Labshake search
Citations for abcam :
751 - 800 of 2131 citations for Multiplex Assay kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... supernatants from the cocultures were tested in a hemoglobin release assay at a dilution of 1:1600 (Human Hemoglobin ELISA kit, Abcam) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... the quantification of changes in mitochondrial membrane potential was determined following the protocol of the TMRE-Mitochondrial Membrane Potential Assay Kit (Abcam). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... cell viability was evaluated at the end of incubations by a dehydrogenase activity assay (Cell Counting Kit 8, Abcam #ab228554), and expressed relative to the solvent control.
-
bioRxiv - Microbiology 2023Quote: ... A bacterial culture containing about 109 exponentially growing Leptospira was used to determine the catalase activity using the Catalase Activity Assay Kit (Abcam), according to manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... Total nitrite/nitrate was measured as a proxy for nitric oxide concentration using the Nitric Oxide Assay Kit (Abcam, ab65327), with 5 hours of incubation with the enzyme cofactor to permit more than 99% conversion of nitric oxide to nitrite.
-
bioRxiv - Cell Biology 2022Quote: Cell culture supernatant of differentiated Hoxb8 macrophages was collected and the amount of soluble Galectin-3 was quantitatively assessed using the Galectin-3 Mouse SimpleStep enzyme-linked immuno-sorbent assay (ELISA) kit (Abcam) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: The Dihydroethidium (DHE) assay kit for the measurement of intracellular superoxide and hydrogen peroxide was purchased from Abcam (Cat#ab236206). The MitoSOXTM for the measurement of mitochondria superoxide was purchased from ThermoFisher Scientific (Cat#M36008) ...
-
bioRxiv - Cancer Biology 2023Quote: Changes in mitochondrial membrane potential produced by I3C and BNF in the five cancer cell lines was determined with the tetramethylrhodamine ethyl ester (TMRE) Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... Reactions were transferred to a 96-well plate and the amount of inorganic phosphate released from the hydrolysis of NTP was determined using Phosphate Assay Kit PiColorLock (Abcam) according to protocol by the manufacturer ...
-
bioRxiv - Cancer Biology 2023Quote: We measured mitochondrial membrane potential (MtMP) using the JC-1(tetraethylbenzimidazolylcarbocyanine iodide) mitochondrial membrane potential assay kit (Abcam; Cat #113850) following the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: The acetyl-CoA content of the cell culture medium was determined with the Acetyl-CoA Assay Kit (Reference: ab87546, Abcam) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... and the cells were lysed in 80 μL of ice-cold buffer from Glutathione Assay Kit (catalog no: ab239709, Abcam). The lysed cells were kept on ice for 10 minutes followed by mixing thoroughly with 20 μL of 5% SSA (sulfosalicylic acid ...
-
bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
-
bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
-
bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
-
bioRxiv - Physiology 2024Quote: We utilized two commercially available enzyme-linked immunosorbent assay (ELISA) kits for the quantitative analysis of IL-6 (Abcam, ab100713) and IL-8 (Abcam ...
-
bioRxiv - Immunology 2024Quote: ... mouse PGE2 ELISA Kit (ab287802and Cytokine & Chemokine 36-Plex Mouse ProcartaPlexTM Panel 1A (EPX360-26092-901) for Luminex assay were obtained from Abcam and Thermofisher respectively ...
-
bioRxiv - Neuroscience 2024Quote: ... GDH activities in primary astrocytes were determined with glutamate dehydrogenase activity assay kit following with the manufactureŕs instructions (Abcam, ab102527).
-
bioRxiv - Microbiology 2024Quote: The SOD activity of the gastrocnemius muscle tissues from the mice was assessed using the colorimetric assay kit (Abcam, ab65354) as per the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... then resuspended at 1×105 cells/mL in ice-cold PBS and analysed using the comet assay kit (Abcam, #ab238544) according to manufacturer’s instructions with gel electrophoresis run for 20 minutes at 3 volts/cm.
-
bioRxiv - Cancer Biology 2024Quote: Matrix metalloproteinase (MMP) activities (as a general enzyme class) were assayed using abcam MMP Activity Assay Kits (Cat # ab112146, Fluorescent [green]; abcam, 152 Grove Street ...
-
bioRxiv - Cell Biology 2024Quote: Enzymatic activity of secreted LOX was measured in 10 μl cell culture supernatants using the fluorometric Lysyl Oxidase Activity Assay Kit (Abcam). LOX activity kinetics was evaluated by measuring fluorescence (Ex/Em = 535/587 nm ...
-
bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
-
bioRxiv - Neuroscience 2023Quote: ... the BrdU assay (ab126556, Abcam) was performed for three replicates at D0 ...
-
bioRxiv - Physiology 2023Quote: AGE assays (Abcam, Cambridge, MA) were performed on each patient’s tissue samples ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were measured using the BIO-RAD Protein Assay or Bradford assay (Abcam 119216) and adjusted with apoplast extraction buffer to a final concentration between 0.5-1.5 mg/mL ...
