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Citations for abcam :
601 - 650 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: SISA strains KS8266 and KS8311 (Mustavchiev et al., 2016) were maintained on Ye5S media containing 5μM 3-BrB-PP1(Abcam, ab143756) to prevent activation of sty1 and retention of Wis1DD was confirmed by sequencing of cells used for experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... SMARCA4 ChIP was performed using 20 µg of chromatin incubated with 3 µg of SMARCA4 antibody (ABCAM, Cambridge, UK 110641). Digested chromatin and antibody were incubated overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then rinsed again in PBS (3 x 10 min) and treated with secondary antibody [Alexa fluor 594-conjugatedanti-rabbit IgG antibody (Abcam), 1:500] for 2 hours at 4°c ...
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bioRxiv - Cancer Biology 2024Quote: ... endogenous peroxidase inactivation with 3% H2O2 was performed on dewaxed specimen followed by blocking with avidin biotin blocking solution (ab64212, Abcam) and 5% BSA in TBST ...
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bioRxiv - Developmental Biology 2024Quote: ... the slides were washed with PBST five times for 3 min each and incubated with the secandary antibody (Goat Anti-Mouse IgG H&L [ab205719, Abcam]) (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were post-fixed on slides with 4% paraformaldehyde before being permeabilized with 3% TritonX-100 and immunostained with IBA1 (AbCam), NeuN (ThermoFischer) ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 3 μg of the peptide was loaded on an SDS–polyacrylamide gel and stained with InstantBlue Coomassie Protein Stain (Abcam). Intensity of the remaining peptide bands were quantified using the ImageJ and plotted.
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bioRxiv - Molecular Biology 2024Quote: Competition-EMSA was done as described previously (Jin, et al. 1999) with JEG-3 cell nuclear extract isolated using the EpiSeeker nuclear extraction kit (Abcam). Nuclear extract (3 μg) ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed 3 times for 5 min with PBS before incubating with a secondary antibody (ab150080 and ab150157, Abcam) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and then mechanically dissociated by repeated pipetting and filtered through a 40-μm nylon mesh prior to adding 3 nM of DRAQ-7 (Abcam) for viability assessment for flow cytometry analysis and/or sorting ...
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bioRxiv - Developmental Biology 2024Quote: ... starting from 3-5x106 cell for TFs and 0.5-1x106 for H3K27ac and using the following antibodies: MEIS1( Abcam ab19867), GATA-6 XP Rabbit mAb (D61E4 ...
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bioRxiv - Genetics 2021Quote: ... were incubated in blocking solution (0.5% bovine serum albumin in PBS) with 3 μg of antibody to histone-H3 K27ac (Abcam, cat. # ab4729) for 3 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... In the next step the cells were washed with 3% BSA solution and incubated with 1x Phalloidin-iFluor594 (Abcam, stock 1000x) solution (in 1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: Beads that were incubated with FLI 7.3 mouse antibody were resuspended in 100 µL Dig-wash buffer and incubated with rabbit anti-mouse secondary antibody (Abcam, ab46540) at a dilution of 1:100 on a rotator for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 47 µl of Inside Perm Solution and stained with 3 µl of Anti-PBR antibody [EPR5384] (abcam, ab199836). After 10 minutes of incubation ...
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bioRxiv - Biophysics 2024Quote: ... The micro-tissues were then permeabilized in 0.5% Triton X-100 in PBS for 5 min and blocked in 3% bovine serum albumin (BSA) in PBS before they were incubated with primary antibodies (anti-sarcomeric alpha actinin (Abcam #9465) or desmin (Cell Signaling #5332) ...
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bioRxiv - Microbiology 2023Quote: 10 µm-thick paraffin sections from juvenile and adult Chinook salmon brains (n=3/group) were deparaffinized and treated with trypsin antigen retrieval solution (Abcam, ab970) at room temperature for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing goat anti-rabbit IgG H&L Alexa Fluor 488 (abcam; ab150077).
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bioRxiv - Cell Biology 2022Quote: ... log-phase liquid cultures were divided in two and incubated for different times in YES-Glucose medium treated with 10 µM 3-BrB-PP1 (Abcam, ab143756), or in medium lacking the analog kinase inhibitor ...
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bioRxiv - Biochemistry 2022Quote: Cells were incubated in complete media in the presence of 100 nM bafilomycin A1 (to block the lysosomal turnover of LC3-II) for 3 hrs and subjected to immunoblotting using the mCherry antibody (Abcam, ab125096) with a ratio of 1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS for 5 minutes and incubated with AlexaFluor® 647 conjugated secondary antibodies (Ab150079, Ab150115; Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were blocked in 3% -BSA/1x PBS for 30 min prior to incubation with fluorescence-conjugated Phalloidin (abcam, Cat# ab176753) at 1:000 for 1 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... was incubated at 40C overnight followed by application of a rabbit-specific HRP/3-amino-9-ethylcarbazole (AEC) IHC Detection Kit - Micro-polymer (abcam, ab236468) incubated at room temperature for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... and 30ng of this extract was used for subsequent immunoprecipitation using 4μl of antibody targeting H3K4me3 (Abcam AB8580 lot GR273043-3).
