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Citations for abcam :
1301 - 1350 of 10000+ citations for 1 Piperidineacetamide 4 2 benzothiazolyl N 4 4 morpholinyl phenyl 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... were sonicated and incubated overnight under rotation at 4 °C with 5 ug/ml of anti-RNAPII-Ser2p (Abcam, ab193468) or anti-IgG (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... Sections were then washed in deionized water and blocked with a solution of 5% normal goat serum (NGS) in PBS for 15 min at 37 °C and then stained overnight at 4 °C with 4G2 antibody (Abcam) at 1:200 ...
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bioRxiv - Molecular Biology 2024Quote: Cells were stained 3-4 days after editing with anti-Cytokeratin 5 antibody [EP1601Y] (Alexa Fluor® 647) (Abcam, ab193895), Rabbit IgG ...
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bioRxiv - Molecular Biology 2024Quote: ... Debris were removed using centrifugation and the chromatin was incubated overnight at 4°C with antibody against H3K9me3 (Abcam, Ab8898). Antibody-bound chromatin was captured using magnetic beads (Themo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% BSA pH 7.4 in phosphate buffered saline (PBS) or Tris-HCl buffered saline (TBS) and incubated at 4°C overnight with primary antibodies (anti-Nr4a2, Abcam, ab41917 ...
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bioRxiv - Immunology 2023Quote: ... the specimens were incubated overnight at 4 °C with 5µg/mL rabbit anti-GFP antibody (ab290, Abcam, Cambridge, MA, USA). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... Following imaging for loading control the membrane was blocked in 5% BSA in TBS-T and incubated overnight at 4 °C using N6-methyladenosine antibody (ab151230, Abcam). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... Where antibody staining was required coverslips were permeabilized with 0.2% Triton X-100 for 10 min and blocked in 4% BSA in PBS for 20 min followed by incubation with relevant antibodies (DHRS7 abcam at 1:1,000 dilution or rabbit lamin A antibodies [17] at 1:50 dilution for 1 hr at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue sections were then stained overnight at 4°C with primary antibodies against anti-CD3 (ab52959, Abcam, CA, USA), anti-CD4 (AF-379-NA ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated overnight at 4°C on a shaker (65 rpm) with primary antibodies as follows: anti-CD206 (Abcam) for macrophages and anti-CD31 (Dako ...
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bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
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bioRxiv - Developmental Biology 2022Quote: ... peak incident power 105 W) and incubated overnight at 4° with magnetic beads conjugated to the H3K27ac antibody (Abcam, ab4729). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... for 30 min followed by overnight incubation at 4° in primary antibodies, Aβ (Novus, NBP11 -97929) and NRN1 (Abcam, ab64186). Membranes were washed with 1×Tris-buffered saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Physiology 2022Quote: ... permeabilized and saturated for 30 minutes at room temperature in PBS with 0.01% Triton-X PBS and 4% donkey serum and incubated with chicken anti-GFAP (Abcam, ab4674) and rabbit anti-S100β (Dako ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained in blocking buffer overnight at 4°C with the following primary antibodies at the indicated dilutions: anti-Gal-9 (Abcam ab275877 ...
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bioRxiv - Immunology 2023Quote: The following primary antibodies were used for these experiments: rabbit anti-4 hydroxynonenal (HNE) antibody (10 µg/ml; ab46545, Abcam), rat anti-cd11b antibody (0.5 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... and rabbit anti-titin M-line (Myomedix) primary antibodies overnight at 4°C then incubated in goat anti-mouse AF488 (Abcam) and goat anti-rabbit AF647 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent and incubated overnight at 4 °C (Collagen I (# ab34710, Abcam, Collagen IV (#ab6586, Abcam), MUC-1 (MA1-06503 ...
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bioRxiv - Immunology 2024Quote: ... in blocking buffer ON at 4°C and then incubated with the secondary anti-rabbit Alexa 594 (Abcam, Cambridge, UK) and anti-mouse Alexa 488 (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... Pooled chromatin was incubated at 4°C for 12−14 h with rotation with 2.5 µg anti-H3K27ac (Abcam, ab4729) or 2.5 µg anti-H3K4me3 (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were post-fixed on slides with 4% paraformaldehyde before being permeabilized with 3% TritonX-100 and immunostained with IBA1 (AbCam), NeuN (ThermoFischer) ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated overnight at 4 °C in the blocking solution with primary antibody (Iba1: rabbit anti-Iba1, ab178846 Abcam, 1:200 dilution ...
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bioRxiv - Physiology 2024Quote: ... Colonic sections were then incubated with primary antibodies overnight at 4°C followed by secondary antibodies (mouse IgG ab150113 Abcam, rabbit IgG ab96891 Abcam) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Paraffin-embedded tissue sections of 4 µm thick from the distal colon were processed following the manufacturer’s protocol for the TUNEL assay kit (Abcam, #ab206386). Images of 10 randomly selected fields per slide were captured at 400X magnification ...
