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Citations for abcam :
601 - 650 of 657 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... every fourth section throughout the hippocampus/dentate gyrus was collected and stained with panel 3 = anti-mouse BrdU (ab8152, Abcam, 1:500), anti-mouse doublecortin (DCX ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The cultures subsequently underwent 3 cycles of 10 min PBS washes and incubated for 40 min with the appropriate secondary antibodies (Abcam or Jackson) and DAPI ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies (all diluted in 3% BSA in PBS) were used at the final concentration indicated: chicken anti-GFP (ab13970 from Abcam, 1:5000), rabbit anti-mCherry (GTX128508 from Genetex ...
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bioRxiv - Molecular Biology 2022Quote: ... NAD(P)H quinone dehydrogenase 1 (NQO-1; #ab80588) and pan-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #ab8245) antibodies were provided by Abcam (Cambridge, England). The p38 MAPK inhibitor SB203580 (#S8307 ...
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bioRxiv - Microbiology 2020Quote: ... 1:300 dilution of mouse anti-PfDHODH #3 (a kind gift from Margeret Phillips, University of Texas Southwestern) or 1/5000 mouse anti-PfAldolase-HRP (Abcam, Cat #ab38905). The next day ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured with 2-deoxy-2-((7-nitro-2, 1, 3-benzooxadiazol-4-yl)amino)-glucose (2-NBDG) (Abcam 50μM, 1h), 4 ...
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bioRxiv - Immunology 2022Quote: ... albicans hyphae for 3 hours and then labelled with anti-histone H3 citrulline R2+R8+R17 (Abcam, Cambridge, MA; 0.014 mg/ml) followed by donkey anti-rabbit IgG Cy3 (Jackson Immunoresearch ...
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bioRxiv - Microbiology 2021Quote: ... Consecutive sections were processed for immunohistochemical staining with reovirus-specific rabbit polyclonal antiserum (1:1000) or activated caspase-3 (Abcam, 1:100). Images were taken on an EVOS FL Auto 2 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times in PB and were mounted on a glass slide in the mounting medium with DAPI (Abcam ab 104139). The slides were imaged at 20X on a Zeiss LSM 880 airyscan confocal microscope.
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bioRxiv - Physiology 2021Quote: ... Next day the sections were washed 3 × 10 minutes with PBS and stained with an anti-rabbit secondary antibody (1:1000, AB150077, Abcam, Alexa 488) in 2% BSA in PBS for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was re-suspended with 0.9% of sodium chloride solution and reacted with a rabbit anti-mouse HMGB1 primary antibody for 3 hrs at room temperature (1:350, abcam, Cat. #ab18256), followed by a goat anti-rabbit secondary antibody conjugated with Cy3 for 1hr at room temperature (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... 100-200 μg of precleared chromatin was incubated at 4°C for three hours with magnetic beads that had been prebound to one of the following antibodies: 3 μg of α-V5 antibody (Abcam ab9116), 1.5 μg of α-H3K9me2 antibody (Abcam ab1220) ...
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bioRxiv - Physiology 2022Quote: ... washed with PBS-T 3 times for 10min and incubated O/N at 4°C with primary antibody (Rabbit anti-Calnexin, Abcam, Cat# ab133615) at a dilution of 1:1000 in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... samples were blocked in PBTx (1% Triton X-100 in PBS) containing 3% bovine serum albumin and incubated over three nights with chicken anti-GFP antibody (Abcam Cat#ab13790). After several washes in PBTx ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibodies were diluted in 3% BSA/PBS and incubated overnight at 4°C: rabbit anti-RPA34 (Abcam ab97594, 1:1000), mouse anti-PML (Santa Cruz Biotechnology sc-966 ...
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bioRxiv - Microbiology 2023Quote: ... bound complexes were isolated by overnight precipitation of the pre-cleared chromatin with 100 µL of blocked beads in combination with 3-4 µg of antibodies (anti-Histone H3 (Abcam ab17913, µg), Histone H3Ac (Millipore 17-615 ...
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bioRxiv - Neuroscience 2023Quote: ... Brain slices containing locus coeruleus and NTS were incubated in primary antibody (1:500 dilution of 3% normal goat serum in 1% Triton X100 dissolved in PBS, rabbit anti-mCherry IgG, Abcam catalog # ab167453) for 24-48 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in 2x SSC (3 x 5 min) and incubated in blocking solution containing secondary antibody (anti-chicken Alexa 488, Abcam, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the sections were washed 3 more times in PBS followed by incubation in the secondary antibody (antirabbit Alexa Fluor-488, Abcam; Sydney, Australia) diluted in blocking solution (1:250 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were then incubated overnight at 4°C with the following primary antibodies diluted in PBS 3% BSA 0.2% Triton X100: anti-β-Tubulin (clone 2A1A9, dilution 1/200, Abcam, Cambridge, UK), anti-YAP1 (clone D8H1X ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were washed 3 times with 1X PBST before adding anti-Rabbit secondary antibody (Catalog No: ab150080, Abcam, Waltham, MA, USA) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... The sections were blocked with 10% goat serum/3% BSA/0.3% Triton X-100 and stained with antibodies to Netrin-1 (Abcam #ab126729, 1:200), Cytokeratin (human specific cytokeratin 7 and 8 ...
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bioRxiv - Immunology 2024Quote: ... The resulting lysates was quantified using cytokine specific ELISA kits (IL-18 Thermo Fisher #BMS618-3 and IL-18 BP Abcam # ab254509), and absorbance was measured on the Tecan Spark 10M (Tecan ...
