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Citations for abcam :
1701 - 1750 of 2957 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked for 20 min with Rotiblock (Roth #A151.1) and incubated with an anti-WDR5 specific antibody (Abcam #ab56919) in Rotiblock for 20 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times with TBST and incubated with HRP-conjugated goat anti-human IgG (Fab’)2 (Abcam) or HRP-conjugated anti-rabbit IgG (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... then the membranes were incubated with the primary antibodies (Rabbit anti-P2Y12, DF10263, Affinity Biosciences, Beijing, China; Rabbit Anti-TOMM20, ab186735, Abcam, Cambridge ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the membrane was incubated with the rabbit anti-IMPA1 antibody (1:10000 diluted in 5% BSA in 0.1% TBST) (Abcam ab184165) or goat anti-CHRM1 antibody (1 µg/ ml ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 8–10 h with the following primary antibodies: anti-CLPP (ab124822, Abcam, Cambridge, UK), anti-PTEN-induced kinase 1 (PINK1 ...
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bioRxiv - Bioengineering 2023Quote: ... Transferred nitrocellulose or PVDF membranes were subjected to immunoblotting with a monoclonal antibody anti-Cas9 antibody (Abcam: ab189380 or ab191468), anti-FLAG antibody (Cell Signaling Technologies ...
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bioRxiv - Genetics 2023Quote: ... The membranes were then incubated 1 hour at room temperature with horseradish peroxidase conjugated goat anti-rabbit IgG (Abcam, ab6721). Signal detection was achieved using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal Egfr (Abcam, ab52894; 1:500), rabbit anti-pEgfr Y1068 (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against the following targets were used to probe the membranes: rabbit polyclonal anti-PP1c (1:1000, Abcam, Cambridge, MA), rabbit polyclonal anti-PPP1R12C (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), BACE1 (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was washed and incubated first in blocking buffer and then with an anti-m6A antibody (Abcam, ab151230, USA) overnight at 4 °C with gentle shaking ...
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bioRxiv - Systems Biology 2023Quote: ... PVDF membranes were blocked and stained with total OXPHOS human western blot antibody cocktail (Abcam Cat# ab110411, RRID:AB_2756818, 1:500). Blots were visualized using an Odyssey infrared imaging system (RRID:SCR_022510 ...
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride membranes were incubated overnight at 4°C with various antibodies: rabbit anti-DDIT4 (1:1000, ab191871, Abcam), rabbit anti-TRIM13 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The membranes were probed with appropriate antibodies overnight at 4°C (anti-rabbit histone H3 (abcam, ab1791; 1: 2,500 dilution), anti-mouse FLAG antibody (Sigma ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: A panel of cytokines was measured in the supernatant of the neuron/astrocyte/microglia tricultures using a mouse inflammation antibody array membrane (Abcam), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The membranes were then washed in TBS-T and incubated with horseradish peroxidase-labeled (HRP) goat anti-rabbit secondary antibody (Abcam ab97080 ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Primary antibodies were diluted in blocking buffer and incubated with the membrane overnight at 4° C (1:1,000 anti-CD4, Abcam #ab133616). After primary antibody incubation ...
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bioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were probed for total tau (DA9; 1:1,000) and GAPDH (as a loading control, Abcam; 1:5,000). HA-tagged intrabodies were probed with monoclonal Anti- HA (1:1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam, 1:3000 dilution) and EP1536Y (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... Subsequently membranes were incubated with primary antibodies (1:1000 rabbit anti-LEDGF-PWWP [Abcam; ab177159], 1:1000 rabbit anti-GAPDH [Abcam; Ab9485] ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were washed three times with PBS-T and incubated with a 1:5000 HRP-conjugated secondary antibody (Abcam, ab205718), diluted in 5 % BSA in PBS-T ...
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bioRxiv - Microbiology 2024Quote: ... which was transferred to a nitrocellulose membrane and blotted with an anti-Tir antibody19 and anti-GroEL (1:100,000) antibody (Abcam ab69617). GroEL was used as a loading and lysis control.
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bioRxiv - Physiology 2024Quote: ... The native gel was then stained with Coomasie Blue or transferred to a PVDF membrane and subjected to western blot for subunit c of ATP synthase (ab181243, Abcam).
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299; ERK1/2, Abcam, ab184699 ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked (5% milk (PanReac AppliChem, A0830)) and probed with the following antibodies: anti-β-Tubulin (Abcam, ab15568), anti-GAPDH (Proteintech ...
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bioRxiv - Biochemistry 2024Quote: ... Purification was assessed by SDS-PAGE in 12% resolving gels either stained with PageBlue or by transfer onto a PVDF membrane and western blotting with the monoclonal primary antibody anti-Adenosine Receptor A2a (ab79714, Abcam) at 1 µg/mL and the secondary antibody anti-mouse IgG HRP at a 1:3000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... The upper part of the membrane was incubated with Rabbit recombinant monoclonal anti-mouse HIF-1 alpha antibody [EPR16897] (ab179483, abcam) at a 1:500 dilution in 0.5% BSA in TBS-T ...
