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Citations for abcam :
2401 - 2450 of 2459 citations for IL 4 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were blocked in 1% BSA in PBS for 30 minutes at room temperature before overnight incubation at 4°C in primary antibodies: α-mouse CENP-A (1:1000 Abcam ab13939 (discontinued) or 1:1500 Enzo ADI-KAM-CC006-E ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight: Anti-FMRP antibody (Abcam, ab17722, 1:1,000), anti-GAPDH antibody (Cell signaling technology ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked with Odyssey blocking buffer (Li-COR) and immunoblotted overnight (4°C) with rodent OXPHOS cocktail monoclonal antibody (Abcam; ab110413, 1:1000) to detect ETC proteins ...
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bioRxiv - Neuroscience 2023Quote: ... The slides with cryosectioned tissue were washed with PBS followed by incubation in blocking solution (2% of BSA in PBS) for 1 h and then incubated overnight at 4°C with an anti-DBI antibody (rabbit, 1:1000, Abcam, Cat. No: ab231910). Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000 ...
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bioRxiv - Pathology 2024Quote: ... The membrane was then probed overnight at 4°C with the primary antibodies (p105/p50, Abcam ab32360, 1:1000; Actin, Abcam ab3280, 1:1000) in Odyssey Blocking Buffer and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were blocked in 5% skim milk diluted in PBS-T for 1 hr at room temperature and incubated overnight at 4°C with a rabbit synaptophysin primary antibody (YE269, Abcam, Cambridge, UK; #ab32127) diluted 1:2,000 in blocking solution ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21: ab88572, Abcam; anti-GFP: ab290, Abcam). Paired-end sequencing was performed on a NovaSeq6000 platform (Illumina ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then incubated overnight at 4°C with the following primary antibodies in 2% w/v BSA: Rabbit anti-Gclc antibody (Abcam, United Kingdom; 1:1000); rabbit anti-NQO1 antibody (Cell Signalling ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP, 1:1000, Abcam; Ki67, 1:1000, ProteinTech). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... mice were fixed with 4% paraformaldehyde and the brain was sectioned at 200 μm thickness and immunostained against GFP (Abcam, no. ab13970, 1:300). Confocal microscopy (Zeiss LSM 700 confocal microscope ...
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bioRxiv - Physiology 2021Quote: ... Pre-synaptic motoneuron terminals were then labeled by incubating bundles overnight at 4°C in the same blocking solution to which had been added a mouse anti-synaptophysin antibody (AB8049 Abcam, USA; 1:25 dilution). The following morning bundles were washed (5 x 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in 5% milk TBS-T and incubated with blots at 4°C overnight (BMAL1, Abcam – ab93806; ACTIN, Abcam – ab3280, P84, Genetex – GTX70220). Blots were incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate ...
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bioRxiv - Physiology 2022Quote: ... 0,05% Tween 20 (TBS-t) for 60 min at RT and incubated overnight at 4°C with anti-IL6 antibody (Abcam, ab229381, 1:1000 dilution). Following 3 washes (10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then treated with 3% bovine serum albumin in PBS and incubated overnight at 4°C with the primary mouse antibody anti-Pax8 (Abcam ab53490, diluted 1/100). After washing unbound antibodies ...
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bioRxiv - Microbiology 2020Quote: ... before probing overnight at 4°C with antibodies raised to the following targets: goat polyclonal antibody to influenza A virus (ab20841, Abcam Inc., Toronto, ON, Canada), Mouse anti influenza A NA 1 (GeneTex ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4◻C in varying mixtures of the following primary antibodies: rabbit anti-DARPP-32 (1:1000; Abcam Cat# ab40801, RRID:AB_731843), mouse anti-Hu (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubations were performed for 24 hours at 4°C using an anti-glial fibrillary acidic protein (GFAP; Abcam, ab53554; 1:250 dilution) antibody ...
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bioRxiv - Microbiology 2020Quote: ... The rest of the chromatin was incubated overnight at 4°C with 1 μg of polyclonal anti-GFP antibody (Abcam ChIP grade, Abcam 290) or ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam, Cambridge, MA, USA), PD-L1 (ABclonal ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked in 5% skim milk in TBS + 0.1% Tween 20 (TBS-T) for 1 hour at room temperature before incubation in primary antibody overnight at 4°C (rabbit-α-caspase-1, Abcam #ab179515, 1:1000). Membranes were washed in TBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C: chicken anti-βIII tubulin (Tuj-1, 1:200; Abcam #ab41489; Boston, MA, USA), rabbit anti-PSD95 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... the fixed tissue sections were reacted individually overnight at 4°C with the following primary antibodies: rabbit anti-CD68 antibody (1:500, Abcam, Cat. #125212, Cambridge, MA), rabbit anti-CD146 antibody (1:500 ...
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bioRxiv - Genetics 2022Quote: ... for 1 hour at 4°C and then incubated overnight at 4°C with 1:1 mix of protein A/G Dynabead magnetic beads conjugated to H3K9me3 antibody (Abcam, cat# 8898 RRID: AB_306848).
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bioRxiv - Neuroscience 2024Quote: ... while the lower half was probed at 4 °C overnight with a 1:1000 dilution of a rabbit anti-β actin antibody (ab8227, Abcam, Waltham, MA, USA), in antibody dilution buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP; Abcam, ab3613; SantaCruz, sc-515550) or ...
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bioRxiv - Neuroscience 2023Quote: ... Following primary antibody incubation brains were washed 4 times for 15 minutes each in a 3% salt solution (in 1X PBST) and transferred to secondary antibodies (Abcam: ab175673; ThermoFisher: A-21236) in 1XPBST and incubated overnight at 4°C on a nutator ...
