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Citations for abcam :
1651 - 1700 of 1718 citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... in phosphate buffered saline (PBS) prior to overnight incubation at 4°C with blocking buffer containing primary antibodies raised against HIS48 (Abcam, Ab33760, 1:20), CD68 (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... milk and the membranes incubated at 4°C overnight with rabbit Anti-Annexin A1/ANXA1 antibody monoclonal antibody [EPR19342]-BSA and Azide free (1: 25000, Abcam, Cat. N. ab222398), ALIX (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Slides were then stained with the primary antibody diluted in 10% BSA in TBS-T overnight at 4 °C: FAP (Abcam, ab207178, 1:250) and POSTN (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated with primary antibodies (ON; 4°C) that were appropriately diluted in blocking solution: chicken anti-GFP (Abcam; ab13970; 1:500), rabbit anti-GFP (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2024Quote: ... Incubation with the primary antibody was carried out at 4°C overnight in a PBS (1x) solution containing anti-Ki67 (Abcam, MA, 1:100), BSA (1%) ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour at room temperature followed by overnight incubation at 4°C with primary antibody against TRP2/DCT (1:200, Abcam, catalog number ab74073). Alexa fluor-647 labeled secondary antibody (1:300 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1000 µg of sonicated chromatin was then used per immunoprecipitation reaction and were incubated with 4 µg of H3K27ac antibody (4729, Abcam, Cambridge, MA, USA). Immunoprecipitated complexes were washed ...
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bioRxiv - Microbiology 2022Quote: ... neutrophils on coverslips were fixed with 4% paraformaldehyde (PFA) and staining was performed with antibodies specific for H3Cit (citrulline R2 + R8 + R17, Abcam Ab5103, 1:200), myeloperoxidase (MPO ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skimmed milk for 2 h at room temperature and then incubated with primary antibodies overnight at 4 °C as follows: anti-collagen I (ab270993; Abcam, USA; 1:1000), anti-α-smooth muscle actin (α-SMA ...
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bioRxiv - Molecular Biology 2022Quote: ... Plates were then incubated overnight at 4°C with primary antibodies (rabbit anti-IRF3 antibody [Cell Signaling Technology 11904S]; rabbit anti-G3BP1 antibody [Abcam ab181149]) diluted in PBS + 0.1% Triton X-100 (Sigma-Aldrich X100 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at room temperature and then were incubated overnight at 4°C with the anti-MGST3 antibody (Abcam, ab192254; 1: 1000). Subsequently it was incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (Cell signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... Chondroitin sulfate (CS) and chondroitin 4-sulfate in the samples were determined following immunoprecipitation with CS antibody (CS-56, Abcam, Waltham, MA, USA), or C4S antibody LY111 (TCI Chemicals ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed and blocked cover slips were subsequently incubated overnight at 4° C with a rabbit anti-collagen I (Abcam ab260043, 1:250 dilution) primary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated either at room temperature for 2 hours or overnight at 4°C with primary antibodies in 5% milk in 1X TTBS at the following concentrations: DNAJB6 (Abcam #ab198995; 1:1000), CTCF (Cell Signaling Technology #2899 ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were blocked in 1% BSA in PBS for 30 minutes at room temperature before overnight incubation at 4°C in primary antibodies: α-mouse CENP-A (1:1000 Abcam ab13939 (discontinued) or 1:1500 Enzo ADI-KAM-CC006-E ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight: Anti-FMRP antibody (Abcam, ab17722, 1:1,000), anti-GAPDH antibody (Cell signaling technology ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked with Odyssey blocking buffer (Li-COR) and immunoblotted overnight (4°C) with rodent OXPHOS cocktail monoclonal antibody (Abcam; ab110413, 1:1000) to detect ETC proteins ...
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bioRxiv - Neuroscience 2023Quote: ... The slides with cryosectioned tissue were washed with PBS followed by incubation in blocking solution (2% of BSA in PBS) for 1 h and then incubated overnight at 4°C with an anti-DBI antibody (rabbit, 1:1000, Abcam, Cat. No: ab231910). Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000 ...
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bioRxiv - Pathology 2024Quote: ... The membrane was then probed overnight at 4°C with the primary antibodies (p105/p50, Abcam ab32360, 1:1000; Actin, Abcam ab3280, 1:1000) in Odyssey Blocking Buffer and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were blocked in 5% skim milk diluted in PBS-T for 1 hr at room temperature and incubated overnight at 4°C with a rabbit synaptophysin primary antibody (YE269, Abcam, Cambridge, UK; #ab32127) diluted 1:2,000 in blocking solution ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21: ab88572, Abcam; anti-GFP: ab290, Abcam). Paired-end sequencing was performed on a NovaSeq6000 platform (Illumina ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then incubated overnight at 4°C with the following primary antibodies in 2% w/v BSA: Rabbit anti-Gclc antibody (Abcam, United Kingdom; 1:1000); rabbit anti-NQO1 antibody (Cell Signalling ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP, 1:1000, Abcam; Ki67, 1:1000, ProteinTech). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... mice were fixed with 4% paraformaldehyde and the brain was sectioned at 200 μm thickness and immunostained against GFP (Abcam, no. ab13970, 1:300). Confocal microscopy (Zeiss LSM 700 confocal microscope ...
