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Citations for abcam :
451 - 500 of 657 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Primary antibodies were as follows: mouse anti-talin1 (clone 93E12, Abcam: ab104913, Lot#: GR24449-3, 0.5 μg/ml), rabbit anti-zyxin (B71 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mL saponification buffer containing 7% KOH in 92% ethanol with 10 ug/mL coprostan-3-ol (Abcam) was added to 900 μL cell lysate ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 3 times with PBST and then incubated with a goat anti-human HRP (abcam ab7153) secondary ...
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bioRxiv - Cell Biology 2021Quote: ... Caspase-3, Abcam, rabbit, 1:500; CHOP, Abcam, rabbit, 1:1,000; ATF4, Abcam, rabbit, 1:1,000; GRP78, Abcam, rabbit ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were incubated overnight at 4°C with the following primary antibodies: Syntaxin 3 (Abcam, cat. no. 133750), TRPML1 (Novus Biologicals ...
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bioRxiv - Cell Biology 2023Quote: ... GRASP65 was then depleted by treatment with 0.5 mM of the auxin indole-3-acetic acid (IAA, Abcam) for 2 h (Zhang and Seemann ...
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bioRxiv - Neuroscience 2023Quote: ... Precleared lysates (equal amounts of protein) were incubated with 3 µg rabbit polyclonal antibody to NrCAM (Abcam #24344) or nonimmune IgG (nIg ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were then washed in PBS 3 times then developed using the DAB substrate kit (Abcam #ab64238). The slides were covered in hematoxylin for 3 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were blocked with 3% BSA in TBS and stained with a rabbit anti-RBM20 antibody (Abcam, #ab233147) at 10 μg/ml for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies in the study were used at a concentration of 1:1000 and include: VDAC1/3 (Abcam, ab14734), MCU (Cell Signal ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4 °C with either rabbit anti-TAF1 exon 3 (1:500; Abcam, ab188427), rabbit anti-TAF1 exon 37 (1:500 ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Slides were subsequently blocked with blocking buffer (3% natural serum, 1% BSA, 0.1% Tween-20) and incubated overnight with anti-EZH2 antibody (3748; Abcam), anti-CREB3L1 (AF4080 ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) primary antibodies (Catalog NO: ab181602) were purchased from Abcam (Cambridge, MA, USA). Other chemicals were of analytical grade and used without further purification.
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody dilutions were prepared in 3% NGS in 1XPBS as follows: mouse anti-human vinculin (VIN-54, Abcam) at 1:200 and phalloidin conjugated with Alexa Fluor 546 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated overnight at 4 °C in a mixture of rabbit anti-Cav-3 (1:100, ab2912, Abcam) antibody and a custom rabbit anti-NCX1 antibody as previously described5 (1:100 ...
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bioRxiv - Neuroscience 2021Quote: Slices were placed into an open bath chamber and perfused constantly with aCSF (2-3 mL/min) supplemented with tetrodotoxin (0.5 μM; Abcam) and picrotoxin (50 μM ...
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bioRxiv - Neuroscience 2020Quote: ... Germany) and 0.5 ng/μl anti-Atxn3-(N-terminal)-tb (rabbit-anti-Ataxin-3, ab96316, Abcam, Cambridge, United Kingdom) were mixed in 1x detection buffer (in 50 mm NaH2 PO4 ...
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bioRxiv - Neuroscience 2020Quote: Cathepsin B activity was assayed in the cortex of 3-month old mice using a fluorometric kit (Abcam, #ab65300). Tissue was homogenised in lysis buffer then incubated for 30 minutes on ice ...
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bioRxiv - Neuroscience 2020Quote: Cell viability was measured after 3 days and 5 days of culture using an MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (Abcam, ab211091) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Levels of NT3 protein in serum and muscles were assessed using a Human Neurotrophin-3 ELISA Kit (#ab100615 Abcam). In this study the viral vector encoded the human prepro-NT3 gene ...
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bioRxiv - Microbiology 2022Quote: The cytotoxic effect of Gal-9 on Calu-3 cells was determined using an MTT assay kit (abcam, ab211091) according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... and the obtained values represent the ratio between the immunodetected protein and the glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Abcam) loading control ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times with TBS-T and primary 4-HNE antibody was added at 1:200 dilution (Abcam, #ab46545). The antibody was left on overnight at 4°C rocking ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight in 3% normal donkey serum and 0.3% PBST containing primary antibodies for GFP (1:2000, chicken anti-GFP, ab13970, Abcam), and/or TH (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were blocked in 3% PBS-BSA with 0.05% Tween-20 for 15 minutes and then incubated in primary antibody (NuMa: Abcam ab109262 ...
