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Citations for abcam :
551 - 600 of 2638 citations for 3 Ethyl 3 methyloxolane 2 5 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Brain floating slices were incubated 3 days at 4°C in the blocking condition with primary antibody chicken anti-EGFP (1:1,000; ab13970, Abcam). After three PBS washes ...
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bioRxiv - Genetics 2020Quote: Mitochondrial membrane potential was measured by flow cytometry in HEK293 cells using TMRE (Tetramethylrhodamine, Ethyl ester, Abcam, ab113852). FCCP was used as a positive control to validate each independent experiment ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed three times in 1XPBS for 5’ and incubated with anti-neural/glial antigen 2 (NG-2, 1:100, in 1XPBS + 1%NGS, Abcam, Cambidge, UK), overnight at 4°C.
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bioRxiv - Evolutionary Biology 2020Quote: ... then washed the 3 times in 1XPBS 5min at RT and incubated with the secondary antibody (1:200, Abcam ab175471) for 2-3h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... the 5’ adapter (containing four random nucleotides at the 3’ end) was ligated to the small RNAs using T4 RNA ligase (Abcam) for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of antibody per IP (Cas9 – Diagenode C15310258; MRE11 – Novus NB100-142; γH2AX – Abcam ab81299; 53BP1 – Novus NB100-305) was added and placed on rotator for 1-2 h ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% dry milk and incubated overnight at 4°C with rabbit polyclonal antibody against p-Smad2/3 (1:200; ab272332, Abcam), Smad2/3 (1:200 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were blocked with 1% BSA-0.3 M glycine in PBST and incubated with mouse anti-human integrin αvβ3 IgG (1:200, monoclonal, LM609, Abcam, ab190147) in 1% BSA-PBST overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Immunostaining was performed with specific primary antibodies (anti-TH, anti-GFAP, anti-NG2, anti-TMEM119, anti-pSer129-αSyn, anti-Cleaved Caspase-3, anti-CD68; Abcam), (anti-MAP2 ...
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bioRxiv - Biochemistry 2020Quote: ... 0.05% Triton-X 100) and subsequently incubated O/N with primary antibodies (3-pHis: rabbit, SC44-1 and F4/80: rat, ab6640; abcam) diluted in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and M2 activated (n = 3) was reported as fold-change from control using a commercial intracellular ROS fluorescence assay kit (Abcam, used according to manufacturer’s instructions) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed and permeabilized before incubation with the FITC-conjugated caspase-3 antibody following manufacturer’s instructions for subsequent flow cytometry analysis (Abcam, #65613). To assess membrane lipid perodixation ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with 10 μM TG100-115 for 3 days and conditioned medium was collected as described above followed by ELISA assay using human IL11 ELISA kit (Abcam).
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bioRxiv - Systems Biology 2021Quote: ... cells were incubated with 3% BSA and 0.1% Triton X-100 for 30 min at RT and stained with anti-gamma H2AX (Abcam 2893), anti CENP B (Santa Cruz ...
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bioRxiv - Biochemistry 2022Quote: ... Then membranes were washed (3×) with 1 × PBST for 10 min each wash and incubated with anti-Rabbit (diluted 1:5000, ab6721, Abcam) or anti-Mouse (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... for 6 or 20 hours before inducing degradation of GRASP55 and/or GRASP65 by addition of 500 μM indole-3-acetic acid (IAA, Abcam). The Golgi ribbon was disassembled into single stacks by treatment with 3 μg/ml nocodazole (EMD Millipore ...
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bioRxiv - Genomics 2021Quote: ... 30 µg of proteins were used for each western blot and proteins were detected using anti-SUMO2/3 (ab3742, Abcam) and anti-TBP (ab51841 ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 3 times in 0.1% Tween-20 in TBS and incubated overnight at 4C with antibodies to: MHC II (Abcam ab55152), TZAP (Abnova H0003-B01P) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3’- diaminobenzidine (DAB) were done using the Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466, Abcam). Before the protein block ...
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bioRxiv - Microbiology 2023Quote: ... Caspase activation was assayed with the Caspase-3 and Caspase-1 colorimetric assay kits from abcam (ab39401 and ab273268, respectively). At harvest ...
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bioRxiv - Genetics 2023Quote: ... Chromatin from 250,000 nuclei of each cell sub-populations (n=3) was isolated and used for ChIP with anti-H3K27ac and anti-H3 antibodies (Abcam) using the iDeal ChIP-seq kit (Diagenode) ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then incubated for 3 days at 32°C with an appropriate combination of primary antibodies to TNNI3 (ab56357, Abcam; diluted 1:200 to a concentration of 5 µg/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... The equivalent of 3-6 x107 cells were precipitated overnight with 1 ug IgG/106 cells of rabbit antibody against H3K27ac (ab4729, Abcam). Before antibody precipitation ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were further rinsed with PBS for 3 times 10 min each and mounted with mounting medium with DAPI (Abcam).
