Labshake search
Citations for abcam :
851 - 900 of 2615 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: ... The remaining lysates were equally divided and incubated with 7 μl IgG (negative control, CST) and FTO antibody (Abcam), respectively ...
-
bioRxiv - Microbiology 2021Quote: ACE2 enzymatic activity was performed on serum samples and tissue lysates using the ACE2 activity fluorometric kit (abcam ab273297) following manufacturer’s instructions using a SpectraMax M2 (Molecular Devices).
-
bioRxiv - Cancer Biology 2021Quote: ... lysates were diluted twofold in assay buffer and incubated with capture beads for FUT8 (protein G-agarose beads; Abcam) overnight ...
-
bioRxiv - Immunology 2020Quote: ... 50 mL lysates was kept as input and the rest were incubated with 5 μg RAD21 antibody (Abcam, #ab992) or 10 μg CTCF antibody (Millipore ...
-
bioRxiv - Pathology 2020Quote: ... The primary antibodies used for WBs prepared using murine liver lysates were rabbit anti-LDLR (1:5,000; Abcam ab52818) and mouse anti-β-actin (1:10,000 ...
-
bioRxiv - Genetics 2019Quote: ... the lysate was transferred to PVDF membranes and incubated with primary antibodies (anti-Mbnl1, abcam, ab108519; anti-SIX5, abcam, ab113064 ...
-
bioRxiv - Microbiology 2023Quote: ... Immunoblotting of RIPA lysates was performed as previously described79 with antibodies against IFNAR1 (Abcam, Cambridge UK, ab124764, 1:500), IFITM3 (Proteintech ...
-
bioRxiv - Cell Biology 2020Quote: ... The Alexa Fluor 488 (Donkey Anti-Rabbit and Anti-Rat IgG H&L) were also obtained from Abcam. The APC-alpha-6Itg anti human/mouse CD49f was obtained from BioLegendR ...
-
bioRxiv - Developmental Biology 2021Quote: ... Sections were stained with rat anti-Sox2 (eBiosciencce) and FITC-conjugated mouse anti-cTnT antibodies (Abcam, Cat#ab105439) (E9.5 only) ...
-
CD8+ T-cell-mediated immunoediting influences genomic evolution and immune evasion in murine gliomasbioRxiv - Cancer Biology 2019Quote: ... IHC was performed with a 1:150 diluted rat monoclonal antibody against MHC-I (AB15680) (Abcam; Cambridge, UK), 1:150 diluted rabbit polyclonal antibody against MHC-II (AB180779 ...
-
bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used at the indicated dilution: anti-tubulin (rat monoclonal, Abcam #ab6161; 1:200), anti-phospho-ERM (rabbit monoclonal ...
-
Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Mitochondria were isolated from adult rat brain by differential centrifugation using mitochondria isolation kit for tissue (ab110168, abcam). Western blot for alpha-synuclein was performed using 4–12% Bis-Tris gels (Life Technologies) ...
-
CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... 10% donkey serum and 0.5% NP40 in PBS and stained over night at 4°C with following primary antibodies in 2% BSA and 0.5% NP40 in PBS: rat anti-Ctip2 (1:1,000, Abcam), rabbit anti-Neurofilament200 (1:80 ...
-
bioRxiv - Microbiology 2021Quote: ... Primary immuno-detection of BrdU was with rat anti BrdU BU1/75 (ICR1) antibody (1:100 dilution, Abcam). Slides were washed in blocking solution three times and incubated with goat anti-rat Alexa 488 secondary antibody (1:500 dilution ...
-
bioRxiv - Cancer Biology 2022Quote: ... 347580)] for 110 minutes at 37°C followed by washing and secondary antibodies goat anti-rat Cy5 (Abcam, ab6565) and goat anti-mouse Cy3.5 (Abcam ...
-
bioRxiv - Biochemistry 2022Quote: ... serum samples were collected from each rat and were analyzed according to the following kits: methylglyoxal (Abcam ab238543), RAGE (Abcam ab202409) ...
