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Citations for abcam :
5651 - 5700 of 7955 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated overnight at 4 °C with mouse Anti-GABAAR γ2 primary antibody (1:500; catalog no. ab307231, Abcam, United Kingdom) in a permeable buffer containing 2% donkey serum ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibodies (AF488 goat anti-mouse IgG, Abcam catalog #ab150113; AF594 goat anti-rabbit, Abcam catalog #ab150080; AF680 goat anti-rat IgG, Abcam catalog #ab175778) were diluted 1:500 in DPBS with BSA and Triton-X100 and incubated for 75 minutes at room temperature in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoprecipitation of mGluR2 was performed overnight at 4°C using a mouse monoclonal anti-mGluR2 N-terminal antibody (Abcam, Catalog no. ab15672), followed by RNA isolation ...
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bioRxiv - Neuroscience 2024Quote: ... 0.4% Triton X) before being stained overnight at 4C with the following primary antibodies: mouse anti-APC (CC1) (Abcam #ab16794, 1:100), goat anti-CCL11 (R&DSystems #AF-420-SP ...
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bioRxiv - Neuroscience 2024Quote: ... in TBS (pH 8) with 0.05% Tween-20 (TBS-T) and then incubated with the appropriate primary antibody (FTO (mouse, Abcam – ab92821, 1:5000), METTL3 (rabbit ...
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bioRxiv - Cancer Biology 2024Quote: ... for 60 min at 37 °C in a preheated humidity chamber and then incubated with mouse anti-HLA-E (clone: MEM-E/02, Abcam, 1:200) and rabbit anti-NKG2A (clone ...
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bioRxiv - Zoology 2024Quote: ... Samples were incubated with the primary antibody in 1% BSA in PBS overnight and then incubated during 1 hour at room temperature with a secondary antibody goat anti-mouse IgG H&L Alexa Fluor 488 (1:500; Abcam, Cat# ab150113) diluted in 1% BSA and then rinsed in PBS ...
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bioRxiv - Cancer Biology 2024Quote: Acetone-fixed cryosections of xenografted mouse tumors were stained with rabbit polyclonal anti-Ki-67 (1:50 dilution, ab15580, Abcam, Cambridge, UK) antibody diluted with DAKO diluent (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were cut in a way to allow separate incubation with anti-ERM (1:1000, #3142, cell signaling) anti-pERM (1:1000, #3141, cell signaling) and anti-GAPDH (1:10.000, ab125247, abcam) antibodies ...
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bioRxiv - Immunology 2021Quote: ... Proliferation of cells was measured staining of control and Irradiated UVC with either 2 μM Cell Trace Yellow (Abcam) according to kit protocol and analyzing the dilution of the dye at 24-hour periods over 3-days and measuring fluorescence intensity ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells are resuspended as a single-cell suspension at densities of 750K-1M cells/mL in mTeSR1 containing 10μM ROCK inhibitor Y27632 (Abcam). 200μL of cell suspension is then pipetted onto prepatterned dishes and allowed to settle for 15 minutes before adding 1mL of mTeSR1 and allowing cells to settle for 10 additional minutes ...
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bioRxiv - Biophysics 2023Quote: We achieved actin caps inhibition in endothelial cells by submerging cells in 30nM to 240nM Latrunculin B (LatB, Abcam) dissolved in DMSO solution (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... the cells were blocked for 1 h at room temperature using an unconjugated goat anti-mouse IgG H&L antibody (polyclonal, 1:1000, Abcam ab6708, Cambridge, UK) diluted in previously mentioned blocking solution ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the primary antibody (monoclonal mouse anti-vinculin, 1:100, Sigma V9264, St. Louis, USA; polyclonal rabbit anti-talin 1, 1:100, Abcam ab71333, Cambridge, UK) diluted in blocking solution for 1.5 h at room temperature or overnight at 4 °C.