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bioRxiv - Evolutionary Biology 2023Quote: ... the brains were washed for 3 x 10 minutes in PBST and incubated with 1:200 goat anti-mouse IgG (ab150115, abcam) in 5 % NGS for 48 hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... washed 3 times with PBS and incubated with anti-mouse secondary antibodies conjugated with Alexa647 (ab150107, Abcam, 1:1000 dilution) or Alexa488 (A21202 ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were probed overnight at 4℃ in 1% milk in TBST with the following antibodies: rabbit anti-14-3-3ε (1:1000) (Abcam); rabbit anti-Myc (1:1000 ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... which were blocked with 3% BSA in TBS containing 0.2% tween-20 (TBS-T) and probed with rαstrep (Abcam, polyclonal, ab76949), overnight at 4°C with gentle rocking ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times in PBS then incubated with Alexa 488-conjugated goat anti-rabbit secondary antibody (1:500; ab150077, Abcam) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The brain sections were washed 3 times with PBS solution before being immersed with chicken anti-GFP antibody (1:500; ab13970, Abcam) for 12 hours at 4°C ...
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bioRxiv - Physiology 2024Quote: ... and diphenyleneiodonium (DPI, to block ROS production; 3 µM for 60 min, from a 10 mM stock in DMSO, Abcam).
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bioRxiv - Cancer Biology 2023Quote: ... Magnetics beads (FischerScientific, 13484209) were coated during the same time with 3 µg of the following antibodies: TRIM28 (Abcam, ab10484), YARS (SantaCruz ...
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bioRxiv - Neuroscience 2023Quote: ... Brain slices containing basal forebrain were incubated in primary antibody (1:500 dilution of 3% normal goat serum in 1% Triton X100 dissolved in PBS of chicken anti-GFP IgY, Abcam catalog # ab13970 and rabbit anti- mCherry IgG ...
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bioRxiv - Biochemistry 2022Quote: SISA strains KS8266 and KS8311 (Mustavchiev et al., 2016) were maintained on Ye5S media containing 5μM 3-BrB-PP1(Abcam, ab143756) to prevent activation of sty1 and retention of Wis1DD was confirmed by sequencing of cells used for experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... SMARCA4 ChIP was performed using 20 µg of chromatin incubated with 3 µg of SMARCA4 antibody (ABCAM, Cambridge, UK 110641). Digested chromatin and antibody were incubated overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then rinsed again in PBS (3 x 10 min) and treated with secondary antibody [Alexa fluor 594-conjugatedanti-rabbit IgG antibody (Abcam), 1:500] for 2 hours at 4°c ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then blocked using 3% bovine serum albumin (BSA) for 1 hour and incubated with anti-V5 primary antibody (1:2000; Abcam) in 1x PBS and 10 % normal goat serum overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Brain floating slices were incubated 3 days at 4°C with primary antibodies: chicken anti-EGFP (1:1000; ab13970, Abcam), rabbit anti-TBR1 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were transferred to PVDF membranes and then immunoblotted with the following antibodies: cleaved Caspase-3 (1:500, Abcam, ab13847), WAVE1 (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... endogenous peroxidase inactivation with 3% H2O2 was performed on dewaxed specimen followed by blocking with avidin biotin blocking solution (ab64212, Abcam) and 5% BSA in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS-TX (3 x 5min) and incubated in PBS-TX containing secondary goat anti-rabbit AlexaFluor 488 (1:500 dilution, Ab150077, Abcam) or goat anti-mouse AlexaFluor 488 (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... the slides were washed with PBST five times for 3 min each and incubated with the secandary antibody (Goat Anti-Mouse IgG H&L [ab205719, Abcam]) (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... X31 at an MOI of 3 was added to cells for one hour prior at 4 degrees in the presence of 1 mM cycloheximide (Abcam). Unbound bound virus was then removed with 3 washes of ice-cold PBS prior to the addition of infection media ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibody solutions were prepared in TBS++ (TBS, 0.1% Triton X-100 and 3% donkey serum) against MAP2 1:2000 (Abcam; ab5392), LMX1A (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked in 5% normal serum in 1× PBS Triton 0.3% for 1 hr at room temperature and then incubated with the primary antibody (anti-S100b diluted 1:1000, Abcam, ab5264 or anti-RPE65 diluted 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS (3×10 min) and incubated with Donkey anti-Rabbit Alexa Fluor 647 (1:1000, ab150063, abcam) secondary antibody diluted in a solution containing 5% normal donkey serum and 0.3% Triton X-100 in PBS for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were post-fixed on slides with 4% paraformaldehyde before being permeabilized with 3% TritonX-100 and immunostained with IBA1 (AbCam), NeuN (ThermoFischer) ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 3 μg of the peptide was loaded on an SDS–polyacrylamide gel and stained with InstantBlue Coomassie Protein Stain (Abcam). Intensity of the remaining peptide bands were quantified using the ImageJ and plotted.
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bioRxiv - Neuroscience 2024Quote: ... the slides were washed in PBS for 3 times and incubated with goat anti-mouse Alexa Fluor 488 secondary antibody (1:250, Abcam) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: Competition-EMSA was done as described previously (Jin, et al. 1999) with JEG-3 cell nuclear extract isolated using the EpiSeeker nuclear extraction kit (Abcam). Nuclear extract (3 μg) ...