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Citations for abcam :
151 - 200 of 219 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Sections of 30 μm in the thickness were conducted for the CGRP sensory nerve immunofluorescent staining (1:100, ab81887, Abcam). All of the experiments were conducted following with the previous studies.30
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bioRxiv - Physiology 2023Quote: ... cells were equilibrated to room temperature and stained with PI for 30 minutes at 37°C using Propidium Iodide Flow Cytometry Kit (ab139418; Abcam) according to the manufacturer’s protocol and incubated at 37°C in the dark for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... or with recombinant TGF-β at 10 ng/mL (stock concentration 5 ng/µL) or Batimastat at 30 µM (stock concentration 1 mM, BB-94/Abcam). After 48 hours (or 1 hour for SMAD activity tests) ...
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bioRxiv - Microbiology 2024Quote: ... and 0.0001% Tween 20 in PBS at 37°C for 30 min and incubated with rabbit anti-Myc polyclonal antibody (1:100; ab9106, Abcam) or control normal rabbit IgG at 4°C overnight ...
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bioRxiv - Genetics 2024Quote: ... Reactions were incubated at 30°C for 30 minutes and then thiophosphates were alkylated by the addition of 2.5 mM p-nitrobenzyl mesylate (PNBM; Abcam ab138910) followed by incubating at room temperature for 2 hours (Lo and Hollingsworth 2011) ...
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bioRxiv - Systems Biology 2024Quote: ... 5% donkey serum and 0.1% Triton-X (antibody buffer) for 30 minutes at room temperature and treated with primary antibodies (Ki67 anti-Rabbit, 1:300, Abcam AB16667 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed in PBS then incubated for 30 min at 37°C in Neurobasal phenol-free working solution supplemented with TMRM 20 nM (Abcam), Calcein 4 µM (Insight Biotechnology) ...
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bioRxiv - Immunology 2024Quote: ... ∼50 µg of lysate material was treated with the reducing agent TCEP at a final concentration of 5 mM and heated to 60 °C for 30 minutes before being loaded onto a FASP protein digestion column (Abcam)75 ...
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bioRxiv - Bioengineering 2024Quote: ... at a concentration of 10 µM (Low dose, L) or 30 µM (High dose, H) or GSKJ4 (a histone demethyltransferase inhibitor, ab144395-Abcam) at concentration of 2 µM (L ...
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bioRxiv - Biophysics 2024Quote: ... The cells were then washed twice in ice-cold PBS and blocked for 30 minutes in PBS containing 2% donkey serum (ab7475, Abcam), 300 mM glycine (50046 ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were blocked in 1% BSA for 30 min and incubated with primary antibody against H3K79me2 (Abcam, ab3594, 1:1,000) for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Afterwards membranes were blocked for 30 min with 5% Nutrilon (Nutricia) in PBS and incubated overnight at 4°C with primary antibodies H3 (Abcam 1791), H4 (Merck Millipore 04-858) ...
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bioRxiv - Microbiology 2021Quote: ... Platelets were then permeabilized with 0.25% saponin for 30 min and stained using recombinant anti-Bcl-2 antibodies (AF647, Abcam, Berlin, Germany) for 30 min before being measured by flow cytometry ...
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bioRxiv - Neuroscience 2022Quote: ... in a heating chamber (37°C) in which the choroid plexus was loaded with Fluo-8 AM (5 μM, 30 min, Abcam, ab142773) with subsequent continuous wash with HEPES-aCSF (~1 ml/min ...
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bioRxiv - Cancer Biology 2022Quote: ... we incubated the slides in 1% BSA for 30 min at room temperature and staining was performed with Cytokeratin 20 1:100 (abcam, #ab76126) following the abovementioned Immunofluorescence staining protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were blocked with 1% normal goat serum (30 min at room temperature) followed by a goat anti-mouse IgG2a antibody (Abcam, ab98694). After additional blocking with normal goat serum ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were incubated for 30 min in serum/LIF medium supplemented with O-propargyl-puromycin (OP-Puro, 50 μM, Abcam, ab146664). Cells were then harvested ...
