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Citations for abcam :
101 - 150 of 219 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The cells were then blocked using bovine serum albumin (PBS-BSA 1%) for 30 min and incubated with rabbit anti LAMP1 (Ab208943, Abcam) targeting the luminal domain ...
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bioRxiv - Cancer Biology 2020Quote: ... Antigen retrieval used citrate buffer pH9.0 with 30’ incubation, followed by staining for ER (SP1, 1:100) or MDC1 (Abcam ab11171 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated for 30 min with anti-LAMP-1 rabbit polyclonal antibody (1/100 final dilution, ABCAM) After washing ...
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bioRxiv - Immunology 2022Quote: ... The chelatable pool was determined by incubating cells with 30 μM pyridoxal isonicotinoyl hydrazone (PIH) (Abcam, cat no. ab145871) for 10 mins ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the membranes were washed three times with 5% nonfat dry milk in 0.1% TBST for 30 min each time and incubated with horseradish peroxidase-conjugated secondary antibody (Abcam) at room temperature for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... A1 reactive astrocytes were targeted for 48 h using neutralizing antibodies to IL-1α (30 ng/ml, Abcam, ab9614), TNF-α (30 ng/ml ...
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bioRxiv - Bioengineering 2021Quote: ... A2 reactive astrocytes were targeted for 48 h using neutralizing antibodies to IL-1β (30 ng/ml, Abcam, ab9722), TNF-α (30 ng/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were blocked at RT for 30 min in blocking solution (1x PBS3T 5% normal goat serum (Abcam ab138478)) and incubated in primary antibody dilution buffer (antibody diluted in 1x PBS3T and 1% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1% Triton X-100 in PBS for 30 minutes and incubated with anti-murine CD31 (1:1000, Abcam, ab124432) and anti-human mitochondria (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 m at RT and incubated in primary antibody mouse anti-Nup153 (Abcam) at 1:250 for 30 m ...
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bioRxiv - Physiology 2024Quote: ... HC-030031 (to block TRPA1 channels; 10 µM for 30 min, from a 50 mM stock in DMSO, Abcam), N-acetyl-L-cysteine (NAC ...
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bioRxiv - Neuroscience 2023Quote: ... slices received the chemical induction protocol for late LTP that consisted of three applications (10 min duration each at 30 min intervals) of the mGluR1/5 agonist (S)-3,5-dihydroxyphenylglycine (DHPG, 5 μM; Abcam) in the presence of the mGluR5 antagonist 2-methyl-6 (phenylethynyl)-pyridine (MPEP ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked with 3% FBS in PBS for 20 min and then incubated 30 min with 0.3 ug of anti-GLUT1 (clone EPR3915) (Alexa Fluor 647 conjugated, Abcam_ab195020) on ice ...
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bioRxiv - Bioengineering 2024Quote: ... No. 30-2300) were purchased from ATCC. 4,6-diamidino-2-phenylindole (DAPI) (Cat. No. ab228549) was purchased from Abcam. EGFP mRNA-lipid nanoparticle (LNP ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated in 1% BSA (bovine serum albumin) in 1X PBST (1X PBS + 0.1% Tween 20) for 30 min blocking using Normal Goat Serum (ab7481, Abcam) at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated at 37°C for 30 min with complete DMEM containing 5 µM Zinpyr-1 (Abcam, ab145349), Hoechst 33342 (0.2 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... for 30 minutes followed by normal horse serum for 1 hour prior to staining with CD68 (1:150) (Abcam), CD86 (1:800 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded in 6-well plates for 24h prior to 30 minutes incubation with 10µM EdU (Abcam, #ab146186). Cells were then harvested ...
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bioRxiv - Molecular Biology 2020Quote: ... Complex I activity was determined by following change in 450 nm absorbance per minute (mOD/min) for 30 minutes following the addition of Assay Solution (Abcam).
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used as primary antibodies: monoclonal mouse anti-lamin A/C (1:30; ab40567; Abcam, Cambridge, UK), mouse anti-γ-tubulin (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were blocked in 20% NGS for 30 min (Ki67) or 5% NGS for 60 min (CC3) before incubation in primary antibody overnight (anti-Ki67, Abcam; anti-cleaved Caspase 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Captured cells were immunostained by 200μL of an antibody cocktail containing 30 μg/mL anti-pan cytokeratin AF488 (Abcam, ab277270), 6 μg/mL anti-cytokeratin 18 FITC (LifeSpan BioSciences ...
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bioRxiv - Pathology 2021Quote: ... All sections were incubated in PBS-FCS 10% for 30 min and anti-lamin A/C antibody (dilution 1/200, clone EPR4100, Abcam) diluted in PBS-FCS 5%-BSA 1.5% was then added for overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were blocked with 5 % normal goat serum in Tris B for 30 minutes and incubated overnight at 4 °C with a rabbit polyclonal primary antibody against mCherry (1:3000, #167453, Abcam) diluted in blocking solution ...
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bioRxiv - Bioengineering 2021Quote: ... 5 x 106 beads were then incubated with an H3K9me2 antibody for 30 minutes with rotation at 4°C in 100 μL of 1% PBSA (ab1220 1:200, Abcam). Samples were then washed with 1% PBSA and incubated with secondary antibody for 10 minutes with rotation at 4°C in the dark in 100μL 0.1% PBSA (ab150107 1:250 ...