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bioRxiv - Cell Biology 2024Quote: ... followed by an overnight incubation at 4°C with primary antibodies specific for Osx and Cx43 both anti-rabbit (Abcam, Cambridge ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used for cell staining overnight at 4°C: anti-alpha smooth muscle actin [1A4] antibody (Abcam) and isotype control mouse IgG2a (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in primary antibody overnight at 4° C (chick α-GFP, Abcam, Cat# AB13970, Lot# GF305729-1; mouse α- γ-tubulin, Sigma-Aldrich, Cat# T6557, Lot# 066M4858V; rabbit α- α- tubulin, Abcam, Cat# AB233661) and in secondary antibody for two hours at room temperature (Alexa goat α-chick 488 ...
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bioRxiv - Cancer Biology 2021Quote: ... carried out overnight on a rotating wheel at 4 °C in the presence of 20 μg of anti-V5 antibody (Abcam, ab9116). 2.5% of the input sample was collected just before addition of the antibody and stored at −20 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated at 4°C with one of the following mouse monoclonal primary antibodies: anti-heavy chain cardiac myosin (Abcam, #ab50967), anti-α-actinin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... at 4 °C overnight followed by staining with a secondary goat-anti-rabbit antibody coupled with Alexa 568 (ab175471, Abcam, US).
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21: ab88572, Abcam; anti-GFP: ab290, Abcam). Paired-end sequencing was performed on a NovaSeq6000 platform (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Sections from animals not injected with FITC-Dextran were next incubated under stirring for 48 hours at 4°C in PB containing rabbit polyclonal anti-collagen IV antibody (Abcam, Ab6586) at a 1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were resolved by SDS-PAGE using a 4-15% gradient gel and analyzed by Western blot using the following primary antibodies: rabbit anti-GFP (Abcam Ab290), rabbit anti-Cx34.1 3A4-conjugated-680LT ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887] ...
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bioRxiv - Cell Biology 2021Quote: ... Afterwards membranes were blocked for 30 min with 5% Nutrilon (Nutricia) in PBS and incubated overnight at 4°C with primary antibodies H3 (Abcam 1791), H4 (Merck Millipore 04-858) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were incubated overnight at 4 °C with recombinant anti-vimentin antibody conjugated with Alexa Fluor® 568 (ab202504, Abcam®) at a 1/1000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were blocked for 1 hour at room temperature with 5% fat-free milk or milk-free blocking buffer (for analysis of phosphorylated proteins) and incubated overnight at 4°C with primary antibodies for p-GCN2 (Abcam, ab75836), p-eIF2α (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... slides were thoroughly washed using PBS 1X/Triton- X 0.1% and incubated overnight at 4°C with the respective antibodies anti-mouse Alexa-Fluor® 594 (abcam, ab150108), anti-sheep Alexa-Fluor® 488 (Jackson Immunoresearch ...
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bioRxiv - Immunology 2022Quote: ... and CTLA-4 Ig (Abatacept) were generated using the APC Conjugation Kit - Lightning-Link® according to manufacturer’s instructions (Abcam, ab201807). APC conjugated anti-PD-L1 antibodies were procured from Biolegend (clones MIH1 ...
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bioRxiv - Microbiology 2020Quote: ... endogenous human NPC1 and HSP90 were purified using immobilized Recombinant Protein G Resin (Generon) and 4 µg of specific antibodies against NPC1 (Abcam, ab108921) or HSP90 (Enzo Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were resolved by SDS-PAGE using a 4–15% gradient gel and analyzed by Western blot using the following primary antibodies: chick anti-GFP (Abcam Ab13970), rabbit anti-Cx34.1 3A4-conjugated-680LT ...
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bioRxiv - Genetics 2020Quote: ... the membranes were then probed at 4°C overnight with various primary antibodies: anti-foxa2 (Seven Hills) or LXR-α (Abcam), washed with TBST (20 mM Tris ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight in a humidity chamber at 4°C in a mixture of rabbit anti-androgen receptor (Spring Bioscience m4070) and chicken anti-tyrosine hydroxylase (Abcam ab76442) in NGS/TBS/BSA ...
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bioRxiv - Biophysics 2020Quote: ... A negative control was performed on a subset of the wells by treating cells with a spike of 10 µM Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, Abcam ab120081) 10 minutes prior to the application of TMRE ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C in primary antibody diluted in blocking solution with 5% normal goat serum (Abcam, ab7481). Antibodies were directed against GFP (Abcam ...
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bioRxiv - Biochemistry 2023Quote: Rat sera were incubated at 4°C for 16 h in the presence of anti-adiponectin antibodies from Abcam (ab 56416) and Cell Signaling (2789s) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...