Labshake search
Citations for abcam :
2001 - 2050 of 2355 citations for Dengue Virus Serotype 4 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... embryos were washed 3 times 10 minutes in 1x PBS with 0.1% Triton-X100 and 0.1% BSA and incubated overnight at 4°C with the secondary antibody goat-anti-mouse A568 1:500 (Abcam, antibody ID# ab175473). On the 4th day ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated on an orbital shaker for 24h at room temperature + 24h at 4 °C in polyclonal rabbit anti-mCherry (1:30 000, Abcam, cat. ab67453) or polyclonal chicken anti-EYFP (1:200 000 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sections were incubated overnight (16 h) in a humid chamber at 4°C with either rabbit SHH antibody (2 μg/ml; ab19897; Abcam, Cambridge, UK), mouse Col2 antibody (1/50 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Cell supernatant was collected 24h after changing cell medium then immediately filtered by centrifugation for 15 minutes at 4°C on a 10KDa column (Abcam or Millipore). This step eliminates proteins ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: Pull-down experiments were performed by following the method previously described.36 15-20 μg of acid extracted histones or nucleosomes isolated from HeLa cells were mixed with 50 μM of recombinant His6-tagged-BRPF2 bromodomain incubated at 4°C on a nutator for 60 min in the presence or absence of 10 μM BAY- 299 (abcam cat# ab230368) inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with the following primary antibodies: KLF4 (R&D, AF3640) and cyclophilin B (abcam, catalog ab16045). Chemiluminescent detection was performed with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was washed in TBST and incubated for 45 min in TBST containing 4% dried milk and HRP mouse anti-beta Actin AC-15 (1:25000, abcam Ref. ab49900). The membrane was washed in TBST and incubated 1 min in ECL Western Blotting Substrate (Thermo Fisher Scientific Ref ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12 ...
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bioRxiv - Microbiology 2022Quote: ... Mitochondrial membrane potential was measured using a tetramethylrhodamine ethyl ester (TMRE) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) as a control (Abcam, Cambridge, UK). Prior to incubation and acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated overnight at 4°C in the same solution containing a rabbit-anti-choline acetyltransferase (ChAT) monoclonal antibody (1:1000, Abcam ab178850, RRID:AB_2721842) and a guinea pig-anti-NeuN polyclonal antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; GAPDH ...
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bioRxiv - Physiology 2022Quote: ... primary antibodies were added in 50 μL blocking buffer for 12 hours at 4° C: ATP synthase antibody (Abcam, ab128743 1:200), sAC antibody (R21 IHC ...
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bioRxiv - Physiology 2022Quote: ... washed with PBS-T 3 times for 10min and incubated O/N at 4°C with primary antibody (Rabbit anti-Calnexin, Abcam, Cat# ab133615) at a dilution of 1:1000 in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then incubated in the following primary antisera overnight at 4°C in PBT: guinea pig antiinsulin (Abcam; Waltham, MA, USA), rabbit anti-glucagon (Santa Cruz ...
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bioRxiv - Neuroscience 2023Quote: ... The embedding blocks were cut on a cryostat into 20 μm thick sections and blocked for 30 min with 4% BSA and then incubated for 1 hour with the following primary antibodies: polyclonal rabbit anti-RFP (1:200, Abcam, Cambridge, UK).
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bioRxiv - Pathology 2023Quote: ... membranes were incubated overnight at 4 °C with agitation with primary antibodies against acetylcholinesterase (68 kDa, 1:500, ab97299; Abcam, Cambridge, UK), butyrylcholinesterase (75 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with primary antibodies diluted in blocking buffer overnight at 4°C - Mouse Anti-Human TRA1-81 (1:75, Abcam, AB16289#20), Rabbit Anti-Human OCT4 (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... LiCor) for 1 hour at room temperature and probed overnight at 4℃ with a monoclonal rabbit anti-HTT antibody (Abcam: EPR5526; Ab_109115) diluted 1:1,000 in blocking buffer plus 0.2% tween ...
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bioRxiv - Immunology 2023Quote: ... H3Cit staining of fixed surface labelled cells was performed by incubating the cells overnight at 4°C in PBS 2% HI-FBS + 2mM EDTA + 0.1% saponin with monoclonal anti-H3Cit antibody (Abcam, ab219407, 1:600). Cells were washed and then incubated with donkey anti-rabbit AF555 (Invitrogen ...
