807). Cells were then blocked with Human TruStain FcX in FACS on ice for 10 minutes followed by staining with 4G2-APC in 1x Flow Cytometry Perm Buffer on ice for 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: EA.hy926 cells or EZH2 WT/mutant overexpressing HEK-293 treated with NO donor SNP (500 µM) for 2 hours were processed with the help of a Biotin Switch Assay kit (Abcam) using the standard manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... the resulting supernatant fractions were boiled for 10 min to inactivate enzymatic activity and their glycogen content measured using a Glycogen Assay Kit (ab65620, Abcam). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: AP-sites were tested in U2OS cells 48 h after transfection with control and UNG siRNA by a DNA damage assay kit (ab211154, Abcam) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: SA-bGAL activity was evaluated in the doxorubicin-induced senescent cells after 10 days using the Senescence Detection Kit (Catalogue number: ab6535, Abcam) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Metabolic activity within periosteal MSC was quantified using a Cell Counting Kit 8 assay (WST-8 / CCK8; Abcam, Cambridge, UK). Water soluble tetrazolium salts are used to quantify cell viability through the production of formazan dye similar to that of an MTT assay ...
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bioRxiv - Immunology 2024Quote: ... and were assessed in BMDM and RAW264.7 macrophage cells following exposure to 2-AA at the times indicated and by utilizing the ATP Assay kit (cholorimetric/fluorometric) (#ab83355, Abcam) and the PicoProbe AcCoA assay kit (ab87546 ...
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bioRxiv - Neuroscience 2023Quote: ... cellular lysates were incubated for 1h with LND or vehicle with was measured using a hexokinase assay kit (Abcam, ab211103) according to manufacturer instructions.
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bioRxiv - Cell Biology 2024Quote: ... adult mouse cardiomyocyte cell line HL-1 or primary neonatal mouse cardiac fibroblasts (NMCF) or cardiomyocytes (NMCM) using the Histone extraction kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The amount of cholesterol in cells and supernatants was assessed using the cholesterol/cholesteryl ester assay quantification kit (ab65359, abcam) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... spent media was collected for each condition and lactate secretion was assayed in duplicate or triplicate using either Lactate Colorimetric/Fluorometric kit (abcam) or Lactate-Glo (Promega ...
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bioRxiv - Immunology 2024Quote: ... Cellular ROS was also detected using the DCFDA/H2DCFDA cellular ROS assay kit according to the manufacturer’s instructions (Abcam; ab113851). To analyze cell populations ex vivo ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... that occurred per minute of incubation with each substrate at 37°C by Aspartate Aminotransferase Activity Assay Kit (Abcam ab105135) and the Alanine Transaminase Activity Assay Kit (Abcam ab105134) ...
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bioRxiv - Bioengineering 2024Quote: ... ELISA was performed on all serum samples after day 20 to measure the serum TTR level using Mouse Prealbumin ELISA kit (Abcam).
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bioRxiv - Cancer Biology 2024Quote: ChIP experiments were performed in duplicate for WT and MT cells (clones MT1 and MT2) using the High Sensitivity ChIP Kit (Abcam). 5x105 cells were used as input material for each immunoprecipitation ...
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bioRxiv - Cancer Biology 2024Quote: Surface-expressed EREG molecules/cell were quantified by labeling H231 with phycoerythrin (PE) with the Lightning-Link PE conjugation kit (Abcam) and BD QuantiBRITE PE ...
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bioRxiv - Biochemistry 2024Quote: ... 2 ml of cells were harvested by centrifugation and treated according to the standard protocol (TUNEL Assay Kit – FITC, ab66108, Abcam). Measurement of FITC fluorescence was performed with CytoFLEX cytometer (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... Caspase activation was assayed with the Caspase-3 and Caspase-1 colorimetric assay kits from abcam (ab39401 and ab273268, respectively). At harvest ...
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α-synuclein expression in response to bacterial ligands and metabolites in gut enteroendocrine cellsbioRxiv - Neuroscience 2023Quote: The amount of α-synuclein in the culture medium was measured using a mouse α-synuclein ELISA kit (Abcam ab282865) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and native histones that contain the modification and were purified from HEK293T cells using a Histone Extraction Kit (Abcam ab113476). Protein concentration was determined using the Pierce BCA assay ...
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bioRxiv - Molecular Biology 2023Quote: ... gel followed by either silver staining with Pierce Silver Stain Kit (Thermo) or Western blotting against anti-NOP58 (#ab236724, Abcam) and anti-GAPDH (#5174s ...
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bioRxiv - Neuroscience 2023Quote: ... were labeled with the fluorescent dye Alexa Fluor 647 according to the manufacturer’s protocol (Alexa Fluor 647 conjugation kit lightning link, Abcam, ab269823). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... 45 μL of reaction mixtures were added to 100 μL of glutamate detection reaction mix (Abcam Glutamate Assay Kit, ab83389) in a 96-well plate ...
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bioRxiv - Molecular Biology 2022Quote: ... 3’- diaminobenzidine (DAB) were done using the Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466, Abcam). Before the protein block ...