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Citations for abcam :
151 - 200 of 1168 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The pellet was centrifuged once again and re-suspended in nuclear extraction buffer from the kit to generate the nuclear fraction and samples used for the NF-κB p65 transcription ELISA based assay (Abcam, ab133112) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... the samples were rinsed with PBS three times before and after staining the nuclear DNA with NucBlue and Phalloidin-iFluor 647(Abcam, ab176759) for 30 minutes at room temperature.
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bioRxiv - Genetics 2024Quote: ... the samples were incubated in rabbit anti-DPY-27 antibody (purified antibody) [50] at 1:200 dilution and mouse anti-nuclear pore complex [Mab414] antibody (Abcam ab24609) at 1:1000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... and histones were extracted using a histone extraction kit (Abcam, Cat# ab113476), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Proteins were stained using InstantBlue Coomassie protein stain (Abcam). Molecular weights were manually estimated according to the Novex Sharp protein marker ...
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bioRxiv - Developmental Biology 2021Quote: ... Non-decalcified E16.5 and P0 samples had mineralized bone visualized by von Kossa staining (n=3 per age and genotype) with Nuclear Fast Red counterstaining as part of an established protocol (Abcam, Cambridge, UK). For osteoclast staining ...
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bioRxiv - Pathology 2020Quote: ... We used primary mouse monoclonal antibodies against anti-α-acetylated tubulin (clone 6-11B-1, Sigma Aldrich and proliferating cell nuclear antigen (PCNA, clone PC10, Abcam) for detecting mitotic spindles and proliferating cells ...
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bioRxiv - Pathology 2023Quote: ... regularly spaced (500 µm) along the rostrocaudal axis were stained with a Prussian blue and nuclear fast red stain kit (Abcam, Cambridge, UK) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2020Quote: Bulk histones were isolated from cells or tissue using Histone Extraction Kit (Abcam) and resolved on 4-12% Bis-Tris Plus Bolt™ gels at 4-5 ug of protein per lane in MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... solani mutants were isolated with a Lipopolysaccharide Extraction Kit (Abcam, Symbios, Gdansk, Poland) as described previously 57 ...
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bioRxiv - Cell Biology 2023Quote: ... 8 days adipocyte differentiated MEFs using the EpiSeeker Chromatin Extraction Kit (Abcam, ab117152). ChIP analyses were carried out as described previously (Reintjes et al. ...
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bioRxiv - Cell Biology 2023Quote: ... tissues were homogenized in chilled 1× Cell Extraction Buffer (5X PTR; ab193970, Abcam), incubated on ice for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 mg of nuclear extract was incubated overnight on a rotating wheel at 4°C with primary antibodies MTHFD2-rabbit (Abcam; #ab151447; 4 μg), MTHFD2-mouse (Abcam ...
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bioRxiv - Physiology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Reagent (Abcam, USA). Proteins were separated on SDS-PAGE and transferred to the polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2022Quote: ... lysed and the histones were extracted by using the Histone Extraction Kit (ab113476, Abcam) the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Protein quantification was performed with the BCA protein assay kit (ab207003, Abcam). Proteins were separated by SDS-PAGE and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Oxysterol-binding protein-related protein 8 (anti-OSBP8/ORP8; Abcam, ab99069), anti-Cell cycle control protein 50A (anti-CDC50A/TMEM30A ...
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bioRxiv - Microbiology 2024Quote: ... Proteins on the gels were visualized using InstantBlue Coomassie Protein Stain (Abcam), destaining with distilled water.
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NK cells orchestrate splenic cDC1 migration to potentiate antiviral protective CD8+ T cell responsesbioRxiv - Immunology 2020Quote: ... 1% Protein Block (Abcam), and 2% 2.4G2 supernatant ...
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bioRxiv - Physiology 2020Quote: ... and protein phosphatase (Abcam) inhibitor cocktails ...
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bioRxiv - Physiology 2022Quote: ... CFTR protein (ab2478, Abcam) and Na,K-ATPase α-subunit (ab76020 ...
