Labshake search
Citations for PacBio :
251 - 300 of 338 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... method and used for the construction of a SMRTbell library using the SMRTbell Express Template Prep Kit 2.0 (PacBio). The library was sequenced with the circular consensus sequencing (CCS ...
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bioRxiv - Genomics 2024Quote: ... the DNA was used to generate PacBio HiFi libraries via the SMRTbell prep kit 3.0 (PacBio, 102-182-700). Size selection was performed either with diluted AMPure PB beads per the protocol ...
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bioRxiv - Genomics 2024Quote: ... The Kinnex Iso-Seq library was prepared by first synthesizing cDNA from 300 ng of total RNA using Iso-Seq express 2.0 kit (PacBio) and the size distribution of the resulting cDNA was checked with a Bioanalyzer (Agilent) ...
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bioRxiv - Genetics 2024Quote: ... Sequencing primers and polymerase were bound to each library pool using the Sequel® II Binding Kit 3.2 (PacBio). Polymerase-bound SMRTbell complexes were purified ...
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bioRxiv - Genomics 2021Quote: A long-read sequence library was prepared using the SMRTbell Express Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA). The size selection of the library was performed by BluePippin (Sage Science ...
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bioRxiv - Neuroscience 2021Quote: ... The SMRT sequencing was performed on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) using the Clontech SMARTer PCR cDNA Synthesis Kit (recommended by Pacbio). Multiple parallel PCR reactions were set after the first strand synthesis employing the PrimeSTAR GXL DNA Polymerase kit ...
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bioRxiv - Microbiology 2021Quote: ... to 15 kb and size-selected using the Blue Pippin (Sage Science, USA) and prepared for sequencing using SMRTbell Express Template Preparation Kit 1.0 (PacBio, USA) according to manufacturers’ instructions ...
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bioRxiv - Genomics 2022Quote: ... Sequencing libraries were prepared for each species using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and following the ultra-low protocol ...
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bioRxiv - Genomics 2020Quote: ... The two fractions were recombined at equimolar quantities and library preparation performed using SMRTbell Template Prep Kit v1.0 (PacBio, USA). Sequencing was performed on the PacBio Sequel 1M SMRT cell ...
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bioRxiv - Genomics 2022Quote: ... A linked-read sequencing library was prepared using the 10X Genomics Chromium Genomic Kit (from the same DNA extraction as used for PacBio sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries were prepared and multiplexed with the PacBio SMRTbell® Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) according to the published protocol (19) ...
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bioRxiv - Molecular Biology 2023Quote: High molecular weight DNA was extracted from frozen pellets (1 million cells) using the Nanobind HMW DNA Extraction kit (PacBio) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... a long-read sequence library was prepared using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and sequenced using a PacBio Sequel II system (PacBio ...
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bioRxiv - Genomics 2024Quote: ... DNA samples for Oxford Nanopore or PacBio long read sequencing were enriched for HMW DNA (> 25 kb) employing the Short Read Eliminator Kit (Circulomics/PacBio). Quality control was performed by determining A260/A280 and A260/A230 ratios on NanoDrop ...
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bioRxiv - Genomics 2024Quote: A final library preparation was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100) before sequencing ...
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bioRxiv - Genomics 2023Quote: ... DNA was pooled and sequenced at the Australian Genome Research Facility (Brisbane, Queensland, Australia) using a SMRTbell® prep kit 3.0 (PacBio), and circular consensus sequencing (CCS ...
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bioRxiv - Genomics 2023Quote: ... Extractions were performed using the Nanobind Tissue Big DNA kit following the manufacturer’s instructions (Pacific BioSciences - PacBio, Menlo Park, CA). For song sparrow ...
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bioRxiv - Plant Biology 2022Quote: ... and a HiFi SMRTbell library was constructed with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library DNA was fractionated using a BluePippin (Sage Science ...
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bioRxiv - Genomics 2024Quote: ... DNA was then isolated from the washed blood pellet using the Circulomics Nanobind CBB kit and protocol according to the manufacturer’s recommendations (Circulomics, now PacBio company). High molecular weight DNA was eluted from the Nanodisk with 150µl Tris-cl buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A PacBio SMRTbell library was prepared using the SMRTbell Template Prep Kit 2.0 and Sequel II Binding 2.2 (PacBio, CA, USA), and loaded onto the sequencer by diffusion ...
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bioRxiv - Molecular Biology 2024Quote: High molecule weight DNA was extracted from patient’s HSPC with the Nanobind CBB kit (PacBio, catalog no. 102-301-900) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The PacBio SMRTbell library (15-20kb) was constructed using SMRTbell Express Template Prep Kit 3.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... All samples were depleted of short DNA fragments (less than 10kb long) with the SRE kit (Circulomics, SS-100-101-01, now PacBio) or the SRE XS kit (Circulomics SS-100-121-01 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The resulting nuclei/cellular compartment mix was used as input for the Nanobind plant nuclei big DNA kit from PacBio. Steps were followed as listed in the kit protocol except for large cell inputs ...
