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Citations for abcam :
9101 - 9150 of 10000+ citations for Human IgM Anti Zika Virus NS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies: VCP (Abcam), and YFP (Roche) ...
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bioRxiv - Biophysics 2021Quote: ... the cells were blocked for 1 h at room temperature using an unconjugated goat anti-mouse IgG H&L antibody (polyclonal, 1:1000, Abcam ab6708, Cambridge, UK) diluted in previously mentioned blocking solution ...
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bioRxiv - Cancer Biology 2021Quote: ... with the following antibodies and conditions (dilution, heat-mediated antigen retrieval solution, retrieval time): anti-ERG (Abcam, clone EPR386, 1/100, H1, 30min); anti-p53 (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitation of α-tubulin interacting proteins was performed from total cell lysates using α-Tubulin specific antibodies (rabbit anti-α-tubulin, ab52899, Abcam) and Protein A/G Sepharose beads (Santa Cruz Biotechnology) ...
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used for brain sections (supplier, catalog number and working dilution are indicated): anti-GFP chicken polyclonal (Abcam ab13970, 1:2000); anti-dsRED rabbit polyclonal (Tanaka LivingColors 632496 ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used in this study were as follows: goat anti-rabbit IgG Alexa Fluor 555 (1:500 dilution; abcam; Cat. No. ab150078), goat anti-mouse IgG Alexa Fluor 488 (1:500 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then washed twice with PBS and resuspended in goat anti-rat IgG Alexa Flour 647 secondary antibody (Abcam, Cambridge, United Kingdom) diluted 1:1000 in Blocking Buffer and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed 3 times and then stained simultaneously with two secondary antibodies: goat anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1:400 diluition; ab150117; Abcam, Cambridge, UK) and rhodamine red -X-coniugated affinity pure goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were saturated with 10% non-fat milk in PBS and then probed with the following primary antibodies: Anti-Synaptophysin (Abcam, ab14692, 1:1000), Anti-FUS N-ter1 (ProteinTech ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was then incubated overnight on a shaker at 4 °C in blocking solution amended with the primary antibody [anti-Lamin B1 (Abcam; ab16048; 1:1000), anti-GAPDH (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... Protease Inhibitor) and antibodies (FLI 7.3 mouse, Santa Cruz; H3K27ac rabbit, Abcam ab4729; Rabbit anti-mouse IgG, Abcam ab46540; LSD1 rabbit, Abcam ab17721) were added at a dilution of 1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... so membranes were also incubated with a mouse monoclonal anti-beta actin primary antibody (dilution: 1:5000, product number: ab8226, Abcam, Cambridge, MA, USA). After TBST washing three times for 10 minutes each wash ...
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bioRxiv - Cancer Biology 2022Quote: ... The newly replicated CldU and IdU tracts were incubated with anti-BrdU antibodies recognizing CldU and IdU respectively (ab6326, Abcam; 347580, BD Biosciences) for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were incubated for 2 h in room temperature with the adequate HRP-conjugated secondary antibody: goat anti-rabbit IgG (1:5000, Abcam, GB; cat. 97051) or goat anti-mouse IgG (1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... To confirm that the Iba-1 antibody labeled resident microglia and not infiltrated macrophages we co-labeled the Iba-1 positive cells using goat anti Iba-1 antibody (Abcam ab5076, 1:125) with rabbit polyclonal antibody for rat TMEM119 that recognizes microglia-specific transmembrane proteins (Synaptic Systems GmbH ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies: mouse anti-HSP70 (ab2787, Abcam, Cambridge, MA, USA), rabbit anti-TSG101 (14497-1-AP ...
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bioRxiv - Bioengineering 2022Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min with primary antibodies diluted in blocking solution as follows: mouse anti-Pyruvate dehydrogenase E2/E3 (PDHE2/E3, ab110333, Abcam, 1 μgmL−1); mouse anti-clathrin heavy chain (X22 ...