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bioRxiv - Physiology 2021Quote: ... Pre-synaptic motoneuron terminals were then labeled by incubating bundles overnight at 4°C in the same blocking solution to which had been added a mouse anti-synaptophysin antibody (AB8049 Abcam, USA; 1:25 dilution). The following morning bundles were washed (5 x 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in 5% milk TBS-T and incubated with blots at 4°C overnight (BMAL1, Abcam – ab93806; ACTIN, Abcam – ab3280, P84, Genetex – GTX70220). Blots were incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate ...
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bioRxiv - Physiology 2022Quote: ... 0,05% Tween 20 (TBS-t) for 60 min at RT and incubated overnight at 4°C with anti-IL6 antibody (Abcam, ab229381, 1:1000 dilution). Following 3 washes (10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then treated with 3% bovine serum albumin in PBS and incubated overnight at 4°C with the primary mouse antibody anti-Pax8 (Abcam ab53490, diluted 1/100). After washing unbound antibodies ...
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bioRxiv - Microbiology 2020Quote: ... before probing overnight at 4°C with antibodies raised to the following targets: goat polyclonal antibody to influenza A virus (ab20841, Abcam Inc., Toronto, ON, Canada), Mouse anti influenza A NA 1 (GeneTex ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4◻C in varying mixtures of the following primary antibodies: rabbit anti-DARPP-32 (1:1000; Abcam Cat# ab40801, RRID:AB_731843), mouse anti-Hu (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubations were performed for 24 hours at 4°C using an anti-glial fibrillary acidic protein (GFAP; Abcam, ab53554; 1:250 dilution) antibody ...
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bioRxiv - Microbiology 2020Quote: ... The rest of the chromatin was incubated overnight at 4°C with 1 μg of polyclonal anti-GFP antibody (Abcam ChIP grade, Abcam 290) or ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam, Cambridge, MA, USA), PD-L1 (ABclonal ...
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bioRxiv - Pathology 2020Quote: ... Immunostaining was performed by incubating different sections overnight at 4 °C using the following antibodies: human COMP (Abcam Cambridge, MA ab11056-rat 1:100), pS6 (1:200 2215S rabbit polyclonal Cell Signaling Technology) ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked in 5% skim milk in TBS + 0.1% Tween 20 (TBS-T) for 1 hour at room temperature before incubation in primary antibody overnight at 4°C (rabbit-α-caspase-1, Abcam #ab179515, 1:1000). Membranes were washed in TBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C: chicken anti-βIII tubulin (Tuj-1, 1:200; Abcam #ab41489; Boston, MA, USA), rabbit anti-PSD95 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... the fixed tissue sections were reacted individually overnight at 4°C with the following primary antibodies: rabbit anti-CD68 antibody (1:500, Abcam, Cat. #125212, Cambridge, MA), rabbit anti-CD146 antibody (1:500 ...
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bioRxiv - Genetics 2022Quote: ... for 1 hour at 4°C and then incubated overnight at 4°C with 1:1 mix of protein A/G Dynabead magnetic beads conjugated to H3K9me3 antibody (Abcam, cat# 8898 RRID: AB_306848).
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bioRxiv - Neuroscience 2024Quote: ... while the lower half was probed at 4 °C overnight with a 1:1000 dilution of a rabbit anti-β actin antibody (ab8227, Abcam, Waltham, MA, USA), in antibody dilution buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP; Abcam, ab3613; SantaCruz, sc-515550) or ...
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bioRxiv - Neuroscience 2023Quote: ... Following primary antibody incubation brains were washed 4 times for 15 minutes each in a 3% salt solution (in 1X PBST) and transferred to secondary antibodies (Abcam: ab175673; ThermoFisher: A-21236) in 1XPBST and incubated overnight at 4°C on a nutator ...
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bioRxiv - Molecular Biology 2023Quote: ... in methanol and non-specific binding blocked with 10% serum from the source species for the secondary antibody followed by a primary antibody incubation overnight at 4 °C (Sox9, abcam, ab185966,; p16, (abcam, ab241543), α-SMA (Sigma-Aldrich’ A5691) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated overnight in primary antibody in the blocking solution at 4°C using 1 or more of the following antibodies: α-SMA antibody (ab7817, 1:500, Abcam, Cambridge, MA, USA), FAP antibody (ab53066 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2% Tween 20) with 5% milk and incubated overnight at 4 °C with the primary antibodies: rabbit monoclonal anti-histone H2B (Abcam Cat#ab188291, 1:2000) or mouse monoclonal anti-beta actin (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained overnight at 4°C in blocking solution with the following antibodies and dilutions: Alexa Fluor® 488 anti-S100 beta (1:1000, Abcam, #ab196442, clone EP1576Y), PE anti-Nestin (1:150 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then incubated for 16 h O/N at 4°C with the following primary antibodies: CASR (Abcam Cat# ab79038, RRID:AB_2071489, 1:500), MAP2 (Abcam Cat# ab92434 ...
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bioRxiv - Biochemistry 2024Quote: ... After blocking 25 µL was removed and 25 µL of primary antibody solution in 1% BSA and 0.3% Triton-X100 in PEM was added to stain the cell body and flagella overnight at 4°C (anti-tubulin, Abcam #ab6161, 1:1000 dilution). Due to the amount of time required for imaging ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were added at 4 ºC overnight (Rabbit anti Lysozyme, Thermo Fisher Scientific PA129680, 1:50; Rabbit anti laminin, Abcam ab11575, 1:100). Samples were washed 5 x 1 hours with PBS at 4 ºC ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody incubation was performed overnight at 4°C using the following antibodies: anti-GFP rabbit (Abcam, diluted 1:2.000 in 5% milk in TBST) and anti-actin (Sigma ...