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Citations for abcam :
1001 - 1050 of 2615 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Visualized neurons were labeled for chicken ovalbumin upstream promoter transcription-factor interacting protein-2 (CTIP2, 1:1000, rat, Abcam, Cat#:ab14865, RRID:AB_2064130) and pre-proenkephalin (PPE ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C in blocking solution with 1% NDS and primary rat anti-BrdU (1:500, Abcam, Cambridge, UK). On the third day ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 24 hours at 4°C in a primary antibody solution containing rat anti-BrdU (catalogue no. ab6326; Abcam, Cambridge, UK) diluted 1:500 in 0.3% Triton-X and 3% NDS in 0.1M PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with secondary antibody for 1 hr at room temperature: rat-HRP 1:10,000 dilution (abcam ab205720 Lot# GR32115155-1), rabbit-HRP 1:10,000 dilution (Santacruz sc-2004 Lot#B2216) ...
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bioRxiv - Physiology 2022Quote: ... 4 µm serial sections of paraffin-embedded slides were incubated with rat anti-mouse CD34 (MEC 14.7, monoclonal, Abcam, Cambridge, United Kingdom) at a 1:200 dilution overnight at 4° ...
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bioRxiv - Bioengineering 2021Quote: ... the channels were incubated at 4 °C for 16 h with the following primary antibodies: anti-mouse laminin MEC5 (rat IgG; Catalog number ab2466; 1:50; Abcam, Cambridge, UK), anti-mouse collagen IV (rabbit IgG ...
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bioRxiv - Physiology 2020Quote: ... was incubated for 1 hour at RT followed by 1 h incubation with streptavidin-DyLight 647 (Vector, 1:300) and anti-rat DyLight 594 (Abcam, 1:300) and subsequently 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-ADMA (5,6, kind gift of Pr C. Haass, Munich Germany, 1/100) or rabbit anti-Ubi (Abcam, ab179434, 1/100). After 3 rinses in PBS ...
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bioRxiv - Physiology 2023Quote: ... Adjacent sections were used for immunohistochemistry with anti-VDR (1:100, rat) and anti-AMH (1:50, mouse) monoclonal antibodies (Abcam, Cambridge, UK) as per manufacturers’ protocol with modifications.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed twice with PBST before being incubated with the primary antibody (Mouse anti-BrdU, 1:1000, Cat. No. B35128, Invitrogen; or Rat anti-BrdU, 1:1000, Cat. No. ab6326, Abcam, RRID: AB_305426) and the secondary antibody (Donkey anti-mouse 488 or 568 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: Choline and acetylcholine were quantified in rat cortical tissue from all experimental groups using a choline/acetylcholine assay kit (ab65345, Abcam, Cambrige, UK). 10 mg of cortical fresh tissue were harvested ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Life Technologies a11006, Donkey anti Rabbit 647 Abcam ab150075,). Slides were again washed 3 times 5 minutes each with PBS-T ...
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bioRxiv - Neuroscience 2019Quote: ... with 0.3% Triton X for one hour before being staining in in PBS with 0.3% Triton X with rat anti-BrdU (Abcam: 6326 at 1:200), and mouse anti-NeuN (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the sections were incubated with histofine simple stain kit Rat MAX-PO(G) (414331F, Nichirei, Tokyo, Japan) or goat anti-chicken IgY conjugated biotin (ab6876, abcam plc, Cambridge, UK) for 30 minutrs at RT ...
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bioRxiv - Cell Biology 2019Quote: ... SAA and perilipin were performed using a rat anti-mouse F4/80+ antibody and rabbit anti-mouse perilipin (both 1:100 dilution, Abcam®, Cambridge, UK), and a rabbit anti-mouse SAA (1:200 dilution ...
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bioRxiv - Physiology 2020Quote: ... for 1 h followed by a 72 h incubation with primary antibodies as appropriate: rabbit anti-IBA1 at 1:1000 (Wako, 019-19741, Madison, WI) and rat anti-TMEM119 at 1:250 (abcam 209064, Cambridge, UK) [24 ...
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bioRxiv - Cell Biology 2021Quote: ... The primary antibodies rat monoclonal anti-CD13 (Novus, NB100-64843, 1:500) and rabbit monoclonal anti-Sox9 (Abcam, Cat. No. ab185966, 1:500) were diluted in Tx buffer (0.2% gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... and the last sample was added with 1 μL of anti-c-Myc rat IgG1 (1 mg/mL; clone JAC6, Abcam #ab10910, RRID: AB_297569). The antibody binding was performed for 2–3 h at 4°C on a rotator ...
