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Citations for abcam :
551 - 600 of 810 citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with 5% milk in 0.1M TBST and probed with primary antibody: GAPDH (Mouse, Abcam G8795,1:1000) was used as an internal control for the concentration of protein loaded ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 5% donkey serum for 2 h with primary antibodies against cardiac troponin I (ab47003, Abcam: 1:800) and for 1 h with secondary antibodies conjugated with Cy5 (711-175-152 ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBST pH 7.6 for 5 min and incubated overnight at 4 °C in primary antibody (Mouse-anti-myc [Abcam #ab32 ...
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in 0.3% PBT and incubated with secondary antibodies and Hoechst (Abcam) in 3% NDS in 0.3% PBT for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with PBST (Triton 0.3 %) solution containing 5 % donkey normal serum and one or more antibodies (goat Anti GFP, ab6673, Abcam, diluted 1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20) and incubated overnight with primary antibodies diluted in 5% milk in TBST (anti-ELK1 (Abcam ab32106) 1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... All chambers were again washed twice with PBS before a blocking solution consisting of 5 % goat serum (Abcam, ab7481) diluted in PBS was added to the cultures ...
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bioRxiv - Biophysics 2024Quote: ... The membrane was blocked in 5% milk in TBS-T buffer and probed with anti-CPEB4 antibody (Abcam ab224162).
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bioRxiv - Cancer Biology 2021Quote: ... cells were blocked in blocking buffer (5% bovine serum albumin in PBS) for 1 hour and incubated with the primary antibody RAD51 (ab133534, Abcam) or phosphor-H2AX (#05-636 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μl diluted cross-linked chromatin was incubated overnight with 5 μg mouse monoclonal anti-TEAD4 antibody (Abcam, Cat. #ab58310), 5 μg mouse monoclonal anti-YAP antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were blocked in 20% NGS for 30 min (Ki67) or 5% NGS for 60 min (CC3) before incubation in primary antibody overnight (anti-Ki67, Abcam; anti-cleaved Caspase 3 ...
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bioRxiv - Cell Biology 2020Quote: ... Fixed and permeabilized cells were then incubated with 1:400 dilution of Alexa Fluor 647 conjugated mouse anti-human cytokeratin 5 antibody (Abcam) for 24 hours at 4C in permeabilization buffer ...
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bioRxiv - Genetics 2021Quote: ... blocked with PBS/5% goat serum, and then incubated with primary antibodies: Mobp (1/1000, rabbit, Abgent) and Cnp (1/1000 mouse, Abcam) prior to exposure to fluorescent Alexa secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated for 10 min at 37 °C in the presence of 10 µM 2-amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile (UCPH-101; Abcam 120309, UK), a specific excitatory amino acid transporter 1 (EAAT1/Glast ...
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bioRxiv - Microbiology 2022Quote: ... and incubated overnight with primary antibodies diluted in 5% BSA: rabbit anti-PCBP1 (clone EPR11055, Abcam ab168378, diluted 1:10,000); rabbit anti-actin (A2066 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 8×5 min in PBS before being mounted onto glass slides and cover-slipped with fluoroshield mounting medium with DAPI (AbCam). Dual staining was performed with sequential primary-secondary incubations ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...
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bioRxiv - Cell Biology 2019Quote: ... and then diluted with pre-chilled lysis buffer at a 1:5 ratio and subjected to immunoprecipitation using anti-CI-30 (cat#: ab14711, Abcam).
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bioRxiv - Genomics 2019Quote: ... Immunoprecipitation was performed overnight at 4°C with rotation using 1-2 × 106 cell equivalents per immunoprecipitation using antibodies (5 µg) against H3K4me1 (Abcam), H3K27ac (Abcam) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000, Abcam, U.S.A), followed by incubation with horseradish peroxidase-linked secondary antibodies (1:5,000 ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... cells were incubated 5 min with blocking solution (1% FBS, 0.5% BSA) and 30 min with anti-VASH1 antibody (1/50; abcam ab176114) and Alexa 488 conjugated anti-mouse secondary antibody ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed by incubation of 2 ml diluted chromatin (equivalent to 5×106 input cells) with antibodies for H3K4me1 (3 µg ab195391, lot: GR304893-2; AbCam), H3K4me3 (1 µl 07-473 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were blocked in 5% foetal bovine serum (FBS) for 30 min and incubated overnight in rabbit anti-PSD-95 (1:100; Abcam, Cambridge ...
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A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studiesbioRxiv - Cancer Biology 2020Quote: Formalin fixed paraffin-embedded sections (5 μm) were stained as described previously34 using the following primary antibodies: rabbit anti-Ki67 (ab15580, Abcam), rabbit anti-OLIG2 (ab109186 ...
