Labshake search
Citations for abcam :
2351 - 2400 of 2527 citations for Dengue Virus Serotype 4 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Between 300 and 500 µg of proteins were incubated with 1 µg of antibody (when using Santa Cruz or Abcam antibodies) or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... Resin was washed 4X in 800 uL EMB 300 Buffer and then protein association was analyzed by Western blot using antibody for hnRNP A2/B1 (Abcam #ab6102). Additionally ...
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bioRxiv - Neuroscience 2021Quote: ... Our antibody against DARPP-32 binds to a 16 AA segment on a region of the protein that does not contain any phosphorylation sites (Abcam ab40801) and therefore it labels DARPP-32 irrespective of phosphorylation state and cellular location ...
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bioRxiv - Immunology 2020Quote: ... For selected experiments T cells were cultured in serum-depleted medium with or without native human C1q protein (100ug/ml, Abcam, ab96363).
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were blocked with 5% fish gelatine before incubation with the primary antibodies for myelin basic protein (MBP, Abcam, 1:500) and protein gene product 9.5 (PGP ...
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bioRxiv - Systems Biology 2020Quote: ... The blot was probed with the antibody against the viral nucleocapsid protein (Cambridgebio, USA) and the horseradish peroxidase-conjugated Goat Anti-Rabbit IgG (Abcam, USA) as the primary and the secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the PNBM treated cell lysates were analyzed with a western blot assay to measure protein thio-phosphorylation with the anti-thiophosphate ester rabbit monoclonal antibodies (Abcam, USA).
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bioRxiv - Genetics 2020Quote: ... Reactions were stopped by addition of 6x SDS Laemmli buffer and proteins were resolved by 15% SDS-PAGE and analysed by western blotting with anti-ubiquitin antibodies (1:2000 Abcam P4D1) and anti-Rad51 (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... Staining was completed per manufacturer’s instructions for RNAScope® multiplex V2 detection kit (ACD Bio) coupled with immunofluorescent protein staining for either Cytokeratin19 (ab52625 Abcam) at 1:250 dilution ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The monoclonal anti-VGLUT2 antibody was raised in Mice immunized to a full length recombinant protein corresponding to rat VGLUT2 (Abcam, ab79157) and has been previously shown to stain VGLUT2 in zebra finches (86) ...
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bioRxiv - Neuroscience 2020Quote: ... The signal intensity from the examined proteins were evaluated on free-floating sections using the standard method of simultaneously combined staining (Abcam Protocol). Brain slices (50 μm ...
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bioRxiv - Neuroscience 2020Quote: ... at 100 μm thickness (coronal) and processed for free floating immunofluorescence using chicken anti Green-Fluorescent-Protein (GFP) primary antibody (1:1000 AbCam, ab13970), and Goat anti Chicken-alexa488 secondary antibody (1:500 thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... were resolved by SDS-PAGE and the target proteins were detected with Western blot (WB) using the antibodies as follows: GAPDH Rabbit monoclonal antibody (ab181602, Abcam, UK), SLC40A1 Rabbit polyclonal antibody (ab58695 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: Growth factor proteins were analysed in conditioned medium and dissociative tissue extracts by Western blotting using anti-HDGF from AbCam (ab131064), anti-CTGF from Santa Cruz (sc-14939 ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin levels were determined in duplicate using 150 μg of protein lysates per well using a sandwich ELISA assay (abcam, ab252364). Western blot and qPCR analyses were carried out as described above ...
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bioRxiv - Microbiology 2022Quote: ... Spain) binding to the 229E nucleocapsid protein and a goat anti-mouse secondary antibody conjugated with fluorescein isothiocyanate (Abcam, United Kingdom). TCID50 titers were calculated according to the method of Reed and Muench (44).
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bioRxiv - Genomics 2022Quote: ... Immunostaining was performed using a rabbit polyclonal to human SC lateral element protein SCP3 (1:500 dilution; Cat# ab15093, Abcam; RRID:AB_301639) and a Cy3-labeled donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... Chromatin immunoprecipitation was carried out using Protein A Dynabeads conjugated to approximately 1 μg of S139 rabbit γ-H2AX (Abcam 29893) or Rabbit H2AX (Merck Millipore 07627 ...
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bioRxiv - Microbiology 2020Quote: ... 50 μg of recombinant Rv3351c protein and anti-6xHIS antibody conjugated to horseradish peroxidase (HRP, 1:500; Abcam Inc., Cambridge, MA) were mixed together in PBS containing 0.5% BSA ...
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bioRxiv - Cancer Biology 2021Quote: Protein extraction and immunoblotting was performed according to standard procedures with primary antibodies against FLI1 (1:500, rabbit, ab15289, abcam, UK) and GAPDH (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... deparaffinized sections were rehydrated and stained using specific antibodies against Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5, ab219107, Abcam, USA), Lysozyme (ab76784 ...