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bioRxiv - Molecular Biology 2023Quote: ... in methanol and non-specific binding blocked with 10% serum from the source species for the secondary antibody followed by a primary antibody incubation overnight at 4 °C (Sox9, abcam, ab185966,; p16, (abcam, ab241543), α-SMA (Sigma-Aldrich’ A5691) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated overnight in primary antibody in the blocking solution at 4°C using 1 or more of the following antibodies: α-SMA antibody (ab7817, 1:500, Abcam, Cambridge, MA, USA), FAP antibody (ab53066 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2% Tween 20) with 5% milk and incubated overnight at 4 °C with the primary antibodies: rabbit monoclonal anti-histone H2B (Abcam Cat#ab188291, 1:2000) or mouse monoclonal anti-beta actin (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained overnight at 4°C in blocking solution with the following antibodies and dilutions: Alexa Fluor® 488 anti-S100 beta (1:1000, Abcam, #ab196442, clone EP1576Y), PE anti-Nestin (1:150 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then incubated for 16 h O/N at 4°C with the following primary antibodies: CASR (Abcam Cat# ab79038, RRID:AB_2071489, 1:500), MAP2 (Abcam Cat# ab92434 ...
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bioRxiv - Biochemistry 2024Quote: ... After blocking 25 µL was removed and 25 µL of primary antibody solution in 1% BSA and 0.3% Triton-X100 in PEM was added to stain the cell body and flagella overnight at 4°C (anti-tubulin, Abcam #ab6161, 1:1000 dilution). Due to the amount of time required for imaging ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were added at 4 ºC overnight (Rabbit anti Lysozyme, Thermo Fisher Scientific PA129680, 1:50; Rabbit anti laminin, Abcam ab11575, 1:100). Samples were washed 5 x 1 hours with PBS at 4 ºC ...
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bioRxiv - Neuroscience 2024Quote: ... fol-lowed by 5% horse serum before incubation with the primary antibody MJF-14 (anti-conformation-spe-cific α-synuclein filament antibody MJFR-14-6-4-2, Abcam, #ab209538, 1:200). The immunohistology sig-nals were visualized with ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (VectorLabs ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS before incubation with the primary antibody MJF-14 (anti-confor-mation-specific α-synuclein filament antibody MJFR-14-6-4-2, Abcam, #ab209538, 1:25,000), overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... before blocking in 10% BSA and incubation with primary anti-bodies against aggregated α-synuclein (MJF-14; rabbit MJFR-14-6-4-2, Abcam, #ab209538, 1:25,000), total α-synuclein (mouse LB509 ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody incubation was performed overnight at 4°C using the following antibodies: anti-GFP rabbit (Abcam, diluted 1:2.000 in 5% milk in TBST) and anti-actin (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... The sample were incubated for 48 hrs at 4°C for secondary antibodies Alexa Fluor 647 (Donkey anti-Rabbit, Abcam ab 150075, 1:500 dilution) and Alexa Fluor 555 (Anti-mouse IgG (H+L) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated in primary antibody for 48 hours at 4°C (ChAT (1:200): goat anti-ChAT (Abcam Cat. #ab144P, Millipore, Temecula, CA), PV (1:2000) ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were applied in blocking buffer at 4°C over-night (alpha-tubulin, Abcam ab233661, 1:1000 dilution and Pcdh19, Abcam ab191198, in 1:200 dilution). After 3 wash steps in PBS-T (0.1% triton) ...
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bioRxiv - Physiology 2020Quote: ... Blots were probed using an antibody against one subunit of each of the mitochondrial oxidative phosphorylation complexes (Abcam #ab110412; 1:1000; overnight at 4°C) and followed by fluorescent secondary (1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were blocked in 5% BSA-TBST (TBS with 0.1% Tween20) and probed with anti-Padi4 (Abcam ab214810, 1:1000, O/N at 4°C) and anti-Actin (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ChIP-seq was carried out using 30 μg of chromatin isolated from cryopreserved PBMC samples obtained from two CLL patients and 4 μl of anti-EGR2 antibody (Abcam, cat# ab43020, Lot# GR101477-1). The immunoprecipitated DNA was processed into a standard Illumina ChIP-seq library and sequenced on an Illumina HiSeq instrument (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... primary antibodies used were for MHC (Developmental studies hybridoma bank #MF-20, Iowa City, IA, USA; 4 μg/mL) and Phalloidin-488 (Abcam #ab176753, Cambridge, UK; 1:1,000). Following primary antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblotting was next performed by incubating the membranes overnight at 4°C with the following primary antibodies: mouse monoclonal anti-mitofusin 2 antibody [6A8] (abcam, ab56889, dilution of 1/1000), rabbit polyclonal anti-mitofusin 2 (abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with a buffer at 4°C overnight prior to being incubated with primary antibodies anti-5-HT1A (Abcam #ab227165, rabbit pAb, 1:1000), anti-5-HT1B (Abcam #ab13896 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% non-fat milk powder in PBS-T followed by overnight incubation at 4°C with primary antibodies (anti-GFP; Abcam ab290 1:1000, anti-GAPDH; Abcam ab9485 1:10000, Cambridge, UK) 3x 5 minute washes with PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked with 5% w/v skimmed milk in TBST for one hour and then incubated at 4□ with primary antibodies (p-PI3K, Abcam, Catalog#ab278545; p-AKT(S473), CST ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated in 1%BSA and then overnight at 4 degrees in primary antibody (anti-GAD65/67, Abcam ab11070, 1:500 (Cheng et al., 2017)) ...