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Citations for abcam :
5701 - 5750 of 7955 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... SUMO (two antibodies used; mouse anti-SUMO-1, clone 21C7, Zymed, #33-2400, 1:200; rabbit anti-SUMO-1, Abcam, ab32058, 1:200), rabbit anti-DAXX (polyclonal D7810 ...
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bioRxiv - Molecular Biology 2022Quote: ... and then probed with primary antibody (mouse monoclonal anti-His from Pierce, 1:3000; rabbit monoclonal anti-β-Pol from Abcam, 1:2000) overnight ...
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bioRxiv - Cancer Biology 2024Quote: Immunofluorescence (IF) was performed on whole FFPE 5-µm-thick tissue sections using anti-TP63 mouse monoclonal antibody (1:100; Abcam, catalog no. ab735), anti-CK8 rabbit polyclonal antibody (1:100 ...
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bioRxiv - Pathology 2024Quote: ... Primary antibodies and labels used for wholemount staining in humans included mouse monoclonal anti-CD31 (1:50) and rabbit polyclonal anti-osteopontin (SPP1, Abcam, ab8448, 1:100). Rabbit polyclonal anti-osteopontin SPP1 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-Lgl (SCBT, rb 1:250), anti-aPKC (SCBT, rb 1:100), anti-Nrv (DSHB, mouse 1:100) and anti-GFP (AbCam, chicken, 1:1000). Midguts were washed 3Xs ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were incubated with the following primary and secondary antibodies in PBS/10 % FBS for 1 hour to overnight: mouse anti-MRE11 (abcam, #ab214, 1:500), rabbit anti-TFAM (antibodies-online ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Microbiology 2023Quote: ... Midguts were washed five times via direct tube transfer in PBS+T before staining with the secondary antibody (Goat anti-mouse Alexa Fluor 594, Abcam, Cambridge, United Kingdom) at a 1:1000 dilution in blocking solution for 1 hour at room temperature ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated at room temperature for 1 hour with mouse monoclonal primary antibodies against rat substance P (1:300 dilution, ab14184, Abcam, Cambridge, MA, USA) or normal mouse serum (ab7486 ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-Sc35 (MilliporeSigma, S4045, 1:1000) and incubated with the secondary antibodies using Alexa 488-labeled goat anti-mouse IgG (Abcam, Ab150113, 1:1000), Alexa 594-labeled goat anti-rabbit IgG (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... in PBS-Tween (1:250, overnight, 4°C) and FITC anti-mouse alpha muscle actin (Abcam, ab8211-500, 1:100, overnight, 4°C). Tissues were washed with PBS-Tween before incubation with secondary antibody PE-Goat anti-Rabbit IgG (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... sections were mounted on slides and stained using a monoclonal mouse anti-muscarinic acetylcholine receptor M4/CHRM4 primary antibody (1:500, abcam, ab77956, RRID: AB_1566454), with blocking reagents and secondary anti-mouse biotinylated antibody from the M.O.M ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were blocked in 1% BSA in PBS for 30 minutes at room temperature before overnight incubation at 4°C in primary antibodies: α-mouse CENP-A (1:1000 Abcam ab13939 (discontinued) or 1:1500 Enzo ADI-KAM-CC006-E ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated with a secondary antibody diluted in 1%BSA PBS-0.05%Tween 20 (goat anti-mouse IgG H&L – Alexa fluor 488 preabsorbed, Abcam, cat# ab150081; 1:500) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
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bioRxiv - Genetics 2023Quote: ... The following primary antibodies were used at the indicated dilutions: mouse α-H3K4me3 (1:500, MAB Institute Inc Wako 305-348191) and rabbit α-H3K9ac (1:1000, Abcam ab10812). The secondary antibodies from Jackson ImmunoResearch Laboratories (West Grove ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysates were boiled in SDS-PAGE loading buffer for 10 min and then subjected to SDS-PAGE and probed with the following specific antibodies: mouse anti-PrP monoclonal antibody 8H4 (Abcam ab61409, 1:5,000), mouse anti-MyHC antibody MF-20 (Developmental Studies Hybridoma Bank MAB4470-SP ...
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bioRxiv - Systems Biology 2023Quote: ... the cells underwent an additional 30-minute blocking step using 1× blocking buffer and were then incubated for 1 hour with secondary antibodies – Goat Anti-Mouse IgG H&L (Alexa Fluor® 594, Abcam, Cambridge, UK), and Phalloidin-iFluor 488 Reagent (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... for 10 min and blocked in 0.5% BSA in 0.5% Tx-PBS for 1 h at room temperature before incubation with mouse monoclonal anti-ψ-tubulin antibody (Abcam, ab11316, 1:1000) in 0.5% BSA in 0.5% Tx-PBS overnight at 4 ºC ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were washed with PBS and incubated in the blocking solution containing goat anti-mouse IgM Alexa Fluor 488 (Abcam, ab150121, 1:500) antibody and goat anti-mouse IgG H&L Alexa Fluor 594 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...