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bioRxiv - Physiology 2024Quote: ... Aliquots of lysates were filtered through 10K Amicon filters and glutathione and CoA were measured in the flow-through using the following kits: Glutathione detection kit (Cayman 703002) and CoA detection kit (Abcam ab102504). Metabolite concentration was normalized to the protein concentration in lysates ...
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bioRxiv - Pathology 2024Quote: Plasma und urine NGAL as well as urine albumin were determined using commercial ELISA kits (Mouse NGAL ELISA Kit, LifeSpan BioSciences, Seattle, USA; Albumin Simple Step ELISA Kit, Abcam). Results were normalized to plasma protein content as a corrective measure for fluids given ...
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bioRxiv - Neuroscience 2020Quote: ... Differentiation was characterized by immunofluorescence assay with neuronal cytoskeleton markers βIII-Tubulin (1:500, Abcam, Cambridge, UK) and neurofilament (SMI-312 ...
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bioRxiv - Neuroscience 2020Quote: The CSF and serum albumin were measured by corresponding enzyme-linked immunosorbent assay (ELISA) (ab108788, abcam, USA) and colorimetric method respectively ...
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bioRxiv - Bioengineering 2020Quote: ... Glycogen levels were determined using a standard assay according to manufacturer’s instructions for fluorometric detection (Abcam, #ab65620). Glucose quantification assay ...
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bioRxiv - Cancer Biology 2022Quote: Protein expression of CXCL2 (MIP-2) was quantified using an enzyme-linked immunosorbent assay (ELISA; Abcam ab184862). Cell culture supernatant was collected after 24 hours and ELISA was performed following the manufacture’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... ChIP assay was performed in both estradiol untreated and estradiol treated groups using the H3K9ac (Abcam, ab16635) and H3K4me3 (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Brain and serum Li+ content was assayed using a commercially available colorimetric assay (Abcam, item no. Ab235613). Whereas typical clinical laboratories can measure serum Li+ down to ∼ 0.3 mM ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibody used in this assay was goat anti-rabbit IgG H&L (HRP) (Abcam, 205718) at 1:5000 for p-SMAD2 and p-SMAD3 and 1:15000 for SMAD2 and SMAD3 ...
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bioRxiv - Microbiology 2022Quote: ... samples were analyzed by immunoblot assays using the following primary antibodies: monoclonal anti-p30 antibody Ab130757 (Abcam); monoclonal anti-C9 antibody Ab5417 (Abcam ...
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bioRxiv - Physiology 2024Quote: ... The ROS activities were quantitated with DCFDA/H2DCFDA – Cellular Reactive Oxygen Species Detection Assay (Abcam cat# ab113851). Both assays were used according to protocol recommended by manufacturer.
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bioRxiv - Molecular Biology 2022Quote: The ROs were pooled (n=10) and lysed in radioimmunoprecipitation assay (RIPA) protein lysis buffer (Abcam, UK) with protease inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... 50µl of reaction mix (47µl Glucose uptake assay buffer, 2µl of enzyme mix and 1µl of PicoProbe, all supplied by Abcam) was added to each sample and standard well ...
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bioRxiv - Molecular Biology 2023Quote: Cell cytotoxicity was evaluated by a commercially available CytoScan™ WST-1 Cell Cytotoxicity Assay (ab65473; Abcam).
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bioRxiv - Physiology 2023Quote: ... OCR and ECAR values were normalized to protein levels within each well quantified by BCA assay (Abcam) conducted after the Seahorse assay.
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bioRxiv - Genomics 2023Quote: ... The following commercial control qPCR assays were also used: GAPDH positive control ChIP primer pair (Abcam ab267832), Human Positive Control Primer Set MYT1 (Active Motif ...
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bioRxiv - Cell Biology 2023Quote: ... the dephosphorylated TFEB samples were subjected to a kinase assay with 300 nM IKKα (Cat# ab102103, abcam), IKKβ (Cat# 81066 ...
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bioRxiv - Microbiology 2024Quote: ... Immunoblotting assays were performed with anti-GFP rabbit polyclonal antibody (BBI) or anti-Myc polyclonal antibody (Abcam) as the primary antiboty and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (BBI ...
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bioRxiv - Neuroscience 2021Quote: The Mouse MCP1 ELISA Kit and Mouse IP-10 ELISA Kit from Abcam (cat no ...
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bioRxiv - Cancer Biology 2024Quote: ... Mitochondrial protein fraction was isolated according to kit protocol (Mitochondrial Isolation Kit, Abcam). The following antibodies were employed to determine protein abundance ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked in 5% milk for 1h followed by incubation with primary antibodies: c-Myc (Abcam ab185656; 1:1000), Fos (Cell Signaling 2250S ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed three times with PBS-T and then incubated with secondary HRP-conjugated anti-rabbit IgG (Abcam, Cambridge, UK) or anti-mouse IgG (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were then incubated with primary antibodies (Lox, rabbit, 36 KDa, ab31238, 1:600 dilution, Abcam; 4-hydroxynonenal (4-HNE), rabbit ...