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Citations for abcam :
1801 - 1850 of 2957 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Mouse AST ELISA Kit (Abcam), Mouse TNF-alpha Quantikine ELISA Kit (R & D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... ACTA2 (aSMA) ELISA kit (Abcam); TGFb ...
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bioRxiv - Cancer Biology 2024Quote: ... the histone extraction kit (abcam) was used according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and ChIP kit (Abcam, ab117138) according to manufacturer’s protocols ...
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bioRxiv - Genetics 2024Quote: ... Fast BC kit (Abcam, England), EpiMark® BC kit (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: A mitochondria isolation kit (Abcam) was used to perform mitochondrial isolation ...
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bioRxiv - Cell Biology 2020Quote: ... gels were transferred to PVDF membranes at 25 V in 4 °C overnight and incubated with ATP5a antibody (Abcam, ab14748, 1:3000), followed by anti-mouse secondary antibody.
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bioRxiv - Neuroscience 2021Quote: ... membrane was probed with the following primary antibodies for overnight at 4°C: Rabbit anti-mouse LINE-1Orf1p (Abcam, ab216324, 1:1000), Mouse anti-GAPDH antibody (HRP conjugated ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound antibody was removed by five 5-min washes and the membrane was incubated with alkaline phosphatase-conjugated goat anti-mouse secondary antibody or goat anti-human IgG secondary antibody (Abcam, Cambridge, UK). Membranes were washed again and then developed using SuperSignal ELISA Pico Chemiluminescent Substrate Kit (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed with 1X TBS-T prior to incubating with HRP-conjugated goat anti-rabbit secondary IgG (ab6721, Abcam, Cambridge UK) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 5 repeats of 5 μL aliquots were transferred to a nitrocellulose membrane which was then blocked before incubation with the anti-α-syn primary antibody MJFR1 (diluted 1:1000, abcam, UK). The membrane was subsequently incubated with the goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was incubated with the following primary antibodies: anti-KLK10 (BiossUSA bs-2531R, 1:1000) anti-GAPDH (Abcam ab23565, 1:2000), anti-β-actin (Sigma-Aldrich A5316 ...
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with secondary antibody for 1 hr at room temperature: rat-HRP 1:10,000 dilution (abcam ab205720 Lot# GR32115155-1), rabbit-HRP 1:10,000 dilution (Santacruz sc-2004 Lot#B2216) ...
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bioRxiv - Physiology 2022Quote: ... Membranes were incubated in the following primary antibodies overnight at 4°C: polyclonal rabbit anti-beta actin IgG (Abcam Cat# ab8227, RRID:AB_2305186) at a dilution of 1:1,000 ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1h with their respective secondary fluorescent antibodies: anti-mouse (1:2,500, IRDye 800, Abcam, Cat# ab216772, RRID: AB_2857338) and anti-rabbit (1:2,500 ...
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bioRxiv - Cancer Biology 2021Quote: ... These membranes were then blocked with 5% milk or 5% BSA and probed with antibodies specific to RBM3 (Abcam Cambridge, United Kingdom), VEGFA (Santacruz Dallas ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at RT before immunoblotting with antibodies against ACC1 (#ab72046, Abcam, 1:2000), ACC2 (#HPA006554 ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from harvested cells were extracted with RIPA-lysis buffer and 15-30µg total protein was separated using 10% denaturing polyacrylamide gel followed by transfer methanol activated-PVDF membrane and was probed with anti-c-Myc antibody (ab32072, Abcam, Cambridge, UK). The blots were developed in X-ray film using an ECL kit (Invitrogen ...
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bioRxiv - Pathology 2023Quote: ... membranes were incubated overnight at 4 °C with agitation with primary antibodies against acetylcholinesterase (68 kDa, 1:500, ab97299; Abcam, Cambridge, UK), butyrylcholinesterase (75 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were then incubated overnight at 4°C with the following primary antibodies: anti-COX-2 (Abcam, UK, 1:1000 dilution), anti-IL-1β (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with goat-anti-mouse IgG secondary antibody conjugated with horseradish peroxidase (ab6789, Abcam, Cambridge, UK; 2 mg/ml) at a final concentration of 0.8 μg/ml / in 1:2500 dilution in milk blocking buffer for 1.5 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cell lysates were subjected to gel electrophoresis and transferred to membranes that were incubated with an antibody against AMPKα1 (Abcam cat. no. ab32047), DNMT1 (Cell Signaling cat ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was first developed with myosin VI antibody (Table S1) and in a second step incubated with GFP antibody (Abcam, Table S1) and developed a second time ...
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bioRxiv - Biochemistry 2024Quote: ... the membrane was incubated (overnight, 4 °C) with the anti-α-syn primary antibody MJFR1 (diluted 1:1000 in PBS-Tween, Abcam, UK). Following rigorous washing with PBS-T ...
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bioRxiv - Genetics 2024Quote: ... Proteins were transferred to PVDF membranes by wet transfer and incubated with specific primary antibodies: anti-γ-H2A (ab15083, Abcam, 1:1,000), anti-Rad53 (ab166859 ...