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bioRxiv - Developmental Biology 2020Quote: ... for 30 mins at room temperature and incubated overnight with 1: 400 diluted rabbit anti-SYCP3 antibody (1:200, Abcam, ab15093) at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then blocked with 5% Bovine Serum Albumin for 30 min and incubated overnight at 4°C with anti-RNAPII phospho S5 (1:1500, Abcam, ab5408), anti-RNAPII phospho S2 (1:3000 ...
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bioRxiv - Genetics 2020Quote: ... Embryos were washed (3x 30 min PBS-T) and then secondary antibody (goat anti-mouse Alexa Fluor® 555 - abcam ab150114) at 1:2000 and a nuclear counterstain (TO-PRO™-3 Iodide ThermoFisher Scientific T3605 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were blocked with 5% BSA in PBS for 30 min at room temperature and incubated with anti-Rad51(abcam ab133534) primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 2% BSA in 0.02% Tween-20 in PBS for 30 min at room temperature followed by overnight incubation with primary antibodies (rabbit polyclonal anti-GFP (1:4000, Abcam, ab290); mouse anti-GCP3 (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Erythrocytes were incubated for 30 min at room temperature in blocking buffer and stained directly on slides with anti-RNAP II Phosho-Ser2 (Abcam #5095) in the blocking buffer (1X PBS ...
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bioRxiv - Immunology 2022Quote: ... slides were performed on the automaton Leica Bond RX and unmasked at pH 6 before incubated 30 minutes with an anti-CD8 antibody (Abcam, ab98941) or CD4 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Intracellular ROS production in relation to mitochondrial mass was quantified using double staining of 30 μM dichlorofluorescin diacetate (DCFDA) (Abcam, # ab11385) and 150 nM MitoTracker Deep Red (MTDR ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were blocked in 3% -BSA/1x PBS for 30 min prior to incubation with fluorescence-conjugated Phalloidin (abcam, Cat# ab176753) at 1:000 for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... cells were left to incubate in primary antibody diluted in blocking solution for 30-45 min (chicken anti-GFP (Abcam ab13970) 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... This viral supernatant was then concentrated by centrifugation at 3200 x g for 30 minutes at 4°C before being resuspended in 100 µl virus suspension solution (Abcam, ab102538), aliquoted and stored at -70°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked for 30 min with 1% BSA previous 1h incubation at RT with the primary antibody rabbit anti-pericentrin (Abcam, ab4448), rabbit anti-pericentrin (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 minutes at room temperature and then incubated with the primary antibody (Amyloid Beta Rabbit Monoclonal – 1:500, Abcam, ab201060) overnight at 4 degrees C ...
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bioRxiv - Developmental Biology 2024Quote: ... for 30 min at room temperature and then incubated with primary antibodies as outlined below: mouse anti-CHD4 (1:500, Abcam, ab70469), rabbit anti-DDX4 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were stained in blocking solution (5% bovine serum albumin (BSA)) for 30 min at room temperature and then incubated with rabbit anti-LC3 (1:200, #ab48394, Abcam, Cambridge, UK) or rabbit anti-P62 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: Acute slices containing Neurobiotin-filled cells were fixed 30 min in 4% paraformaldehyde at 4°C and immunolabelled with antityrosine hydroxylase (chicken polyclonal, Abcam, 1:1000) and anti-AnkyrinG (mouse monoclonal IgG2b ...