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bioRxiv - Genomics 2021Quote: ... The size-selected DNA was prepared into a PacBio library using the SMRTbell® Express Template Preparation Kit 2.0 (PacBio, USA), then sequenced across two PacBio Sequel II 8M SMRT cells (PN:101-389-001) ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries for each sample were created using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Cat. No. 100-938-900) per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genetics 2023Quote: HiFi SMRTbell libraries were constructed according to the manufacturer’s manual by using SMRTbell Express Template Prep Kit 2.0 (PacBio, 100-938-900). For all samples ...
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bioRxiv - Cancer Biology 2023Quote: ... and Germline DNA from blood with the Nanobind CBB kit (Circulomics NB-900-001-01, now PacBio SKU 102-301-900). After extraction ...
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bioRxiv - Immunology 2023Quote: ... PacBio SMRTbell amplicon libraries for PacBio Sequel were constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... using settings 28/31 and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio, 102-182-700). Size selection was performed with Pippin HT using a high-pass cutoff of 17 kbp (Sage Science ...
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bioRxiv - Evolutionary Biology 2023Quote: ... elegans female of each genotype (Ao, Io, oo), using the Nanobind® Tissue Big Extraction Kit (Cat. No. NB-900-701-01, Circulomics Inc. (PacBio), MD ...
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bioRxiv - Microbiology 2024Quote: ... Samples with many short DNA fragments were further purified using either the Short Read Eliminator kit (PacBio, San Diego, CA, USA), or 0.4 - 0.5 x volume KAPA PURE SPRI beads (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was extracted from muscle tissue that was preserved in ethanol using the Circulomics Nanobind Tissue Big DNA kit (Circulomics Inc., PacBio, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified ligation product was split into two reactions and enriched using 10-18 cycles of PCR using barcoded amplification oligos (IDT) and SMRTbell® gDNA Sample Amplification Kit (PacBio). Up to sixteen libraries were pooled in equimolar concentrations and the pooled libraries were size-selected using the 0.75% agarose gel cassettes with Marker S1 and High Pass protocol on the BluePippin (Sage Science) ...
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bioRxiv - Genomics 2024Quote: ... Fragments less than 5 kb were selectively removed using the AMPure PB beads size selection kit (Pacbio, Menlo Park, CA, USA). The SMRTbell® prep kit 3.0 from Pacbio was used to prepare the sequencing libraries ...
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bioRxiv - Genomics 2024Quote: ... A SMRTbell library was constructed for both samples using the SMRTbell® prep kit 3.0 (PacBio Cat. No. 102-141-700), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The PacBio SMRTbell library for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer-recommended protocol ...
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bioRxiv - Genomics 2020Quote: ... The polymerase-bound library was sequenced on 8 SMRT Cells with a 10 h movie time using the Sequel Sequencing Kit 3.0 (PacBio, 101-597-900) and a Sequel SMRT Cell 1M v3 (PacBio ...
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bioRxiv - Microbiology 2020Quote: ... eluted in 37 μl EB (10 mM TrisHCl pH 8.0) and processed using SMRTBellTM Template Prep kit 1.0 (PacBio Part# 100-259-100) to SMRTbell libraries ...
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bioRxiv - Genomics 2023Quote: ... The DNA was then selected for high molecular weight DNA using the PacBio Short Read Eliminator kit (PacBio, Menlo Park, CA, USA). Concentration was assessed using the dsDNA BR assay on a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... a SMRTbell DNA library was constructed according to the Pacbio HiFi SMRTbell protocol using SMRTbell Express Template Prep Kit 3.0 (Pacbio, 102-182-700). After BluePippin size selection (Sage Science ...
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bioRxiv - Genetics 2023Quote: ... UW then prepared Iso-seq libraries from this cDNA using a Iso-Seq Express SMRTbell library prep kit (PacBio #100-938-900). The resulting iso-seq libraries were then sequenced by UW on a PacBio Sequel II system using an 8M SMRT cell (v2.1 chemistry) ...
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bioRxiv - Genomics 2023Quote: The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2023Quote: HMW DNA from 1.5 x 106 cells was used to generate PacBio HiFi libraries with the SMRTbell express template prep kit 2.0 (PacBio 100-938-900). Size selection was performed with Megaruptor 2 (Diagenode ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... The purified linearized library was used to prepare a PacBio sequencing library using a SMRTbell prep kit 3.0 (PacBio #102-141-700), following the manufacturer’s recommended protocol (PacBio Protocol #102-166-600 REV02 ...
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bioRxiv - Genomics 2024Quote: ... The final preparation of the library was performed using the Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PacBio SMRTbell library (∼20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2024Quote: The final library preparation for sequencing was performed with The Sequel® II binding kit 3.2 (PacBio Ref. No. 102-194-100). In brief ...