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Citations for Promega :
251 - 300 of 5340 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Each quantitative RT-qPCR reaction was performed using the GoTaq qPCR Master Mix kit (Promega). For a 10 μl reaction ...
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bioRxiv - Cancer Biology 2019Quote: ... which were performed with the use of GoScript RT kit following the manufacturer’s instructions (Promega).
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bioRxiv - Genomics 2021Quote: ... and cDNA synthesis using the GoScript RT kit and oligo dT primer (Promega, Madison, Wisconsin). For STARR-seq samples ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by reverse transcription (RT) using M-MLV reverse transcription kit (Promega, Madison, WI, USA). Blank qPCR Master Mix kit (EURx ...
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bioRxiv - Neuroscience 2024Quote: ... using BRYT Green Dye contained in the GoTaq 1-Step RT-qPCR kit (Promega: A6020) in the CFX96 Touch Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... or AMV RT (Promega) as described in manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: pCIneo Precursor miR-146a (pmiR-146a) and HA-HuR plasmids were transfected using Fugene HD (Promega) for RAW264.7 cells as described previously (Goswami et.al ...
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bioRxiv - Genetics 2021Quote: ... Apoptosis detection was performed using the DeadEnd™ Colorimetric Apoptosis Detection System (Promega) following the manufacturer’s instructions for fixed tissue slides ...
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bioRxiv - Bioengineering 2023Quote: ... HiBiT detection was performed using the Nano-Glo HiBiT Lytic Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: A GenePrint 10 System PCR Amplification kit (Promega Corporation) was used to determine the genetic signature of both samples based on the multiplex analysis of 9 loci and the Amelogenin sex-determining marker ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the GoTaq 1-step RT-qPCR system (Promega). Knockdowns were verified using primers described in Table I ...
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bioRxiv - Bioengineering 2021Quote: PCR products containing the trigger T7 transcriptional unit were cleaned by PCR cleanup kit (Promega, A6754) and 250 ng were processed by in vitro transcription using the HiScribe T7 kit (Promega ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega) and ligated into pET17b vector using the Gibson Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Bioengineering 2021Quote: ... Primer concentrations for RT-qPCR reactions were initially evaluated by performing 25μL PCR reactions using Go Taq® Master Mix (Promega Corporation) with approximately 100μg of gDNA ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR reactions were performed using 20 ng of cDNA of WT2 and NBS8 organoids using GoTaq® DNA Polymerase (Promega). Total RNA from commercially human fetal and adult brain (BioChain® ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR (qPCR) reactions were conducted in a 10-µL total volume using a 2× GoTaq® qPCR Master Mix (Promega) and run on an ABI 7500 qPCR thermocycler (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription of viral RNA followed by quantitative PCR was performed with GoTaq® Probe 1-Step RT-qPCR System (Promega) on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ATP levels were estimated using the ATP detection reagent of the Mitochondrial ToxGlo Assay kit (Promega). 50 μg mitochondria from WT and deletion strains grown at 30°C in YPG media were lysed by two times snap freezing the mitochondria in liquid nitrogen followed by thawing on ice ...
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bioRxiv - Immunology 2021Quote: ... and pCDNA-3.1 was collected and assayed for LDH release using the Cytotoxicity Detection Kit (Promega) according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2021Quote: ... Mitochondria ATP content was quantified using the ENLITEN® ATP Assay System Bioluminescence Detection Kit (Promega, Wisconsin ...
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bioRxiv - Cell Biology 2023Quote: ... and firefly and Renilla luciferase activities were measured using the Dual-Luciferase detection kit (Promega #E1910) with a bioluminescence plate reader (Citation ...
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bioRxiv - Microbiology 2024Quote: ... cell supernatants were harvested for luciferase activity detection using Nano-Glo Luciferase Assay System kit (Promega) on Glomax Multi-Detection System (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... The fragment sizes provided by the Fragment Analyzer were compared to the degradation indices obtained from real-time quantitative polymerase chain reaction (qPCR) with PowerQuant System (Promega Corporation, Madison, WI, USA). After one year of storage in − 20 °C ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Reverse transcription (RT) was performed with the GoScript™ Reverse Transcriptase kit Random Primers (Promega, A2801) to convert 1 μg of RNA into cDNA ...
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bioRxiv - Bioengineering 2020Quote: ... amplicons from three PCR runs for each root sample were combined (Clement et al., 1998) and then purified using a PCR purification kit (PCR Clean-up Kit; PROMEGA Inc., Wisconsin, USA).