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bioRxiv - Genetics 2023Quote: Slides were incubated overnight at 4°C with the following antibodies diluted in PBS: rabbit anti-SYCP3 (ab15093, Abcam, 1:200 dilution), rabbit anti-SYCP1 (ab15087 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 48-72 hours at 4°C in primary antibodies: anti-Choline acetyltransferase 1:300 (Abcam catalog #: ab178850; RRID:AB_2721842), anti-Tyrosine hydroxylase 1:300 (Abcam catalog # ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated at 4°C overnight with a monoclonal antibody to human cytokeratin antigen (Abcam, Cat #ab53280, at 1:250), or monoclonal antibody to human POSTN (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in a humid chamber overnight at 4°C in primary antibody: anti-GFAP (Abcam [ab4674], 1:200 dilution), anti-Iba1 (Abcam [ab178846] ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with the appropriate primary antibody overnight at 4°C (anti-Histone H3(tri methyl K4) antibody (Abcam, ab8580; 1:50); anti-Histone H3(acetyl K27 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at 4°C overnight with primary antibodies including: NeuN (1:500, mouse, Millipore, Cat#:MAB377; 1:500, rabbit, Abcam, Cat#: ab104225), MBP (1:300 ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... The samples were incubated at 4℃ for 48 hours with mouse anti-CaMKIIα antibodies (ab22609, abcam, 1:250 dilution in 2% NGS). After treating the primary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibodies were diluted in 3% BSA/PBS and incubated overnight at 4°C: rabbit anti-RPA34 (Abcam ab97594, 1:1000), mouse anti-PML (Santa Cruz Biotechnology sc-966 ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed with 4% PFA (for 10 min) and stained with an anti-sarcomeric alpha actinin antibody [EA-53] (1:500; Abcam, cat#ab9465) followed by a phalloidin Texas Red- conjugated antibody (1:800 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were fixed in 4% paraformaldehyde for 15 min and stained with anti-γ-H2AX (CST,9718) and chicken anti-GFP (Abcam, #ab13970) primary antibodies in 1:400 and 1:600 dilution ...
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bioRxiv - Microbiology 2023Quote: ... bound complexes were isolated by overnight precipitation of the pre-cleared chromatin with 100 µL of blocked beads in combination with 3-4 µg of antibodies (anti-Histone H3 (Abcam ab17913, µg), Histone H3Ac (Millipore 17-615 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were then incubated overnight at 4°C with the following primary antibodies: anti-COX-2 (Abcam, UK, 1:1000 dilution), anti-IL-1β (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated on an orbital shaker for 24 hours at room temperature + 24 hours at 4 °C in polyclonal rabbit anti-mCherry (1:30 000, Abcam, cat. ab167453) or polyclonal chicken anti-EYFP (1:100 000) ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS) for 1h at room temperature and then incubated overnight at 4°C with primary antibodies: anti-melanopsin (Abcam, 1:200), anti-GFP (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were incubated ON at 4°C with the following primary antibodies in blocking solution: rabbit-anti-DYRK1A (Abcam, 1:100), mouse anti-DYRK1A (Abnova ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were incubated overnight at 4°C with rabbit monoclonal anti-syntaxin 2 antibody (7.8 µg/ml; Abcam, Cambridge, England, UK: ab170852) or goat polyclonal anti- SNAP23 antibody (1.25 µg/ml ...
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Suppression of the Integrated Stress Response in Islet β Cells Decreases Risk of Autoimmune DiabetesbioRxiv - Cell Biology 2023Quote: ... The blots were probed with the following primary antibodies and 0.2% Tween20 with overnight incubation at 4°C: anti-p-eIF2α (Abcam; ab32157; 1:1000)(Cell Signaling ...
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bioRxiv - Pathology 2024Quote: ... Membranes were then blocked for 1 hour with 5% dry milk in PBST (PBS supplemented with 0.1% Tween-20) buffer and incubated overnight at 4°C with primary antibody (anti-ACE2, Abcam; anti-Actin, Abcam) diluted in PBST containing 1% BSA (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with primary antibodies at the following dilutions: rabbit anti-MAP2 (ab254264 Abcam, 1:2000), mouse anti-βTUBULIN III (TUJ1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated overnight at 4°C with anti-TOMM20 (1:200 dilution) and anti-cytochrome C (1:200 dilution) primary antibodies (Abcam, Waltham, MA). Thereafter ...
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bioRxiv - Immunology 2024Quote: ... and incubated overnight at 4°C with the anti-F4/80 (#ab6640, 1:50) and/or anti-iNOS (#ab3523, 1:20) antibodies from Abcam (Cambridge, UK), and/or anti-AMPK alpha (Phospho Thr172 ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was incubated for 22-24 h at 4 °C in a solution of primary antibody (1:2000 rabbit polyclonal to c-Fos, Abcam, Cambridge, MA) with 5% normal goat serum (Abcam ...
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bioRxiv - Biophysics 2020Quote: ... All sections were permeabilized with 0.2% Triton X-100 in PBS for 15 minutes and non-specific protein binding was then blocked by incubation in a commercial blocking agent (ab156024, Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies against phospho-Heat Shock Protein 27 (S82) and phospho-c-Jun (S73) were obtained from Abcam (Cambridge, United Kingdom), while phospho-JNK (T183/Y185 ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted proteins were analyzed on a 6% SDS-PAGE gel followed by a western blot using monoclonal anti-GST (Abcam) or anti-MBP (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... one piece corresponding to the higher protein ladder size for the incubation of RAD21 and CTCF primary antibodies (RAD21: anti-RAD21 antibody, rabbit polyclonal, Abcam, ab154769 ...
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bioRxiv - Microbiology 2022Quote: ... α-surfactant protein D (primary: polyclonal, Abcam catalog no. ab203309; secondary: Donkey anti-Rabbit IgG, AlexaFluor 405, Abcam catalog no. ab175651), α-mannose binding lectin A (primary ...
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bioRxiv - Molecular Biology 2021Quote: Expression of GLIS1-βGAL fusion protein was examined by staining tissue sections with chicken anti-βGAL (1:500, ab9361, Abcam) and Alexa Fluor@ 488 donkey anti-chicken IgG (1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...