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bioRxiv - Microbiology 2021Quote: ... the endothelial cells were incubated with anti-gC1qR antibodies (Santa Cruz Biotechnology, clone 74.5.2 Cat. # sc-23885 and Abcam, clone 60.11, Cat. # ab24733), anti-αvβ3 antibody (Millipore Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... in a Tris-glycine-methanol transfer buffer overnight and detected with the following antibodies: 1:5000 monoclonal mouse anti-Hsp70 [C92F3A-5] (Abcam Cat# ab47455, RRID:AB_881520) (abcam) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 infected cells were visualized using SARS-CoV-2 S protein specific immunochemistry staining with anti-SARS-CoV-2 spike glycoprotein S1 antibody (Abcam, Cambridge, Great Britain) as described previously [64] ...
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bioRxiv - Neuroscience 2021Quote: ... Co-labeling for astrocytes was performed using the same anti-glial fibrillary acidic protein antibody described above (GFAP; Abcam, ab53554; 1:250 dilution). Secondary antibody incubation labeling GFAP positive cells was performed for 1 hour at room temperature using Alexa Fluor 488-conjugated Donkey anti-Goat (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by heat-based antigen retrieval in citrate buffer (pH 6) and primary antibody incubation (anti-ATP6V1B1, Abcam ab192612, 1:500) diluted in PBS/1%BSA ...
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bioRxiv - Neuroscience 2021Quote: ... brains were incubated in primary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Chicken anti-GFP (Abcam ab13970; 1:1000); anti-TH mouse monoclonal (SIGMA MAB318 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used and dilution ratios are: mouse monoclonal anti-Acetylated α-Tubulin (1:500, ab24610, clone 6-11B-1, abcam, Cambridge, MA), mouse monoclonal anti-Acetylated α-Tubulin (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were processed in a DAKO autostainer according to the manufacturer’s protocol using the same antibodies or anti-MITF ab12039 (Abcam, Cambridge, UK, 1:1,000). For immunofluorescence staining of KDM5B ...
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bioRxiv - Neuroscience 2022Quote: ... The CTB signal was detected by combining the RNAScope Multiplex Fluorescent v2 assay with immunofluorescence using a rabbit anti-CTB primary antibody (Abcam, ab34992, 1:200). The integrated RNA protein co-detection workflow was followed using the RNA-Protein Co-detection Ancillary Kit (Advanced Cell Diagnostics ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBST for 1 h at room temperature and then immunoblotted overnight at 4 °C with corresponding primary antibodies: anti-FTO (Abcam, ab124892, 1/1000) or anti-β-Actin (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunostaining was performed with the following antibodies at 1:50–500 dilutions in blocking solution for 1–2 hours at room temperature: anti-DDDD-K (Abcam, Cambridge, UK, ab1162), anti-GFP (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane and probed with well-characterized monoclonal antibody of anti-Fibrillin 1 (Abcam ab124334, Cambridge, UK).
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 h at room temperature with anti-rabbit Alexa Fluor A555 antibody (ab150078, 1:1000, Abcam, Cambridge, United Kingdom). Slides were washed with PBS and coverslipped with DAPI Fluoromount-G (0100-20 ...
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bioRxiv - Immunology 2019Quote: ... The tissue was embedded in paraffin and sections were stained with an antibody against RELM-α (polyclonal rabbit anti-mouse, Abcam, Cambridge, UK). Nuclei were stained with haematoxylin (Merck) ...
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bioRxiv - Developmental Biology 2020Quote: ... cover slips were incubated in primary antibody at 4°C overnight followed by rinses in PBS and one hour incubation with goat anti-mouse IgG (Alexa Fluor 647-conjugated) secondary antibody (Abcam, Cambridge, MA, ab150115) and/or goat anti-rabbit IgG (Alexa Fluor 594-conjugated ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 9 was performed using citrate buffer and sections were incubated with the primary antibody rabbit anti‐mouse CD31 (1:50) (ab28364, Abcam, Cambridge, MA, USA) at 4°C overnight ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 6 with citrate buffer and the primary antibody rabbit anti-mouse CD11b (1:200) (ab133357, Abcam, Cambridge, MA, USA) and antigen retrieval with 1:20 proteinase K/TE buffer and rat anti-mouse F4/80 (1:100 ...