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bioRxiv - Genetics 2019Quote: ... Slides were immunostained overnight at 4°C or 4h at 30°Cwith a mixture of the two primary antisera diluted in blocking buffer [rat anti-tubulin (Abcam ab6160 1:1250) and rabbit anti-MYC (Santa Cruz ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA fibers were incubated with mouse-FITC-anti-bromodeoxyuridine (detects IdU, B44, Becton Dickinson, 1:50) and rat-anti-bromodeoxyuridine (detects CldU, abcam ab6326, 1:100), at 37°C for 1 hour and then with anti-mouse IgG AlexaFluor 488 (Invitrogen A11029 ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated at room temperature for 1 hour with mouse monoclonal primary antibodies against rat substance P (1:300 dilution, ab14184, Abcam, Cambridge, MA, USA) or normal mouse serum (ab7486 ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT: goat anti-rat IgG at 1:2000 (Abcam, Waltham, MA #ab97057), sheep anti-mouse IgG at 1:2000 (GE Cytiva #NA931) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times for 10 min with 1 ml of PBS and then incubated with 500 µl of secondary antibody containing donkey anti-rat IgG Alexa Fluor 594 (ab150156, Abcam, diluted 1:300) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Cell Biology 2020Quote: ... CldU- and IdU-labeled tracks were detected by 1 hr incubation with rat anti-BrdU antibody (1:400, AbD Serotec; 1:1000, Abcam; detects BrdU and CldU) and mouse anti-BrdU antibody (1:400 ...
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bioRxiv - Pathology 2020Quote: ... Immunostaining was performed by incubating different sections overnight at 4 °C using the following antibodies: human COMP (Abcam Cambridge, MA ab11056-rat 1:100), pS6 (1:200 2215S rabbit polyclonal Cell Signaling Technology) ...
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bioRxiv - Cell Biology 2021Quote: ... Rat antibodies against the following proteins were used: α18 (gift from Professor A. Nagafuchi, Nara Medical University, Japan; 1:50) and rat E-Cadherin (Abcam 13-1800; 1:500). Goat antibody against p114 RhoGEF was purchased from Sapphire Bioscience (EB06163 ...
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bioRxiv - Molecular Biology 2022Quote: ... The CldU and IdU tracks were detected by IF with anti BrdU antibodies (rat for CldU, Abcam ab6326, and mouse for IdU, BD 347580). Fiber integrity was assessed with a mouse anti-ssDNA antibody (Millipore MAB3034) ...
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bioRxiv - Biochemistry 2022Quote: ... and re-probed (1h, RT, 1/10000 diluted in PBS-Tween with 5% non-fat milk) with rat anti-β actin (AbCam, Cambridge, UK, RRID: AB_867488) washed (as above ...
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Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzymebioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody (Jackson ImmunoResearch 109-605-003: Goat anti-huIgG conjugated to Alexa Fluor 647, 1:250, Abcam GL2A7: Rat anti-LAMP2, 1:500, Abcam EPR12763: Rabbit anti-NeuN, 1:500) prepared in Antibody Dilution Buffer (1%BSA/0.1% sodium azide in 1xPBS ...
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bioRxiv - Biophysics 2023Quote: ... The cleared supernatant was then incubated with 3 µg anti-myc antibody (SIGMA M4439 monoclonal anti c-myc or Abcam ab206486 rat mAb to myc tag (9E10)) for 1.5h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... cells were fractionated using cell Fractionation Kit (Abcam) and RNA extracted using TRI Reagent (Molecular Research Center ...
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bioRxiv - Cell Biology 2022Quote: Cells were harvested and processed using Cell Fractionation Kit (Abcam ab109719) following standard protocol with optimization for ARPE-19 cells ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were resuspended at 1 × 106 cells/mL in cell-culture media supplemented with 2 mM thymidine (Abcam ab143719) and incubated at 37 °C in 5% CO2 for 18 hours ...
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bioRxiv - Bioengineering 2020Quote: ... the cells were incubated with the Cytopainter cell membrane stain (Abcam, ab219941), washed three times with washing buffer ...
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bioRxiv - Microbiology 2019Quote: Cell viability was tested using an MTT Cell Proliferation assay kit (Abcam) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Cell viability was detected using the CytoPainter Live Cell Labeling Kit (Abcam) and used to normalize ROS and caspase activity ...
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bioRxiv - Immunology 2022Quote: ... red blood cells were removed by Red Blood Cell Lysis Buffer (Abcam). Cells were then washed twice with PBS containing 0.04% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... cells tested for viability using Cell Counting Reagent 8 (Abcam, Cat# ab228554). Briefly ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was determined by a Live and Dead Cell Assay (Abcam). After HaCaT cells were attached to the device for 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... NRF2 (Abcam, cell signaling), HPRT ...
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bioRxiv - Physiology 2020Quote: Cell viability was determined using an XTT cell proliferation assay kit (Abcam-ab232856) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell proliferation was assessed using Crystal violet Assay Kit (Cell viability) (ab232855, abcam).
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bioRxiv - Genetics 2021Quote: Cell proliferation rate was measured with a BrdU Cell Proliferation ELISA Kit (Abcam), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: Cell proliferation was assessed using the Abcam MTT Cell Proliferation Assay Kit (Abcam) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cell proliferation was analyzed with BrdU Cell Proliferation Elisa Kit (Abcam, Cambridge, UK). In brief ...
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bioRxiv - Cancer Biology 2021Quote: ... iii) Subcellular fractionation of the cells was performed (Cell Fractionation Kit, Standard, Abcam, ab109719) according to the manufacturers’ protocol ...
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bioRxiv - Bioengineering 2021Quote: Cell-cell junctions were stained using a rabbit anti-VE-cadherin primary antibody (Abcam). Actin filaments and nuclei were stained using Alexa Fluor phalloidin (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Cells with each ADAR isoform were fractionated with the Cell Fractionation Kit (Abcam: ab109719) according to the manufacturer’s instructions ...