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Citations for abcam :
2251 - 2293 of 2293 citations for IL 4 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in 5% milk TBS-T and incubated with blots at 4°C overnight (BMAL1, Abcam – ab93806; ACTIN, Abcam – ab3280, P84, Genetex – GTX70220). Blots were incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate ...
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bioRxiv - Physiology 2022Quote: ... 0,05% Tween 20 (TBS-t) for 60 min at RT and incubated overnight at 4°C with anti-IL6 antibody (Abcam, ab229381, 1:1000 dilution). Following 3 washes (10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then treated with 3% bovine serum albumin in PBS and incubated overnight at 4°C with the primary mouse antibody anti-Pax8 (Abcam ab53490, diluted 1/100). After washing unbound antibodies ...
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bioRxiv - Microbiology 2020Quote: ... before probing overnight at 4°C with antibodies raised to the following targets: goat polyclonal antibody to influenza A virus (ab20841, Abcam Inc., Toronto, ON, Canada), Mouse anti influenza A NA 1 (GeneTex ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4◻C in varying mixtures of the following primary antibodies: rabbit anti-DARPP-32 (1:1000; Abcam Cat# ab40801, RRID:AB_731843), mouse anti-Hu (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubations were performed for 24 hours at 4°C using an anti-glial fibrillary acidic protein (GFAP; Abcam, ab53554; 1:250 dilution) antibody ...
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bioRxiv - Microbiology 2020Quote: ... The rest of the chromatin was incubated overnight at 4°C with 1 μg of polyclonal anti-GFP antibody (Abcam ChIP grade, Abcam 290) or ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam, Cambridge, MA, USA), PD-L1 (ABclonal ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked in 5% skim milk in TBS + 0.1% Tween 20 (TBS-T) for 1 hour at room temperature before incubation in primary antibody overnight at 4°C (rabbit-α-caspase-1, Abcam #ab179515, 1:1000). Membranes were washed in TBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C: chicken anti-βIII tubulin (Tuj-1, 1:200; Abcam #ab41489; Boston, MA, USA), rabbit anti-PSD95 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... the fixed tissue sections were reacted individually overnight at 4°C with the following primary antibodies: rabbit anti-CD68 antibody (1:500, Abcam, Cat. #125212, Cambridge, MA), rabbit anti-CD146 antibody (1:500 ...
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bioRxiv - Genetics 2022Quote: ... for 1 hour at 4°C and then incubated overnight at 4°C with 1:1 mix of protein A/G Dynabead magnetic beads conjugated to H3K9me3 antibody (Abcam, cat# 8898 RRID: AB_306848).
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP; Abcam, ab3613; SantaCruz, sc-515550) or ...
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bioRxiv - Neuroscience 2023Quote: ... Following primary antibody incubation brains were washed 4 times for 15 minutes each in a 3% salt solution (in 1X PBST) and transferred to secondary antibodies (Abcam: ab175673; ThermoFisher: A-21236) in 1XPBST and incubated overnight at 4°C on a nutator ...
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bioRxiv - Molecular Biology 2023Quote: ... in methanol and non-specific binding blocked with 10% serum from the source species for the secondary antibody followed by a primary antibody incubation overnight at 4 °C (Sox9, abcam, ab185966,; p16, (abcam, ab241543), α-SMA (Sigma-Aldrich’ A5691) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated overnight in primary antibody in the blocking solution at 4°C using 1 or more of the following antibodies: α-SMA antibody (ab7817, 1:500, Abcam, Cambridge, MA, USA), FAP antibody (ab53066 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2% Tween 20) with 5% milk and incubated overnight at 4 °C with the primary antibodies: rabbit monoclonal anti-histone H2B (Abcam Cat#ab188291, 1:2000) or mouse monoclonal anti-beta actin (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained overnight at 4°C in blocking solution with the following antibodies and dilutions: Alexa Fluor® 488 anti-S100 beta (1:1000, Abcam, #ab196442, clone EP1576Y), PE anti-Nestin (1:150 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then incubated for 16 h O/N at 4°C with the following primary antibodies: CASR (Abcam Cat# ab79038, RRID:AB_2071489, 1:500), MAP2 (Abcam Cat# ab92434 ...
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bioRxiv - Neuroscience 2024Quote: ... while the lower half was probed at 4 °C overnight with a 1:1000 dilution of a rabbit anti-β actin antibody (ab8227, Abcam, Waltham, MA, USA), in antibody dilution buffer ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody incubation was performed overnight at 4°C using the following antibodies: anti-GFP rabbit (Abcam, diluted 1:2.000 in 5% milk in TBST) and anti-actin (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... The sample were incubated for 48 hrs at 4°C for secondary antibodies Alexa Fluor 647 (Donkey anti-Rabbit, Abcam ab 150075, 1:500 dilution) and Alexa Fluor 555 (Anti-mouse IgG (H+L) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated in primary antibody for 48 hours at 4°C (ChAT (1:200): goat anti-ChAT (Abcam Cat. #ab144P, Millipore, Temecula, CA), PV (1:2000) ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were applied in blocking buffer at 4°C over-night (alpha-tubulin, Abcam ab233661, 1:1000 dilution and Pcdh19, Abcam ab191198, in 1:200 dilution). After 3 wash steps in PBS-T (0.1% triton) ...
