Labshake search
Citations for PacBio :
301 - 338 of 338 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... a SMRTbell DNA library was constructed according to the Pacbio HiFi SMRTbell protocol using SMRTbell Express Template Prep Kit 3.0 (Pacbio, 102-182-700). After BluePippin size selection (Sage Science ...
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bioRxiv - Genomics 2023Quote: ... The DNA was then selected for high molecular weight DNA using the PacBio Short Read Eliminator kit (PacBio, Menlo Park, CA, USA). Concentration was assessed using the dsDNA BR assay on a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Systems Biology 2024Quote: ... A HiFi sequencing library for de novo genome assembly was prepared using the SMRTbell library prep kit (PacBio, Menlo Park, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... and constructed PacBio SMRTbell library (∼20 kb) for PacBio Sequel using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) following the manufacturer recommended protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing primer v4 was annealed to the Iso-Seq library pool and complexed to the sequencing polymerase with the Sequel II binding kit v2.1 (PacBio, 101-843-000). Calculations for primer to template and polymerase to template binding ratios were kept at default values for the library type ...
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bioRxiv - Genomics 2024Quote: ... The final library preparation for sequencing was conducted using The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) The library was loaded on-plate in diffusion mode at concentration of 90 pM followed by sequencing on the Pacific Biosciences SEQUEL IIe System at The Chinese University of Hong Kong under the following conditions ...
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bioRxiv - Bioengineering 2024Quote: High molecular weight genomic DNA was isolated from NHP liver tissues using Nanobind Tissue kit from PacBio (Cat. No. 102-302-100) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Sheared DNA was treated with exonuclease to remove single-stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). Libraries were purified with AMPure PB beads (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: A long-read sequence library was constructed using an SMRTbell Express Template Prep Kit 2.0 and sequenced on SMRT cells (1M v3 LR) in a PacBio Sequel system (PacBio, Menlo Park, CA, USA). The obtained reads (≥15 kb ...
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bioRxiv - Genomics 2021Quote: The manufacturer recommended protocol was used to generate a PacBio SMRTbell library (~20kb) for PacBio Sequel using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio ...
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bioRxiv - Genomics 2022Quote: ... The sheared DNA was used for HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The resultant library was separated on BluePippin (Sage Science ...
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bioRxiv - Genomics 2022Quote: ... were added to ligation products following an adapted version of the PacBio Microbial Multiplexing PacBio protocol (Part Number 101-696-100 Version 07 (July 2020)) using the PacBio SMRTbell Express Template Prep Kit 2.0 (PacBio, PN: 100-938-900) as follows ...
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bioRxiv - Genomics 2022Quote: ... PacBio HiFi reads were generated by first constructing a ~20kb library using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2022Quote: ... The sheared and purified DNA fragments were used as templates to prepare the SMRTbell library through SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... All samples were depleted of short DNA fragments (less than 10kb long) with the short read eliminator (SRE) kit (Circulomics, SS-100-101-01; now PacBio 102-208-300) or the SRE XS kit (Circulomics SS-100-121-01 ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was then prepared by following the protocol of the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700). Briefly ...
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bioRxiv - Genomics 2023Quote: ... They constructed the PacBio SMRTbell library (∼20kb) for PacBio Sequel using SMRTbell Express Template Prep Kit V 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (~20kbp mean insert length) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20 kbp mean insert length) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... Sheared DNA was treated with exonuclease to remove single stranded ends and DNA damage repair mix followed by end repair and ligation of barcoded blunt adapters using SMRTbell Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). Libraries were purified with AMPure PB beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20 kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then pooled all the barcoded libraries to be multiplexed and purified the pooled library using the AMPure PB Kit (PacBio # 100-265-900). We used a concentration of 0.6X of AMPure Beads and eluted the DNA in 37 µl of elution buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we used 80 ng of each library in the end-repair/ligation reaction using barcoded adaptors from the SMRTbell barcoded adapter complete prep kit -96 (PacBio # 100-514-900). We then pooled all the barcoded libraries to be multiplexed and purified the pooled library using the AMPure PB Kit (PacBio # 100-265-900) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we performed a DNA damage repair reaction followed by exonuclease treatment (ExoIII and ExoVII) using the SMRTbell DNA Damage Repair Kit (PacBio # 100-486-900) according manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: The HiFi SMRTbell library was constructed using the SMRTbell gDNA Sample Amplification Kit (Pacific Biosciences [PacBio], Menlo Park, CA; Cat. #101-980-000) and the SMRTbell Express Template Prep Kit 2.0 (PacBio Cat ...