-
bioRxiv - Neuroscience 2019Quote: ... rats were tested for their intake following treatment with saline or CCK-8 (4μg/kg IP; Abcam, ab120209) 30 minutes prior to being placed in the chamber ...
-
bioRxiv - Immunology 2021Quote: ... and then incubated with peroxidase-conjugated goat anti-mouse IgG or goat anti-rat IgG (Abcam, Cambridge, UK) for 1 hr ...
-
bioRxiv - Immunology 2021Quote: ... HRP-conjugated rat monoclonal [187.1] anti-mouse kappa light chain antibody (abcam ab99617, 1:1500 dilution in PBSM) and HRP-conjugated goat polyclonal anti-human IgG F(ab’)2 fragment antibodies (Jackson ImmunoResearch 109-036-008 ...
-
bioRxiv - Biophysics 2022Quote: ... before being incubated for 1.5h at room temperature with 1:500 rat anti alpha-tubulin antibodies (abcam ab6160) in 1% BSA diluted in PBS-0.2% Triton (BSA-PBST) ...
-
bioRxiv - Cell Biology 2023Quote: ... Plate-bound antibody to ATP synthase was co-incubated with 4μg of solubilized rat heart mitochondria (Abcam ab110347) in the presence or absence of recombinant human cTnI (2ug ...
-
bioRxiv - Cell Biology 2023Quote: ... and incubated as floating sections in primary antibody (Mouse monoclonal Anti-SOX2 SantaCruz Biotech sc-365823, RRID:AB_10842165; Rat monoclonal Anti-CTIP2 Abcam ab18465 ...
-
A spatially resolved EGFR signaling model predicts the length scale of GAB1-SHP2 complex persistencebioRxiv - Systems Biology 2023Quote: ... coverslips were incubated overnight at 4°C with rat EGFR antibody (ab231, 1:200; Abcam, Cambridge, United Kingdom). EGFR was detected by incubating coverslips for 1 hr at 37°C with anti-rat Alexa Fluor 594 (A-11007 ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by 4°C overnight incubation of the Cldu specific antibody (Rat anti-BrdU; 1:100, ab6326, Abcam), diluted in 5% goat serum in PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... after which they were incubated in primary antibody (mouse anti-MBP 1:500, Merck NE1019, RRID:AB_604550; rat anti-MBP 1:500, Abcam ab7349 ...
-
bioRxiv - Biochemistry 2019Quote: ... The beads were discarded and the lysate was rotated overnight at 4 °C in the presence of 9 μl anti-HA rabbit (ab9110, Abcam) or 9 μl anti-Rpn2 (PSMD1 ...
-
bioRxiv - Cancer Biology 2019Quote: ... 30 μg of protein lysate from each fraction was analyzed by western blotting using the anti-HER4 antibody E200 (Abcam) to determine HER4/4ICD subcellular localization ...
-
bioRxiv - Cancer Biology 2020Quote: ... Binding ELISA kit was performed according to the manufacturer’s protocol using nuclear lysates and values were normalized to μg protein as determined by protein estimation (Abcam, ab113474).
-
bioRxiv - Cell Biology 2021Quote: Aconitase activities in cytosolic and mitochondrial fraction of the brain cortex lysates were measured using Aconitase Enzyme Activity Microplate Assay Kit (Abcam) according to manufacturer’s instructions.
-
bioRxiv - Cell Biology 2021Quote: ... rabbit anti-SQSTM1/p62 (IP 1-2 μg/mg of lysate; ab101266) and rabbit anti-GFP (WB 1:2000, ab290) were from Abcam; mouse anti-SQSTM1/p62 (WB 1:1000 IF 1:200 ...
-
bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was incubated with 5 μl of ssDNA (10mg/ml) and 2 µl of mouse monoclonal anti-V5 antibody (Abcam), 4 µl of rabbit polyclonal anti-PrA (Sigma ...
-
bioRxiv - Genomics 2021Quote: ... Validation of Myc-tagged RARaa protein expression was done by western-blotting on cytosolic and nuclear lysate fractions from injected embryos using a ChIP-grade anti-myc antibody (ab9132, Abcam).
