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Citations for abcam :
101 - 150 of 1168 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-Human Nuclear Antigen (clone 235-1) (Abcam Cat#ab191181, RRID: AB_2492189), rabbit polyclonal anti-Ki67 (Abcam Cat#ab66155 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were resolved using 10% SDS PAGE and probed with nuclear (U170K, Abcam ab83306) marker antibodies at 1:1000 dilution and cytoplasmic (GAPDH ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was applied at a 1:1,000 dilution with 3 μM Nuclear Green DCS1 (Abcam) for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 1:100 for the Anti-Lamin B1 antibody - Nuclear Envelope Marker (Abcam, cat#. 16048), and 1:100 for the Anti-Histone H3 (di methyl K9 ...
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bioRxiv - Neuroscience 2022Quote: ... and nuclear compartments were incubated with antibodies for phospho-S118 ERα (1:1000, Abcam) and total ERα (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... hepatic nuclear factor 4 alpha (HNF4a, hepatocyte marker; 1:100; Abcam Cat# ab41898, RRID:AB_732976), and phosphorylated c-Jun (pcJun ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclei were incubated with mouse anti-Human Nuclear Antigen [235-1] (Abcam, ab191181) antibody at 1:200 dilution and sorted using a BD FACSAria II sorter ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-neuronal nuclear NeuN (targeting nuclei of neurons, 1:200, Abcam #ab177487, USA), followed by the slices being washed three times for 30 min each with PBST ...
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bioRxiv - Microbiology 2024Quote: ... Cytoplasmic and nuclear fraction supernatants were incubated with 1ug of α-HA antibody (Abcam 9110) and 25uL Protein G Magnetic Dynabeads (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... Cytoplasmic and nuclear fraction supernatants were incubated with 1ug of α-HA antibody (Abcam 9110) and 25uL Protein G Magnetic Dynabeads (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclear envelope was stained using primary rabbit anti-Lamin A/C antibody (ab133256, Abcam; 1:500) and Alexa-Fluor-647-labelled secondary anti-rat (Molecular Probes 1:400 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1mg of nuclear extract was incubated with the indicated antibodies: 2.5ug of BRD4 (Abcam; ab128874), 5ug of MED1 (Bethyl Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Histone H3 as a nuclear marker was detected using 1:1,000 anti-histone H3 antibody (Abcam) and 1:2,000 anti-rabbit antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... coverslips were mounted on glass slides using fluoroshield mounting medium plus DAPI fluorescent nuclear stain (Abcam). Slides were dried ...
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bioRxiv - Neuroscience 2023Quote: ... the secondary antibody solution was replaced by solution consisting of the nuclear stain Hoechst (Abcam, ab228550) at a concentration of 1/2000 diluted in PBS and incubated at a shaking table at RT for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... we used Histone Extraction Kit (Abcam, cat # ab113476) and followed manufacturer protocol ...
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bioRxiv - Biochemistry 2023Quote: ... After a period of 24h nuclear extracts (∼80 μL) were generated using a kit from Abcam (ab113474). Resultant nuclear extracts were aliquoted into single-use tubes (∼10 μL each at ∼1 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... Histones were extracted with Histone Extraction Kit (ab113476, ABCAM). Proteins were separated by SDS-PAGE and transferred to PVDF membranes ...
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bioRxiv - Immunology 2022Quote: ... Histones were extracted with Histone Extraction Kit (ab113476, Abcam). Nuclear and cytoplasmic extracts from CD8+ T cells were obtained with the NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833 ...
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bioRxiv - Genetics 2024Quote: ... histone extraction was performed (Abcam, Cambridge, UK, Cat# Ab113476) with five whole 3rd instar larvae (n=8 replicates ...
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bioRxiv - Biochemistry 2021Quote: HDAC activity of HCT116 nuclear extract was assayed using the Fluorometric HDAC Activity Assay kit from Abcam (ab156064) according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... mitochondrial and nuclear fractions were isolated from cells in 10cm dishes using the Standard Cell Fractionation Kit (Abcam) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Aliquots of cytoplasmic and nuclear extracts were analyzed via immunoblot for biotinylation efficiency using streptavidin-HRP (Abcam, ab7403) and for miniTurbo–TAF protein expression using a rat anti-HA-HRP antibody (3F10 ...
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bioRxiv - Cancer Biology 2022Quote: ... Total histone was extracted using histone extraction kit (ab113476, Abcam) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... extracts were made using the Histone Extraction Kit (Abcam #ab113476). For total protein ...
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bioRxiv - Cancer Biology 2023Quote: ... histone was extracted using a Histone Extraction Kit (ab113476, Abcam) according to the manufacturer’s instructions and analyzed by immunoblot.