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bioRxiv - Microbiology 2022Quote: ... Sera diluted at 1:2 were incubated for 60 minutes followed by a wash step and underwent 30 minutes incubation within an anti-ferret IgG conjugate (Abcam, Cambridge, UK) diluted to 1/200 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% penicillin/streptomycin in PBS (Ca2+,Mg2+)) containing 30 µg/mL Goat anti-human IgG H&L (Dylight® 488, Abcam ab96911) at a 1:1 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were blocked with PBS containing 0.1% saponin and 1% BSA for 30 min and incubated with primary mouse anti-LAMP1 antibody (Abcam, ab25630, 1:3000) or rabbit anti-CTSD antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed with Bond Wash solution 3x 30s then incubated with a rabbit anti-SOD2 primary antibody (ab13534, abcam, Cambridge, MA) 1:500 in Bond Primary Antibody Diluent for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... The primary antibodies were applied at room temperature for 30 minutes: CD3 (1:100, HIER 1, rabbit monoclonal, clone SP7, Abcam, UK; ab16669), CD4 (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated on an orbital shaker for 24h at room temperature + 24h at 4 °C in polyclonal rabbit anti-mCherry (1:30 000, Abcam, cat. ab67453) or polyclonal chicken anti-EYFP (1:200 000 ...
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bioRxiv - Bioengineering 2022Quote: ... for 2 hours followed by fixing for 30 minutes and a secondary stain for 2 hours (Abcam, Cat. No. Ab150077, 1:400). Type I and II collagen deposition was observed qualitatively through imaging.
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bioRxiv - Cell Biology 2024Quote: ... for 30 min at room temperature and incubated with the primary antibody against mouse UCP1 (ab234430, Abcam, UK; diluted at 1:500) at 4°C for 12 h ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained for 30 min with polyclonal fluorescein-isothiocyanate (FITC)-conjugated goat anti-mouse IgG H&L specific antibody (Abcam, United Kingdom) diluted 1:50 in RS-BSA 0.1% ...
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bioRxiv - Genomics 2023Quote: ... cells were fixed as in smFISH and blocked with co-detection antibody diluent for 30 min at room temperature prior to incubating with anti-human SC35 (Abcam, Cat # ab11826) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The slides were then blocked with 10% normal goat serum phosphate-buffered saline (PBS) for 30 min at room temperature (RT) and then were incubated with either Hamster anti-PDPN (Abcam Cat#: ab11936) or Rabbit anti-proSP-C (Millipore Sigma Cat# ...
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bioRxiv - Cancer Biology 2023Quote: ... and blocked with 1% BSA 0.05% saponin PBS for 30 min before incubation with anti-HA (bioLegend, Covance catalog #MMS-101P, 1:1,000) and anti-lamin-B1 (abcam ab16048, 1:1,000) antibodies in 1% BSA 0.05% saponin PBS 2 h at room temperature (RT ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated on an orbital shaker for 24 hours at room temperature + 24 hours at 4 °C in polyclonal rabbit anti-mCherry (1:30 000, Abcam, cat. ab167453) or polyclonal chicken anti-EYFP (1:100 000) ...
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bioRxiv - Neuroscience 2024Quote: Acute slices containing Neurobiotin-filled cells were fixed 30 min in 4% paraformaldehyde at 4°C and immunolabelled with anti-tyrosine hydroxylase (chicken polyclonal, Abcam, 1:1000) and anti-ankyrinG (mouse monoclonal IgG2b ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5% NP-40, in TBST) for 30 min at room temperature and then incubated with anti-GFP (1:2000, #ab290) (Abcam, Cambridge, United Kingdom) anti-Zld (1:750 ...
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bioRxiv - Cell Biology 2021Quote: ... CD63 staining was performed by incubating with mouse anti-CD63 (TS63 Diaclone 857.770.000 1/200) in PBS-BSA 1% for 30 min and CD9 staining was performed incubating with rabbit anti-CD9 (Abcam ab236630 1/80) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... PAs were then permeabilized with 0.2% Triton X for 30 minutes at room temperature followed by blocking with 5% normal donkey serum (Abcam plc, Cambridge, MA, USA) and 300 mmol/L glycine for one hour at room temperature ...