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Citations for abcam :
1401 - 1450 of 10000+ citations for 1 Piperidineacetamide 4 2 benzothiazolyl N 4 4 morpholinyl phenyl 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Membranes were incubated in the following primary antibodies overnight at 4°C: polyclonal rabbit anti-beta actin IgG (Abcam Cat# ab8227, RRID:AB_2305186) at a dilution of 1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: Cell supernatant was collected 24h after changing cell medium then immediately filtered by centrifugation for 15 minutes at 4°C on a 10KDa column (Abcam or Millipore). This step eliminates proteins ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: Pull-down experiments were performed by following the method previously described.36 15-20 μg of acid extracted histones or nucleosomes isolated from HeLa cells were mixed with 50 μM of recombinant His6-tagged-BRPF2 bromodomain incubated at 4°C on a nutator for 60 min in the presence or absence of 10 μM BAY- 299 (abcam cat# ab230368) inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with the following primary antibodies: KLF4 (R&D, AF3640) and cyclophilin B (abcam, catalog ab16045). Chemiluminescent detection was performed with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12 ...
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bioRxiv - Microbiology 2022Quote: ... Mitochondrial membrane potential was measured using a tetramethylrhodamine ethyl ester (TMRE) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) as a control (Abcam, Cambridge, UK). Prior to incubation and acquisition ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then incubated in the following primary antisera overnight at 4°C in PBT: guinea pig antiinsulin (Abcam; Waltham, MA, USA), rabbit anti-glucagon (Santa Cruz ...
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bioRxiv - Pathology 2024Quote: ... Membranes were then blocked for 1 hour with 5% dry milk in PBST (PBS supplemented with 0.1% Tween-20) buffer and incubated overnight at 4°C with primary antibody (anti-ACE2, Abcam; anti-Actin, Abcam) diluted in PBST containing 1% BSA (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were fixed in 4% paraformaldehyde for 15 min and stained with anti-γ-H2AX (CST,9718) and chicken anti-GFP (Abcam, #ab13970) primary antibodies in 1:400 and 1:600 dilution ...
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bioRxiv - Microbiology 2023Quote: ... bound complexes were isolated by overnight precipitation of the pre-cleared chromatin with 100 µL of blocked beads in combination with 3-4 µg of antibodies (anti-Histone H3 (Abcam ab17913, µg), Histone H3Ac (Millipore 17-615 ...
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bioRxiv - Biophysics 2024Quote: ... and then were blocked with the 10% normal goat serum for 45 minutes at room temperature before incubating with the primary antibodies (anti-γH2AX: Beyotime, no. C2035S-4; anti-pSTING: Cell Signaling Technology, no. 40818S; anti-cytochrome c and anti-complex V: Abcam, no ...
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bioRxiv - Cell Biology 2024Quote: ... in PBST for two hours followed by overnight incubation (4 °C) with anti-DNA/RNA damage antibody [15A3] (Abcam, cat. No. ab62623) at 1:2500 in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... and 4 μg of the specific antibody (rabbit anti-Rpb1, Cell Signaling, Cat.14958; rabbit anti-RNA Pol II phospho S2, Abcam, ab5095) was incubated in IP buffer (0,1% SDS ...
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bioRxiv - Bioengineering 2024Quote: ... Extracellular glutamate was measured in effluents collected at the end of TNFα administration (day 4) using the same kit used for the glutamate measurements in the bicuculline experiment (Abcam, Cat.# ab83389). Chips were then fixed for immunostaining and confocal microscopy ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBS-T) and incubated overnight at 4℃ with primary antibodies against NDUFA9 (Abcam, Cat. No. ab14713), MTCO2 (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... were diluted in blocking buffer and incubated on tissue sections at 4°C for 16h (Abcam, Cambridge, UK; Bioss USA, Woburn, MA). Tissue sections were washed with 0.05% Tween 20 in PBS ...
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bioRxiv - Physiology 2020Quote: ... and anti-N-Cadherin (1:300, Abcam #ab18203). Secondary antibodies included goat anti-mouse Alexa Fluor 488 (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-N-cadherin (rabbit, 1:350, Abcam (ab18203)) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-N-CADHERIN (ab18203 Abcam, 1:50), rabbit anti-TBR1 (20932-1-AP Proteintech ...
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bioRxiv - Neuroscience 2020Quote: ... anti-p62 1:1000 (Abcam, Cat. n° Ab56416), anti-polyUbiquitin 1:5000 (Santa Cruz ...
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bioRxiv - Neuroscience 2020Quote: ... anti-IGF2 1:1000 (Abcam, Cat. n° Ab9574), anti-LC3 1:1000 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-HECTD1 (N-terminus) (Abcam, Ab101992, 1:200), Anti-HA (Roche ...
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bioRxiv - Bioengineering 2021Quote: ... anti-N protein antibodies (1:500, ab7164, Abcam), anti-GFP antibodies (1:3000 ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit-α-N-Cadherin (abcam, ab76011, 1/250). Secondary antibodies used were goat-α-rabbit-488 (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... anti-N mouse (ab94806 – Abcam, 1:200 IF), anti-P mouse (ab94965 – Abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-N-Cadherin (ab18203; Abcam; 1:200), rabbit anti-Sox2 (ab92494 ...