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bioRxiv - Genetics 2023Quote: ... Histones were isolated with a Histone Extraction kit (Abcam ab113476). Protein concentration was assessed with the Pierce BCA Protein Assay kit (ThermoFisher Scientific 23225) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then treated with 90µl of Extraction buffer (Abcam), snap frozen in liquid nitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Histone lysates were extracted using the Histone Extraction kit (Abcam). Whole protein lysates were generated through cell lysis in RIPA buffer and pelleting of non-soluble debris ...
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bioRxiv - Immunology 2024Quote: Histones were extracted by following the histone extraction protocol (Abcam) for western blots ...
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bioRxiv - Biochemistry 2024Quote: ... Histone extraction was performed following a specific protocol from Abcam. Protein concentration was determined using the Bradford assay (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Pluripotency was assessed by immunostaining with surface and nuclear pluripotency markers (SSEA-4, Oct4 and Nanog, Catalog #ab109884, Abcam) for subsequent imaging.
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bioRxiv - Cell Biology 2021Quote: ... anti-Acta2 (1:100) and anti-nuclear transcription factor Gli family zinc finger (Gli)2 (1:100; ab26056, Abcam) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Diluted nuclear extracts were incubated with anti-RNA polymerase II CTD repeat YSPTSPS antibody (8WG16)-ChIP grade (Abcam, ab817) overnight at 4°C ...
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Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionbioRxiv - Neuroscience 2021Quote: ... cells were collected and separated into nuclear and cytoplasmic fractions according to manufacturer recommended subcellular fractionation protocol (https://www.abcam.com/protocols/subcellular-fractionation-protocol) ...
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bioRxiv - Cancer Biology 2023Quote: We extracted nuclear and cytoplasmic fractionation of A375 and MeWo cells following treatment according to manufacturer’s instructions (Abcam, ab113474). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... Attached cells were then fixed by methanol/acetic acid solution (3:1) stained with Perl’s Prussian blue with nuclear fast red as counterstain (Iron stain kit, ab150674, abcam). Slides were dehydrated (incubation with 75 ...
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bioRxiv - Physiology 2020Quote: Liver lipids were extracted with Lipid Extraction Kit Chloroform Free (Abcam). Total and cholesterol ester (Millipore/Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Total histone was extracted using a histone extraction kit (ab113476, Abcam) as per manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Dissected cochlea were placed in NADH/NAD extraction buffer (Abcam, ab65348) and homogenized with a micro pestle ...
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bioRxiv - Cancer Biology 2023Quote: Cell fractionations were prepared using Chromatin Extraction Kit from Abcam (ab117152) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... based on hematoxylin and eosin (H&E) staining and nuclear immune-positivity for human-specific NCL (ab13541, Abcam, Cambridge, UK) as previously described [21] ...
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bioRxiv - Genomics 2021Quote: ... Validation of Myc-tagged RARaa protein expression was done by western-blotting on cytosolic and nuclear lysate fractions from injected embryos using a ChIP-grade anti-myc antibody (ab9132, Abcam).
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bioRxiv - Molecular Biology 2022Quote: ... A TET activity assay was also performed on nuclear extracts using a fluorometric commercial assay (TET Hydroxylase Activity Quantification Kit, catalog# ab156913; Abcam) and microplate fluorometer (Synergy H1 ...
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bioRxiv - Molecular Biology 2022Quote: A DNMT activity assay was performed on nuclear extracts using a commercial assay (Colorimetric DNMT Activity Quantification Kit, catalog# ab113467; Abcam) and microplate spectrophotometer (Synergy H1 ...
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bioRxiv - Microbiology 2023Quote: ... a 1:50 dilution of an antibody against nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 (ab7970 Abcam) and 1:200 phalloidin coupled to Alexa Fluor 568 ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was dried for 1 min (the time should be extended if the tissue section is not dry) before being stained with 1 mL Nuclear Fast Red (NFR, ab246831, Abcam) for 10 minutes at RT at a leveled surface and rinsed again as described for SB staining ...
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bioRxiv - Cell Biology 2022Quote: ... the post-nuclear fractions prepared from the LLOMe-treated or untreated cells were incubated with anti-LAMP-1 primary antibody (Abcam, #ab24170) or IgG control (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were removed and nuclear extracts were incubated overnight at 4°C with either anti-lamin A/C antibody (Abcam, #ab108595) or non-immune IgG ...
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bioRxiv - Genetics 2023Quote: ... slides were stained for goblet cells and mucins using an Alcian Blue dye and Nuclear Red Fast counterstain kit (Abcam; ab150662). Quantification of the percent area of Alcian blue staining ...