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bioRxiv - Synthetic Biology 2019Quote: ... the HiBiT detection was performed using the Nano-Glo HiBiT Lytic Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega). Knockdowns were verified using the primers ...
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bioRxiv - Genomics 2020Quote: ... 0.2 μL GoScript™ RT Mix for 1-Step RT-qPCR (Promega, Madison, WI), 0.75 μL primer/probe sets for either N1 ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega) with an input of 50 ng RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega) with an input of 50 ng RNA ...
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bioRxiv - Bioengineering 2022Quote: ... GloMax-Multi+ Detection System (Promega). After 24 h ...
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bioRxiv - Genomics 2019Quote: ... The supernatant was discarded and the pellet was resuspended in a small volume of SSC cocktail (3xSSC [Sigma; S0902], 0.04% BSA, 40mM DTT [Sigma; 43816], 0.5U/mL RNasin plus [Promega; N2615]) to reach a concentration of approximately 2000 cells/µL ...
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bioRxiv - Molecular Biology 2019Quote: ... (vi) BP4_DM2_H-(GoScript RT, Promega) consisted of 9 DM2 and 4 control samples ...
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bioRxiv - Molecular Biology 2019Quote: ... (v) BP3_DM1_H-(GoScript RT, Promega) consisted of 2 DM1 and 4 control samples ...
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bioRxiv - Microbiology 2020Quote: ... The dcas9 PCR amplicon was purified using a Wizard SV Gel and PCR Clean-up kit (Promega) and digested with AscI and HindIII ...
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bioRxiv - Synthetic Biology 2020Quote: ... Primers and remnants of the PCR reaction were removed using the Wizard PCR cleanup kit (Promega, USA). The concentration of the purified DNA was determined using an ND-2000 NanoDrop spectrophotometer ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was run on the agarose gel and purified using PCR purification kit (A1222, Promega). The Alu PCR product was cloned into using pGEM-T Easy Vector (A1380 ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplicons were purified using the Wizard® SV Gel and PCR Clean-Up System kits (Promega), followed by Sanger sequencing (Eurofins) ...
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bioRxiv - Developmental Biology 2020Quote: The coding region of her9 was amplified by RT-PCR and the cDNA was cloned into a pGEMT-easy plasmid (Promega, Madison, WI). Capped mRNA was synthesized using the mMessage T7 kit (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... all RT mixture of samples were pooled and purified with Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) according to the manufacture’s manual except for using 80% EtOH instead of Membrane Wash Solution ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were cloned using the pGEMT kit (Promega): 3 μl PCR products were ligated to 50 ng pGEMT vector in the presence of 3 units T4 ligase overnight at 18°C (final volume 10 μL) ...
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bioRxiv - Cell Biology 2019Quote: ... purified chromatin was recovered using a PCR purification kit (Promega). Sequencing libraries were generated using standard methods and samples were sequenced on a MiniSeq instrument (Illumina ...
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bioRxiv - Zoology 2019Quote: ... purified using Wizard SV Gel and PCR Cleanup kit (Promega), and sequenced directly (Sequetech ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were subject to TA cloning (kit form Promega). Plasmid DNA was extracted from individual colonies and was confirmed for deletion by Sanger sequencing.
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bioRxiv - Genetics 2023Quote: ... We used GoTaq G2 Hot Start PCR kit (Promega, M7423) with the following conditions ...
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bioRxiv - Genetics 2023Quote: ... We used GoTaq G2 Hot Start PCR kit (Promega, M7423) with the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... followed by lysis for qPCR analysis or detection of luminescence using a Firefly Luciferase Assay kit (Promega) and a Glomax luminometer (Promega) ...
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bioRxiv - Microbiology 2023Quote: NADH/NAD+ and NADPH/NADP+ ratios were measured using the Bioluminescent NAD/NADH-Glo detection kit (Promega) and NADP/NADPH-Glo detection kit (Promega) ...
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bioRxiv - Genetics 2024Quote: ... and the ATP levels were measured using the bioluminescence detection kit (Promega ENLITEN ATP Assay System, FF2000) and Spectra Max M2 multimode microplate reader (Molecular Devices) ...
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bioRxiv - Neuroscience 2023Quote: ... The revers transcription (RT) reaction was performed by M-MLV reverse transcription kit (Promega, Madison, WI, USA).