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Citations for abcam :
701 - 750 of 810 citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated for 48 hours at 4 °C with primary antibodies diluted in 5% NGS in PBS-T as follows: rabbit anti-cFos (1:1000, Abcam, #190289) and chicken anti-GFP (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were blocked with 5% BSA in PBS for 30 min at room temperature and incubated with anti-Rad51(abcam ab133534) primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008 ...
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bioRxiv - Developmental Biology 2022Quote: ... Membrane was blocked for one hour in 5% of milk or TBS-T and incubated overnight at 4°C with anti-vinculin (Abcam 73412), anti-CREB (Santa Cruz SC-186) ...
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bioRxiv - Neuroscience 2022Quote: ... and 20 μM ThT in the presence or absence of purified recombinant NRN1 (5 μg/mL or 263 nM; Abcam, ab69755) protein ...
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bioRxiv - Neuroscience 2022Quote: ... The rats received bilateral lesions in the LHb with ibotenic acid (5 µg/µl in 0.01 M PBS, Abcam, Cambridge, UK). The coordinates for the LHb (AP −3.3 mm ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C in primary antibody diluted in blocking solution with 5% normal goat serum (Abcam, ab7481). Antibodies were directed against GFP (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... washed further 5 times with PBS pH 7.4 and incubated for one hour with Goat anti-monkey (Rhesus macaque) (Abcam cat # a112767), diluted 1:2000 in PBS pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... paraffin-embedded and 5 µm-thick sections were stained with hematoxylin and eosin (H&E) or picrosirius red solution (Abcam #ab150681) as in [18] ...
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bioRxiv - Genomics 2023Quote: ... in PBS-T for 1 hour and incubated overnight in 5% goat serum in PBS-T with primary antibody at 4°C (SON (1:300, abcam ab121759) or SRRM2 (1:300 ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies were diluted in 5% milk TBST and incubated with blots overnight at 4 °C (FGF1 – Abcam, ab207321; β-ACTIN – Abcam, ab8226 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS for 5 minutes and incubated with AlexaFluor® 647 conjugated secondary antibodies (Ab150079, Ab150115; Abcam) for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... pH 7.5) three times, incubated in blocking solution (1% [w/v] bovine serum albumin, 1% [w/v] normal goat serum (Abcam ab7481) in TBS with 0.2% [v/v] Tween-20 [TBST]) ...
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bioRxiv - Physiology 2023Quote: ... incubated with blocking buffer (PBS + 5%FCS +0,1%Tween) for 1 hour and stained with anti-Iba1 antibody (diluted 1:300, Abcam; Cat#ab178846) and /or anti-TOM20 antibody (diluted 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... For the intracellular S.Tm-LPS staining using α-S.Tm LPS (O-antigen group B factor 4-5, Difco) and α-rabbit-AlexaFluor647 (Abcam Biochemicals), cells were incubated with IC fixation buffer (eBioscience ...
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bioRxiv - Physiology 2023Quote: ... Frozen brains were sliced into 7um and incubated in 0.1%PBST + 5%BSA with anti-Iba1 antibody (diluted 1:300, Abcam; Cat#ab178846) and/or anti-GFP antibody (diluted 1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were stained overnight at 4°C with the following primary antibodies: anti-ORC1 (1:1000 TBST/5% milk; Abcam, ab85830). Immunoblots were then incubated in secondary goat anti-rabbit (1:1000 TBST/5% milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were then washed 5x 5 minutes each and incubated with Alexa594-conjugated Goat α-Mouse IgG H&L (Abcam, #ab150116) diluted 1:500 in wash buffer for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and next incubated for 1 hour in a solution of PB with 5% normal goat serum (Abcam Cat# ab7481, RRID: AB_2716553). Subsequently ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of total fragmented chromatin was saved as an input control and the remaining chromatin was split into two tubes (representing 5×107 cells each) for antibody pulldown and incubated overnight at 4°C with 15 ug of NRF1 (Abcam #ab55744), or MYC (Abcam #ab56 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then treated with 5% BSA in PBS for 90 minutes at 25°C followed by treatment with anti-Listeria antibody (Abcam: ab35132) at 4°C overnight ...
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bioRxiv - Genomics 2023Quote: ... 0.1% Tween 20) and incubated over night with the desired antibody diluted in 5% milk / TBST [1:1000 H3 (abcam ab1791); 1:1000 Val1744 cleaved NICD1 (Cell Signaling Technology 4147)] ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed 5 times with PBS and incubated in PBS with 10% FBS and 1:100 rabbit anti-synaptopodin (Abcam, ab224491), 1:1000 rabbit anti-N-WASP (Santa Cruz Biotechnology Inc ...
