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Citations for abcam :
9151 - 9200 of 10000+ citations for Human IgM Anti Zika Virus NS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The brain sections of the rats with Gi-DREADDs were incubated first in a blocking solution (10% fetal horse serum and 0.15% Triton X-100 in PBS) for 1 h at 24 ± 1°C and then with primary rabbit anti-mCherry antibody (1:1000, Abcam, ab167453, Cambridge, UK) overnight at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed with PBS thrice and NP antigen was detected using a goat anti-influenza NP polyclonal antibody (abcam catalogue number ab155877) for 1 h ...
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bioRxiv - Plant Biology 2024Quote: ... Protein expression of rSPL9-GFP was verified by SDS-PAGE and immunoblotting using an anti-GFP antibody (Abcam, Cat# ab290, 1:10,000 dilution) and HRP-conjugated secondary antibody (Promega ...
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bioRxiv - Cancer Biology 2024Quote: Immunofluorescence (IF) was performed on whole FFPE 5-µm-thick tissue sections using anti-TP63 mouse monoclonal antibody (1:100; Abcam, catalog no. ab735), anti-CK8 rabbit polyclonal antibody (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells that were stained with anti-AMPKα1 antibody and unconjugated isotype control antibody were incubated in the dark at room temperature for 2 hours with anti-rabbit Alexa Fluor 488 secondary antibody (Abcam cat. no. ab150113). Following antibody incubation ...
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bioRxiv - Neuroscience 2024Quote: ... blocked for 1 hour with 10% serum in PBS and incubated overnight at 4LC with the following primary antibodies in PBS: chicken anti-MAP2 (Abcam, ab92434, 1:500), rabbit anti- tyrosine hydroxylase (TH ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were incubated with the following primary and secondary antibodies in PBS/10 % FBS for 1 hour to overnight: mouse anti-MRE11 (abcam, #ab214, 1:500), rabbit anti-TFAM (antibodies-online ...
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bioRxiv - Microbiology 2024Quote: ... Antigen-specific IgG antibodies in hamster sera were detected using horseradish peroxidase (HRP)-labeled goat anti-Syrian hamster IgG H+L Ab (Abcam, 1/30000 dilution). IgG1 and IgG2/3 subclass Abs were analyzed using biotinylated subclass-specific antibodies (Southern Biotech ...
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bioRxiv - Neuroscience 2024Quote: ... Tween-20 in tris-buffered saline (TBS-T) then incubated overnight at 4°C with one of the following primary antibodies: rabbit anti-GluK4 (Abcam; ab10101, 1:500), rabbit anti αB-crystallin (Enzo Life Sciences ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in blocking solution for 2 hr and then incubated with goat anti-rabbit-HRP secondary antibody (Abcam; ab6721; 1:300) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Midguts were washed five times via direct tube transfer in PBS+T before staining with the secondary antibody (Goat anti-mouse Alexa Fluor 594, Abcam, Cambridge, United Kingdom) at a 1:1000 dilution in blocking solution for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... The lungs were harvested and cryosections were prepared for immunofluorescence (IF) staining with an anti-BrdU antibody (Abcam, Cambridge, UK; Catalogue no. ab70328). Images were acquired and analysed using an Applied Spectral Bioimaging System (ASI ...
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bioRxiv - Cancer Biology 2024Quote: ... The epithelial nature of the primary cultures and single-cell clones were analysed using a recombinant anti-EpCAM antibody (EPR20533-63; Catalogue number: ab221552, Abcam; Supplementary Figure 2).
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bioRxiv - Microbiology 2024Quote: ... The equivalent of 0.3 OD units was analyzed by SDS-PAGE and western blot using the following antibodies and dilutions: mouse anti-AD (Abcam, cat nr ab135398) 1:200 in 3% BSA in PBS-0.5% Tween (PBS-T) ...