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bioRxiv - Neuroscience 2024Quote: ... as reported.9 Tissue sections were then permeabilized with 0.3% Triton X-100 for 10 min at RT and incubated with rabbit polyclonal anti-PSD95 (1:250, ab18258, Abcam, Cambridge, UK) overnight at 4°C ...
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bioRxiv - Pathology 2024Quote: ... The sections were blocked with PBS / 3% BSA for 30min and sequentially immunostained with the primary rabbit anti-α-SMA antibody (Abcam, #ab5694) and the corresponding secondary HRP-conjugated goat anti-rabbit IgG antibody (Genetech ...
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bioRxiv - Immunology 2024Quote: ... before blocking with Blocking buffer (PBS with 10% normal donkey serum and 3% BSA) followed by overnight incubation with rabbit antimouse CD4 (abcam, Cat# ab183685) and rat antimouse Ly-6G (clone 1A8 ...
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bioRxiv - Cell Biology 2024Quote: ... primary antibody incubation was performed with DNA/RNA damage antibody (8-OHdG; Novus, Littleton, CO, USA) or cleaved caspase-3 (Abcam, Cambridge, UK) (both at 5 µg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Chromatin (125 µg/IP) was incubated overnight in 2 µg of either rabbit polyclonal anti-histone 3 lysine 4 trimethyl (H3K4me3; Abcam, Cat# ab8580, RRID:AB_306649) or anti-Histone 3 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... following which sections were blocked in 3% normal horse serum (NHS) for 30min before overnight incubation with Tyrosine hydroxylase (TH) (Abcam, ab112, 1:750) or IBA-1 (Wako ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in 1x PBS for 10 minutes then incubated in blocking solution containing AlexaFlour488 conjugated secondary antibodies (Abcam, ab150077; 1:1000) for 2-4 hours at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
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bioRxiv - Bioengineering 2023Quote: ... The slices were then incubated overnight at 4°C with primary antibody solutions (supplemented with 3% (v/v) Triton X-100 in 1× PBS) to target astrocytes (GFAP) and neuronal nuclei (NeuN) (Abcam Inc., Cambridge, UK) (Table 1) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... The same procedure was used for flat-mounts but primary incubation was for 3 days and secondary incubation for 1 day using anti-GFP 1:500 (Abcam, Cambridge, UK; Chk).
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... gravid worms were placed in 1 drop of ddH2O containing 3% (v/v) pelleted OP50 and 100 μM bafilomycin A1 (Abcam, dissolved in DMSO) for 16 h at 20 ℃ ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for western blotting at the indicated dilutions: anti-CD63 (MBL, D263-3, 1:5000; Abcam, ab134045, 1:2000), anti–Flotilin-1 (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were incubated for 3 h at room temperature with a mix of secondary antibodies (ThermoFisher A-11039 or Abcam ab150158, 1:400) that had been previously centrifuged at 20000g for 10 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Apoptosis and proliferation levels were measured by immunostaining in dewaxed and rehydrated midline placental sections with cleaved caspase-3 (Asp175) (Cell Signalling, US, 9661; 1:200) and Ki67 (Abcam, UK, ab264429; 1:500). Sections were incubated with goat-anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing both WGA-555 (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times with 1x PBS and placed in secondary (1:500 AF488 conjugated Goat anti-Rabbit (Abcam, Cambridge, UK, Cat# AB150077), 1:500 Cy3 conjugated Donkey anti-mouse (Jackson Immuno Research Labs ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then treated with 3% bovine serum albumin in PBS and incubated overnight at 4°C with the primary mouse antibody anti-Pax8 (Abcam ab53490, diluted 1/100). After washing unbound antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... The lower part of the membrane was blocked with 3% BSA in phosphate-buffered saline (PBS) solution followed by incubation with rabbit polyclonal anti-GFP (Abcam, UK; #ab290, 1:1,000) primary antibody at RT for 1 h for eGFP detection (30 kDa) ...
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bioRxiv - Neuroscience 2023Quote: ... Following primary antibody incubation brains were washed 4 times for 15 minutes each in a 3% salt solution (in 1X PBST) and transferred to secondary antibodies (Abcam: ab175673; ThermoFisher: A-21236) in 1XPBST and incubated overnight at 4°C on a nutator ...
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bioRxiv - Neuroscience 2024Quote: Western blot analysis was performed on hCMEC/D3 cell lysates using antibodies against full-length and cleaved caspase-3 (Abcam, ab32351, 1:500 dilution), VE-Cadherin (Cell Signalling ...
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bioRxiv - Neuroscience 2024Quote: ... except that brains were incubated overnight in apoptosis-related primary antibodies (H2A.X, 1:500, EMD Millipore, 05-636, made in mouse; and caspase 3, 1:500, abCam, AB13847, made in rabbit) overnight in blocking buffer at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were washed in TBS-T for 3 × 10 minutes incubating with secondary anti-rabbit (Cell Signalling Technology catalogue # 7074S; 1:2,000) and anti-mouse (Abcam Ltd. Catalogue # ab6728; 1:5,000) in TBS-T with 5% milk for one hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections at the level of the PnC of GlyT2-eGFP mice injected with pAAV DJ-CamKIIα-mCherry in the CeA (N=6 mice) were incubated with a chicken anti-mCherry (1:1000, Abcam, catalog# ab205402, lot# GR225123-3) and a rabbit anti-PSD95 (1:500 ...