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bioRxiv - Developmental Biology 2024Quote: ... N-Cadherin (1:100, Cat. No. AB18203, Abcam); and HuC/D (1:100 ...
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bioRxiv - Genetics 2021Quote: ... ran side by side in a Criterion Precast Gradient 4-15% SDS-PAGE gel for western blotting probing of key intracellular organellar markers and proteins of interest: HSP60 (Abcam, cat. number ab46798), Calnexin (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at room temperature and were incubated overnight at 4 °C with primary rabbit polyclonal antibodies against mouse MAGL (Abcam, Cambridge, UK; ab180016) or human MAGL (LSBio ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were incubated in blocking buffer containing (TBST+5% BSA) for 1hour at RT and incubated with primary antibody in blocking buffer at 4°C overnight: POLD1 (Abcam Cat# ab168827,1:500); GAPDH (Santa Cruz Biotechnology Cat# sc-32233 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were incubated at 4°C overnight with the following primary antibodies: α- GADD153/CHOP (SCBT sc-166682) and α-Tubulin (Abcam ab176560). After washing ...
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bioRxiv - Pathology 2020Quote: ... membranes were probed against anti-citH3 or anti-PAD4 or anti-MPO or anti-p-ERK antibodies overnight at 4°C according to the manufacturer’s instructions (Abcam, UK and Massachusetts, USA). Membranes were washed extensively (4x ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in goat serum for 30 min and incubated overnight at 4°C with primary antibodies against DOT1L (Abcam, ab64077, 6 µg/ml), HIF-1α (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... 1000 µg of sonicated chromatin was then used per immunoprecipitation reaction and were incubated with 4 µg of H3K27ac antibody (4729, Abcam, Cambridge, MA, USA). Immunoprecipitated complexes were washed ...
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bioRxiv - Molecular Biology 2022Quote: ... Plates were then incubated overnight at 4°C with primary antibodies (rabbit anti-IRF3 antibody [Cell Signaling Technology 11904S]; rabbit anti-G3BP1 antibody [Abcam ab181149]) diluted in PBS + 0.1% Triton X-100 (Sigma-Aldrich X100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Chondroitin sulfate (CS) and chondroitin 4-sulfate in the samples were determined following immunoprecipitation with CS antibody (CS-56, Abcam, Waltham, MA, USA), or C4S antibody LY111 (TCI Chemicals ...
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bioRxiv - Biochemistry 2024Quote: ... A 2-hour blocking in 3% w/v non-fat milk in PBST was followed by overnight rocking at 4 °C with primary antibodies (anti-His-Abcam, Rabbit polyclonal (Ab137839) and anti-TULP3-Abcam ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 1:4000 fold diluted rabbit anti-SARS-CoV-2 N protein-specific IgG (Abcam, Cat.#ab271180) at room temperature for 1 h ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21: ab88572, Abcam; anti-GFP: ab290, Abcam). Paired-end sequencing was performed on a NovaSeq6000 platform (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in 5% milk TBS-T and incubated with blots at 4°C overnight (BMAL1, Abcam – ab93806; ACTIN, Abcam – ab3280, P84, Genetex – GTX70220). Blots were incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate ...
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bioRxiv - Microbiology 2020Quote: ... before probing overnight at 4°C with antibodies raised to the following targets: goat polyclonal antibody to influenza A virus (ab20841, Abcam Inc., Toronto, ON, Canada), Mouse anti influenza A NA 1 (GeneTex ...
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bioRxiv - Neuroscience 2023Quote: ... Following primary antibody incubation brains were washed 4 times for 15 minutes each in a 3% salt solution (in 1X PBST) and transferred to secondary antibodies (Abcam: ab175673; ThermoFisher: A-21236) in 1XPBST and incubated overnight at 4°C on a nutator ...
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bioRxiv - Molecular Biology 2023Quote: ... in methanol and non-specific binding blocked with 10% serum from the source species for the secondary antibody followed by a primary antibody incubation overnight at 4 °C (Sox9, abcam, ab185966,; p16, (abcam, ab241543), α-SMA (Sigma-Aldrich’ A5691) ...
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bioRxiv - Physiology 2024Quote: ... Colonic sections were then incubated with primary antibodies overnight at 4°C followed by secondary antibodies (mouse IgG ab150113 Abcam, rabbit IgG ab96891 Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... p21 N’-terminus (Cat # ab80633, CP74, Abcam, 1:500), RRM1 (Cat # CST3388 ...
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bioRxiv - Developmental Biology 2020Quote: ... Rabbit anti-N-cadherin at 1:50 (Abcam, ab211116) and Mouse anti-ZO-1 antibody at 1:100 (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... Anti N-terminal αSyn (Abcam, ab51252, 1:1000 dilution), C-terminal αSyn (Santa Cruz ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-N Cadherin antibody (ab18203, Abcam; 1:100), rabbit anti-Rec8 antibody43 (a gift from Lampson Lab) ...
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bioRxiv - Cell Biology 2024Quote: ... Rabbit anti-Dclk1 (1:1000 O/N, Abcam ab31704), Rabbit anti-ChgA (requires prefixation ...
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bioRxiv - Cell Biology 2024Quote: ... Rabbit anti-Muc2 (1:1000 O/N, Abcam ab272692), Rabbit anti-Dclk1 (1:1000 O/N ...