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bioRxiv - Physiology 2020Quote: ... Blots were probed using an antibody against one subunit of each of the mitochondrial oxidative phosphorylation complexes (Abcam #ab110412; 1:1000; overnight at 4°C) and followed by fluorescent secondary (1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were blocked in 5% BSA-TBST (TBS with 0.1% Tween20) and probed with anti-Padi4 (Abcam ab214810, 1:1000, O/N at 4°C) and anti-Actin (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ChIP-seq was carried out using 30 μg of chromatin isolated from cryopreserved PBMC samples obtained from two CLL patients and 4 μl of anti-EGR2 antibody (Abcam, cat# ab43020, Lot# GR101477-1). The immunoprecipitated DNA was processed into a standard Illumina ChIP-seq library and sequenced on an Illumina HiSeq instrument (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... primary antibodies used were for MHC (Developmental studies hybridoma bank #MF-20, Iowa City, IA, USA; 4 μg/mL) and Phalloidin-488 (Abcam #ab176753, Cambridge, UK; 1:1,000). Following primary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with a buffer at 4°C overnight prior to being incubated with primary antibodies anti-5-HT1A (Abcam #ab227165, rabbit pAb, 1:1000), anti-5-HT1B (Abcam #ab13896 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% non-fat milk powder in PBS-T followed by overnight incubation at 4°C with primary antibodies (anti-GFP; Abcam ab290 1:1000, anti-GAPDH; Abcam ab9485 1:10000, Cambridge, UK) 3x 5 minute washes with PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked with 5% w/v skimmed milk in TBST for one hour and then incubated at 4□ with primary antibodies (p-PI3K, Abcam, Catalog#ab278545; p-AKT(S473), CST ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblotting was next performed by incubating the membranes overnight at 4°C with the following primary antibodies: mouse monoclonal anti-mitofusin 2 antibody [6A8] (abcam, ab56889, dilution of 1/1000), rabbit polyclonal anti-mitofusin 2 (abcam ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated in 1%BSA and then overnight at 4 degrees in primary antibody (anti-GAD65/67, Abcam ab11070, 1:500 (Cheng et al., 2017)) ...
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bioRxiv - Bioengineering 2021Quote: ... R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL; desmin, ab227651, Abcam, 1.32 μg/mL) were included in the AZP solution ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... 1% bovine serum albumin in PBS with 0.1% Tween20 [PBST]) for one hour at RT and left overnight in the A2AR primary antibody at 4°C (Abcam #ab3461, Lot # GR3431019-2, 1:100) in blocking solution ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and then incubated for approximately 20 hours at 4°C in either rabbit anti-α-synuclein (α-syn) phosphorylated serine 129 (P-S129; Abcam #EP1536Y, Cambridge, United Kingdom) diluted to 1:1000 in blocking solution or rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: chicken polyclonal anti-mouse GFAP (Abcam ref. ab4674, RRID AB_304558, 1:3000), rabbit polyclonal anti-mouse IBA1 (Wako ref ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin extract was sonicated with Bioruptor (20 min; pulse: 30 s on/30 s off; high amplitude) and immunoprecipitated with H3K27ac antibody (Abcam, ab4729, rabbit polyclonal, 4 µg/ChIP) premixed with Dynabeads A and G ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 1h in blocking buffer (PBS, 10 % FBS, 1 % Saponin) followed by primary antibody incubation overnight at 4°C (Calnexin, Abcam ab22595 at a dilution of 1:200). After washing twice with wash buffer (PBS ...
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bioRxiv - Microbiology 2023Quote: ... After washing twice with 200µl BD 1X Perm/Wash cells stained with conjugated CIITA antibody were fiyed in 2% PFA for 30 min at 4°C.Cells stained with GRN antibody were stained 1 hour at 4°C with secondary antibody goat anti rabbit (PE, Abcam #ab97070, 1:100 in 1X Perm/Wash). Cells were washed twice with 200µL BD 1XPerm/Wash solution and fixed in 2% PFA for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in primary antibody overnight at 4° C (chick α-GFP, Abcam, Cat# AB13970, Lot# GF305729-1; mouse α- γ-tubulin, Sigma-Aldrich, Cat# T6557, Lot# 066M4858V; rabbit α- α- tubulin, Abcam, Cat# AB233661) and in secondary antibody for two hours at room temperature (Alexa goat α-chick 488 ...