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bioRxiv - Neuroscience 2019Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes incubated with the primary antibodies against claudin-5 (1:1000, Zymed 1600-34) or GAPDH (1:400, ab181602, ABCAM)) and the appropriate secondary antibodies.
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bioRxiv - Microbiology 2021Quote: Clonal lines were screened for knockout by labeling with primary mouse monoclonal antibodies to CD59 (clone MEM-43/5; Abcam) or CD46 (clone C-10 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked with 5% BSA in TBST then probed with primary antibodies for HMGCR (Ms mAb, 1:000, abcam), MVD (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked with 5% non-fat skim milk and immunoblots were performed with indicated primary antibodies [anti-SUMO1 (Abcam), anti-Actin C3 (Abiocode) ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed and then incubated at RT for 1 h with horseradish-peroxidase (HRP)-conjugated goat anti-mouse (IgG) polyclonal antibody as the secondary antibody (1:5 000; Abcam). After three washes ...
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bioRxiv - Cell Biology 2020Quote: ... and heated to 95 °C for 5 min (no heating of the samples used for the total oxphos (abcam, ab110413) blots) ...
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bioRxiv - Neuroscience 2020Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in TBS + 2% BSA + 0.1% Triton X-100 at 5 µg/mL (rabbit anti-OCT4, Abcam #ab19857), 1:200 (mouse anti-NANOG ...
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bioRxiv - Cell Biology 2022Quote: ... paraffin slices were blocked for 1 hour at 37°C with 5% BSA before being treated overnight at 4°C with various primary antibodies as follows: anti-GFAP (Abcam), anti-CD68 (Abcam) ...
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bioRxiv - Physiology 2022Quote: ... blocked in 5% goat serum for 1 hour and stained using myelin protein zero (MPZ) (1 in 50 in 5% goat serum in PBS containing 1% triton) (EPR20383, Abcam) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed with 1xPBS 3x for 5 minutes before incubation in 50 µl spots of secondary antibodies (goat anti-mouse AF647: Abcam ab150115 ...
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bioRxiv - Neuroscience 2019Quote: ... injected animals followed a slightly different protocol where slices were blocked with 5% donkey serum instead of BSA and the secondary antibody was donkey anti-rabbit IgG FITC 1:500 (ab6798, Abcam). Colocalization was quantified manually from 40x images.
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bioRxiv - Neuroscience 2021Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were permeabilized with 0.1% Triton X 100 in 1X PBS for 5 min and blocked with Protein Block (Abcam, USA) for 20 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Samples were blocked with 5% normal goat serum for 1 hr and incubated in anti-TRPM4 (Abcam, catalog no. ab106200) or normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... 1×106 cells were blocked with DPBS with 5% of normal mouse serum (cat. n° ab7486, Abcam, Cambridge, MA, USA) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... 0.25 x 106 cells were seeded into 10 mL cell culture dishes then treated with 5 ng/mL TGFbeta (Abcam) on the next day ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBS-T with 5% skim milk powder at 4°C overnight and incubated in 1:8000 diluted anti-GFP antibody (Ab290; Abcam) in TBS-T with 5% skim milk powder for one hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Systems Biology 2021Quote: ... All primary and secondary antibodies were diluted 1:400 in PBS containing either 2.25% bovine serum albumin [Millipore Sigma #A2058] or 5% normal goat serum (Abcam #ab7481) for blocking and 0.15% Triton X-100 (Sigma Aldritch #T8787 ...
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bioRxiv - Immunology 2022Quote: ... were washed with lysis buffer (containing protein inhibitors) and incubated with 5 µg of 4C4 antibody or IgG1k as a control isotype (ab18443 Abcam) at 4°C overnight under rotation ...
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bioRxiv - Molecular Biology 2019Quote: ... Blots were blocked for 1 hour with 5% milk in PBS and probed with anti-H3 (Abcam, ab1791; 1:2,000), anti-H3K4me3 (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... Blots were blocked for 1 hour with 5% milk in PBS and probed with anti-H3 (Abcam, ab1791; 1:2000), anti-H3K4me3 (Abcam ...
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bioRxiv - Genomics 2019Quote: ... The cells were blocked with 5% BSA in PBST for 30 min at RT and incubated with primary antibody (monoclonal mouse anti human SC35, Abcam) at 1:250 dilution for 1 hr at 37°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 μL FLAG tag antibody (Anti-DDDDK-PE, Abcam ab72469, RRID: AB_1268475, or Anti-DDDDK-APC, Abcam ab72569, RRID: AB_1310127) was added at a concentration of 0.5 μg per sample and cells incubated at 4°C for 30 min ...