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Citations for abcam :
601 - 650 of 2638 citations for 3 Ethyl 3 methyloxolane 2 5 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and then mechanically dissociated by repeated pipetting and filtered through a 40-μm nylon mesh prior to adding 3 nM of DRAQ-7 (Abcam) for viability assessment for flow cytometry analysis and/or sorting ...
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bioRxiv - Developmental Biology 2024Quote: ... starting from 3-5x106 cell for TFs and 0.5-1x106 for H3K27ac and using the following antibodies: MEIS1( Abcam ab19867), GATA-6 XP Rabbit mAb (D61E4 ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 minutes in Working solution and 2 hours with a primary antibody: DDX4 (ab13840, Abcam) or γH2AX(Ser139 ...
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bioRxiv - Molecular Biology 2021Quote: A subgroup of mice received an intraperitoneal injection of 5-bromo-2’-deoxyuridine (ab142567, abcam) diluted in PBS at 25mg/ml ...
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Induction of telomerase in p21-positive cells counteracts capillaries rarefaction in aging mice lungbioRxiv - Molecular Biology 2022Quote: A subgroup of mice received an intraperitoneal injection of 5-bromo-2’-deoxyuridine (ab142567, abcam) diluted in PBS at 25mg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 mg/kg thymine analogue 5-bromo-2’-deoxyuridine (BrdU, Abcam, College Science Park, UK) was introduced into the peritoneum at 30 min before euthanasia ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat. Ab28364, Abcam, Cambridge, UK) diluted 1:50 in the same blocking buffer ...
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bioRxiv - Genetics 2021Quote: ... were incubated in blocking solution (0.5% bovine serum albumin in PBS) with 3 μg of antibody to histone-H3 K27ac (Abcam, cat. # ab4729) for 3 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Zoology 2020Quote: ... 0-3 days old) and older buds (stage 3, 1 month old) by two different means: anti Phospho-histone H3 (PHH3) immunolocalization (Abcam ab5176) antibody (1:200 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... In the next step the cells were washed with 3% BSA solution and incubated with 1x Phalloidin-iFluor594 (Abcam, stock 1000x) solution (in 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... The the membranes were incubated overnight at 4°C with rabbit anti-VEGFR-3 antibodies (Abcam, Cambridge, MA, USA; 1:50). Horseradish peroxidase (HRP)-conjugated secondary goat anti-rabbit antibodies (Sigma-Aldrich Co. ...
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bioRxiv - Cancer Biology 2020Quote: Beads that were incubated with FLI 7.3 mouse antibody were resuspended in 100 µL Dig-wash buffer and incubated with rabbit anti-mouse secondary antibody (Abcam, ab46540) at a dilution of 1:100 on a rotator for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... BLA slices were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS (3×10 min) and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113 ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Non-specific sites on the membrane were blocked using 3% BSA and further incubated with anti-SATB2 antibody (1:1000 abcam ab34735) in 0.3% BSA overnight at 4°C with constant rocking ...
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bioRxiv - Cell Biology 2020Quote: ... the ovarian samples were incubated overnight at 4°C with primary antibodies against caspase-3 (1:1000 dilution, Abcam, Cambridge, MA) and NLRP3 (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing goat anti-rabbit IgG H&L Alexa Fluor 488 (abcam; ab150077).
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bioRxiv - Cell Biology 2022Quote: ... log-phase liquid cultures were divided in two and incubated for different times in YES-Glucose medium treated with 10 µM 3-BrB-PP1 (Abcam, ab143756), or in medium lacking the analog kinase inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin embedded liver sections (3 μm) from human and mouse liver samples were stained for aSMA (1:100, Abcam, Cat#ab5694), KI67 (1:50 ...
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bioRxiv - Biophysics 2024Quote: ... The micro-tissues were then permeabilized in 0.5% Triton X-100 in PBS for 5 min and blocked in 3% bovine serum albumin (BSA) in PBS before they were incubated with primary antibodies (anti-sarcomeric alpha actinin (Abcam #9465) or desmin (Cell Signaling #5332) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 47 µl of Inside Perm Solution and stained with 3 µl of Anti-PBR antibody [EPR5384] (abcam, ab199836). After 10 minutes of incubation ...
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bioRxiv - Immunology 2023Quote: ... The sections were incubated overnight with anti-cell marker cluster of differentiation (CD) 3 antibody (1:200, Abcam, ab16669, Cambridge, UK) at 4°C in a humidified incubation chamber ...