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bioRxiv - Cancer Biology 2024Quote: ... Formalin-fixed paraffin embedded tissues from 10 NSCLC patient-derived xenograft (PDX) models were processed for immunohistochemistry to detect centrosomes using the anti-pericentrin antibody ab4448 (Abcam, 1:1000 dilution). Mitotic cells were classified as having CA if they contained more than 2 centrosomes and the proportion with CA was calculated for each tumor ...
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bioRxiv - Bioengineering 2024Quote: ... Incubation with the primary antibody was carried out at 4°C overnight in a PBS (1x) solution containing anti-Ki67 (Abcam, MA, 1:100), BSA (1%) ...
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bioRxiv - Genomics 2023Quote: ... cells were immunostained in blocking buffer containing mouse anti-beta III Tubulin (Tub) conjugated Alexa Fluor 488 antibody (Abcam ab195879, dilution 1:1000) and DAPI (Sigma D9542 ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... while sections of caspase and protein S staining were incubated with goat anti-rabbit IgG secondary antibody conjugated with HRP (Abcam ab6721, 1:1,000). ImmPACT DAB Peroxidase (HRP ...
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bioRxiv - Genomics 2022Quote: ... The cells were washed three times with PBS and resuspended with 2% BSA-PBS containing a goat anti-rabbit IgG FITC conjugated secondary antibody (Abcam catalog no. ab6717) and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at room temperature and then were incubated overnight at 4°C with the anti-MGST3 antibody (Abcam, ab192254; 1: 1000). Subsequently it was incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (Cell signaling ...
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bioRxiv - Cell Biology 2023Quote: ... 1:400 dilution) and anti-α- synuclein aggregate antibody (Clone#: MJFR-14-6-4-2; 1:1000 dilution) were acquired from Abcam (Cambridge UK).
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skimmed milk for 2 h at room temperature and then incubated with primary antibodies overnight at 4 °C as follows: anti-collagen I (ab270993; Abcam, USA; 1:1000), anti-α-smooth muscle actin (α-SMA ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) labeled with Alexa Fluor®-647 (Alexa Fluor® 647 Conjugation Kit (Fast)-Lightning-Link® (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were then blocked using 5% non-fat dry milk (NFDM) for 45 minutes at room temperature and incubated with primary antibodies: anti-GFP (catalog number ab290; Abcam; dilution 1:5000) and anti-GAPDH (catalog number MA5-15738 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA detection was performed by applying 2 ng of the sample used for PicoGreen detection to a nylon membrane and detecting with the anti-dsDNA antibody (Abcam #ab27156, 1:9000).
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bioRxiv - Molecular Biology 2023Quote: ... Blocking buffer was discarded and slides were washed and incubated with primary antibody: rabbit anti-Alpha-synuclein (phospho S129) (Abcam #ab51253, 1:10000) diluted in blocking buffer overnight ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-Sc35 (MilliporeSigma, S4045, 1:1000) and incubated with the secondary antibodies using Alexa 488-labeled goat anti-mouse IgG (Abcam, Ab150113, 1:1000), Alexa 594-labeled goat anti-rabbit IgG (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... 7 μm thick frozen sections of ileal tissue were blocked in 10% NGS and stained with biotinylated anti-GFP antibody (Abcam, ab6658, 1:250) or anti-occludin AlexaFluor 594 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Primary IgG antibodies specific to Lamin B1 (#ab16048) and the secondary IgG donkey anti-rabbit AF405 conjugated antibody (#ab175651) were purchased from Abcam (San Francisco, A). The SNAP-Cell® TMR-Star and Oregon Green® were purchased from New England Biolabs (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... sections were mounted on slides and stained using a monoclonal mouse anti-muscarinic acetylcholine receptor M4/CHRM4 primary antibody (1:500, abcam, ab77956, RRID: AB_1566454), with blocking reagents and secondary anti-mouse biotinylated antibody from the M.O.M ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then permeabilized and blocked with the blocking buffer [0.1% Triton X-100 and 2% bovine serum albumin in phosphate buffered saline (PBS)] for 30 min and subsequently incubated with the primary antibodies rabbit anti-αTubulin (Abcam, ab18251,1:1000 dilution), mouse anti-acetylated Tubulin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
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bioRxiv - Immunology 2023Quote: ... and blocked in 2% heat-inactivated-FBS for 1 hour at room temperature followed by incubation with primary antibodies polyclonal rabbit anti-H3Cit (Abcam, ab5103, 1μg/mL) and goat anti-Myeloperoxidase (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-nucleocapsid (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed three times in PBST and incubated with secondary antibodies diluted in blocking solution for two hours at room temperature (RT): Goat anti-Rabbit conjugated Alexa Fluor 568 (Abcam #175471, 1:500) and 1:500 Goat anti-Chicken Alexa Fluor 488 (A11039 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT: goat anti-rat IgG at 1:2000 (Abcam, Waltham, MA #ab97057), sheep anti-mouse IgG at 1:2000 (GE Cytiva #NA931) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-H3 (A/Perth/16/2009 ...