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bioRxiv - Physiology 2024Quote: ... antibody kindly gifted by Jan Nedergaard and Barbara Cannon) and anti-αTubulin to control for equal loading (1:1000 solution in 5% milk in TBS-T, Abcam 2144). The following day ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were re-stained with rabbit monoclonal anti-SMAD1/5 (SMAD1: 1:500, ab33902; SMAD5: 1:1,000, ab40771; both Abcam, Berlin, Germany) or rabbit monoclonal anti-SMAD2/3 (1:250 ...
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bioRxiv - Immunology 2023Quote: ... 5% non-fat skim milk) and subsequently incubated with a rabbit anti-HIV-1 p55+p24+p17 antibody (Abcam, 1:2000) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed in PBS three times (5 min each time) and incubated with a donkey-anti-rabbit Alexa488 secondary antibody (1:1000, Abcam, USA) in PBST for 2 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The tissues were washed three times with PBS following fixation and blocked with blocking buffer: 5% normal goat serum (NGS, Abcam, AB7681) in PBS with 0.1% Triton-X (PBST) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections measuring 5 μm were then cut using the Leica RM2165 and subsequently stained with Movat’s Pentachrome staining kit (Abcam Cat# ab245884). For H&E staining analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were incubated overnight at 4°C with primary antibodies diluted in 5% BSA: rabbit α-SYCP1 (Abcam, ab15090; 1:200) and mouse α- γH2AX (Millipore Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... then the brain slides incubated in a secondary antibody for 90 min protected from light at room temperature (0.1% TritonX-100, 5% normal goat serum, PBS solution with 1:500 goat anti-rabbit Alexa 488; Abcam, Cambridge, MA). Slides were washed again as described above ...
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bioRxiv - Developmental Biology 2021Quote: ... The mouse anti-DP Yun (#6, 1:5 (Du et al., 1996) and the mouse beta actin (1:1000, Abcam, Cat#ab8224) antibody was used as loading control in western blot assays.
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bioRxiv - Microbiology 2021Quote: ... Fixed worms were washed three times in PBS for 5 mins and transferred to new 12-well plates with 1ml per well of 1x Phalloidin-iFluor 647 Reagent (Abcam cat# ab176759) in PBS pH=7.4 for 90 mins at room temperature with moderate shaking ...
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bioRxiv - Neuroscience 2020Quote: ... The fixed cells were blocked with 5% normal goat serum for 4 hours followed by incubation with anti-Iba-1 antibody (1:300, Abcam, Cambridge, UK) for 12 hours at 4°C to label microglial cells ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C for 5-7 days in the primary antibodies containing: chicken anti-glial fibrillary acidic protein (GFAP) (targeting astrocytes, 1:200, Abcam #ab4674, USA) and/or and NeuN (targeting nuclei of neurons ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked in 5% milk for 1 hr at room temperature and incubated with primary DAZL antibody (ab215718, Abcam, 1:1000) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Pathology 2021Quote: ... The samples were then washed for 5 minutes three times with TBT and incubated with the secondary antibody (Thermofisher A-21121 and Abcam® ab97239) for an hour at a dilution of 1:200 ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was adjusted to 400 µg and incubated O/N at 4℃ with 5 µg of IP-antibody (Table S1) or rabbit IgG control (Abcam, Cambridge, UK) in a final volume of 500 µl of NT2 buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: Sections were briefly dried then washed 3x in 0.1% Triton X-100 in phosphate buffered saline (PBST) before they were blocked with 5% neutral goat serum (Abcam or Vector Labs). Sections were then incubated for double-labeling experiments with primary antibody (AB ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were tehen incubated with a primary antibody solution containing BrdU antibody diluted 1:400 or 1:1000 in PBS containing 5% goat serum (Abcam, catalog # ab7481) and 0.1% Triton-X for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was first blocked in PBS 1X 0,02% Tween-20 containing 5% Milk and incubated overnight with the dsDNA-specific antibody (ab27156 Abcam, dilution 1/1000). To quantify G4 formation ...
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bioRxiv - Neuroscience 2019Quote: ... GluN receptors were blocked by adding 4 μl of 25 mM d,l-2-amino-5-phosphonovaleric acid (APV; Abcam Cat# ab144498) to the 1 ml of aCSF in the interface recording chamber ...
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bioRxiv - Immunology 2021Quote: ... iPS cells were treated for 1 h with HDR enhancer (5 μM; IDT, Coralville, USA) and 10 μM Y-27632 (Abcam, Cambridge, UK). Electroporated cells were seeded on Laminin 521 (Biolamina ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was then treated with a blocking solution for half an hour (5% skim milk in 1% TBST) and incubated with the primary antibody (ab138501, Abcam, Cambridge, UK) at a ratio of 1:5,000 overnight at 4°C under constant motion ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed three times with TBST for 5-min and then incubated for 1-hr with horseradish peroxidase-conjugated goat anti-rabbit IgG (ab6721, Abcam, Cambridge, MA). Membranes were washed three more times with TBST for 5-min.