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were then incubated for 30 min with 50 μl solution containing 1% BSA in PBT and streptavidin-Alexa 488 conjugate (Abcam) diluted 1:150 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were blocked in 5% foetal bovine serum (FBS) for 30 min and incubated overnight in rabbit anti-PSD-95 (1:100; Abcam, Cambridge ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation for 30 min at room temperature and/or incubation overnight at 4 °C with primary antibodies (Rabbit anti-NKCC1: 59791, Abcam, 1:400 in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... cells were blocked with 5% BSA in PBS for 30 min and incubated for one hour with the primary antibodies: alpha-1 antitrypsin (AAT; Abcam) or albumin (Alb ...
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bioRxiv - Systems Biology 2021Quote: ... cells were incubated with 3% BSA and 0.1% Triton X-100 for 30 min at RT and stained with anti-gamma H2AX (Abcam 2893), anti CENP B (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... 30-minute incubation at room temperature): goat anti-rabbit conjugated to Alexa Fluor 594 at a 1:500 dilution (Abcam), goat anti-mouse conjugated to Alexa Fluor 488 at a 1:500 dilution (Abcam) ...
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bioRxiv - Cancer Biology 2021Quote: ... heat-induced epitope retrieval was performed by boiling the samples in a steamer for 30 minutes in antigen unmasking solution (Abcam, antigen retrieval buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The sections were blocked in normal goat serum for 30 minutes and incubated overnight at 4°C with the primary antibodies against TCF1 (Ab96777, Abcam; 10 µg/ml for knee ...
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bioRxiv - Biophysics 2020Quote: ... then incubated to 37°C for 30 min in the dark with 4 µg/ml of Pyronin Y (abcam, ab146350), diluted in FACS buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in 30 ml of lysis buffer (100 mM Tris. HCl, 150 mM NaCl, EDTA-free protease cocktail inhibitor (1:200, Abcam), 2 mM DTT) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100uM glycine for 30 minutes at room temperature and labeled overnight at 4C with primary antibodies (rabbit anti-FOXP2 Abcam #16046 1:500 ...
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) containing 1% BSA for 30 mins and incubated overnight with anti-H4K8ac antibody (1:100; ab15823, Abcam) and with Alexa Fluor 594 secondary antibody (1:300 ...
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bioRxiv - Genomics 2021Quote: ... 30 µg of proteins were used for each western blot and proteins were detected using anti-SUMO2/3 (ab3742, Abcam) and anti-TBP (ab51841 ...
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bioRxiv - Immunology 2021Quote: ... heat-induced epitope retrieval was performed by boiling the samples in a steamer for 30 minutes in antigen unmasking solution (Abcam, antigen retrieval buffer ...
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bioRxiv - Neuroscience 2020Quote: ... followed by blocking in donkey serum for 30 min and incubating overnight at 4 □ with primary antibody (GPX4, rabbit, ab125066, 1:200, Abcam) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The bead/chromatin complexes were resuspended in 200 μL of TE buffer and incubated 30 min at 37 °C with 10 μg of RNase A (Abcam), as well as input DNA ...
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bioRxiv - Genetics 2022Quote: ... The cleared lysate was incubated at 37 °C for 30-mins and subjected to Western blot analysis using an anti-GFP antibody (Abcam) to detect POPDC1-CFP containing complexes ...
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bioRxiv - Neuroscience 2022Quote: ... for 30 min followed by overnight incubation at 4° in primary antibodies, Aβ (Novus, NBP11 -97929) and NRN1 (Abcam, ab64186). Membranes were washed with 1×Tris-buffered saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Immunology 2022Quote: ... we fluorescently labeled ∼300,000 CAR T cells at 37°C for 30 minutes in 1:1 (v/v) solution of live cell staining buffer (Abcam) and RPMI-1640 (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged at 1,000 g for 30 minutes before proceeding to ELISA (Human FAS Ligand ELISA Kit - CD95L –Abcam-ab100515).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slices were blocked with 5% goat serum (Beyotime, China) for 30 min and then incubated with anti-Aβ1-42 (1:300, Abcam, UK), anti-Tau(pSer396 ...
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bioRxiv - Genomics 2024Quote: ... These strains bearing single-copy integrations were grown overnight at 30°C in rich medium YEA and used for ChIP with 2 µl of anti-GFP (ab290, Abcam). PCR oligonucleotides detecting central core (cc1&3 ...
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bioRxiv - Immunology 2024Quote: ... heat-induced epitope retrieval was performed by boiling the samples in a steamer for 30 minutes in antigen unmasking solution (Abcam, antigen retrieval buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... the cells were incubated at 4□ for 30 min with an anti-Tra-1-85 antibody (Abcam, ab108308, 1:100) diluted in PBS including 1% BSA and then stained with a AlexaFluor 555-conjugated donkey anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Blocking was performed with 2% BSA in PBS for 30 min and cells were incubated with phalloidin-iFluor 594 reagent (1:1000; ab176757; Abcam) for 1 hour.