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Citations for abcam :
1351 - 1400 of 3615 citations for Acetamide N 5 bis 2 hydroxyethyl amino 2 2 chloro 4 6 dinitrophenyl azo 4 methoxyphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the conditioned media was given a short spin at 1000 rcf for 2 minutes to remove the cell debris and subjected to ELISA (ab108813, Abcam) to estimate the amount of APOE secreted by iPSCs.
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bioRxiv - Biochemistry 2021Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), CENP-E (mouse ...
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bioRxiv - Biochemistry 2021Quote: ... Invitrogen #PA5-66715, 1:500; Fumarase, Invitrogen #PA5-82899, 1:500; Casein Kinase 2 beta, Abcam #ab151784, 1:500; PDHAl [9H9AF5], Abcam #ab110330 ...
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bioRxiv - Developmental Biology 2021Quote: ... mice were bred to Rosa26-mTmG (91) and analyzed at 2 months by immunostaining with chicken anti-GFP antibodies (Abcam) and Alexa Fluor 647 hydrazide ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in 2% BSA in PBS to block against non-specific binding and incubated in 2% BSA containing rabbit anti-LC3B primary antibody (Abcam). Coverslips were then washed 3 × 5 minutes in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown and protein expressed in 2xYT media induced with the addition of 1 mM IPTG and 2 mM nhpSer (DL-AP4, Abcam) at 37 °C for 18 h.
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bioRxiv - Bioengineering 2021Quote: ... the medium was replaced with 200 μL DMEM and then incubated with a mixture of 2 μL Apopxin Green (Abcam) and 0.4 μL Propidium Iodide (15 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were subsequently subjected to 2 h of incubation with the appropriate corresponding horseradish peroxidase-conjugated secondary antibody (Abcam). Immunoblots were visualized by enhanced chemiluminescence (ECL kit ...
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bioRxiv - Cell Biology 2022Quote: ... Laminin alpha-2 was used specifically for costaining and was detected by Alexa Fluor 594 Donkey anti-rat (AB150156; 1:200; Abcam). All sections were mounted in Vectashield (Vector Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... The apical side of Caco-2 cells grown on Transwell inserts were exposed for 3 h to 20 nM Axl inhibitor (R428) (Abcam), 1 μg of anti-Gas6 antibody (R&D Systems) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed in 1xPBS with 0.05% Triton and incubated for 2 hours at room temperature with a secondary antibody (Abcam, ab150077). Brain slices were washed and mounted in Fluoromount (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: EA.hy926 cells or EZH2 WT/mutant overexpressing HEK-293 treated with NO donor SNP (500 µM) for 2 hours were processed with the help of a Biotin Switch Assay kit (Abcam) using the standard manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 h and then for 2 h at room temperature with the fluorescent secondary antibodies Alexa-555 donkey anti-rabbit (1:1000; Abcam), Alexa-633 donkey anti-mouse (1:250 ...
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bioRxiv - Microbiology 2024Quote: ... followed by a 2 hours incubation with respective Horseradish peroxidase-coupled secondary antibodies (anti-mouse IgG ab97023 and anti-rabbit IgG ab97051, Abcam). Luminescence was then detected using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at room temperature and then blocked in a solution of 0.3% PBS-T and 5% normal donkey serum (NDS) for 2 hours before being incubated with primary antibodies (1°Abs included: chicken anti-GFP [1:1000, Abcam, ab13970] ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were separated on a magnetic rack and washed once before being resuspended in 100 µl antibody buffer (wash buffer, 0.25% Digitonin and 2 mM EDTA) and 1 µl BRG1 antibody (Abcam, ab110641). Beads were incubated with the antibody over night with gentle shaking at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times with TBST and incubated with HRP-conjugated goat anti-human IgG (Fab’)2 (Abcam) or HRP-conjugated anti-rabbit IgG (Cell Signaling ...
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bioRxiv - Genomics 2024Quote: ... These strains bearing single-copy integrations were grown overnight at 30°C in rich medium YEA and used for ChIP with 2 µl of anti-GFP (ab290, Abcam). PCR oligonucleotides detecting central core (cc1&3 ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated for 2 h at room temperature with anti-rabbit IgG Alexa Fluor 488 (150081; Abcam, Boston, USA) and anti-mouse IgG Alexa Fluor 546 (Invitrogen A1003 ...
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Caspase cleavage of Influenza A virus M2 disrupts M2-LC3 interaction and regulates virion productionbioRxiv - Microbiology 2024Quote: Antibodies used in this study were mouse monoclonal anti-IAV Matrix 2 (Abcam, ab5416, WB 1:1000, IF 1:100), rabbit monoclonal anti-LC3B (Novus Biologicals ...
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bioRxiv - Microbiology 2022Quote: ... Cells were blocked with 2% BSA in PBS and then incubated with anti- H3Cit antibody (citrulline R2 + R8 + R17, clone EPR20358-120; Abcam) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and then incubated with 2% donkey serum followed by anti-human xanthine oxidase (1:1000; ab133268, Abcam, UK; RRID: AB_11154903), for 30min at 21 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The two fractions were split to two for IgG and YTHDF1 IP (2 μ each, rabbit IgG isotype control, Abcam ab37415 ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated with the following primary antibodies diluted in blocking buffer at room temperature for 2 hours: anti-OCT4 (1:200, catalog no. ab181557; Abcam); anti-TFAP2C (1:100 ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107 ...
