Labshake search
Citations for abcam :
1551 - 1600 of 1718 citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Membranes were blocked for 30 min with 3% BSA dissolved in Tris-buffered saline with 0.1% Tween (BSA-TBST) and incubated overnight at 4 °C with primary antibody (ab43177 chicken-developed anti-ATP synthase β, Abcam plc) diluted 1:5000 in BSA-TBST ...
-
bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 24 hours at 4°C in a primary antibody solution containing rat anti-BrdU (catalogue no. ab6326; Abcam, Cambridge, UK) diluted 1:500 in 0.3% Triton-X and 3% NDS in 0.1M PBS ...
-
bioRxiv - Immunology 2020Quote: ... The sonicated nuclear fractions were divided for input control and for overnight incubation at 4℃ with 10 μg of either anti-C/EBPβ (Abcam cat. 32358) or the negative control IgG ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary antibodies diluted in 1% NGS blocking buffer were added to the cultured cells overnight at 4°C (Oct4, 1:100, Santa Cruz; β-III-tubulin 1:500, Covance; TH, 1:1000, Chemicon; Serotonin, 1:500, Abcam). Negative primary controls consisted of cells treated with blocking buffer without the addition of primary antibodies ...
-
bioRxiv - Immunology 2020Quote: ... T cells were cultured with 2-deoxy-2-((7-nitro-2, 1, 3-benzooxadiazol-4-yl)amino)-glucose (2-NBDG) (Abcam 50μM, 1h), 4 ...
-
bioRxiv - Neuroscience 2020Quote: ... The retinas were incubated in PBS with 1% Triton X-100 and 0.5% Tween 20 for 1 h at room temperature and in 4% BSA for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies: RBPMS (1:500, Abcam, Cambridge, UK), ChAT (1:100 ...
-
bioRxiv - Physiology 2022Quote: ... 4 µm serial sections of paraffin-embedded slides were incubated with rat anti-mouse CD34 (MEC 14.7, monoclonal, Abcam, Cambridge, United Kingdom) at a 1:200 dilution overnight at 4° ...
-
bioRxiv - Genetics 2022Quote: ... After 6 h the cells were washed in 1XPBS and fixed in 4% PFA followed by staining with rabbit polyclonal beta COP antibody (ab2899, Abcam, Cambridge, UK) after permeabilising with 0.5% Triton-X 100 ...
-
bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
-
bioRxiv - Immunology 2022Quote: ... for 30 minutes at 4°C and then incubated with the titrated primary anti-MPO-PE and primary citrullinated histone H3 (citH3) antibody (Abcam, Waltham, MA) for 30 minutes at 4° C ...
-
bioRxiv - Physiology 2022Quote: ... Membranes were incubated in the following primary antibodies overnight at 4°C: polyclonal rabbit anti-beta actin IgG (Abcam Cat# ab8227, RRID:AB_2305186) at a dilution of 1:1,000 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... sections were incubated overnight at 4°C in 1:100 dilution of rabbit polyclonal anti-nitro tyrosine antibody (ab42789; Abcam, Cambridge, MA). Slides were rinsed in PBS and developed using polyclonal goat anti-rabbit Immunoglobulins conjugated with horseradish peroxidase (HRP ...
-
bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
-
bioRxiv - Cell Biology 2019Quote: Cells were fixed 48 h post transfection with 4% paraformaldehyde (PFA) and co-stained with rabbit anti-HA (1:500) and Mouse anti-CD63 (1:200; Abcam, Cambridge, MA) primary antibodies and with Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse (1:500 ...
-
bioRxiv - Neuroscience 2019Quote: ... dried skimmed milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with primary antibodies against NDUFS4 (Abcam, mouse, 1:500), NSE (Dako ...
-
bioRxiv - Neuroscience 2019Quote: ... GluN receptors were blocked by adding 4 μl of 25 mM d,l-2-amino-5-phosphonovaleric acid (APV; Abcam Cat# ab144498) to the 1 ml of aCSF in the interface recording chamber ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 24 hr in blocking buffer containing a chicken anti-mCherry antibody (1:500, ab205402, Abcam, Cambridge, MA). After PBS washes ...