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bioRxiv - Microbiology 2022Quote: ... Slides were stained using ‘Elasic Stain Kit (Verhoeff Van Gieson/EVG Stain)’ kit (Abcam, ab150667) according to manufacturer instructions ...
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bioRxiv - Physiology 2022Quote: ... The Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) kit was purchased from Abcam (ab150667). Slides were placed into elastic stain solution for 15 minutes and rinsed with cool ...
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bioRxiv - Immunology 2021Quote: ... labeled with FITC and DyLight 405 (DyLight® 405 Conjugation Kit, FITC Conjugation Kit, Abcam), at 4 °C for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... lysates were diluted twofold in assay buffer and incubated with capture beads for FUT8 (protein G-agarose beads; Abcam) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used for RNA-binding protein immunoprecipitation (RIP) assays: anti-H1.3 (ab24174, Abcam, 5 µg/reaction), anti-H1.5 (ab18208 ...
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bioRxiv - Neuroscience 2020Quote: Cell viability was measured after 3 days and 5 days of culture using an MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (Abcam, ab211091) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: RSV titre in cell-free supernatant was quantified by immunoplaque assay using biotinylated goat anti-RSV polyclonal antibody (Abcam). HEp-2 cells were infected with dilutions of RSV-containing supernatant for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... media samples were collected after Seahorse analysis to determine lactate concentration using the L-lactate Assay according to manufacturer’s protocol (Abcam). Absorbance values at 450 nm were obtained using a plate reader ...
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bioRxiv - Bioengineering 2023Quote: ... The PC-12 neurite outgrowth assay was initially validated by probing for β-tubulin III (1:1000; Abcam, ab7751) using ICC to optimize the positive ...
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bioRxiv - Molecular Biology 2024Quote: ... Kinase assays were performed in vitro by incubating 100 ng/µl recombinant human RNAPII-CTD (Abcam, ab81834, >95% purity) for 15 min at 30°C in the presence of 50 ng/µl CDK11s/Cyclin L ...
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bioRxiv - Microbiology 2024Quote: Neutrophil respiratory burst was measured using a dihydrorhodamine 123 fluorescent assay in a 96-well plate (Abcam, Waltham, MA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to PVDF membranes and immunodetection of respiratory chain complexes was performed using the Total OXPHOS Blue Native WB Antibody Cocktail (Abcam, ab110412, 1:250). The membrane was imaged using a Chemidoc XRS+ System (BioRad).
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bioRxiv - Molecular Biology 2020Quote: ... Bottom halves of dermal sample membranes were incubated with rabbit anti-TIMP3 (Cell Signalling Technology; D74B10) and top halves with mouse anti-HSA (Abcam, ab10241; loading control). Bottom half of epidermal sample membranes were incubated with rabbit anti-RPL36 (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then blocked (5% milk in TBS-Tween buffer) and incubated for 18 h with primary antibodies: CD44 (Abcam, 1:1000 dilution); GFAP (ThermoFisher ...
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bioRxiv - Pathology 2021Quote: ... The membrane was then probed overnight at 4°C with primary antibodies (P105/p50, 1:1000, Abcam ab32360; Actin, 1:1000, Abcam ab3280) in Odyssey Blocking Buffer and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred to 0.2 um nitrocellulose or PVDF membranes and detected with primary antibodies targeting LRRK2 (ab133474, Abcam, RRID: AB_2713963, 1:500), pS935 LRRK2 (ab133450 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the organoids on the insert membrane were transferred to a glass slide with two drops of mounting medium containing DAPI (Abcam 104139, Cambridge, UK), and a coverslip was applied.
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bioRxiv - Neuroscience 2020Quote: ... so membranes were also incubated with a mouse monoclonal anti-beta actin primary antibody (dilution: 1:5000, product number: ab8226, Abcam, Cambridge, MA, USA). After TBST washing three times for 10 minutes each wash ...
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bioRxiv - Immunology 2022Quote: ... The membranes were incubated at 4°C overnight with the following primary antibodies: α- GADD153/CHOP (SCBT sc-166682) and α-Tubulin (Abcam ab176560). After washing ...
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bioRxiv - Neuroscience 2020Quote: ... Levels of α-synuclein recruited to synaptic membranes were detected for each condition by Western blot with a rabbit polyclonal pan-synuclein antibody (1:1000; Abcam ab53726; Cambridge, MA) and quantified using ImageJ.
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bioRxiv - Cancer Biology 2020Quote: ... the membranes were placed in 5% nonfat milk for blockage and probed with the primary antibodies against TEAD4 (ab151274, Abcam, Cambridge, MA, USA), PKM2 (ab137852 ...
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bioRxiv - Microbiology 2020Quote: ... the fixed cells were labeled with primary antibodies including goat anti-major outer-membrane protein (MOMP; Meridian, Memphis, TN) and mouse or rabbit anti-six histidine tag (Genscript, Nanjing, China and Abcam, Cambridge, UK, respectively). For experiments using CRISPR interference ...