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bioRxiv - Plant Biology 2019Quote: ... For the localization of total fraction of RNAPII we used mouse 8WG16 antibody (anti-RNA polymerase II CTD repeat YSPTSPS, Abcam, diluted 1:200), according to protocols provided by Kołowerzo-Lubnau et al64 ...
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bioRxiv - Physiology 2019Quote: Cyst sections were blocked and permeabilized with 3% normal donkey serum in PBS with 0.3% Triton X-100 for 2 h and incubated overnight with rabbit anti-beta-III tubulin antibody (ab18207, 1:2000, Abcam, Cambridge, United Kingdom) in 3% normal donkey serum in PBS with 0.3% Triton X-100 at 4°C ...
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bioRxiv - Physiology 2019Quote: ... for 1 h at room temperature and next incubated with primary antibodies (p62, CoxIV: Cell Signaling,3E11, Tomm20:Abcam, UK, ab56783 (anti-mouse) or ab186734 (anti-rabbit) ...
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bioRxiv - Developmental Biology 2020Quote: ... After the incubation with the primary antibodies the embryos and pupal dissections were washed three times for 30 minutes in PBT and incubated with the respective secondary antibodies: Alexa Fluor 488 conjugated Donkey Anti-Goat (abcam Cat. No: ab150129), Alexa Fluor 488 conjugated Goat Anti-Rabbit (ThermoFisher Scientific Cat ...
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bioRxiv - Genetics 2021Quote: ... Samples were then divided into two 250 μl aliquots and stained at room temperature for 1 hr with anti-RPA1 antibodies (rabbit, Abcam ab79398; 1:100) to detect endogenous RPA1 or anti-V5 tag antibodies (mouse ...
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bioRxiv - Physiology 2020Quote: ... diluted in PBTD was added for 2 hours followed by an anti-elastin rabbit polyclonal antibody diluted in PBTD 1:100 (Abcam, Cambridge, UK; ab21610). The samples were incubated with the primary antibody for 18 hours at 4°C followed by 30 minutes washing in PBTD ...
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bioRxiv - Neuroscience 2021Quote: ... the immunofluorescence staining as carried out as described above but adding also a primary antibody for TNFR1 (Rabbit anti-TNFR1, 1:500, product #ab19139, Abcam, Cambridge, MA, USA) and a secondary antibody (Alexa Fluor 594 donkey anti-rabbit antibody ...
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bioRxiv - Synthetic Biology 2021Quote: ... Periplasmic and cytoplasmic fractions were then run on SDS-PAGE gels and Western blotting was performed according to standard protocols using the following antibodies: anti-FLAG-HRP (Abcam ab49763; 1:2,500), anti-PhoA (Millipore MAB1012 ...
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bioRxiv - Biochemistry 2021Quote: ... then incubated in 5% milk in TBS-T containing 1:10000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: 5-FU and wdr12 samples were immunolabeled with anti-HuC/D antibodies (not shown) (ab210554, abcam, used at a dilution of 1:600). Anti-GFP (GFP-1020 ...
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bioRxiv - Bioengineering 2021Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Genomics 2021Quote: ... Secondary antibodies used in this study were as follows: goat anti-rabbit IgG Alexa Fluor 555 (1:500 dilution; abcam; Cat. No. ab150078), goat anti-mouse IgG Alexa Fluor 488 (1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 minutes each and incubated in the dark with their corresponding secondary antibody: Goat anti-rabbit Alexa 647 (1:1000 dilution, Abcam, Waltham, MA, USA) and Goat anti-mouse Alexa 594 (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated with primary antibodies (ON; 4°C) that were appropriately diluted in blocking solution: chicken anti-GFP (Abcam; ab13970; 1:500), rabbit anti-GFP (Santa Cruz Biotechnology ...