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bioRxiv - Systems Biology 2023Quote: ... the cells underwent an additional 30-minute blocking step using 1× blocking buffer and were then incubated for 1 hour with secondary antibodies – Goat Anti-Mouse IgG H&L (Alexa Fluor® 594, Abcam, Cambridge, UK), and Phalloidin-iFluor 488 Reagent (Abcam ...
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bioRxiv - Microbiology 2023Quote: FFPE sections were evaluated by multiplex immunohistochemical analysis following previously reported protocol using antibodies specific for anti-α-SMA (ab124964, Abcam, 1:500), anti-IL-8/IL-8 (MAB208 ...
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bioRxiv - Biochemistry 2023Quote: ... for 10 min and blocked in 0.5% BSA in 0.5% Tx-PBS for 1 h at room temperature before incubation with mouse monoclonal anti-ψ-tubulin antibody (Abcam, ab11316, 1:1000) in 0.5% BSA in 0.5% Tx-PBS overnight at 4 ºC ...
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bioRxiv - Neuroscience 2023Quote: ... The slides with cryosectioned tissue were washed with PBS followed by incubation in blocking solution (2% of BSA in PBS) for 1 h and then incubated overnight at 4°C with an anti-DBI antibody (rabbit, 1:1000, Abcam, Cat. No: ab231910). Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...
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bioRxiv - Developmental Biology 2023Quote: ... was used as a positive control to confirm a successful experiment and a secondary antibody anti-Mouse IgG (Abcam, ab46540, 1:50 dilution) was used as negative control to remove background in the following sequencing analysis.
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bioRxiv - Developmental Biology 2023Quote: ... Fractions pellet was resuspended in SDS sample buffer and analyzed by Western blotting (WB) using anti-GFP antibody (Abcam, ab 290, 1:1,000). GFP positive fractions (5 and 6 ...
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315, Abcam, Cat.#ab6789, respectively). For HisTag purification ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was done for 1 h at RT with the following horseradish peroxidase (HRP)-coupled antibodies: HRP-conjugated goat anti-mouse IgG (Abcam Cat# ab6789, RRID:AB_955439), HRP-conjugated goat anti-rabbit IgG (Abcam Cat# ab6721 ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for western blotting at the indicated dilutions: anti-CD63 (MBL, D263-3, 1:5000; Abcam, ab134045, 1:2000), anti–Flotilin-1 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 3 times for 10 min with 1 ml of PBS and then incubated with 500 µl of secondary antibody containing donkey anti-rat IgG Alexa Fluor 594 (ab150156, Abcam, diluted 1:300) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... After blocking with 5% skim milk (Beyotime, China) and sequential incubation with the primary and secondary antibodies: anti-PRMT3 (Abcam, Ab191562, 1:2000), anti-PRMT3 (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... midguts were incubated at room temperature for 2 hours on an orbital shaker with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] at 1:4000) diluted in midgut blocking buffer ...