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bioRxiv - Neuroscience 2023Quote: ... or Protein G (Abcam) beads were added and incubated overnight to preclear the supernatant ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: ... LSBio #LS-C401108 and α-Caveolin- 1, R&D #AF5736; α-Trpv4 or α-Caveolin-1 only or α-Caveolin-1 and α-nuclear HistoneH3 Abcam #ab8898 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein A sepharose beads (ab193256) and protein G sepharose beads (ab193259) were from abcam.
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bioRxiv - Immunology 2021Quote: ... S1 Spike protein was detected using a Covid-19 S-protein ELISA kit (Abcam) specific to S1RBD ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant human PD-L1 protein and recombinant human tau protein were purchased from Abcam. HRP-conjugated goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2019Quote: Protein carbonyl assays used the Oxidized Protein Western Blot Detection Kit (ab178020; Abcam, USA) and were performed as we previously described.36 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and NICD: Mdm2 (MDM2 protein Gln119-Leu438 Origene TP761916; MDM2 protein-full length Abcam, ab82080 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins on the gel were visualized by staining with InstantBlue Coomassie protein stain (Abcam).
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bioRxiv - Developmental Biology 2023Quote: ... Protein concentrations were determined using the BCA Protein Quantification Kit (Abcam, Cambridge, UK, ab102536) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... recombinant mouse WNT3a protein (Abcam) 50ng/ml was added and cells cultured for 8 hours before lysis in Trizol.
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bioRxiv - Bioengineering 2020Quote: ... Collagen IV protein (Abcam, ab7536) and Matrigel (growth-factor reduced ...
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bioRxiv - Physiology 2019Quote: ... total OXPHOS protein (Abcam, #ab110413), mt-Co2 (Proteintech ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated Protein-G (abcam), and Alexa Fluor 488/555-conjugated anti-goat/mouse IgG donkey polyclonal antibodies (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant MAP2c protein (Abcam, #ab114686), carrying a ∼26 kDa Glutathione-S-Transferase (GST ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein blocking solution (Abcam). They were then incubated overnight at 4°C with primary antibody (Mouse TIF-IA (Abcam) ...
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bioRxiv - Immunology 2023Quote: ... fibroblast activation protein (FAP, Abcam rabbit monoclonal antibody EPR20021 ...
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bioRxiv - Molecular Biology 2023Quote: ... green fluorescent protein (ab290, Abcam) Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... followed by protein block (Abcam) for 30 minutes and were then incubated with relevant primary antibodies diluted in antibody diluent (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... De novo protein synthesis was measured using the Global Protein Synthesis Assay Kit (Abcam, ab235634) following the instruction provided by the vendor ...
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bioRxiv - Biochemistry 2021Quote: ... Carbonyls in protein samples were quantified using the Protein Carbonyl Content Assay Kit (Abcam, ab126287) according to the manual ...
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bioRxiv - Neuroscience 2020Quote: ... The molecular weights of the proteins were verified using a prestained protein ladder (ab116028, Abcam). Data were analyzed by densitometry in ImageJ using the gel quantification plugin.
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Guanine Nucleotide Binding protein (G protein Beta 3 subunit, GNB3; Abcam; 1:1000), rabbit anti-β-catenin (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were measured using the BIO-RAD Protein Assay or Bradford assay (Abcam 119216) and adjusted with apoplast extraction buffer to a final concentration between 0.5-1.5 mg/mL ...
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bioRxiv - Immunology 2023Quote: Protein A was quantified in the CM using the Protein A ELISA Kit (abcam, UK) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Heat shock 70 kDa protein 8 Lipopolysaccharide-associated protein 1 (HSC 70, Abcam Cat. # AB78431)
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bioRxiv - Physiology 2021Quote: Carbonyl groups inserted into proteins by oxidative reactions were evaluated with protein carbonyl assay kit (Abcam). Briefly ...
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bioRxiv - Microbiology 2022Quote: The level of oxidized protein was measured with the Oxyblot kit (Protein Carbonyl Assay Kit, Abcam) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Target proteins were normalized to total cellular/housekeeping proteins: anti-α-actinin (Abcam, ab18061, 1:3000), anti-β-actin (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: Protein-protein interactions between Galectin-3 and FcRn were studied using Co-Immunoprecipitation Kit (ab206996, Abcam) (see Supplementary methods).