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bioRxiv - Biophysics 2024Quote: ... Attached cells were then fixed by methanol/acetic acid solution (3:1) stained with Perl’s Prussian blue with nuclear fast red as counterstain (Iron stain kit, ab150674, abcam). Slides were dehydrated (incubation with 75 ...
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in 0.3% PBT and incubated with secondary antibodies and Hoechst (Abcam) in 3% NDS in 0.3% PBT for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... recovery aCSF included (R,S)-3-(2-Carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP, 5 μM; Abcam, ab120160) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline (NBQX ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h at room temperature in 20 mL TBS-T with 3% milk powder and 1:5,000 diluted anti-GFP antiserum (Abcam) while shaking with 180 rpm ...
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bioRxiv - Immunology 2023Quote: ... ANKRD22 (Cat# ab11638) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat# ab 9485) were obtained from Abcam (Waltham, MA, USA). Anti-Stabin-1 (Cat# AB AB6021 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated overnight at 4°C with 1:500 diluted anti-14-3-3β antibody (abcam ab15260) in 5% BSA/TBST ...
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bioRxiv - Neuroscience 2023Quote: ... the sectioned slices were incubated for one hour at room temperature in blocking solution (10% normal goat serum and 0.3% Titron X-100 in 1x TBS) and incubated overnight in polyclonal rabbit anti-HA (1:1,000, #ab9110, Abcam) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 x 5min with TBST and placed in HrP-linked rabbit anti-mouse secondary antibody (Abcam) (Ab diluted to 0.5μg/mL in TBST for 2h at RT) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight in primary antibodies then washed (3 x 0.1M PBS) and incubated in secondary antibodies conjugated to Alexa-594 (1:200, Abcam) or Alexa-488 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... 5 µg/ml rat anti-mouse C3b/iC3b (clone 3/26, Hycult Biotec) and 5 µg/ml rabbit anti-mouse C1q (clone 4.8, Abcam). Secondary antibodies were added for 3h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Brain floating slices were incubated 3 days at 4°C in the blocking condition with primary antibody chicken anti-EGFP (1:1,000; ab13970, Abcam). After three PBS washes ...
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bioRxiv - Genetics 2024Quote: ... Standard 5 µm sections were prepared and stained for eosinophils (Anti-Ribonuclease 3/eosinophil cationic protein antibody, Abcam, Ab207429). The presence of ECRSwNP was determined by counting the number of eosinophils per high-power field (HPF) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then washed the 3 times in 1XPBS 5min at RT and incubated with the secondary antibody (1:200, Abcam ab175471) for 2-3h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated for 10 min at 37 °C in the presence of 10 µM 2-amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile (UCPH-101; Abcam 120309, UK), a specific excitatory amino acid transporter 1 (EAAT1/Glast ...
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bioRxiv - Molecular Biology 2021Quote: ... the 5’ adapter (containing four random nucleotides at the 3’ end) was ligated to the small RNAs using T4 RNA ligase (Abcam) for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of antibody per IP (Cas9 – Diagenode C15310258; MRE11 – Novus NB100-142; γH2AX – Abcam ab81299; 53BP1 – Novus NB100-305) was added and placed on rotator for 1-2 h ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% dry milk and incubated overnight at 4°C with rabbit polyclonal antibody against p-Smad2/3 (1:200; ab272332, Abcam), Smad2/3 (1:200 ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed by incubation of 2 ml diluted chromatin (equivalent to 5×106 input cells) with antibodies for H3K4me1 (3 µg ab195391, lot: GR304893-2; AbCam), H3K4me3 (1 µl 07-473 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were blocked with 1% BSA-0.3 M glycine in PBST and incubated with mouse anti-human integrin αvβ3 IgG (1:200, monoclonal, LM609, Abcam, ab190147) in 1% BSA-PBST overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Immunostaining was performed with specific primary antibodies (anti-TH, anti-GFAP, anti-NG2, anti-TMEM119, anti-pSer129-αSyn, anti-Cleaved Caspase-3, anti-CD68; Abcam), (anti-MAP2 ...
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bioRxiv - Biochemistry 2020Quote: ... 0.05% Triton-X 100) and subsequently incubated O/N with primary antibodies (3-pHis: rabbit, SC44-1 and F4/80: rat, ab6640; abcam) diluted in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and M2 activated (n = 3) was reported as fold-change from control using a commercial intracellular ROS fluorescence assay kit (Abcam, used according to manufacturer’s instructions) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed and permeabilized before incubation with the FITC-conjugated caspase-3 antibody following manufacturer’s instructions for subsequent flow cytometry analysis (Abcam, #65613). To assess membrane lipid perodixation ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with 10 μM TG100-115 for 3 days and conditioned medium was collected as described above followed by ELISA assay using human IL11 ELISA kit (Abcam).