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bioRxiv - Neuroscience 2022Quote: ... Slices containing VPMpc were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed on sectioned paraffin-embedded tumor constructs or fixed cells cultured for 3 to 7 days on X-tra® slides to analyze biomarker presence of SF-1 (ab240394, Abcam), Melan A (ab210546 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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bioRxiv - Biochemistry 2022Quote: Cells were incubated in complete media in the presence of 100 nM bafilomycin A1 (to block the lysosomal turnover of LC3-II) for 3 hrs and subjected to immunoblotting using the mCherry antibody (Abcam, ab125096) with a ratio of 1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... cells were blocked in 3% -BSA/1x PBS for 30 min prior to incubation with fluorescence-conjugated Phalloidin (abcam, Cat# ab176753) at 1:000 for 1 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... was incubated at 40C overnight followed by application of a rabbit-specific HRP/3-amino-9-ethylcarbazole (AEC) IHC Detection Kit - Micro-polymer (abcam, ab236468) incubated at room temperature for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in PBS with 3% donkey serum and the following primary antibodies for 72 h at 4°C: goat anti-Iba1 (1:500; Abcam, ab5076), rabbit anti-ACSL1 (1:100 ...
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bioRxiv - Microbiology 2023Quote: 10 µm-thick paraffin sections from juvenile and adult Chinook salmon brains (n=3/group) were deparaffinized and treated with trypsin antigen retrieval solution (Abcam, ab970) at room temperature for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked in TBS containing 3% BSA for 1h at RT and incubated with rabbit anti-B7H3 (1:1000, clone EPNCIR122, Abcam, ab134161) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... washed twice with 3 % BSA and incubated for 1 h with monoclonal rabbit anti-Ki-67 antibody (Abcam, ab16667, 1:1000) and washed three times with PBS ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 30ng of this extract was used for subsequent immunoprecipitation using 4μl of antibody targeting H3K4me3 (Abcam AB8580 lot GR273043-3).
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bioRxiv - Immunology 2024Quote: ... (Cell-Signaling Technology, 2148), Cleaved caspase-3 (CST, 9661S), Cleaved caspase-8 (CST, 8592S), IL-18 (1:1000, WB) (Abcam, ab207323), GSDMC2/GSDMC3 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3 times in TBST and incubated with the secondary antibody (donkey anti-rabbit IgG HRP conjugate, AbCam 16284) diluted 1/5000 in TBST for 2 h at room temperature ...
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Varying recombination landscapes between individuals are driven by polymorphic transposable elementsbioRxiv - Genomics 2024Quote: ... We followed the CUT&Tag@home protocol (V.3, (Kaya-Okur et al. 2019; Henikoff et al. 2020)) using H3K9me3 rabbit antibody (Abcam EPR16601) and anti-rabbit Secondary Antibody binding (EpiCypher 13-0047) ...
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bioRxiv - Cancer Biology 2024Quote: ... The assay plate was washed 3 times with 100 µl/well of PBST and 20 µl/well biotinylated detection antibody (Abcam, Ab62352) was diluted (1:100 in dilution buffer ...
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bioRxiv - Genetics 2024Quote: ... washed 3×10 minutes in TBS-T and then blocked for an hour in TBS-T+ (TBS-T with 3% normal goat serum (Abcam, ab7481)) at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Changes in mitochondrial membrane potential produced by I3C and BNF in the five cancer cell lines was determined with the tetramethylrhodamine ethyl ester (TMRE) Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescently labelled detection antibodies (AT8: Invitrogen, Cat. No. MN1020, 5 nM; HT7: Invitrogen, Cat. No. MN1000, 2 nM; T181: abcam, Cat. No. ab232849, 5 nM) were added in blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... Ten milliliters of 1x PBST + 5% blotting grade blocker with 2 μL anti-mouse IgG1 (Abcam) were added and incubated for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: EdU staining was performed by incubating larvae in 1 mM EdU (5-ethynyl-2′- deoxyuridine) (Abcam) in 1 % DMSO (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Non-decalcified E16.5 and P0 samples had mineralized bone visualized by von Kossa staining (n=3 per age and genotype) with Nuclear Fast Red counterstaining as part of an established protocol (Abcam, Cambridge, UK). For osteoclast staining ...