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bioRxiv - Developmental Biology 2019Quote: Phospho-protein staining was performed as previously described (Okuda et al. 2018) using the following antibodies: GFP (abcam cat. no. ab13970), pErk1/2 (cell signaling technologies cat ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin immunoprecipitation was performed by incubation of the chromatin fraction overnight with 20 μl of protein-A coated beads (Thermo-Scientific, 53139) and 2 μg of H3K4me1 specific (Abcam, ab8895) or 2 μg of H3K4me3 specific (Abcam ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interests were detected using the following primary antibodies: anti-rabbit Catalase (1:375; catalog #Ab52477; Abcam, Cambridge, MA); anti-rabbit Glutathione Peroxidase (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... constant shaking) with the following primary antibodies: sheep anti-phospo-S6 ribosomal protein (pS6; 1:250, ab65748, Abcam, Cambridge, Massachusetts, USA); mouse anti-D3R (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 hour then incubated overnight with Anti-Amyloid beta precursor protein (Y188) rabbit monoclonal antibody at 1:1,000 (Abcam Cat# ab32136) and Anti-VCP mouse monoclonal antibody at 1:2,000 (Abcam Cat# ab11433) ...
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bioRxiv - Microbiology 2020Quote: ... or IgG were used to immunoprecipitate the chromatin fragments in the supernatant by overnight incubation using protein A sepharose beads (Abcam, UK) at 4 °C.The beads were washed twice with wash buffer ...
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bioRxiv - Microbiology 2021Quote: ... Proteins co-purified with His-NS5A or His tag protein were detected by western blot with mouse anti-Myc tag monoclonal antibody (Abcam, USA) for probing NEMO and mouse anti-His tag monoclonal antibody (Abcam ...
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Cancer Biology 2020Quote: S-nitrosylation of proteins was detected by western blot using an S-nitrosylation specific antibody (HY8E12 clone, Abcam # 94930, 1:20) or the Pierce S-Nitrosylation Western Blot detection kit (Thermo Fisher Scientific # 90105) ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was extracted from the 8 cultures using the lysis buffer from the GFP Fluorescent ELISA Kit (ab229403) (Abcam, Cambridge, UK) that was supplemented with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The quantitative detection of serum lipopolysaccharide binding protein (LBP) was performed by Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (Abcam, ab213876) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed at least three times with PBST and incubated with native Streptavidin protein DyLight 488 (1:750 dilution; Abcam, ab134349) in 0.5% fish skin gelatin with PBST for 2 hours ...
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bioRxiv - Physiology 2022Quote: ... 50-100 μg of protein were used for the pull down of FLAG-tagged Kcnq4 proteins with monoclonal anti-FLAG M2 antibody produced in mouse (mouse monoclonal Anti-FLAG M2 antibody, F3165) and Protein G Sepharose beads (Abcam, UK) at 4 °C overnight ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the included Pierce™ Protein A/G Plus agarose beads were incubated with rabbit anti-myc polyclonal antibody (ab9106, Abcam, USA) and cross-linked using 2.5 mM DSS crosslinker ...
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bioRxiv - Neuroscience 2022Quote: ... NF200 (1:500, Chemicon International, Temecula, CA, USA) rabbit monoclonal amyloid precursor protein (APP) antibody [Y188] (1:500, Abcam, Cambridge, MA) and NeuN (1:500 ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was transferred to a fresh Eppendorf tube and the protein concentration was assessed using Bradford reagent (Abcam, cat#ab119216) and stored at -80°C when not used immediately ...
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bioRxiv - Neuroscience 2023Quote: We processed hippocampal sections to assess the numbers of transit-amplifying hippocampal progenitors immunopositive for the transcription factor T-box brain protein 2 (rabbit anti-Tbr2, 1:500, Abcam, USA), immature neurons immunopositive for doublecortin (DCX ...
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bioRxiv - Molecular Biology 2023Quote: ... were coupled with 10μg of anti-protein of interest (POI; anti-Foxg1 ChIP-grade, rabbit polyclonal, Abcam #ab18259; anti-GFP, rabbit polyclonal, Abcam #ab290), or 10μg of rabbit IgG (Millipore #12370 ...
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bioRxiv - Neuroscience 2023Quote: ... 300μg of protein was used for immunoprecipitation assay using immunoprecipitation kit containing Dynabeads-coupled protein G (magnetic beads) and human anti-MMP9 (1:700) (Abcam, ab38898). Briefly the protein was mixed with anti-MMP9 antibody and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 1:4000 fold diluted rabbit anti-SARS-CoV-2 N protein-specific IgG (Abcam, Cat.#ab271180) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... nucleoplasm and chromatin fractions were used for protein analysis with western blotting with the following antibodies were used: anti-DHX9 (Abcam 26271), anti-TBP (Abcam 818) ...
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bioRxiv - Genetics 2022Quote: ATPase activity of cohesin was measured using EnzChek Phosphate Assay Kit with purified recombinant proteins depleted of free phosphate using Inorganic Phosphate Binding Resin (Abcam: ab270547). Reactions were assembled with10nM cohesin ...
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bioRxiv - Cancer Biology 2022Quote: Protein array analysis was performed using the Human MAPK Phosphorylation Antibody Array (ab211061) according to the manufacturer’s instructions (Abcam, Boston, MA). Visualization was performed with the ChemiDoc Touch Imaging System (Bio-Rad ...