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bioRxiv - Cell Biology 2020Quote: ... the primary rabbit mono-specific antibodies were diluted into a concentration of 2-4 µg/ml in blocking buffer (PBS + 4% FBS) containing 1 µg/ml mouse anti-alpha-tubulin (Abcam, ab7291, Cambridge, UK) and chicken anti-KDEL respectively (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... This was directly followed by incubation overnight at room temperature with primary antibodies (GFAP; host: mouse; diluted 1:1000; Sigma-Aldrich; Cat# MAB360. GFP; host: chicken; diluted 1:2000; Abcam; cat# ab13970) diluted in antibody solution (ABS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the samples were incubated with Alexa Fluor 488- or 594-conjugated goat-anti-mouse or goat-anti-rabbit secondary antibodies (Abcam, 1:500 dilution) overnight at 4 °C and rinsed several times in PBST before mounting in ProLong Diamond Antifade mountant (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Afterward the sections were incubated with 1:100 diluted mouse polyclonal anti-GFAP (Cusabio, Houston, TX, USA) and 1:500 diluted rabbit polyclonal anti-HuC/D (Abcam, Cambridge, United Kingdom) overnight at 4°C· followed by incubation with Cy3-labeled goat anti-mouse IgG (Beyotime ...
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bioRxiv - Cell Biology 2021Quote: ... and for >16 hrs at 4°C with the following primary antibodies: mouse-α-CD63 (1:1000, clone TS63; Abcam, Cambridge, United Kingdom), mouse-α-Flotillin-1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... so membranes were also incubated with a mouse monoclonal anti-beta actin primary antibody (dilution: 1:5000, product number: ab8226, Abcam, Cambridge, MA, USA). After TBST washing three times for 10 minutes each wash ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-CaVβ3 (Alomone Labs #AAC-008, 1:800) or mouse anti-CaVβ4 (Neuromab #75-054, 1:1000) and rabbit anti-β-tubulin (Abcam #ab6046, 1:600,000). Membranes were then incubated in LiCor Blocking Buffer as described above and LiCor IRDye secondary antibodies ...
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bioRxiv - Physiology 2022Quote: Both neonatal and adult ducts were stained using antibodies against the cholangiocyte marker keratin 19 (K19, 1:10, Developmental Studies Hybridoma Bank, TROMAIII) and for the myofibroblast marker α-smooth muscle actin (mouse α-SMA, Abcam ab7817). Adult bile ducts were also stained for hematoxylin and eosin and Masson trichrome.
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bioRxiv - Neuroscience 2022Quote: ... FoxP2 (mouse, Merck Millipore: MABE415, RRID: AB_2721039; 1:2000; Invitrogen: MA5-31419, RRID: AB_2787055; 1:2000; rabbit, Abcam: ab16046, RRID: AB_2107107; 1:500), Calb1 (rabbit ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies: mouse anti-HSP70 (ab2787, Abcam, Cambridge, MA, USA), rabbit anti-TSG101 (14497-1-AP ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min with primary antibodies diluted in blocking solution as follows: mouse anti-Pyruvate dehydrogenase E2/E3 (PDHE2/E3, ab110333, Abcam, 1 μgmL−1); mouse anti-clathrin heavy chain (X22 ...
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bioRxiv - Cell Biology 2022Quote: ... We used mouse anti-beta-tubulin (E7, Developmental Studies Hybridoma Bank, 1:300 dilution) and rabbit anti-histone H3 (Abcam, 1:500 dilution). Embryos were then washed 4 x 2 h in PBT and incubated overnight at 4°C in PBT supplemented with goat anti-mouse and goat anti-rabbit secondary antibodies coupled to Alexa Fluor 488 and 568 (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... in a Tris-glycine-methanol transfer buffer overnight and detected with the following antibodies: 1:5000 monoclonal mouse anti-Hsp70 [C92F3A-5] (Abcam Cat# ab47455, RRID:AB_881520) (abcam) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... e cells were then incubated with primary antibodies against mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) (1:200)(ab48508, 1:200 dilution; Abcam, Cambridge, United Kingdom) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used and dilution ratios are: mouse monoclonal anti-Acetylated α-Tubulin (1:500, ab24610, clone 6-11B-1, abcam, Cambridge, MA), mouse monoclonal anti-Acetylated α-Tubulin (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... stained and incubated in a humidified chamber with rabbit anti-mouse Collagen I overnight at 4°C (1:100 dilution Abcam Catalog No. ab34710); washed 3 times in TBS-Tween (.05% Tween ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at room temperature and were incubated overnight at 4 °C with primary rabbit polyclonal antibodies against mouse MAGL (Abcam, Cambridge, UK; ab180016) or human MAGL (LSBio ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed and incubated with the appropriate fluorescein-conjugated secondary antibodies (Alexa Fluor 594-labeled goat anti-mouse IgG from Abcam for γ-H2AX; Alexa Fluor 488-labeled goat anti-rabbit IgG from Abcam for RAD51). After washing ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113; 1:1000 Goat-α-rabbit AlexaFluor555, abcam ab150090) and washed again in PBS (4×10 min) ...