-
bioRxiv - Cell Biology 2019Quote: The TC and free cholesterol content of hLECs were measured colorimetrically using a Cholesterol assay kit according to the manufacturer’s protocol (#ab65359; Abcam, Cambridge, UK). Harvest the amount of hLECs necessary for each assay (initial recommend = 1 x 107 cells) ...
-
bioRxiv - Cell Biology 2022Quote: ... NAD+/NADH ratio and the concentration of total NAD+ and NADH was determined by NAD/NADH fluorometric assay kit from Abcam (ab176723) following manufacture’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... The general activity of MMPs in iPSC-astrocyte supernatants was determined using the MMP Activity Assay Kit (Abcam, Cat. no.: ab112147) following the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2021Quote: ... Twenty microliters of reagent was added directly to each well plate following the instructions of the MTS Cell Proliferation Assay Kit (colorimetric ab197010, abcam®). The culture plate was incubated for 3 h at 37°C in a humidified atmosphere containing 5% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... Serum concentrations of immunoglobulin A (IgA) and immuno-globulin M (IgM) were measured using enzyme-linked immunosorbent assays kits (ELISA; Abcam, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: The activity of mitochondria Complex I was measured in peeled murine esophageal epithelium using Complex I Enzyme Activity Assay kit (Abcam, ab109721) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with filipin III (1:100) in PBS for 2 h as described in the manufacturer’s protocol of the cholesterol assay kit (Abcam Biochemicals, ab133116). Filipin III was also added in all following incubation steps with the primary and secondary antibody to enable proper staining ...
-
bioRxiv - Cell Biology 2021Quote: Cathepsin B activity was assessed in mouse primary neuronal samples and mouse brain hippocampus using Fluorometric Cathepsin B activity assay kit (abcam, 65300) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... Alanine liberated was measured as increasing fluorescence signal (Resorufin, Ex/Em: 535/587 nm) recorded at 25 °C using an L-alanine assay kit (Abcam, ab83394) at 25 °C according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... IL-6 levels in TCM of met-low and met-high melanoma and breast carcinoma tumors were quantified using a specific enzyme-linked immunosorbent assay (ELISA) kit (ab46100, Abcam, US). The ELISA experiments were performed with 5-8 mice per group ...
-
bioRxiv - Cancer Biology 2019Quote: A luminescent ATP detection assay kit was used to determine the total levels of cellular ATP as per manufacturer’s instructions (abcam, Cat #ab113849). Briefly ...
-
bioRxiv - Cell Biology 2021Quote: The relative contents of endogenous sphingomyelins in WT and SIRT1 KO mESCs were analyzed using a commercially available Sphingomyelin Assay Kit (Abcam ab133118) per manufacturer’s instruction.
-
bioRxiv - Neuroscience 2022Quote: The quantitative detection of serum lipopolysaccharide binding protein (LBP) was performed by Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (Abcam, ab213876) according to the manufacturer protocol ...
-
bioRxiv - Neuroscience 2023Quote: Conditioned media from the somatic side of the same MFCs used for the ELISA was analysed with the Cytotoxicity LDH assay kit (Abcam #ab65393) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... and nuclear DNA-encoded succinate dehydrogenase A (SDH-A) using the MitoBiogenesis In-Cell Enzyme-linked immunosorbent assay (ELISA) Kit (Colorimetric, Abcam, ab110217) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: RalGDS RBD Agarose beads were used to selectively pull-down the active form of Rap1 (Rap-GTP) from isolated cardiac mitochondria (Rap1 Activation Assay Kit, Abcam, ab212011). Samples of mitochondria ...
-
bioRxiv - Cancer Biology 2023Quote: ... The pellet was centrifuged once again and re-suspended in nuclear extraction buffer from the kit to generate the nuclear fraction and samples used for the NF-κB p65 transcription ELISA based assay (Abcam, ab133112) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2024Quote: The mitochondrial membrane potential (ΔΨm) was measured with the JC-1 mitochondrial membrane potential assay kit (ab113580, Abcam, Cambridge, United Kingdom) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... ROS indicator dye was prepared according to the manufacturer’s protocol using DCFDA/H2DCFDA - Cellular ROS Assay Kit (Abcam, ab113851, Cambridge, UK), and was added to each well ...
-
bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: For measuring cellular iron in Fe2+ and Fe3+ states as well as total iron content we have a used a colorimetric Iron Assay Kit system (Abcam, ab83366) in stepwise manner as described in the manufacture’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: Detection of cytoplasmatic and mitochondrial ROS levels was performed by means of a commercial DCFDA / H2DCFDA - Cellular ROS Assay Kit (abcam, ab113851) and MitoSOX™ Red Mitochondrial Superoxide Indicator (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... The preparation of samples and lactate level measurement procedure were followed as specified in the Lactate Assay Kit (Colorimetric) (ABCAM - ab65331) according to the manufacturer’s instructions ...