Labshake search
Citations for abcam :
801 - 850 of 2131 citations for Multiplex Assay kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 300μg of protein was used for immunoprecipitation assay using immunoprecipitation kit containing Dynabeads-coupled protein G (magnetic beads) and human anti-MMP9 (1:700) (Abcam, ab38898). Briefly the protein was mixed with anti-MMP9 antibody and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: LAL activity was determined in control and VPS35-deficient Hepa1-6 cells using the LysoLive™ Lysosomal Acid Lipase Assay Kit (ab253380, Abcam) according to the manufacturer’s instructions for adherent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The measurement of Cathepsin L activity was performed following the instructions and the protocol of Magic Red Cathepsin Assays Kit (Abcam, ab270774). Photos were taken using a Floid Cell Imaging Station (Thermo Fisher Scientist) ...
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bioRxiv - Immunology 2023Quote: ... Thymocytes were isolated from untreated and TBI-treated mice and intracellular pyruvate and lactate levels were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega ...
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bioRxiv - Genetics 2022Quote: ATPase activity of cohesin was measured using EnzChek Phosphate Assay Kit with purified recombinant proteins depleted of free phosphate using Inorganic Phosphate Binding Resin (Abcam: ab270547). Reactions were assembled with10nM cohesin ...
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bioRxiv - Neuroscience 2023Quote: Measurement of the oxygen consumption rate (OCR) in hiPSC-derived neurons was performed with the fluorescence-based Extracellular O2 Consumption Assay Kit (Abcam, ab197243) as described previously (Castelo Rueda et al. ...
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bioRxiv - Microbiology 2023Quote: ... Infected cell lines were incubated at 37°C in a carbogen environment for two hours prior to conducting cytotoxicity assays using a lactate dehydrogenase (LDH) kit (Abcam, UK) as previously described(48) ...
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bioRxiv - Biochemistry 2023Quote: ... The lipid fraction from 1 million cells per sample was extracted after which total cholesterol was assayed using the Cholesterol/Cholesteryl Ester Quantitation Assay kit (Abcam, ab65359) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: For measuring cellular iron in Fe2+ and Fe3+ states as well as total iron content we have a used a colorimetric Iron Assay Kit system (Abcam, ab83366) in stepwise manner as described in the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: The activity of Cathepsin L from isolated cardiomyocytes from 6-8 mice (plated as described) was assessed using the Cathepsin L Activity Assay kit from Abcam (ab65306). 50μg of protein from isolated cardiomyocytes were used to assess the degradation of the FR-AFC substrate at 37°C degrees for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transduced with the Sh2 or Scr control constructs on 4 DIV and cellular ATP measurements were performed on 18 DIV using a Luminescent ATP Detection Assay Kit (Abcam, ab113849). To block luminescence signal contamination from adjacent wells ...
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bioRxiv - Biochemistry 2024Quote: Glycine concentration in homogenates of HepG2 cells or fibroblasts (control and NKH) was measured by a fluorometric Glycine Assay Kit (Abcam, ab211100).
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... For CCK-8 assay (Abcam, ab228554), 3x 103 cells were seeded in 96-well plates and cultured for 6 days in complete medium (DMEM plus 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... and tetramethylrhodamin-ethylester (TMRE) assay (Abcam) was performed on DIV14 according to manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... and Cholesterol Assay (Abcam, Paris, France) kits ...
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bioRxiv - Cancer Biology 2024Quote: The alkaline comet assay (Abcam #ab238544) for assessing DNA damage in MCF-10A cells was performed according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Colorimetric creatine kinase activity assay (Abcam) was performed after each Seahorse assay followed by BCA protein assay (Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... XTT viability assays (Abcam, Cambridge UK) were used according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: An ALP activity assay (Abcam, England) was used to determine cell-dependent osteogenic activity (n=6) ...
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bioRxiv - Physiology 2024Quote: A membrane fluidity assay (abcam, ab189819) was performed while following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Phenol-red free media surrounding scaffolds for 14 days of hMSC cell culture on scaffolds was pooled and 80 μL of this sample was used with an Alkaline Phosphatase Assay Kit (Abcam, United Kingdom) along with phenol-red free media as a background control (n=6) ...
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bioRxiv - Neuroscience 2020Quote: The glutamate concentration in the conditioned medium of primary microglial cultures was assayed with the glutamate colorimetric assay kit from Abcam (no. 83389), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Indirect assessment of glutamate uptake by resting iPSC-astrocyte lines was done using a colorimetric glutamate assay kit following the manufacturer’s recommendations (Abcam; Cat. no.: ab83389). Briefly ...
