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Citations for abcam :
1551 - 1600 of 3615 citations for Acetamide N 5 bis 2 hydroxyethyl amino 2 2 chloro 4 6 dinitrophenyl azo 4 methoxyphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline (NBQX, 5 μM; Abcam, ab120046) to block excitatory glutamatergic activity in slices ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibodies were applied for 1-2 hours at room temperature and are as follows: Goat α-Rabbit-HRP (Abcam, ab97051, 1:5000), Donkey α-Rabbit-HRP (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the sections were blocked in 10% goat serum containing 1% BSA for 2 h at RT and sequentially incubated with primary antibodies: γ-H2AX (ab22551, Abcam; 1:100) or Ki67 (GTX16667 ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were incubated for 2 h in room temperature with the adequate HRP-conjugated secondary antibody: goat anti-rabbit IgG (1:5000, Abcam, GB; cat. 97051) or goat anti-mouse IgG (1:10000 ...
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bioRxiv - Bioengineering 2022Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was removed into a LoBind tube carefully so as not to remove any beads and 2 µg of anti-GFP antibody (Abcam ab290, anti-rabbit) were added to the sample and incubated overnight at 4°C with rotation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... e cells were then incubated with primary antibodies against mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) (1:200)(ab48508, 1:200 dilution; Abcam, Cambridge, United Kingdom) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunostaining was performed with the following antibodies at 1:50–500 dilutions in blocking solution for 1–2 hours at room temperature: anti-DDDD-K (Abcam, Cambridge, UK, ab1162), anti-GFP (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500) and ionized calcium binding adaptor molecule 1 (Abcam; ab5076; 1:50) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Trisbuffered saline (TBS) ...
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bioRxiv - Physiology 2020Quote: ... diluted in PBTD was added for 2 hours followed by an anti-elastin rabbit polyclonal antibody diluted in PBTD 1:100 (Abcam, Cambridge, UK; ab21610). The samples were incubated with the primary antibody for 18 hours at 4°C followed by 30 minutes washing in PBTD ...
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bioRxiv - Cell Biology 2021Quote: ... ∼ 5×1010 EVs collected from the two steps of purification were diluted into 500 μl of PBS and 2 μg of rabbit monoclonal anti-CD9 (Abcam, Cambridge, U.K., ab92726) or rabbit IgG (Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Physiology 2021Quote: ... 4µm sections of paraffin-embedded mouse kidneys were submitted to antigen retrieval for 20 min at 95°C in citrate buffer followed by incubation with Ly-6B.2 antibody (Abcam, ab53457, 1:100). Sections were incubated with HRP-labeled secondary antibody (Vector ...
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bioRxiv - Immunology 2020Quote: The HSV-seropositivity status of donors completing the survey was confirmed by ELISA using the anti-herpes simplex virus type 1 and 2 IgG human ELISA kit (abcam, Cambridge, United Kingdom) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553). Each experiment was repeated with similar results using anti-SARS-CoV-2 N rabbit polyclonal Ab (GeneTex # GTX135357 ...
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bioRxiv - Genomics 2022Quote: ... The cells were washed three times with PBS and resuspended with 2% BSA-PBS containing a goat anti-rabbit IgG FITC conjugated secondary antibody (Abcam catalog no. ab6717) and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated first with a blocking solution (BSA 1%, NGS 2%, Triton X-100 0.1%) and then with primary antibodies for KIF1A (Abcam, #ab180153, 1:100, rabbit), HTT (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Media was changed 2 h post-infection and the cells were fixed and immunostained 48 h post-infection to detect firefly luciferase (Abcam #ab181640, RRID # AB_2889835).
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bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Cancer Biology 2024Quote: ... The epithelial nature of the primary cultures and single-cell clones were analysed using a recombinant anti-EpCAM antibody (EPR20533-63; Catalogue number: ab221552, Abcam; Supplementary Figure 2).
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bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...