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3 times in TBST and incubated with the secondary antibody (donkey anti-rabbit IgG HRP conjugate, AbCam 16284) diluted 1/5000 in TBST for 2 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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Varying recombination landscapes between individuals are driven by polymorphic transposable elementsbioRxiv - Genomics 2024Quote: ... We followed the CUT&Tag@home protocol (V.3, (Kaya-Okur et al. 2019; Henikoff et al. 2020)) using H3K9me3 rabbit antibody (Abcam EPR16601) and anti-rabbit Secondary Antibody binding (EpiCypher 13-0047) ...
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bioRxiv - Cancer Biology 2024Quote: ... The assay plate was washed 3 times with 100 µl/well of PBST and 20 µl/well biotinylated detection antibody (Abcam, Ab62352) was diluted (1:100 in dilution buffer ...
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bioRxiv - Genetics 2024Quote: ... washed 3×10 minutes in TBS-T and then blocked for an hour in TBS-T+ (TBS-T with 3% normal goat serum (Abcam, ab7481)) at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Non-decalcified E16.5 and P0 samples had mineralized bone visualized by von Kossa staining (n=3 per age and genotype) with Nuclear Fast Red counterstaining as part of an established protocol (Abcam, Cambridge, UK). For osteoclast staining ...
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bioRxiv - Bioengineering 2020Quote: ... Permeabilized cells were rinsed 3 times with 1X PBS and incubated in 1X PBS with primary antibody Rabbit-α-Tomm20 (Abcam, ab186735) and Mouse-α-LaminA/C (Abcam ...
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bioRxiv - Physiology 2020Quote: ... Cells were washed 3 × in TBS and incubated overnight (4°C) in 300 μl of anti-desmin (Abcam Cat# ab15200, RRID: AB_301744) primary antibody made up in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were incubated for 3 h at room temperature with the goat anti-chicken antibody conjugated with Alexa Fluor® 488 (Abcam 150169) at a concentration of 1:500 in PBS-blocking buffer ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The cultures subsequently underwent 3 cycles of 10 min PBS washes and incubated for 40 min with the appropriate secondary antibodies (Abcam or Jackson) and DAPI ...
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bioRxiv - Immunology 2022Quote: ... albicans hyphae for 3 hours and then labelled with anti-histone H3 citrulline R2+R8+R17 (Abcam, Cambridge, MA; 0.014 mg/ml) followed by donkey anti-rabbit IgG Cy3 (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times in PB and were mounted on a glass slide in the mounting medium with DAPI (Abcam ab 104139). The slides were imaged at 20X on a Zeiss LSM 880 airyscan confocal microscope.
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bioRxiv - Molecular Biology 2023Quote: ... 100-200 μg of precleared chromatin was incubated at 4°C for three hours with magnetic beads that had been prebound to one of the following antibodies: 3 μg of α-V5 antibody (Abcam ab9116), 1.5 μg of α-H3K9me2 antibody (Abcam ab1220) ...
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bioRxiv - Physiology 2022Quote: ... washed with PBS-T 3 times for 10min and incubated O/N at 4°C with primary antibody (Rabbit anti-Calnexin, Abcam, Cat# ab133615) at a dilution of 1:1000 in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... samples were blocked in PBTx (1% Triton X-100 in PBS) containing 3% bovine serum albumin and incubated over three nights with chicken anti-GFP antibody (Abcam Cat#ab13790). After several washes in PBTx ...
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bioRxiv - Microbiology 2023Quote: ... bound complexes were isolated by overnight precipitation of the pre-cleared chromatin with 100 µL of blocked beads in combination with 3-4 µg of antibodies (anti-Histone H3 (Abcam ab17913, µg), Histone H3Ac (Millipore 17-615 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the sections were washed 3 more times in PBS followed by incubation in the secondary antibody (antirabbit Alexa Fluor-488, Abcam; Sydney, Australia) diluted in blocking solution (1:250 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were washed 3 times with 1X PBST before adding anti-Rabbit secondary antibody (Catalog No: ab150080, Abcam, Waltham, MA, USA) for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... The resulting lysates was quantified using cytokine specific ELISA kits (IL-18 Thermo Fisher #BMS618-3 and IL-18 BP Abcam # ab254509), and absorbance was measured on the Tecan Spark 10M (Tecan ...