-
bioRxiv - Cancer Biology 2021Quote: ... Lysates were cleared by centrifugation (14,800 g, 20 min, 4°C) and subsequently incubated with anti-HOXA5 antibody (Abcam, #ab82645), anti-DDK (FLAG ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 25 μg of the crude lysates were subjected to SDS-PAGE and transferred onto nitrocellulose membrane before immunoblotting with RL2 (1:1000, ab2739, Abcam) or CTD110.6 (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... cellular lysates were incubated for 1h with LND or vehicle with was measured using a hexokinase assay kit (Abcam, ab211103) according to manufacturer instructions.
-
bioRxiv - Cell Biology 2023Quote: APOB protein levels in RPE and retinal lysates were measured using an Apo B ELISA Kit (ab230932, Abcam Cambridge, UK). Briefly ...
-
bioRxiv - Genetics 2024Quote: ... Protein G beads (80 uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting H3K18ac (Abcam #ab40888), H3K18pr (PTM Bio #PTM-213) ...
-
bioRxiv - Developmental Biology 2020Quote: Western blot for T1CM ESCs were performed as described previously113 using monoclonal antibody rat anti-TET1 5D6 (1:10)119 and polyclonal rabbit anti-H3 (1:5,000; ab1791, Abcam) as loading control ...
-
bioRxiv - Immunology 2019Quote: ... then incubated in secondary antibodies in 0.1% Triton X-100/PBS for 2 hr at 4°C (goat anti-rat Alexa 488, Abcam, ab150157 at 1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling ...
-
bioRxiv - Microbiology 2021Quote: ... Primary immuno-detection for BrdU was done with rat anti BrdU BU1/75 (ICR1) antibody (1:100 dilution, Abcam), together with mouse anti ssDNA (clone16-19 ...
-
bioRxiv - Cell Biology 2021Quote: ... The CldU and IdU tracts were labelled (for 2.5 hr in the dark, at RT) with anti-BrdU antibodies recognizing CldU (rat; Abcam) and IdU (mouse ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-Mouse IgG2b heavy chain (HRP) (ab97250, 1;5000), and anti-Rat IgG2a H&L (HRP) (ab106783, 1:5000) was bought from Abcam.
-
bioRxiv - Cancer Biology 2022Quote: Immunodetection of the replication tracks was performed using primary antibodies diluted in 1% BSA [Rat anti-CldU (Abcam, ab6326); mouse anti-IdU (BD Biosciences ...
-
bioRxiv - Neuroscience 2022Quote: ... parvalbumin (PV; mouse, SWANT: PV 235, RRID: AB_10000343; 1:2000) and Ctip2 (rat, Abcam: ab18465, RRID: AB_2064130; 1:500).
-
bioRxiv - Cell Biology 2022Quote: ... diluted in blocking buffer for one hour at RT: donkey anti-rat Alexa Fluor® 647 (1:250; abcam); donkey anti-goat Alexa Fluor® 568 (1 ...
-
bioRxiv - Pathology 2019Quote: ... blocked with 2.5% normal horse serum (Vector ImmPress) and 1:100 dilution of mouse anti-rat anti-CD68(ED1) antibody (Abcam) applied for 30 min followed by horse anti-mouse Polymer (rat absorbed ...
-
bioRxiv - Cell Biology 2020Quote: ... The FUrd signal was detected by staining with anti-BrdU [BU1/75 (ICR1)] Rat mAb (abcam, Boston, MA, ab6326) and Alexa Fluor 488 anti-Rat IgG (Jackson Labratory ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated with primary antibodies against IdU and CldU (rat anti-IdU monoclonal antibody [MAb] [1:150 dilution; Abcam] and mouse anti-CldU MAb [1:150 dilution ...
-
bioRxiv - Bioengineering 2019Quote: ... Primary antibodies were used as follows: rat anti-glial fibrillary acidic protein (GFAP, 1:100, Abcam, Cambridge, MA, USA) for astrocytes and rat anti-CD11b (1:100 ...