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bioRxiv - Genomics 2024Quote: ... and the sheared DNA was used for HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The obtained DNA libraries were fractionated with BluePippin (Sage Science ...
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bioRxiv - Genomics 2024Quote: ... PacBio Sequencing primer is then annealed to the SMRTbell template library and sequencing polymerase was bound to them using Sequel II Binding kit 2.0 (PacBio Catalog #101-789-500). The prepared SMRTbell template libraries are then sequenced on a PacBio Sequel IIe sequencer using SMRT Link 10.2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using the Qiagen kits Dneasy (for short-read sequencing; from approximately 5000-7000 rotifers) and GenomicTips 100 (for long read Pacbio sequencing; from >20000 rotifers), and RNA was extracted from freshly prepared biomass with Rneasy.
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bioRxiv - Neuroscience 2020Quote: ... PacBio library preparation was performed with 500 ng of amplified cDNA using SMRTbell Express Template Prep Kit V2.0 (PacBio cat#PN: 100-938-900) to obtain Sequel II compatible library complex and was sequenced on a total 24 Sequel I SMRTcells with a run time of 10 hours and 20 Sequel II SMRTcells with a run time of 30 hours across samples and replicates.
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bioRxiv - Genomics 2020Quote: ... The three cDNAs were pooled in equimolar ratios and used to prepare a SMRTbell™ library using the PacBio Template Prep Kit (PacBio, Menlo Park, CA). The SMRTbell™ library was then sequenced on a Sequel v2 SMRT cell with polymerase 2.1 and chemistry 2.1 (P2.1C2.1 ...
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bioRxiv - Microbiology 2024Quote: Extraction of high molecular weight DNA from overnight cultures of CPs was accomplished using the Nanobind CBB kit (PacBio catalog no.102-301-900). Barcoded sequencing libraries were prepared using the SMRTbell prep kit 3.0 (PacBio catalog no.102-141-700 ...
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bioRxiv - Cancer Biology 2023Quote: Tumour genomic DNA was extracted from fresh-frozen tissue with the Nanobind tissue kit (Circulomics NB-900-701-01, now PacBio SKU 102-302-100) and Germline DNA from blood with the Nanobind CBB kit (Circulomics NB-900-001-01 ...
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bioRxiv - Genomics 2023Quote: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 (Pacific Biosciences -PacBio, Menlo Park, CA, Cat. #100-938-900) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Short read elimination was performed using the Circulomics Short Read Eliminator kit (formerly SS-100-101-01, now SKU 102-208-300; PacBio, Menlo Park, California, USA). The size selected DNA was quantified using a Qubit 1.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... a fraction of short fragments was removed from 9 μg of total DNA using the Short-Read Eliminator Kit XL (PacBio, SKU 102-208-400), according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... Libraries were pooled in an equimolar approach prior to construction of SMRTbell® Prep Kit 3.0 (Catalog number 102-182-700, PacBio, Menlo Park, CA, USA) sequencing library preparation according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 following the manufacturer’s protocols (Pacific Biosciences - PacBio, Menlo Park, CA; Cat. #100-938-900). HMW gDNA was sheared to a target DNA size distribution between 15-20 Kb and concentrated using 0.45X of AMPure PB beads (PacBio Cat ...