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bioRxiv - Microbiology 2022Quote: Enzyme activity in vaginal swab supernatants and cell pellets was quantified using the α-Glucosidase Activity Assay Kit (Abcam, Waltham, MA, USA), Enzchek Ultra Amylase Kit (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: GAPDH activity was measured in the presence and absence of GAs using Glyceraldehyde 3 Phosphate Dehydrogenase Activity Colorimetric Assay Kit (ab204732, Abcam, Cambridge, MA) as per the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentrations of NAD(H) (total NAD+/NADH) and NADH only were measured by the NAD+/NADH assay kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Absolute quantification of sialic acid in different glycoconjugate fractions was performed using the Sialic Acid (NANA) Assay Kit (Ab83375, Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Total protein levels were measured by BCA assay (Pierce) and a 1:00 dilution was used for quantifying α-synuclein using the mouse α-synuclein ELISA kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... serotonin and brain derived neurotrophic factor using commercially available Enzyme linked immunosorbent assay kits according to the instructions of the manufacturer (ABCAM, Cambridge UK)
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bioRxiv - Bioengineering 2023Quote: ... was dissected from the constructs at the end of maturation time (day 15) and tested via a colorimetric CK Activity Assay Kit (Ab155901, Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... None of the cultures or treatments showed signs of cell cytotoxicity using the using the LDH-Cytotoxicity Assay Kit II (Abcam, cat. #ab65393) to analyze 50 ul fresh culture media (not shown) ...
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bioRxiv - Neuroscience 2024Quote: Choline and acetylcholine were quantified in rat cortical tissue from all experimental groups using a choline/acetylcholine assay kit (ab65345, Abcam, Cambrige, UK). 10 mg of cortical fresh tissue were harvested ...
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bioRxiv - Physiology 2024Quote: Mitochondrial membrane potential was measured in γY4KD cells with Pparγ silenced with siRNA and their scrambled control (Scrl) using Mitochondrial Membrane Potential Assay kit (Abcam, Cat# ab113852). Cells were plated on collagen coated 24-well plate at density of 50,000 cells/well (5 well/per group) ...
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bioRxiv - Cancer Biology 2023Quote: ... end-stage bulk tumor and 4- to 15-weeks control HDTVi liver protein lysates (50 μg) were analyzed using Fluorometric Caspase-1 Assay Kit (Abcam, cat.no. ab273268) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: D2HG levels were measured in adherent cultures of 1 x 107 chondrosarcoma cells treated with increasing concentrations of Enasidenib for 48 hours using the D2HG colorimetric Assay Kit (Abcam, Cambridge, UK) following the manufactureŕs recommendations.
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bioRxiv - Cell Biology 2024Quote: ... MockIP and WCL were aliquoted into wells in a 96-well plate and assayed using a fluorometric Cathepsin D activity assay kit (Abcam. Cat# Ab65302) that is based on quantifying the hydrolysis of the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2 ...
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bioRxiv - Immunology 2021Quote: ... Free fatty acid concentration in plasma (diluted 1:25) of clamped mice was determined using a quantitative Free Fatty Acid Assay Kit (Abcam, Cambridge, Great Britain). Absorbance was measured with the Filter Max F5 device (Molecular Devices ...
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were rinsed with phosphate-buffered saline (PBS) and then stained in the culture well using the Adipogenesis Assay Kit (cell-based, Abcam Catalog No. ab133102). The dye was eluted using the kit’s elution buffer and quantified using a microplate reader at 490 nm wavelength ...
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bioRxiv - Immunology 2022Quote: ... media was collected and LDH activity measurement was carried out in 96-well plate (Costar, cat no. 3370) according to LDH assay kit (Abcam, cat. no ab102526). Absorbance measurement at 450 and 570 nm was carried out using microplate reader (BioTek ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were collected after 24 h and the caspase activity was determined using the Caspase-3 Assay Kit (Colorimetric) (Abcam ab39401, Cambridge, UK).
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bioRxiv - Molecular Biology 2021Quote: ... The serum is at the top layer and was collected for subsequent colorimetric analysis of the total cholesterol using the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, Cambridge, MA, USA) following manufactures protocol.
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bioRxiv - Physiology 2023Quote: ... Unless specified below, all reagents were from MilliporeSigma (St. Louis, MO, USA). LDH assay kit (catalog no. ab65393) was from Abcam (Cambridge, MA, USA). P ...
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bioRxiv - Cell Biology 2023Quote: The chymotrypsin-like activity of the 20S proteasome was assessed using a commercially available fluorometric proteasome activity assay kit (Abcam, catalog number: ab107921) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: Chromatin accessibility in isolated cardiac fibroblasts or whole heart from C57BL/6J mice was measured using the Chromatin Accessibility Assay Kit (Abcam, cat. no. ab185901) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The amount of collagen in liver tissues was determined by measuring the content of hydroxyproline using a Hydroxyproline assay kit (Abcam, Cat#MAK008-1KT) as described by the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: LB samples that were autoclaved or not autoclaved were subjected to quantification of NADH/NAD+ using an NAD+/NADH Colorimetric Assay Kit (ab65348, Abcam, Cambridge, United Kingdom). Autoclaved samples were measured before and after either chronoamperometry or cycle voltammetry treatment ...