-
bioRxiv - Cell Biology 2019Quote: ... the tissue was permeabilized with 2 washes of PBS0.5T (PBS + 0,5% v/v Triton X-100) for 1h and the samples were incubated overnight at 4°C with primary antibody against Keratin 14 (Abcam, 1:500) diluted in PBD0.2T (PBS + 1%BSA + 1% v/v DMSO + 0.2% v/v Triton X-100) ...
-
bioRxiv - Genetics 2021Quote: ... embryos were washed 3 times 10 minutes in 1x PBS with 0.1% Triton-X100 and 0.1% BSA and incubated overnight at 4°C with the secondary antibody goat-anti-mouse A568 1:500 (Abcam, antibody ID# ab175473). On the 4th day ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated on an orbital shaker for 24h at room temperature + 24h at 4 °C in polyclonal rabbit anti-mCherry (1:30 000, Abcam, cat. ab67453) or polyclonal chicken anti-EYFP (1:200 000 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... the sections were incubated overnight (16 h) in a humid chamber at 4°C with either rabbit SHH antibody (2 μg/ml; ab19897; Abcam, Cambridge, UK), mouse Col2 antibody (1/50 dilution ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
-
bioRxiv - Cell Biology 2021Quote: Cell supernatant was collected 24h after changing cell medium then immediately filtered by centrifugation for 15 minutes at 4°C on a 10KDa column (Abcam or Millipore). This step eliminates proteins ...
-
Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: Pull-down experiments were performed by following the method previously described.36 15-20 μg of acid extracted histones or nucleosomes isolated from HeLa cells were mixed with 50 μM of recombinant His6-tagged-BRPF2 bromodomain incubated at 4°C on a nutator for 60 min in the presence or absence of 10 μM BAY- 299 (abcam cat# ab230368) inhibitor ...
-
bioRxiv - Cancer Biology 2022Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with the following primary antibodies: KLF4 (R&D, AF3640) and cyclophilin B (abcam, catalog ab16045). Chemiluminescent detection was performed with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc. ...
-
bioRxiv - Cell Biology 2022Quote: ... The membrane was washed in TBST and incubated for 45 min in TBST containing 4% dried milk and HRP mouse anti-beta Actin AC-15 (1:25000, abcam Ref. ab49900). The membrane was washed in TBST and incubated 1 min in ECL Western Blotting Substrate (Thermo Fisher Scientific Ref ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12 ...
-
bioRxiv - Microbiology 2022Quote: ... Mitochondrial membrane potential was measured using a tetramethylrhodamine ethyl ester (TMRE) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) as a control (Abcam, Cambridge, UK). Prior to incubation and acquisition ...
-
bioRxiv - Neuroscience 2022Quote: ... retinas were incubated overnight at 4°C in the same solution containing a rabbit-anti-choline acetyltransferase (ChAT) monoclonal antibody (1:1000, Abcam ab178850, RRID:AB_2721842) and a guinea pig-anti-NeuN polyclonal antibody (1:500 ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; GAPDH ...
-
bioRxiv - Physiology 2022Quote: ... primary antibodies were added in 50 μL blocking buffer for 12 hours at 4° C: ATP synthase antibody (Abcam, ab128743 1:200), sAC antibody (R21 IHC ...
-
bioRxiv - Physiology 2022Quote: ... washed with PBS-T 3 times for 10min and incubated O/N at 4°C with primary antibody (Rabbit anti-Calnexin, Abcam, Cat# ab133615) at a dilution of 1:1000 in PBS-T ...
-
bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
-
bioRxiv - Cell Biology 2022Quote: ... Sections were then incubated in the following primary antisera overnight at 4°C in PBT: guinea pig antiinsulin (Abcam; Waltham, MA, USA), rabbit anti-glucagon (Santa Cruz ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with primary antibodies at the following dilutions: rabbit anti-MAP2 (ab254264 Abcam, 1:2000), mouse anti-βTUBULIN III (TUJ1 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated overnight at 4°C with anti-TOMM20 (1:200 dilution) and anti-cytochrome C (1:200 dilution) primary antibodies (Abcam, Waltham, MA). Thereafter ...