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bioRxiv - Immunology 2023Quote: ... cells that were stained with anti-AMPKα1 antibody and unconjugated isotype control antibody were incubated in the dark at room temperature for 2 hours with anti-rabbit Alexa Fluor 488 secondary antibody (Abcam cat. no. ab150113). Following antibody incubation ...
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bioRxiv - Pathology 2024Quote: ... IHC protocol modified from a previously described protocol(37, 38) using a rabbit anti-SARS-CoV-2 S protein antibody (Abcam, ab272504, Cambridge, UK) at 1:5000 dilution (0.2 µg/mL) ...
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bioRxiv - Bioengineering 2024Quote: ... Then the membrane was washed three times in TBS-T and incubated at room temperature for 2 hours with the following secondary antibodies goat anti-rabbit IgG H&L (HRP) (Abcam #ab205718, 1:2000) and m-IgG Fc BP-HRP (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell lysates of SH-SY5Y cells (10 µg) were subjected to Western blotting using the following antibodies: mouse anti-SLP-2 antibody (Abcam ab89025, 1:1000), rabbit anti-HSP60 (Cell Signaling #4870 ...
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bioRxiv - Physiology 2024Quote: ... and 0.3% Triton X-100 in PBS for 2 h at room temperature and incubated with primary c-FOS antibody (rabbit, Abcam ab190289, 1:1000) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... midguts were incubated at room temperature for 2 hours on an orbital shaker with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] at 1:4000) diluted in midgut blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-N-cadherin (N-cad) antibody (1:8000; ab76011; Abcam), and anti-β-actin antibody (1:20000 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated overnight with the primary antibody solution at 4 °C (blocking buffer solution contained primary antibodies with the following dilution: ch α GFP 1:2000, Abcam; rb α βIV-spectrin 1:1000, self-made 4). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... interleukin 6 (IL-6, Abcam#ab229434) and growth differentiation factor 15 (GDF15 ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were incubated overnight at 4°C with either anti-HA (Abcam, ab9110), anti-PTEN (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C with anti-MDA antibody (Abcam, ab6463) 1:250 in blocking buffer (PBS ...
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bioRxiv - Genetics 2021Quote: ... Then immunoprecipitation was performed overnight at 4 °C by incubating H3K27ac antibody (Abcam) precoated on protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated at 4°C overnight with anti-H4K8ac (ab15823, Abcam), anti-FTT-2 ...
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bioRxiv - Neuroscience 2020Quote: ... then overnight at 4°C with rabbit anti-Smo (1:500; ab38686; Abcam) or rabbit anti-caspase-3 cleaved (1:500 ...
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bioRxiv - Biochemistry 2020Quote: ... Oligomycin and carbonyl cyanide-4- (trifluoromethoxy)phenylhydrazone (FCCP) came from Abcam (Cambridge, UK). Carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Cell Biology 2021Quote: ... and incubation overnight at 4°C with primary antibodies (rabbit anti-H3K9me3, Abcam, ab8898 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 4-20% TEO-Tricine gradient gel (Abcam, Cambridge, MA) and transferred to a PVDF Immobilon membrane (EMD Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C (chicken anti-GFP 1:1000, cat. no. ab13970, Abcam; rabbit anti-c-Fos 1:800 ...
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bioRxiv - Pathology 2022Quote: ... followed by incubation overnight at 4 °C with Elastin (Abcam 21607 1:50) antibodies in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... and Neutrophil Elastase (1:100, Abcam, raised in rabbit, 4°C over night). Visualization of Neutrophil Elastase was done using an anti-rabbit-488 antibody (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... prior to overnight incubation at 4°C with a Rpl10a antibody (Abcam, ab174318). Alexa Fluor 594 conjugated secondary antibody (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibody incubation was incubated overnight at 4°C (Type IV tubulin, Abcam ab11315 ...
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bioRxiv - Biophysics 2021Quote: ... The permeabilized cells were incubated with primary antibody anti-4-HNE (Abcam 48506) diluted 1/200 in PBS buffer containing 5% BSA overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... incubated while shaking ON at 4°C with rabbit anti-Histone H3(Abcam) antibody used as a control ...