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bioRxiv - Developmental Biology 2020Quote: ... cover slips were incubated in primary antibody at 4°C overnight followed by rinses in PBS and one hour incubation with goat anti-mouse IgG (Alexa Fluor 647-conjugated) secondary antibody (Abcam, Cambridge, MA, ab150115) and/or goat anti-rabbit IgG (Alexa Fluor 594-conjugated ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 9 was performed using citrate buffer and sections were incubated with the primary antibody rabbit anti‐mouse CD31 (1:50) (ab28364, Abcam, Cambridge, MA, USA) at 4°C overnight ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 6 with citrate buffer and the primary antibody rabbit anti-mouse CD11b (1:200) (ab133357, Abcam, Cambridge, MA, USA) and antigen retrieval with 1:20 proteinase K/TE buffer and rat anti-mouse F4/80 (1:100 ...
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bioRxiv - Biochemistry 2020Quote: ... blots were incubated overnight at 4°C with a mouse antibody against the α subunit of Na+/K+-ATPase (Abcam-ab7671, 1:2,000) and with a mouse antibody against the His-tag (Tetra·His Antibody ...
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bioRxiv - Cell Biology 2020Quote: ... with fibronectin-depleted (depleted via column purification using gelatin sepharose) FBS at 10% on glass coverslips coated with 10ug/mL murine fibronectin (native mouse fibronectin, Abcam, Cambridge MA, USA) or laminin (laminin mouse protein ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then probed with Alexa Fluor anti-rabbit A680 secondary antibody and Alexa Fluor anti-mouse A790 secondary antibody (Abcam ab175773 and ab175783). Membranes were visualized using an Odyssey Infrared Imaging System (LI-COR) ...
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bioRxiv - Biochemistry 2021Quote: ... the chambered coverglass plates were rinsed three times with PBS and cells were labeled with fluorescently conjugated secondary antibody (anti-Rabbit Alexa Fluor 488, lnvitrogen #A-11008, 1:500; anti-Mouse Alexa Fluor 647, Abcam# ab150119, 1:500) in blocking buffer for one hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... then incubated in 5% milk in TBS-T containing 1:10000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... Western blots were imaged on an Odyssey imaging system (Licor) with anti-PAR primary and goat anti-mouse IR680 (Millipore Sigma AM80 and Abcam ab216776, respectively). A dT50 oligo was radioactively labeled with 32P by T4 PNK (NEB M0201) ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA fibers were incubated with mouse-FITC-anti-bromodeoxyuridine (detects IdU, B44, Becton Dickinson, 1:50) and rat-anti-bromodeoxyuridine (detects CldU, abcam ab6326, 1:100), at 37°C for 1 hour and then with anti-mouse IgG AlexaFluor 488 (Invitrogen A11029 ...
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bioRxiv - Microbiology 2020Quote: ... the fixed cells were labeled with primary antibodies including goat anti-major outer-membrane protein (MOMP; Meridian, Memphis, TN) and mouse or rabbit anti-six histidine tag (Genscript, Nanjing, China and Abcam, Cambridge, UK, respectively). For experiments using CRISPR interference ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with matching secondary antibodies (anti-rabbit and anti-guinea pig were the same ones as previously used as well as an Alexa Fluor goat anti-mouse 555 [Abcam, product code: ab150114]) and a DAPI stain was added ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated at room temperature for 1 hour with mouse monoclonal primary antibodies against rat substance P (1:300 dilution, ab14184, Abcam, Cambridge, MA, USA) or normal mouse serum (ab7486 ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-Sc35 (MilliporeSigma, S4045, 1:1000) and incubated with the secondary antibodies using Alexa 488-labeled goat anti-mouse IgG (Abcam, Ab150113, 1:1000), Alexa 594-labeled goat anti-rabbit IgG (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... in PBS-Tween (1:250, overnight, 4°C) and FITC anti-mouse alpha muscle actin (Abcam, ab8211-500, 1:100, overnight, 4°C). Tissues were washed with PBS-Tween before incubation with secondary antibody PE-Goat anti-Rabbit IgG (Abcam ...