-
bioRxiv - Pathology 2024Quote: ... Membranes were then blocked for 1 hour with 5% dry milk in PBST (PBS supplemented with 0.1% Tween-20) buffer and incubated overnight at 4°C with primary antibody (anti-ACE2, Abcam; anti-Actin, Abcam) diluted in PBST containing 1% BSA (Sigma) ...
-
bioRxiv - Immunology 2024Quote: ... and incubated overnight at 4°C with the anti-F4/80 (#ab6640, 1:50) and/or anti-iNOS (#ab3523, 1:20) antibodies from Abcam (Cambridge, UK), and/or anti-AMPK alpha (Phospho Thr172 ...
-
bioRxiv - Neuroscience 2023Quote: ... The tissue was incubated for 22-24 h at 4 °C in a solution of primary antibody (1:2000 rabbit polyclonal to c-Fos, Abcam, Cambridge, MA) with 5% normal goat serum (Abcam ...
-
bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were incubated ON at 4°C with the following primary antibodies in blocking solution: rabbit-anti-DYRK1A (Abcam, 1:100), mouse anti-DYRK1A (Abnova ...
-
bioRxiv - Cell Biology 2023Quote: ... Sections were incubated overnight at 4°C with rabbit monoclonal anti-syntaxin 2 antibody (7.8 µg/ml; Abcam, Cambridge, England, UK: ab170852) or goat polyclonal anti- SNAP23 antibody (1.25 µg/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... The embedding blocks were cut on a cryostat into 20 μm thick sections and blocked for 30 min with 4% BSA and then incubated for 1 hour with the following primary antibodies: polyclonal rabbit anti-RFP (1:200, Abcam, Cambridge, UK).
-
bioRxiv - Pathology 2023Quote: ... membranes were incubated overnight at 4 °C with agitation with primary antibodies against acetylcholinesterase (68 kDa, 1:500, ab97299; Abcam, Cambridge, UK), butyrylcholinesterase (75 kDa ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were incubated with primary antibodies diluted in blocking buffer overnight at 4°C - Mouse Anti-Human TRA1-81 (1:75, Abcam, AB16289#20), Rabbit Anti-Human OCT4 (1:250 ...
-
bioRxiv - Cell Biology 2023Quote: ... LiCor) for 1 hour at room temperature and probed overnight at 4℃ with a monoclonal rabbit anti-HTT antibody (Abcam: EPR5526; Ab_109115) diluted 1:1,000 in blocking buffer plus 0.2% tween ...
-
bioRxiv - Immunology 2023Quote: ... H3Cit staining of fixed surface labelled cells was performed by incubating the cells overnight at 4°C in PBS 2% HI-FBS + 2mM EDTA + 0.1% saponin with monoclonal anti-H3Cit antibody (Abcam, ab219407, 1:600). Cells were washed and then incubated with donkey anti-rabbit AF555 (Invitrogen ...
-
bioRxiv - Genetics 2023Quote: Slides were incubated overnight at 4°C with the following antibodies diluted in PBS: rabbit anti-SYCP3 (ab15093, Abcam, 1:200 dilution), rabbit anti-SYCP1 (ab15087 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 48-72 hours at 4°C in primary antibodies: anti-Choline acetyltransferase 1:300 (Abcam catalog #: ab178850; RRID:AB_2721842), anti-Tyrosine hydroxylase 1:300 (Abcam catalog # ...
-
bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated at 4°C overnight with a monoclonal antibody to human cytokeratin antigen (Abcam, Cat #ab53280, at 1:250), or monoclonal antibody to human POSTN (R&D Systems ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in a humid chamber overnight at 4°C in primary antibody: anti-GFAP (Abcam [ab4674], 1:200 dilution), anti-Iba1 (Abcam [ab178846] ...