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bioRxiv - Cell Biology 2023Quote: ... Blocking was performed with 2% BSA in PBS for 30 min and cells were incubated with phalloidin-iFluor 594 reagent (1:1000; ab176757; Abcam) for 1 hour.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were blocked with goat serum for 2 hours at room temperature and incubated with RAD51(1:500 no. ab133534 Abcam) or yH2AX (1:200 no ...
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bioRxiv - Neuroscience 2023Quote: ... were washed with PBS + 0.1% Tween-20 (PBS-T) then incubated with 2 µL of PDI antibody (Abcam, Catalog #ab2792) in 200 µL PBS-T for 10 min (end-over-end rotation ...
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bioRxiv - Biophysics 2023Quote: Equimolar mixture of histone octamer and LIN28B DNA (2 µM each) were mixed with 1 µM of Oct4 (abcam ab134876) and 3 µM of Sox2 (50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The medium in each well was replaced with HBSS with 10 μM DCF-DA (2’,7’-dichlorofluorescein diacetate; D6883, Abcam) or propidium iodide staining solution (P4864 ...
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bioRxiv - Developmental Biology 2023Quote: EdU (5-ethynyl-2′-deoxyuridine) labeling assay was performed according to the instruction of EdU Assay / EdU Staining Proliferation Kit (ab219801, Abcam). Proliferating cells were labeled by injecting 10mg/kg BW EdU to mice intraperitoneally 2 hours before sacrifice ...
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bioRxiv - Developmental Biology 2023Quote: ... BSA (blocking agent) was applied on slides for 35 minutes followed by overnight incubation with primary antibodies [MMP-2 (Abcam) at a dilution of 1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), BUBR1 (rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in PBS and incubated for 2 hr at room temperature with secondary antibodies 594-donkey-anti-rabbit (1:50; Abcam) and 488-donkey-anti-chicken (1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230). Final cDNA Libraries were prepared from twice immunoprecipitated RNA and fragmented input total RNA using the Smarter® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio 634411 ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of antibodies ChIP-grade (H3K4me2, Abcam ab32356; H3K4me3, Diagenode C15410003; H3, Abcam ab1791; Normal Rabbit IgG, Abcam ab37415) were used per ChIP and were bound to DiaMag protein G-coated magnetic beads (Diagenode ...
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 5% skim milk for 2 h at room temperature then incubated with primary antibodies (Rabbit monoclonal to ATPB at 1/50000 dilution, Abcam) for 16 h at 4 ℃ ...
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bioRxiv - Cell Biology 2023Quote: ... ab199956), Bcl-2 antibody (1:1000, rabbit anti, ab194583), and gp91phox (1:2500, rabbit anti, ab129068) were purchased from Abcam company ...
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bioRxiv - Developmental Biology 2023Quote: The following antibodies were used for chromatin immunoprecipitation: rabbit anti-NK Homeobox 2-1 (NKX2-1, 1 µg per reaction, ab133737, Abcam) and rabbit anti-CEBPA (C/EBPα ...
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bioRxiv - Bioengineering 2024Quote: The second subset of islets was evaluated for oxidative stress by 2’,7’-Dichlorodihydrofluorescein diacetate (DCFDA) (Abcam, cat. no. ab113851) staining of reactive oxygen species (ROS) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... the sections were incubated with primary antibodies SOX 2 Mouse (Sta. Cruz) with a dilution of 1:200 and Ki-67 rabbit (Abcam) with a dilution of 1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody solution for Aldh1l1 and vimentin staining: 2 mL blocking buffer containing 20 μL anti-Aldh1l1 antibody (Abcam, AB56777) and 20 μL anti-vimentin (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at room temperature and then blocked in a solution of 0.3% PBS-T and 5% normal donkey serum (NDS) for 2 hours before being incubated with primary antibodies (1°Abs included: chicken anti-GFP [1:1000, Abcam, ab13970] ...
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bioRxiv - Neuroscience 2024Quote: ... Patient IgG4 was detected using 25 ng/mL of an HRP-conjugated goat F(ab’)2 anti-human IgG-Fc polyclonal antibody (Abcam) in assay buffer for one hour at room temperature followed by adding TMB substrate ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 488-conjugated anti-chicken secondary antibody (goat, 2 mg/ml, dilution 1:500; catalog no. ab150169, Abcam) were used for GFlamp2 and ChR2-EYFP.
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bioRxiv - Bioengineering 2024Quote: ... The primary antibodies and respective concentrations used in this study are the following: anti-Ki67 (2 µg/mL, ab15580, abcam), anti-p21Cip1/Waf1 (0.48 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 h and immediately incubated with a rabbit anti-c-Fos antibody (1:2,000; ab190289, abcam, Cambridge, UK; RRID:AB_2737414) at RT overnight (16 h) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the dissected intestines were incubated for 2 hours at 25°C in antibodies against GFP (Abcam, Waltham, MA; Cat#: ab6556) and nuclear pore complex (NPC ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 1-2 h at room temperature with the indicated primary antibodies: rabbit anti-lamin B1 (ab16048, Abcam), mouse anti-separase (6h6 ...