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bioRxiv - Developmental Biology 2023Quote: ... was used as a positive control to confirm a successful experiment and a secondary antibody anti-Mouse IgG (Abcam, ab46540, 1:50 dilution) was used as negative control to remove background in the following sequencing analysis.
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315, Abcam, Cat.#ab6789, respectively). For HisTag purification ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was done for 1 h at RT with the following horseradish peroxidase (HRP)-coupled antibodies: HRP-conjugated goat anti-mouse IgG (Abcam Cat# ab6789, RRID:AB_955439), HRP-conjugated goat anti-rabbit IgG (Abcam Cat# ab6721 ...
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bioRxiv - Cell Biology 2024Quote: ... Goat anti-rabbit IgG (Alexa Fluor 488) antibody (ab150081) and goat anti-mouse IgG (Alexa Fluor 594) antibody (ab150120) were from Abcam (Cambridge, MA, USA). SAG (Smoothened Agonist ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were purchased from the manufacturers described: mouse anti-tau antibody (tau-5; Cat# ab80579) and rabbit anti-GAPDH antibody (Cat# ab181602) from Abcam (Cambridge, United Kingdom), rabbit anti-tau polyclonal antibody (Cat# A0024 ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked in 10% chicken sera in 5% bovine serum albumin (BSA)/ phosphate buffered saline solution (PBS) for 30 minutes and stained with a rabbit anti-mouse β-actin antibody (ab8227, ABCAM, Cambridge, UK) at 1µg/ml in 1%BSA/PBS ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed the next day three times in DPBS and incubated 1h RT with secondary antibodies (rabbit anti-mouse IgG AlexaFluor 488 (Abcam catalog number ab150125), 1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... the membranes were incubated with secondary antibodies conjugated with fluorescence dyes (anti-mouse IRDye® 800CW, 1:5000, Abcam, ab216774; anti-rabbit IRDye® 680RD, 1:5000, Abcam, ab216779) at room temperature for 3 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were stained using the TUNEL Assay Kit BrdU-Red (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The positive antibody signal was revealed using DAB Substrate Kit (Abcam, ab64238). After staining in Harris hematoxylin solution (2 minutes ...
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bioRxiv - Cell Biology 2020Quote: Total histone protein was extracted using Histone Extraction kit (Abcam, Catalogue#:ab113476) according to the manufacture’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Plasma renin activity was measured using a fluorometric Renin Assay Kit (Abcam). Plasma renin activity was measured via cleavage of a fluorogenic substrate over 60 minutes at 37 degrees C and reported as renin concentration equivalent (ng/mL ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Collagen content in the skin was measured using the Hydroxyproline Kit (Abcam). All animal protocols were approved by the Institutional Animal Care & Use Committee at the University of Michigan.
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bioRxiv - Cell Biology 2022Quote: ... Threonine contents were measured with threonine assay kit (ab239726, Abcam, MA, USA) with normalization with total protein concentration (BCA assay ...
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bioRxiv - Neuroscience 2020Quote: ... and cAMP concentration was measured using a direct immunoassay kit (Abcam, MA) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: Proteasomal activity was measured using a Proteasome Activity Assay kit (Abcam, ab107921). 293T cells were transfected with indicated plasmids for 48 h and siRNA for 72 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Visualization was done by using the AEC Substrate Kit (abcam, Berlin, Germany) for 10-20 min ...
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bioRxiv - Cell Biology 2021Quote: Conditions for SA-β-galactosidase staining using Senescence detection kit (Abcam, ab655351) were described previously (Bacevic et al ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Measurement of LDH activity was performed using LDH assay kit (Abcam, ab102526) according to the manufacturer’s instructions in supernatants of iPSC-CM cultures after 7 days of drug treatment and after subsequent 7 days of drug washout ...
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bioRxiv - Microbiology 2021Quote: ... 1% SDS) and protein quantification with a bicinchoninic acid assay kit (Abcam). For each condition ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoreactive bands were developed and detected by chemiluminescence (ECL imager kit, Abcam) and the images were captured by X-ray film or myECL Chemidoc Imager (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: LDL uptake capacity was measured with the LDL Uptake Assay Kit (Abcam). Cells were incubated with 1:100 human LDL conjugated to DyLight™ 550 in Hepatozyme complete medium for 3 hours at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Picro Sirius Red stain Kit (Catalog no. ab150681) from Abcam (Cambridge, UK) was used according to manufacturer instructions to visualize collagen distribution ...
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bioRxiv - Cell Biology 2022Quote: LDH release was detected by using an LDH assay kit (Abcam, ab56393). The cell culture plates were centrifuged at 480 g for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Capture and detection antibodies were labeled using Biotin conjugation kit (Abcam, ab201796) and Alexa flour antibody labeling kits (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The EdU staining proliferation kit was used according to manufacturer’s recommendations (Abcam). Briefly ...
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bioRxiv - Microbiology 2022Quote: Iron quantification was done using the Iron Assay Kit (Colorimetric) (Abcam, UK) as per manufacturer’s instruction ...